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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmiah Aplikasi Isotop Radiasi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Penelitian Tanaman Industri (Littri)
RAGAPADMI PURNAMANINGSIH
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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Karakterisasi Morfologi, Anatomi dan Fisiologi Galur Mutan Gandum yang Ditanam di Dataran Rendah Tropik Sari, Laela; Purwito, Agus; Sopandie, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Jurnal Penelitian Pertanian Tanaman Pangan Vol 35, No 1 (2016): April 2016
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.804 KB) | DOI: 10.21082/jpptp.v35n1.2016.p45-52

Abstract

Characterization of mutant wheat (Triticum aestivum L.) lines is a step on the breeding program to determine the beneficial characters for increasing the productivity in tropical lowland. The aim of this research was to obtain information on the variability of morphological, anatomical, and physiological characters that could be used as selection criteria and to obtain adaptive mutant lines of “Alibey” in tropical low altitude land. Research was conducted at the Experimental Farm of SEAMEO-BIOTROP in Bogor 250 m above sea level, from April to December 2013. Mutant lines of “Alibey” consisted of 16 M3 mutants resulted from treatments of EMS. LC50 of “Alibey” at 0.1% EMS for 60 minutes. Results showed that the mutant lines changed their morphological traits significantly, as indicated by the four characters i.e. long stem panicle (8 mutants), grain weight/panicle (1 mutant), weight of 100 seeds (4 mutants) and seed weight/plant (9 mutants). However, the mutant had no significant effect on the nine other characters, including: time of flowering, days to maturing, panicle length, plant height, number of tillers, panicle number, and leaf area. Anatomical characters namely leaf thickness and stomata size showed different values between “Alibey” mutant (AB-0.1.60-1-7-1) and the original Alibey. For the physiological characters there were significant differences among mutants with respect to the amount of proline and glucose levels. Proline level in the control plant was 4.15 ug/g BB, while that in mutant “AB-0.1.60-3-16-1” was 263.47 µg/g BB, and that in “AB-0.1.60-3-3-2” was 235.90 µ/g BB. Likewise, glucose level in control was 132.88 mg/ml, while in mutant “AB-0.1.60-3-16-1” was 181.48 mg/ml, and that in “AB-0.1.60-3-3-2” was 287.41 mg/ml. “Alibey” mutants should be selected based on two characters i.e. stem panicle length and seed weight/plant. Correlation analysis between panicle number and all other characters were not significant. Plant height significantly affected the grain weight/panicle and the grain weight/plant. It is expected that some of the mutants are adaptable to the tropical lowlands, so that the diversity of wheat germplasm in Indonesia is increased.
INDUKSI AKAR TUNAS KE LAPA SAWIT (Elaeis guineensis Jacq.) SECARA IN VITRO DAN EX VITRO / Root Induction of Oil Palm (Elaeis guineensis Jacq.) Using In Vitro and Ex Vitro Techniques Yunita, Rossa; MARISKA, IKA; PURNAMANINGSIH, RAGAPADMI; LESTARI, ENDANG GATI; UTAMI, SRI
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.37-42

Abstract

Root induction is an important step in the process of propagation of plants through tissue culture techniques. For root induction commonly used plant growth regulator auxin, which has an important role in plant growth and development, especially at the stage of root initiation. The purpose of this study is to obtain appropriate methods for root induction of oil palm in vitro and ex vitro. The experiment used completely randomized factorial design, consisted of three main activities: (1) Induction of rooting on solid media, using combinations of NAA (2 and 4 mg/l) and IBA (1, 2, 3 and 4 mg/l) concentrations with 5 replications; (2) Induction of rooting the liquid media, using three concentrations of NAA (0, 3 and 6 mg/l), each treatment was replicated 5 times; (3) Induction of rooting ex vitro, using rootone F or IBA (20, 40 and 60 mg/l) with 5 replications. Results indicated that the best medium for in vitro root induction on solid media was MS + NAA 4 mg/l IBA + 4 mg/l, while for liquid media was MS + NAA 6 mg/l. Ex vitro rooting induction showed 60% success of acclimatization by soaking vitro shoots base in NAA solution 40 mg/l and 60 mg/l for 1 hour.Keywords: Elaeis guineensis Jacq., ex vitro, IBA, NAA
Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and G ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI; MUHAMAD SYUKUR; RAGAPADMI PURNAMANINGSIH; ROSSA YUNITA; ROHIM FIRDAUS
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.16

Abstract

Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and Gunung Putri (1540 m asl). Genotype 8 had the latest age of flowering in the three locations than the other genotypes, however, the growth and biomass were the lowest. Vegetative growth of Artemisia in Pacet and Gunung Putri was better than those in Cicurug. Genotype of 15 in Cicurug and 5A genotype in Gunung Putri and Pacet had higher wet and dry weight than that of two other associates. Based on plant biomass, 5 genotypes from Gunung Putri and Pacet i.e. 1D, 3, 5A, 14, and 15 genotypes were selected, as well as 5 genotypes i.e. 1D, 3, 4, 5A, and 15 genotypes from Cicurug. Analisys on artemisin content successfully obtained 5 selected somaclone lines i.e. 1B, 2, 4, 14, and 3 somaclones.
Genetic Variation of the First Generation of Rodent Tuber (Typhonium flagelliforme Lodd.) Mutants Based on RAPD Molecular Markers NESTI FRONIKA SIANIPAR; DANNY LAURENT; RAGAPADMI PURNAMANINGSIH; IRENG DARWATI
HAYATI Journal of Biosciences Vol. 22 No. 2 (2015): April 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1930.619 KB) | DOI: 10.4308/hjb.22.2.98

Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) is a herbal plant from the Araceae family. The plant has high medical potential and is effective to cure cancer. However, the low level of its genetic variation limits its exploration for desirable traits. The low level of genetic variation in Rodent tuber is mainly due to its asexual reproduction system. It usually reproduces vegetatively via tuber separation. Therefore, gamma irradiation had been applied to rodent tuber in vitro to increase its genetic diversity. The objective of this study was to analyze the genetic diversity of the first generation (MV1) of gamma irradiated rodent tuber mutant using random amplified polymorphic DNA (RAPD) markers. A total of 14 mutant DNA samples were analyzed with 14 RAPD primers. The result showed that 67 out of 123 DNA bands were polymorphic among mutant lines. Based on cluster analysis these mutants showed 0.78-0.97 genetic similarity. Cutting of dendogram at genetic distance of 0.89 produced four main clusters. Mutants with high genetic variation are now available. This increases the opportunity of obtaining mutant lines with high anti-cancer activity.
Increasing Al-Tolerance of Sugarcane Using Ethyl Methane Sulphonate and In Vitro Selection in the Low pH Media Ragapadmi Purnamaningsih; Sri Hutami
HAYATI Journal of Biosciences Vol. 23 No. 1 (2016): January 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (799.665 KB) | DOI: 10.4308/hjb.23.1.1

Abstract

Increased production of sugarcane in Indonesia can be done with extensification sugarcane plantations which largely dominated by acidic upland red-yellow podzolic soil. High aluminium (Al) content and low pH of the soil can inhibit plant growth and development. Tolerant sugarcane in acid soil is the most efficient way, but the adaptive variety is still limited. In vitro culture technique can increase genetic variability to assemble new varieties through somaclonal variation combined with mutation using ethyl methane sulphonate (EMS). The new characters was directed by in vitro selection using AlCl3.6H2O with pH = 4 as a component of selection for resistance to high aluminium. VMC 7616 and PS 862 varieties were used as materials. Mutation induced using EMS at concentrations of 0.1%, 0.3%, and 0.5% for 30, 60 and 120 minutes. Plantlets mutant obtained through callus formation, immersion callus in EMS, in vitro selection, and regeneration of callus. Result of study showed that the long immersion in the EMS solution caused greater damage to the cells, as indicated by the change in callus colour. Callus immersion time in EMS gave greater influence to regeneration compared to concentration of EMS. PS 862 had higher Al tolerance than VMC 7616. Rooting of shoot induced using indole-3-butyric acid (IBA) 3 mg/L.
INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (Triticum aestivum L.) Laela Sari; Agus Purwito; Didy Soepandi; Ragapadmi Purnamaningsih; Enny Sudarmonowati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.087 KB) | DOI: 10.29122/jbbi.v3i2.36

Abstract

INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivum L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27–35°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27–35°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus
REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS I. Roostika; R. Purnamaningsih; I. Darwati; I. Mariska
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v8n2.2007.p60-66

Abstract

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangered plant which has various medicinal properties such as aphrodisiac, diuretic, and tonic. The plant is commonly harvested from its natural habitat, therefore it becomes endangered. Regeneration of pruatjan through organogenesis has been studied, but its shoot multiplication was very low (5 shoots per explant). The study aimed to investigate the best regeneration technique of pruatjan through somatic embryogenesis. This research was conducted at the tissue culture laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development in 2004-2005. Callus formation of pruatjan was induced from the petioles and leaves in Driver and Kuniyaki’s (DKW) based medium containing 2,4-D combined with picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogenic calli were then transferred into embryo development medium in two ways. First, they were directly transferred into media containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm. Second, they were indirectly transferred into media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/ NAA media. Parameters evaluated were fresh weight, dry weight, time initiation of embryogenic callus formation, and total number of embryos. The result showed that calli of pruatjan were successfully induced from the petioles and leaves. The best calli were induced from the leaves in the DKW medium containing 2.0 ppm 2,4-D and 0.5 ppm picloram. Embryo development of the calli was best if they were first grown in the media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate then transferred to the IBA/NAA media. The total number of somatic embryos was counted up to 103 on the medium containing 1.5 ppm IBA. This study indicated that pruatjan somatic embryogenesis regeneration required three different media, i.e. for callus induction, development and maturation, and for germination.
Regenerasi Tanaman Sedap Malam Melalui Organogenesis dan Embriogenesis Somatik Ika Rostika; Ika Mariska; R Purnamaningsih
Jurnal Hortikultura Vol 15, No 4 (2005): Desember 2005
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v15n4.2005.p%p

Abstract

Secara konvensional perbanyakan tanaman sedap malam dilakukan melalui umbi. Semakin kecil ukuran umbi semakin lama tanaman berbunga. Penerapan teknik kultur in vitro diharapkan dapat membantu perbanyakan tanaman secara masal. Hingga saat ini, teknik kultur in vitro tanaman sedap malam belum pernah dilaporkan di Indonesia. Penelitian ini bertujuan memperoleh formulasi media yang efektif menginduksi organogenesis dan embriogenesis kultur in vitro tanaman sedap malam serta memacu regenerasinya. Percobaan dibagi menjadi 4 tahap, yaitu (1) induksi tunas, (2) multiplikasi tunas, (3) induksi kalus embriogenik, dan (4) regenerasi kalus embriogenik. Media induksi tunas yang diuji adalah MS+BA 0 ppm, MS+BA 3 ppm, MS+BA 5 ppm, dan MS+BA 7 ppm. Pemacuan multiplikasi tunas lanjut dilakukan pada media subkultur MS+BA 7 ppm+glutamin 100 ppm, MS+BA 7 ppm, DKW+TDZ 7 ppm, dan DKW+TDZ 7 ppm+glutamin 100 ppm. Untuk induksi kalus embriogenik, media induksi kalus yang diujikan adalah MS+2,4-D 2,5 ppm, MS +2,4-D 5 ppm, dan MS+2,4-D 10 ppm. Untuk meregenerasikan kalus embriogenik, media yang diujikan MS+BA 2 ppm+TDZ 0,2 ppm, MS+BA 3 ppm+TDZ 0,4 ppm, MS+zeatin 1ppm+kinetin 1ppm, dan MS+zeatin 0,5 ppm+kinetin 2 ppm. Hasil percobaan menunjukkan bahwa pembentukan tunas terbanyak diperoleh dari media BA 3 ppm (80%) namun inisiasi tunas tercepat dihasilkan pada media BA 0 ppm. Formula media MS+BA 7 ppm+glutamin 100 ppm menghasilkan jumlah tunas dan akar terbanyak. Penggunaan MS+2,4-D 5 ppm dapat menginduksi kalus embriogenik dengan persentase pembentukan nodul sebesar 18,75% dan jumlah nodul yang terbentuk sebanyak 3,6 dengan visual kalus yang paling baik. Setelah disubkultur, calon tunas terbanyak (17) dihasilkan dari perlakuan MS+BA 2 ppm+TDZ 0,4 ppm. Kalus embriogenik pada media MS+zeatin 0,5 ppm+kinetin 2 ppm dapat berkembang membentuk benih somatik.Regeneration of tuberose through organogenesis and embryogenesis. Tuberose is normally propagated by the tuber. The smaller size of tuber the longer time plant to flower. The application of in vitro culture technique might be used for mass propagation. Up to know, the research of in vitro culture of tuberose in Indonesia has not been reported. The objective of the study was to find out media formulation for organogenesis and embryogenesis. The experiments consisted of 4 steps of (1) shoot induction, (2) shoot multiplication, (3) induction of embryogenic callus, and (4) regeneration of embryogenic callus. The treatments for shoot induction were MS+BA 0 ppm, MS+BA 3 ppm, MS+BA 5 ppm, and MS+BA 7 ppm. The shoots were multiplied on media MS+BA 7ppm+glutamine 100ppm, MS+BA 7 ppm, DKW+TDZ 7 ppm, and DKW+TDZ 7 ppm+glutamin 100 ppm. For induction of embryogenic callus, the treatments were MS+2.4-D 2.5 ppm, MS+2,4-D 5 ppm, and MS+2.4-D 10 ppm. For regeneration of embryogenic callus, the treatments were MS+BA 2 ppm+TDZ 0.2 ppm, MS+BA 3 ppm +TDZ 0.4 ppm, MS+zeatin 1ppm+kinetin 1ppm, and MS+zeatin 0.5 ppm+kinetin 2 ppm. The results showed that the highest shoot formation was obtained from media MS+BA 3 ppm but the earliest shoot initiation was obtained from media MS+BA 0 ppm. The media formulation of MS+BA 7 ppm+glutamine 100 ppm gave the highest number of shoot and root. The application of media MS+2.4-D 5 ppm could induce embryogenic callus with high percentage of nodul formation (18.75%) and high number of nodul (3.6) with the best visual calli. After subculturing, the highest number of nodul (17) was obtained from media MS+BA 2 ppm+TDZ 0.4 ppm. The embryogenic callus from media MS+zeatin 0.5 ppm+kinetin 2 ppm could develop to form somatic seed.
Pembentukan Benih Sintetik Tanaman Nenas I Roostika; R Purnamaningsih; Y Supriati; Ika Mariska; N Khumaida; A G Wattimena
Jurnal Hortikultura Vol 22, No 4 (2012): Desember 2012
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v22n4.2012.p316-326

Abstract

Nenas merupakan tanaman buah tropis dan subtropis yang komersial. Kultivar Smooth Cayenne memiliki tipe dan jumlah propagul yang terbatas, sehingga diperlukan dukungan teknologi lainnya untuk produksi benih secara masal. Teknologi benih sintetik dapat diterapkan untuk produksi benih secara masal dan konservasi. Tujuan  penelitian ialah untuk mengetahui pengaruh kombinasi auksin dan sitokinin terhadap morfogenesis eksplan nenas yang terenkapsulasi, mengetahui pengaruh interaksi antara suhu penyimpanan dengan konsentrasi paklobutrazol atau manitol terhadap pertumbuhan eksplan nenas yang terenkapsulasi dan masa simpan. Penelitian dilaksanakan dari Bulan April sampai dengan Desember 2011 di Laboratorium Kultur Jaringan, Kelompok Peneliti Biologi Sel dan Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor. Percobaan disusun secara faktorial dalam rancangan acak lengkap terdiri atas enkapsulasi eksplan, pertumbuhan minimal menggunakan paklobutrazol, atau manitol yang dikombinasikan dengan suhu penyimpanan. Enkapsulasi dilakukan terhadap batang semu dan basal daun menggunakan Na-alginat 3% yang berisi media MS dengan penambahan BA (0, 1, 2, dan 3 mg/l) yang dikombinasikan dengan NAA (0, 1, 2, dan 3 mg/l). Untuk memacu proses diferensiasi, basal daun diberi praperlakuan menggunakan media MS yang mengandung BA 0,5 mg/l dan NAA 0,5 mg/l sebelum dienkapsulasi dengan perlakuan BA dan NAA pada konsentrasi 0; 0,5; dan 1 mg/l.  Pertumbuhan minimal dilakukan menggunakan paklobutrazol (0, 1, 2, dan 3 mg/l) atau manitol (0, 1, 2, 3, 4, dan 5%) pada suhu penyimpanan 15 dan 25 0C. Hasil penelitian menunjukkan bahwa basal daun nenas yang terenkapsulasi mampu berdiferensiasi setelah praperlakuan. Tidak terdapat interaksi yang nyata antara konsentrasi paklobutrazol dengan suhu penyimpanan terhadap daya hidup dan daya tembus kapsul tunas nenas. Biakan tersebut hanya dapat disimpan selama 1 bulan. Interaksi yang nyata juga tidak dijumpai antara konsentrasi manitol dengan suhu penyimpanan terhadap daya hidup dan daya tembus kapsul embrio somatik nenas. Manitol 4% mampu memperpanjang masa simpan hingga 4 bulan. Manitol dapat menggantikan aplikasi suhu rendah dalam penyimpanan kultur nenas yang terenkapsulasi.
In Vitro Culture Manipulation on Pruatjan for Secondary Metabolite Production Ika Roostika; Ragapadmi Purnamaningsih; Ireng Darwati; Ika Mariska
Jurnal AgroBiogen Vol 3, No 2 (2007): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v3n2.2007.p55-59

Abstract

Purwoceng (Pimpinella pruatjan Molk. atau Pimpinellaalpina KDS.) adalah tanaman obat langka yang dapat dimanfaatkansebagai bahan obat afrodisik, diuretik, dan tonik.Kultur in vitro tidak hanya dapat digunakan untuk konservasidan perbanyakan tanaman, melainkan dapat juga diterapkanuntuk produksi metabolit sekunder. Melalui teknik ini,produksi metabolit sekunder tidak bergantung kepada sumbertanaman di lapang. Penelitian ini dilakukan dengan tujuanuntuk meningkatkan kadar stigmasterol melalui kultur invitro dengan menggunakan prekursor asam mevalonat. Penelitiandibagi menjadi dua tahap, yaitu induksi kalus danmanipulasi kultur in vitro untuk meningkatkan kadar stigmasterol.Pada tahap induksi kalus, terdapat 16 perlakuan yangmerupakan kombinasi perlakuan 2,4-D dan piklorammasing-masing pada taraf 0,5; 1,0; 1,5; dan 2,0 ppm. Untukmeningkatkan kadar stigmasterol, digunakan asam mevalonatpada taraf 0, 250, 500, dan 750 ppm dengan masa inkubasiselama 4 dan 6 minggu. Kandungan stigmasterol dianalisismenggunakan GC-MS. Hasil penelitian menunjukkanbahwa media P2 (DKW + 2,4-D 0,5 ppm + pikloram 1,0ppm) adalah media terbaik untuk induksi kalus. Eksplan daunlebih baik daripada eksplan petiol. Hasil analisis GC-MSmenunjukkan bahwa kandungan stigmasterol tertinggi(0,0356 ppm) diperoleh dari kalus dengan masa inkubasi 4minggu pada media dengan penambahan asam mevalonat250 ppm. Peningkatan taraf asam mevalonat tidak mampumeningkatkan kandungan stigmasterol. Kadar tersebut miripdengan kandungan stigmasterol pada planlet dari GunungPutri (0,0365 ppm) dan Dieng (0,0414 ppm). Dibandingkandengan kadarnya dalam akar tanaman dari lapang, kandungantersebut sekitar 10-100 kali lipat lebih tinggi.
Co-Authors A G Wattimena Agus Purwito Ashrina, Misky Azrai, Muh. DANNY LAURENT Darliah Darliah Deden Sukmadjaja Diantina, Surya Didy Soepandi Didy Soepandi, Didy Didy Sopandie E.G. Lestari E.G. Lestari E.G. Lestari Endang G Lestari Endang G Lestari Endang Gati Lestari Endang Gati Lestari ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI Enny Sudarmonowati Enny Sudarmonowati GA Wattimena Hutami, Sri I Mariska I Roostika I. Darwati I. Darwati I. DARWATI I. Mariska I. Roostika I. Roostika I. ROOSTIKA Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Rostika Ika Rostika IRENG DARWATI Ireng Darwati Laela Sari laela Sari, laela Lestari, Endang Gati LESTARI, ENDANG GATI Lizawati . Mariska, I Mariska, Ika MARISKA, IKA Muhamad Syukur Muhammad Syukur N Khumaida N Khumaida NESTI FRONIKA SIANIPAR Nesti Fronika Sianipar NESTI FRONIKA SIANIPAR Noviati, Arief V. Nur, Amin Nurhayani, Siti Pardal, S.J. Pardal, Saptowo Jumali Rita Megia RITA MEGIA ROHIM FIRDAUS ROHIM FIRDAUS Rohim Firdaus Roostika, Ika Rosa Yunita Rosa Yunita Rosa Yunita Rosaria Rosiana Rosaria Rosiana, Rosaria ROSSA YUNITA Rossa Yunita S.J. Pardal Sari, Laela Siti Nurhayani Slamet . Slamet Slamet Slamet, nFN Soeranto, Soeranto Sri Hutami Sri Hutami Sri Hutami Subagio, Herman Sudarmonowati, Enny Sustiprajitno, Sustiprajitno Syarifah Iis Aisyah Tri P. Priyatno Trias Novita Trikoesoemaningtyas UTAMI, SRI Wahyu Handayati Wahyu Handayati Y Supriati Y Supriati Yati Supriati, Yati Yunita, Rossa