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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmiah Aplikasi Isotop Radiasi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Penelitian Tanaman Industri (Littri)
RAGAPADMI PURNAMANINGSIH
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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Keragaman Somaklonal untuk Perbaikan Tanaman Artemisia (Artemisia annua L.) melalui Kultur In Vitro Endang G Lestari; Ragapadmi Purnamaningsih; Muhammad Syukur; Rosa Yunita
Jurnal AgroBiogen Vol 6, No 1 (2010): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n1.2010.p26-32

Abstract

Somaclonal Variability for the Improvement of PlantsArtemisia (Artemisia annua L.) by In Vitro Culture.Endang G. Lestari, Rosa Yunita, and Ali Husni. Artemisiaannua L., a family member of Asteraceae, is medicinalplants originated from China. The plant has been widelyused by the local people for malaria remedy. Its active substance,artemisine, has been proved to hamper the malariabacteria incubation, Plasmodium sp. In accordance with theWHO recomendation, the Department of Health of Indonesiais now in the attempt of developing this plant as thesubtitute of chloroquin because of the malaria bacteriaresistance to this antidote. In Indonesia, the artemisinecontent of the plant less than 0,5% is the crucial problemleading no investors are interested in its economic value.Therefore, Indonesian Medicinal and Spice Crops ResearchInstitute; BPTO Tawangmangu, Indonesian Institute ofSciences; and PT Kimia Farma cooperate for obtaining theprime clone by breeding, selection, as well as environmentaladaptation. In coping with the problem, ICABIOGRAD in thecollaboration with Bogor Agricultural University haveconducted the research for genetic improvement throughmutative induction and field selection. This research onsomaclonal variation. was conducted from Januari 2006 toJuni 2008. Eksplan used for experiment were shoots radiatedwith 10-100 Gy gamma ray. The result showed that the shootradiated with the dosage of 70-100 Gy was unable to grow.On the other hand, the high level of multiplication wasacquired in the one radiated with 10-30 Gy. The optimumradiation for somaclonal radiation was eventually gainedwith 40-60 Gy. The somaclone lines with 10-60 Gy radiationhave been aclimatized and planted in Gunung Putri plot inthe elevation of 1545 asl. Artemisinin content at the highbiomases genotype is 0,49-0,52%.
Seleksi In Vitro dan Pengujian Mutan Tanaman Pisang Ambon Kuning untuk Ketahanan terhadap Penyakit Layu Fusarium Deden Sukmadjaja; Ragapadmi Purnamaningsih; Tri P. Priyatno
Jurnal AgroBiogen Vol 9, No 2 (2013): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n2.2013.p66-76

Abstract

Fusarium wilt of banana (Musa spp.) caused byFusarium oxysporum f. sp. cubense (Foc) is the most seriousproblem faced in banana cultivation in terms of plantproductivity and fruit quality. Mutation breeding is one of thealternative method that can be applied in producing newbanana cultivar. Mutants can be induced by chemicalmutagen such as ethyl methane sulfonate (EMS) followed byin vitro selection and then evaluation of the mutants tofusarium wilt disease in glasshouse and Foc infected field.The aim of this research was obtained EMS induced and invitro selected mutants of banana var. Ambon Kuning andevaluated Foc disease resistant clones in glasshouse andFoc infected field. The first step to obtain the explants forthis research was initiation and formation of multiple budclumps (MBC) using MS basal media supplemented with 5,10, and 20 mg/l of benzyladenin. Plant regeneration of MBCwas also studied by using MS media containing 0, 0.2, and 1mg/l of benzyladenin. To induce mutagenesis, MBC wassoaked in 0.1, 0.3, and 0.5% (v/v) EMS for 1, 2, and 3 hours.The assesment of resistant MBC mutants to Fusariumphytotoxin was conducted by using fusaric acid (FA) asselection agent in concentration of 30, 45, and 60 ppm.Putative mutant plants produced by in vitro selection werefurther tested using spore solution of Foc race 4 inglasshouse. Meanwhile, Foc resistance assesment in theinfected field was conducted in Pasirkuda ExperimentalStation, Bogor Agricultural University. The results showedthat MBC can be formed in MS basal media supplementedwith 10 or 20 mg/l benzyladenin. The EMS played a role inobtaining mutants by producing 68 MBC putative mutantstolerant to Foc based on FA selection. Further evaluation inthe glasshouse was obtained 64 Foc resistant plants from391 putative mutants produced by in vitro selection.Evaluation in the Foc infected field showed six clonessurvived until generative phase (12 month of age).
PENYIMPANAN IN VITRO TANAMAN PURWOCENG (Pimpinella pruatjan Molk.) MELALUI APLIKASI PENGENCERAN MEDIA DAN PACLOBUTRAZOL I. ROOSTIKA; R. PURNAMANINGSIH; I. DARWATI
Jurnal Penelitian Tanaman Industri Vol 15, No 2 (2009): Juni 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n2.2009.84-90

Abstract

ABSTRAKPurwoceng (Pimpinella pruatjan Molk.) adalah tanaman obatlangka asli Indonesia yang dikategorikan hampir punah. Konservasi in situtidak dapat diandalkan karena rusaknya habitat alami (hutan konservasi),sedangkan konservasi ex situ di lapang menghadapi kendala karenapurwoceng sulit dibudidayakan di luar habitat aslinya. Dengan demikian,konservasi in vitro merupakan alternatif yang dapat diterapkan untukmenghindari kepunahan tanaman purwoceng. Tujuan penelitian untukmengetahui efek dari kombinasi perlakuan pengenceran media dankonsentrasi paclobutrazol terhadap pertumbuhan kultur purwoceng, dayaregenerasi dan stabilitas genetik pasca penyimpanan. Penelitian dilakukanpada tahun 2004 di Laboratorium Kultur Jaringan, Balai Penelitian danPengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogorselama 9 bulan. Bahan tanaman yang digunakan bersumber dari koleksitanaman purwoceng di Kebun Percobaan Gunung Putri, Balai PenelitianTanaman Rempah dan Obat. Kegiatan penelitian mencakup: (1)Perbanyakan tunas in vitro purwoceng sebagai sumber eksplan denganmenggunakan regenerasi, yaitu media DKW + BA 1 ppm + Thidiazuron0,2 ppm + arginin 100 ppm, (2) Penyimpanan in vitro tunas purwocengdalam media DKW (1, ½, dan ¼ dosis) + paclobutrazol (0, 1, 3, dan 5ppm), (3) Regenerasi kultur purwoceng pasca penyimpanan in vitro padamedia regenerasi, dan (4) Evaluasi karakter sitologi kultur yang telahdisimpan melalui penghitungan jumlah kloroplas sel penjaga stomata.Rancangan percobaan disusun secara faktorial dalam lingkungan acaklengkap dengan 6 ulangan. Hasil penelitian menunjukkan tidak adanyainteraksi yang nyata antara pengenceran media dan konsentrasipaclobutrazol. Periode simpan kultur tidak dapat diperpanjang lebih dari 4bulan karena paclobutrazol mempunyai pengaruh penghambatan pertum-buhan yang sangat kuat sehingga sebagian besar kultur purwoceng mati.Efek residu paclobutrazol masih tampak pada jangka waktu lebih dari 4bulan pada tahap pemulihan, ditandai dengan adanya penampilan roset.Pengamatan ciri sitologi melalui penghitungan jumlah kloroplas selpenjaga stomata menunjukkan bahwa penggunaan paclobutrazol tidakmenyebabkan perubahan tingkat ploidi. Disimpulkan bahwa paclobutrazoltidak sesuai digunakan untuk penyimpanan in vitro purwoceng karenamenyebabkan pertumbuhan yang abnormal (roset) sekalipun pada tahapregenerasi pasca penyimpanan. Selanjutnya disarankan untuk mengguna-kan regulator osmotik, yang mampu meningkatkan potensi osmotik dalammedia dan memperlambat penyerapan nutrisi sehingga masa simpankemungkinan dapat diperpanjang tanpa menyebabkan pertumbuhan yangabnormal pada tahap regenerasi pasca penyimpanan.Kata kunci : Pimpinella pruatjan Molk., penyimpanan in vitro, pengen-ceran media, dan paclobutrazolABSTRACTPurwoceng (Pimpinella pruatjan Molk.) is an Indonesian medicinalplant categorized as endangered plant. In situ conservation is quiteimpossible since conservation forest has been damaged whereas ex situconservation in the field is difficult because the plant needs specificagronomical condition. In vitro conservation is therefore the only choice tobe applied. The objectives of the study were to find out the effects ofcombined  treatment  between  media  dilution  and  paclobutrazolconcentration to the growth of pruatjan cultures, the genetic regenerationand stability after preservation. The research was conducted at the TissueCulture Laboratory, the Indonesian Center for Agricultural Biotechnologyand Genetic Resources Research and Development for 9 months. The plantmaterials were taken from Gunung Putri. The activities included: (1)Propagation of in vitro shoots as explants source in DKW media + 1 ppmBA + 0.2 ppm Thidiazuron + 100 ppm arginin, (2) Preservation of in vitroshoots of pruatjan on DKW (full, half, and quarter strength) +paclobutrazol (0, 1, 3, and 5 ppm), (3) Regeneration of the cultures after invitro preservation, and (4) Evaluation of cytological character of preservedcultures through chloroplast guard cells counting. The experiment wasarranged factorially in Completely Randomized Design with 6replications. The result revealed that there was no interaction betweenmedia dilution and paclobutrazol concentration. Preservation period couldnot be prolonged more than 4 months because this compound stronglyinhibited the growth so that almost none of them could survive longer. Theresidual effect of paclobutrazol was still appeared more than 4 months inregeneration phase assigned by rossette performances. Observation ofcytological character through chloroplast guard cells counting revealedthat paclobutrazol could not change ploidy level of preserved pruatjancultures. It was concluded that paclobutrazol is not suitable for in vitropreservation of pruatjan since it causes abnormal growth on regenerationstep after preservation. Thus, it was suggested to use osmotic regulatorwhich can increase osmoticum potential in media and decrease nutritionabsorption so that preservation period may be prolonged without abnormaleffect on regeneration step after preservation.Key words: Pimpinella pruatjan Molk., in vitro preservation, mediadilution, and paclobutrazol
Pengaruh Iradiasi Gamma dan Ethyl Methan Sulfonate Terhadap Pembentukan Embriosomatik Kedelai (Glycine max L.) Ragapadmi Purnamaningsih; Ika Mariska; E.G. Lestari; Sri Hutami; Rossa Yunita
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 10, No 1 (2014): Juni 2014
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2014.10.1.2746

Abstract

Kedelai merupakan salah satu sumber protein dan lemak nabati yang penting. Perubahan iklim global berpengaruh terhadap produktivitas kedelai, sehingga diperlukan kultivar-kultivar baru yang mempunyai sifat unggul tertentu agar produktivitas kedelai dapat ditingkatkan. Teknik in vitro dengan mutasi dan keragaman somaklonal merupakan meoda alternatif untuk memperoleh varietas baru apabila material genetik sebagai bahan seleksi tidak tersedia. Induksi mutasi dapat dilakukan pada populasi sel embriogenik dengan menggunakan iradiasi sinar gamma atau senyawa kimia, antara lain Ethyl Methan Sulfonate (EMS). Kedua metoda tersebut telah banyak digunakan untuk meningkatkan keragaman genetik tanaman dan telah dihasilkan galur-galur baru dengan sifat unggul. Salah satu masalah penting yang harus dikuasai dalam penerapan teknologi tersebut adalah meregenerasikan sel somatik hasil mutasi dan keragaman somaklonal agar dapat ditumbuhkan menjadi planlet (tunas in vitro). Beberapa faktor yang mempengaruhi regenerasi tanaman adalah jenis bahan tanaman, genotipe, komposisi media, dll. Perlakuan keragaman somaklonal dan mutasi yang diberikan dapat menyebabkan kerusakan pada sel sehingga diperlukan modifikasi pada metoda regenerasi yang sudah diketahui agar populasi sel yang hidup setelah perlakuan mutasi dapat tumbuh menjadi tunas-tunas mutan. Tujuan penelitian adalah untuk mendapatkan planlet mutan hasil perlakuan mutasi dengan iradiasi gamma dan EMS. Varietas kedelai yang digunakan adalah Wilis, Burangrang, Baluran dan aksesi No. B 3592. Eksplan yang digunakan adalah embriozigotik muda berasal dari polong yang berumur 12-20 hari setelah penyerbukan. Induksi kalus embriogenik dilakukan dengan menggunakan media MS + vitamin Gamborg (B5) dengan penambahan 2,4-D 20 mg/l dan sukrosa 3%. Kalus yang didapatkan diberi perlakuan mutasi menggunakan sinar gamma pada dosis 400 rad atau direndam dalam larutan EMS (0.1 %, 0.3 %, dan 0.5 %) selama 1, 2 dan 3 jam. Selanjutnya kalus dipindahkan pada media untuk menginduksi pembentukan benih somatik. Hasil penelitian menunjukkan bahwa pembentukan kalus dipengaruhi oleh genotipe tanaman. Pembentukan kalus tertinggi dihasilkan dari Baluran (93.40%) dan terendah Burangrang (75.90%). Perlakuan iradiasi gamma menurunkan pembentukan struktur torpedo, dimana struktur torpedo tertinggi diperoleh dari Burangrang (25.4-26.3/eksplan). Aksesi B 3592 mempunyai kemampuan membentuk struktur torpedo paling tinggi pada semua perlakuan EMS yang digunakan. Perendaman kalus dalam larutan EMS 0.5% selama 1, 2, dan 3 jam menurunkan regenerasinya membentuk struktur torpedo pada semua genotipe. Perlakuan EMS menyebabkan kerusakan sel yang lebih besar dibandingkan dengan iradiasi sinar gamma, ditunjukkan dengan persentase pembentukan struktur torpedo setelah perlakuan EMS (0-15/eksplan) lebih kecil dibanding dengan iradiasi sinar gamma (10.3-26.3/eksplan). Kata kunci :   Glycine max, iradiasi sinar gamma, Ethyl Methan Sulfonate, embriogenesis somatik
Transformasi Genetik Pisang Ambon dengan Gen Kitinase dari Padi Ragapadmi Purnamaningsih; Deden Sukmadjaja
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p97-104

Abstract

One of the main constrains on theproductivity and quality enhancement of banana is wiltdiseases caused by Fusarium oxysporum (Foc). Productiondecrease by wilt disease was 63.33%. Therefore, an effort toobtain the banana new variety which is tolerant to fusariumwas absolutely necessary to be done. Genetic engineeringcan be used in new variety improvement, especially forproduction of pest and disease tolerant varieties.Transformation of banana with chi gene which expressedchitinase enzyme have been used in obtaining the plantresistant to Foc. The goals of the research were to obtain:determine lowest higromisin consentration inhibited nodulgrowth by tested four consentration of higromisin,determine optimum cocultivation time by tested three timescocultivation, tested asetosiringone added on two timescocultivation, and gen chi introduction at bananatransforman shoots with PCR. The explants used werenodule induced from pseudostem of banana cv. Ambonkuning. Genetic transformation done by sowing the explantsin bacterial suspension 0, 15, 30, and 45 minutes.The effectof asetosiringone (0 and 100 mg/l) on cocultivation mediumwas observed. The research results showed that the lowesthigromisin concentration inhibited nodule growth was 25mg/l for 5 weeks and the best time for inoculation of nodulewere 30 minute. Asetosiringone added on bacterialsuspension did not increase transformation efficiency.Chitinase gene transformation using Agrobacteriumtumefaciens on banana nodules produced 25 noduly ines ofputative transformant on selection media and 34 plantstransforman identification by PCR.
PENINGKATAN KERAGAMAN GENETIK MAWAR MINI MELALUI KULTURIN-VITRO DAN IRADIASI SINAR GAMMA Wahyu Handayati; Darliah Darliah; I Mariska; R Purnamaningsih
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1120

Abstract

Mini rose (Rosa hybrida L.)is one of the favorite ornamental plants. To get a new appearance of this mini rose, two experiments were conducted at Cipanas and Bogor, from April 1999 to March 2000.In the first experiment, the treatment was the dosage of gamma ray irradiation, i.e. 0, 1, 2, 3, 4, 6, 8, 10 and 12 krad.In the second experiment, the treatment was the concentration of callus induced mediumi.e. 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5 and 1 mg/1) + chinetine (1, 3 and 5 mg/1).Randomized Block Design was used with 5 replications. Mini rose Romantica Meilandina (pink color) and Prince Meilandina (dark red color) was used as a source of plant material.The results showed that the irradiation dosage from 1 until 3 krad gave the best appearance compared with the other treatments.Considering the observance to plantlet in the same dosage, the color of the flower was changed from the natural color to white and red color.The combination of 0,5 mg/12,4- dichlorophenoxyacetic acid + 3 mg/1 chinetine was the best medium to the callus growth.
POLA INSERSI PARTENOKARPI, DefH9-iaaM PADA GALUR TOMAT TRANSGENIK S.J. Pardal; Slamet Slamet; R. Purnamaningsih; E.G. Lestari
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.691

Abstract

The development of seedless tomato fruits will be more attractive to consumers and industry. Artificial parthenocarpy can be induced through genetic crossing, hormone application or genetic engineering. Development of parthenocarpic tomatos has been done by inserting parthenocarpy gene, DefH9-iaaM into tomato genome via Agrobacterium tumefaciens. Sixty putative transgenic tomato lines were produced, and three events (lines) have been selected as the best event, i.e. OvR1#14-4, OvM2#10-1, OvM2#6-2. These lines contained the DefH9-iaaM based on PCR test. This research aimed was to determine the insertion patern of DefH9-iaaM gene in the progeny of transgenic tomatos lines. Parent variety Oval and line Cl 6046 were used as control plants. Results indicated that tomatos line OvR1#14-4 was still contained the inserted DefH9-iaaM gene and followed the Mendelian pattern (3:1) based on molecular analyses and Chi-square test results, while the others were not identified. Line OvR1#14-4 was required to be further evaluated for phenotypic and genotypic analyses for the expression of their parthenocarpy.
PENYIMPANAN IN VITRO TANAMAN PURWOCENG (Pimpinella pruatjan Molk.) MELALUI APLIKASI PENGENCERAN MEDIA DAN PACLOBUTRAZOL I. ROOSTIKA; R. PURNAMANINGSIH; I. DARWATI
Jurnal Penelitian Tanaman Industri Vol 15, No 2 (2009): Juni 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n2.2009.84-90

Abstract

ABSTRAKPurwoceng (Pimpinella pruatjan Molk.) adalah tanaman obatlangka asli Indonesia yang dikategorikan hampir punah. Konservasi in situtidak dapat diandalkan karena rusaknya habitat alami (hutan konservasi),sedangkan konservasi ex situ di lapang menghadapi kendala karenapurwoceng sulit dibudidayakan di luar habitat aslinya. Dengan demikian,konservasi in vitro merupakan alternatif yang dapat diterapkan untukmenghindari kepunahan tanaman purwoceng. Tujuan penelitian untukmengetahui efek dari kombinasi perlakuan pengenceran media dankonsentrasi paclobutrazol terhadap pertumbuhan kultur purwoceng, dayaregenerasi dan stabilitas genetik pasca penyimpanan. Penelitian dilakukanpada tahun 2004 di Laboratorium Kultur Jaringan, Balai Penelitian danPengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Bogorselama 9 bulan. Bahan tanaman yang digunakan bersumber dari koleksitanaman purwoceng di Kebun Percobaan Gunung Putri, Balai PenelitianTanaman Rempah dan Obat. Kegiatan penelitian mencakup: (1)Perbanyakan tunas in vitro purwoceng sebagai sumber eksplan denganmenggunakan regenerasi, yaitu media DKW + BA 1 ppm + Thidiazuron0,2 ppm + arginin 100 ppm, (2) Penyimpanan in vitro tunas purwocengdalam media DKW (1, ½, dan ¼ dosis) + paclobutrazol (0, 1, 3, dan 5ppm), (3) Regenerasi kultur purwoceng pasca penyimpanan in vitro padamedia regenerasi, dan (4) Evaluasi karakter sitologi kultur yang telahdisimpan melalui penghitungan jumlah kloroplas sel penjaga stomata.Rancangan percobaan disusun secara faktorial dalam lingkungan acaklengkap dengan 6 ulangan. Hasil penelitian menunjukkan tidak adanyainteraksi yang nyata antara pengenceran media dan konsentrasipaclobutrazol. Periode simpan kultur tidak dapat diperpanjang lebih dari 4bulan karena paclobutrazol mempunyai pengaruh penghambatan pertum-buhan yang sangat kuat sehingga sebagian besar kultur purwoceng mati.Efek residu paclobutrazol masih tampak pada jangka waktu lebih dari 4bulan pada tahap pemulihan, ditandai dengan adanya penampilan roset.Pengamatan ciri sitologi melalui penghitungan jumlah kloroplas selpenjaga stomata menunjukkan bahwa penggunaan paclobutrazol tidakmenyebabkan perubahan tingkat ploidi. Disimpulkan bahwa paclobutrazoltidak sesuai digunakan untuk penyimpanan in vitro purwoceng karenamenyebabkan pertumbuhan yang abnormal (roset) sekalipun pada tahapregenerasi pasca penyimpanan. Selanjutnya disarankan untuk mengguna-kan regulator osmotik, yang mampu meningkatkan potensi osmotik dalammedia dan memperlambat penyerapan nutrisi sehingga masa simpankemungkinan dapat diperpanjang tanpa menyebabkan pertumbuhan yangabnormal pada tahap regenerasi pasca penyimpanan.Kata kunci : Pimpinella pruatjan Molk., penyimpanan in vitro, pengen-ceran media, dan paclobutrazolABSTRACTPurwoceng (Pimpinella pruatjan Molk.) is an Indonesian medicinalplant categorized as endangered plant. In situ conservation is quiteimpossible since conservation forest has been damaged whereas ex situconservation in the field is difficult because the plant needs specificagronomical condition. In vitro conservation is therefore the only choice tobe applied. The objectives of the study were to find out the effects ofcombined  treatment  between  media  dilution  and  paclobutrazolconcentration to the growth of pruatjan cultures, the genetic regenerationand stability after preservation. The research was conducted at the TissueCulture Laboratory, the Indonesian Center for Agricultural Biotechnologyand Genetic Resources Research and Development for 9 months. The plantmaterials were taken from Gunung Putri. The activities included: (1)Propagation of in vitro shoots as explants source in DKW media + 1 ppmBA + 0.2 ppm Thidiazuron + 100 ppm arginin, (2) Preservation of in vitroshoots of pruatjan on DKW (full, half, and quarter strength) +paclobutrazol (0, 1, 3, and 5 ppm), (3) Regeneration of the cultures after invitro preservation, and (4) Evaluation of cytological character of preservedcultures through chloroplast guard cells counting. The experiment wasarranged factorially in Completely Randomized Design with 6replications. The result revealed that there was no interaction betweenmedia dilution and paclobutrazol concentration. Preservation period couldnot be prolonged more than 4 months because this compound stronglyinhibited the growth so that almost none of them could survive longer. Theresidual effect of paclobutrazol was still appeared more than 4 months inregeneration phase assigned by rossette performances. Observation ofcytological character through chloroplast guard cells counting revealedthat paclobutrazol could not change ploidy level of preserved pruatjancultures. It was concluded that paclobutrazol is not suitable for in vitropreservation of pruatjan since it causes abnormal growth on regenerationstep after preservation. Thus, it was suggested to use osmotic regulatorwhich can increase osmoticum potential in media and decrease nutritionabsorption so that preservation period may be prolonged without abnormaleffect on regeneration step after preservation.Key words: Pimpinella pruatjan Molk., in vitro preservation, mediadilution, and paclobutrazol
In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood Siti Nurhayani; Rita Megia; Ragapadmi Purnamaningsih
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11079

Abstract

A diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile axillary shoot. The explants were planted on MS0 medium for 2 weeks and later on multiplication medium MS+0.3 mg/l BAP + 0.3mg/l TDZ. The shoots obtained were fragmented into clusters (3-5 shoots) used for the next multiplication stage using five different medium formulas: (1) MS0; MS containing: (2) 0.1 mg/l BAP, (3) 0.3 mg/l BAP, (4) 0.1 mg/l BAP + 0.1 mg/l TDZ and (5) 0.3 mg/l BAP + 0.1 mg/l TDZ. The results showed that MS medium containing 0.1 mg/l BAP + 0.1mg/l TDZ was the best medium for B. balcooa propagation. The shoots produced from aforementioned medium had a better quality compared to the other medium. Forty days after planting, the average number of shoots in this medium was 14.25. MS medium containing 0.3 mg/l BAP + 0.1 mg/l TDZ produced the highest number of shoot but in lower quality. Rooting medium containing 10 mg/l IBA + 5 mg/l NAA produced 9-16 root in 8 weeks. Vermicompost was more prevalent for the acclimatization of B. balcooa compared to compost. The use of B.balcooa resulted in in vitro propagation as a substitute alternative for wood is expected to save the environment from illegal logging.  
Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Syukur, Muhamad; Lestari, Endang Gati; Purnamaningsih, Ragapadmi; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.76

Abstract

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability
Co-Authors A G Wattimena Agus Purwito Ashrina, Misky Azrai, Muh. DANNY LAURENT Darliah Darliah Deden Sukmadjaja Diantina, Surya Didy Soepandi Didy Soepandi, Didy Didy Sopandie E.G. Lestari E.G. Lestari E.G. Lestari Endang G Lestari Endang G Lestari Endang Gati Lestari Endang Gati Lestari ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI Enny Sudarmonowati Enny Sudarmonowati GA Wattimena Hutami, Sri I Mariska I Roostika I. Darwati I. Darwati I. DARWATI I. Mariska I. Roostika I. Roostika I. ROOSTIKA Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Rostika Ika Rostika Ireng Darwati IRENG DARWATI Laela Sari laela Sari, laela LESTARI, ENDANG GATI Lestari, Endang Gati Lizawati . Mariska, I MARISKA, IKA Mariska, Ika Muhamad Syukur Muhammad Syukur N Khumaida N Khumaida Nesti Fronika Sianipar NESTI FRONIKA SIANIPAR NESTI FRONIKA SIANIPAR Noviati, Arief V. Nur, Amin Nurhayani, Siti Pardal, S.J. Pardal, Saptowo Jumali RITA MEGIA Rita Megia Rohim Firdaus ROHIM FIRDAUS ROHIM FIRDAUS Roostika, Ika Rosa Yunita Rosa Yunita Rosa Yunita Rosaria Rosiana Rosaria Rosiana, Rosaria Rossa Yunita ROSSA YUNITA S.J. Pardal Sari, Laela Siti Nurhayani Slamet . Slamet Slamet Slamet, nFN Soeranto, Soeranto Sri Hutami Sri Hutami Sri Hutami Subagio, Herman Sudarmonowati, Enny Sustiprajitno, Sustiprajitno Syarifah Iis Aisyah Tri P. Priyatno Trias Novita Trikoesoemaningtyas UTAMI, SRI Wahyu Handayati Wahyu Handayati Y Supriati Y Supriati Yati Supriati, Yati Yunita, Rossa