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ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN GOLONGAN FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN PRANAJIWA (Euchresta horsfieldii Lesch Benn.) Dewi, Ni Wayan Rika Kumara; Gunawan, I Wayan; Puspawati, Ni Made
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 5 No 1 (2017)
Publisher : Graduate Program of Applied Chemistry, Udayana University, Bali-INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.127 KB) | DOI: 10.24843/CK.2017.v05.i01.p04

Abstract

ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat FE) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3’,4’-pentahidroksi flavonol. ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant activity was carried out using DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. The separation of the ethyl acetate extracts was conducted by column chromatography and the identification by using FTIR and UV-Vis spectrophotometer. The results showed that ethyl acetate extract has antioxidant activity with IC50 value of 393,95 µg/mL and the total flavonoid of 6619,72 mg QE /100g or 6,62% QE. The separation and purification of ethyl acetate extract was chromatography column on silica gel 60 and the solvent n-hexane:chloroform:ethanol (20:1:1) based on UV-Vis and Infrared spectra positive flavonoid isolate (isolates FE) was tentaviely identified as 3,5,7,3',4'-pentadihydroxy flavonols.
Problem-Based Learning Model Using Geogebra Application: Students Motivation to Learn Translation Material of High School Nurfadila, Nurfadila; Hasbi, Muh.; Puspawati, Ni Made
ETDC: Indonesian Journal of Research and Educational Review Vol. 4 No. 3 (2025): June
Publisher : Education and Talent Development Center Indonesia (ETDC Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51574/ijrer.v4i3.3473

Abstract

This study aims to describe the application of the Problem-Based Learning (PBL) model with the help of the GeoGebra application to increase students' learning motivation for geometric transformation material, especially translation. This study is a form of classroom action research carried out in two cycles in class XI E of High School 4 Palu involving 35 students. Each cycle consists of planning, implementation, observation, and reflection steps. In cycle I, the material is related to translation to points, while in cycle II, it is related to translation to lines. Data were collected through a learning motivation questionnaire and observation sheets, which were analyzed descriptively. The results indicated that students' learning motivation increased from cycle I to cycle II. In cycle I, only 6 students (17.1%) were included in the strong motivation category, while 11 students (31.5%) were included in the low category. After the improvement in Cycle II, the number of highly motivated students increased to 15 (42.8%), while those in the low group decreased to only 2 (5.8%). Observational data shows increased student engagement and enjoyment in learning. Consequently, the implementation of the GeoGebra-assisted PBL paradigm has proven successful in increasing motivation in mathematics learning.
Phytochemical and Antioxidant Activity of Lemongrass Stem Extract and Cream Analysis by Fourier Transform Infrared Suaniti*, Ni Made; Riasa, I Nyoman Putu; Puspawati, Ni Made; Samirana, Oka; Wahab, Roswanira Abdul; Valineli, Samanthi Elani; Aryasuadnyana, I Nyoman Krisnanda
Jurnal IPA & Pembelajaran IPA Vol 9, No 3 (2025): SEPTEMBER 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jipi.v9i3.48054

Abstract

Lemongrass is one of the plants that grows in Indonesia, is easy to find, and contains active compounds with potential antioxidant properties. The aim of this study was to analyze phytochemical and antioxidant activity of lemongrass stem extract and functional groups in its cream formulation. The lemongrass stems were extracted using the maceration method with 96% ethanol as the solvent, and the resulting extract was evaporated to obtain a thick lemongrass stem extract. The method of analyze the lemongrass stem extract and the cream formulation was carried out using phytochemical screening and antioxidant activity tests were performed on the lemongrass stem extract against 2,2-diphenyl-1-picrylhydrazyl as a free radical and measured at a wavelength of 517 nm using a UV-Vis spectrophotometer. The cream base, and the cream containing the lemongrass stem extract ware tested by fourier transform infrared spectrophotometer to identify functional groups in the extract and cream was conducted using the KBr pellet method. The results showed that the cream with 10% lemongrass stem extract had the strongest antioxidant activity compared to the 7.5 and 5% concentrations, with an IC50 value of 722.12 ppm, which is categorized as weak antioxidant activity. Functional group analysis showed that the cream without the addition of lemongrass stem extract had no absorption or detectable functional groups, whereas the three creams with lemongrass stem extract showed increased transmittance, with the presumed functional groups being hydroxyl (OH), methyl (CH3), and carbonyl (C=O)
Prevalence and Profile of Adolescents with Sexually Transmitted Infections at the Dermatology and Venereology Polyclinic of Ngoerah Hospital, Bali, Indonesia Intaran, Kadek Devi Aninditha; Puspawati, Ni Made; Elis Indira, I Gusti Ayu Agung; Pemana, Aditya
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 37 No. 3 (2025): DECEMBER
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikkk.V37.3.2025.190-194

Abstract

Background: Sexually Transmitted Infections (STIs) have been considered a public health threat in all countries, both in developed and developing countries. STIs have a direct impact on sexual and reproductive health through stigmatization, infertility, cancer, and pregnancy complications that can increase the risk of HIV. Purpose: This study aims to identify cases of adolescent patients with STIs at Ngoerah Hospital, Denpasar, Bali in the period 2021 to 2023. Methods: This study uses a descriptive observational study with a cross-sectional design. The research was conducted at the Dermatology and Venereology Polyclinic and the Medical Record Installation of Ngoerah Hospital, Denpasar, Bali. After the data was collected, it was tabulated in tabular form and grouped descriptively. Result: A total of 46 patient samples were obtained, with an average age of 16.7 years. Adolescents with STIs were more commonly found in female (63%). Based on their last education, the highest number of patients were junior high school graduates (58.7%) with a history of multipartner sexual intercourse (56.5%). Only 15.2% claimed to be married. The most common type of STIs was syphilis (50%), followed by condyloma acuminata (45.6%). A total of 67.4% of adolescents were known to have HIV-positive status. Conclusion: The picture of STIs in teenagers is more common in female with a junior high school education. Most teenagers have multi-partner partners with a history of having sexual relations with commercial sex workers (CSWs). The most common type of STIs in teenagers is syphilis (50%), and 67.4% are infected with HIV.