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Putu Ayu Sisyawati Putriningsih
Laboratorium Ilmu Penyakit Dalam Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Uji Kepekaan Antibiotika Isolat Escherichia coli O157:H7 asal Feses Ayam I Wayan Suardana; Putu Ayu Sisyawati Putriningsih; Mas Djoko Rudyanto
Buletin Veteriner Udayana Vol. 6 No.1 Pebruari 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.382 KB)

Abstract

Unggas diketahui merupakan salah satu reservoir penting dari agen bakterial zoonosis Escherichia coli O157:H7. Disisi lain, fenomena pemakaian obat obatan pada unggas terutama antibiotika melalui makanan, minuman maupun secara parenteral mengalami peningkatan dari waktu kewaktu. Antibiotika yang digunakan secara intensif ini cenderung tidak sesuai dengan dosis maupun waktu pemakaian sehingga berkontribusi terhadap munculnya peningkatan resistensi termasuk E. coli O157:H7. Penelitian untuk mengetahui  pola kepekaan isolat E. coli O157:H7  ini diawali dengan identifikasi E. coli dengan  penumbuhan  bakteri  asal feses ayam pada media eosin methylene blue agar (EMBA), dilanjutkan dengan uji indol, methyl red, voges-proskauer dan citrat (IMVIC). Identifikasi O157 dilakukan dengan penumbuhan isolat E. coli pada  media selektif  sorbitol MacConkey agar (SMAC),  dilanjutkan dengan uji konfirmasi menggunakan lateks O157. Uji pola kepekaan dilakukan mengacu pada metode Kirby Bauer  seperti yang direkomendasikan oleh national committee for clinical laboratory standard (NCCLS). Hasil penelitian dari 7 isolat  E. coli O157:H7 hasil isolasi 82 sampel feses ayam serta  satu isolat  kontrol ATCC 43894 menunjukan  85,7%  isolat  bersifat resisten terhadap antibiotika metisilin, 71,4% resisten terhadap antibiotika penisilin G, serta 42,9% resisten terhadap antibiotika doksisiklin hidroklorida dan streptomisin. Hasil kajian juga menemukan 42,9% isolat bersifat resisten  terhadap 2 jenis antibiotika,  14,3% resisten  terhadap 3 jenis antibiotika,  14,3%  resisten  terhadap 4 jenis antibiotika  dan 14,3% resisten terhadap 5 jenis antibiotika.  Berdasarkan hasil penelitian ini maka dapat disimpulkan telah terjadinya peningkatan pola resistensi dan bersifat multi-drug resistance dari agen E. coli O157:H7 sehingga perlu dipertimbangkan jenis-jenis antibiotika tertentu yang masih sensitif didalam penanganan infeksi oleh agen ini.
Laporan Kasus: Ankilostomiosis pada Kucing Lokal Mix Persia I Wayan Syartama Hadi Nugraha; Putu Ayu Sisyawati Putriningsih; I Wayan Batan
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.202 KB) | DOI: 10.24843/bulvet.2022.v14.i02.p04

Abstract

Ancylostoma sp. is a common parasite that infects cats and is a major cause of ankylostomyosis. Ancylostoma sp. enter the nematoda class. Cats can be infected through foods containing eggs or infective larvae. A persian mix local cat named Roger, about 7 years old, weighs 3.5 kg, has diarrhea 3 to 4 times a day with blackish brown feces. Based on the results of a physical examination, the cat has diarrhea with a reddish anal mucosa, swelling in the mandibulary lymphogland dexter, eye mucosa, pale nose and mouth and dehydrated cats. The laboratory tests carried out were faecal examination with floating method, McMaster and routine hematology. Roger's cat was diagnosed with mild degrees of ancylostomiosis. Therapy given is pyrantel pamoat with a recommended dose of 6 to 25 mg/kg and the dose given is 20 mg/kg (0.5 tab). Kaolin pectin with recommended doses of 1 to 2 mL/kg (4.5 mL/day). Hematodine with a recommended dose of 0.5 to 2 mL/kg (injected 0.05 mL) intramuscular. Livron Bplex 1 tablet orally once a day for 10 days. Evaluation of cat cases 3 weeks post therapy found that cat cases had no diarrhea, mucosa of the mouth, eyes, ears, vagina, pink sphincter, Capillary Refil Time (CRT) of less than 2 seconds and good skin turgor. Stool examination results with negative native methods were not found for worm egg infestations.
Gambaran Sel Darah Putih Sapi Bali yang Terinfeksi Jamur Dermatofita Secara Alami Nirae Nirae Nurani; Putu Ayu Sisyawati Putriningsih; I Putu Gede Yudhi Arjentinia
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Dermatophytosis is kind of disease which caused by dermatophyta fungus. White blood cell (leukocyte) will responds every strange things which entering the body as a defend cell. In Indonesia, only few information of the white blood cell (leukocyte) in the Dermatophytosis cases towards balinese cattle can be found. The purpose of the research in finding the comparasion between lukocyte of normal balinese cattle which is not infected and balinese cattle which infected by dematophyta fungus. The research are using 12 samples of blood, is abaout 6 blood’s sampel from normal balinese cattle and 6 blood’s sample of balinese cattle which is infected by dermatophyta fungus. The first attempt is checking the skin scratching and the hair with 10% of KOH liquid. Sabouraud’s Dextrose Agar (SDA) is used for isolate and identified dermatophyta fungus. Calculation and checking the total of leukocyte are using hemasitometer, while Giemsa liquid are using for differential leukocyte. T-Test shows the real differences between them which in to balinese cattle which infected by dermatophytosis. The analyzes result bye the statistic data using Mann-Whitney Test is showing there is real difference to balinese cattle’s monocyte which infected by dermatofitosis. There is a normal difference between balinese cattle which is normal and infected. Balinese cattle which infected dermatophyta fungus have a total of leukocyte and monocyte are higher than normal balinese cattle.
Derajat Keasaman dan Berat Jenis Urin sebagai Indikator Kajian Urolitiasis Pada Kucing Teresia Irene Julianta; I Putu Gede Yudhi Arjentinia; Putu Ayu Sisyawati Putriningsih
Buletin Veteriner Udayana Vol. 14 No. 1 February 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.981 KB) | DOI: 10.24843/bulvet.2022.v14.i01.p05

Abstract

Urolithiasis can be defined as the formation of sediment anywhere within the urinary tract. The main factor that regulates mineral crystallization and urolit formation is the degree of urine saturation with certain minerals. This study aims to determine the degree of acidity (pH) and specific gravity of urine (BJ) cats can serve as an indicator of urolithiasis incidence and know the type of urolith formed in cat urolithiasis. A total of 15 urine cat samples with urolithiasis were used in this study. Urine was tested by dipstick to observe changes in BJ and urinary pH and the examination of sediments by microscope to observe the urolith formed. The results show that the degree of acidity (pH) of urine can be used as an indicator to diagnose urolithiasis in cats, but specific gravity (BJ) of urine can not be used as an indicator to diagnose urolithiasis in cats and urolit that is formed urolit struvit and urolit calcium.
VAKSINASI RABIES DAN STERILISASI ANJING DI DESA MENDOYO DANGIN TUKAD, KECAMATAN MENDOYO, KABUPATEN JEMBRANA I.P.G.Y. Arjentinia; I.K.A. Dada; P.A.S. Putriningsih; I.W. Gorda; A.A.G. Jayawardhita; I.G.A.G.P. Pemayun; M.K. Budiasa; I.W. Batan
Buletin Udayana Mengabdi Vol 17 No 4 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.02 KB) | DOI: 10.24843/BUM.2018.v17.i04.p01

Abstract

Rabies adalah penyakit infeksi pada susunan saraf pusat disebabkan oleh virus rabies yang ditularkan anjing ke manusia. Rabies sudah menjadi kasus endemik di Bali. Kabupaten Jembrana merupakan kabupaten di Bali yang mempunyai kasus gigitan anjing terjangkit rabies cukup tinggi. Sampai bulan Agustus 2016, sebanyak 18 kasus gigitan anjing positif rabies, dengan satu orang korban meninggal dunia. Di desa Mendoyo Dangin Tukad, setiap rumah penduduk memelihara satu sampai dua ekor anjing, yang sebagian besar tidak diikat maupun dikandangkan. Kondisi wilayah yang cukup mendukung penyebaran penyakit rabies. Tujuan dari kegiatan pengabdian masyarakat adalah memberikan informasi mengenai penyakit rabies serta mengurangi perkembangbiakan Hewan Penular Rabies (HPR) sehingga dapat membatasi jumlah populasi HPR tanpa harus membunuh hewan tersebut. Hasil pengabdian kepada masyarakat adalah sebanyak 20 ekor divaksinasi dengan vaksin rabies, delapan ekor kastrasi anjing jantan, dan sepuluh ekor anjing betina dilakukan ovariohisterektomi. Pengetahuan masyarakat tentang penyakit rabies, cara pengendaliannya, dan pengendalian populasi sudah sangat bagus.
SOSIALISASI PENYAKIT ZOONOSIS ESCHERICHIA COLI O157:H7 SERTA PELAYANAN KESEHATAN SAPI DI DUSUN LAMPU DESA CATUR KINTAMANI BANGLI I W. Suardana; I.B.N. Swacita; I.N. Suartha; I G.N. Sudisma; M.D. Rudyanto; I.G.M. Krisna Erawan; I.N. Suarsana; I.W. Batan; P.A. Sisyawati Putriningsih; T. Sari Nindia; A.L.T. Rompis; I.N. Mantik Astawa; K. Karang Agustina; I.H. Utama; I.G.A. Suartini; I.M. Sukada; I.K. Suada; A.A.A. Mirah Adi
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.738 KB)

Abstract

Ternak sapi yang menderita diare berpeluang besar untuk ditemukan adanya agen zoonosis E. coli O157:H7 mengingat sapi sebagai reservoir utama dari agen tersebut.Transmisi penularan strain bakteri ini ke manusia umumnya terjadi melalui konsumsi daging yang kurang dimasak, produk susu yang tidak dipasteurisasi, air yang terkontaminasi feses. Dusun Lampu sebagai salah satu Dusun di Desa Catur merupakan salah satu daerah potensial untuk pengembangan ternak khususnya sapi sehingga menjadikan program pelayanan kesehatan di wilayah tersebut sangat potensial untuk dilakukan. Hasil kegiatan pengabdian masyarakat berupa sosialisasi penyakit zoonosis E. coli O157:H7 serta pelayanan kesehatan ternak sapi di Dusun ini, memperlihatkan respon positif yang dicirikan dengan cukup banyaknya jumlah ternak yang memperoleh pelayanan yaitu sejumlah 65 ekor sapi dari 35 petani ternak. Jenis pelayanan yang dilakukan meliputi tindakan spraying atau pemberian butox terhadap semua ternak sapi yaitu 65 ekor (100%), disusul dengan pemberian vitamin pada 52 ekor (80%), pemberian obat cacing sebanyak 39 ekor (60%), serta pemberian delladryl pada 1 ekor sapi (1,5%). Hasil ini mengindikasikan bahwa program pengabdian yang dilakukan cukup efektif dapat menyentuh kebutuhan dasar petani ternak, sehingga benar-benar dapat dirasakan manfaatnya.
Identifikasi Spesies Fungi Microsporum gypseum dan M. nanum Penyebab Ringworm pada Sapi Bali (IDENTIFICATION OF SPECIES FUNGI MICROSPORUM GYPSEUM Putu Ayu Sisyawati Putriningsih; I Putu Gede Yudhi Arjentinia
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.423 KB) | DOI: 10.19087/jveteriner.2018.19.2.177

Abstract

Ringworm is an zoonotic infectious skin disease that can infect many types of animals. This disease is caused by dermatophytes fungi. Ringworm cases in cattle were quite widely reported in various countries but a report about the dermatophytes fungi that caused ringworm in bali cattle have never been published. It is very important in the efforts to give more effective therapies. This study aimed to identify the species of dermatophytes fungi that caused ringworm in bali cattle. Eight bali cattles suspected ringworm samples were taken using superficial skin scraping and trichogram (hair pluck) technique. The samples of skin scrapings dan hair on the area of the lesion were taken for direct microscopic examination to find the presence of fungal elements (hyphae or arthrospora). They were dropped with 10% KOH, were allowed for 10-15 minutes, then were observed using microscope. Skin scrapings dan hair samples that showed positive results were cultured on Sabauroud’s Dextrose Agar (SDA) medium for 1-3 weeks dan were identified using Lactophenol Cotton Blue with microscope. The data were analyzed descriptively. Dermatophytes fungi that isolated dan identified were Microsporum gypseum (75% or 6/8) and Microsporum nanum (25% or 2/8). Both of these fungi have ectothrix invasion/infection tipe, which is the forming of arthrospores/arthroconidia is only on the surface/superficial hair shaft therefore the topical therapy is sufficient to resolve the infection.
Identifikasi dan Prevalensi Kejadian Ringworm pada Sapi Bali (IDENTIFICATION AND PREVALENCE OF RINGWORM CASE ON BALI CATTLE) Putu Ayu Sisyawati Putriningsih; Sri Kayati Widyastuti; I Putu Gede Yudhi Arjentinia; I Wayan Batan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.617 KB)

Abstract

Ringworm is an infectious skin disease caused by dermatophytes and can infected various of animals,including cattle. This disease is not only able to cause high economic losses in the farm but also zoonotic.The aims of this study were to conduct a preliminary study of ringworm in Bali cattle of especieally toidentify the causes and clinical lesion and also its prevalence. Samples which suspected ringworm werecollected from Badung and Buleleng Regency, Bali Province. Skin lesion of suspected infected by ringwormwere observed, recorded, and documented. Skin scrapings and hair was taken for direct microscopicexamination for the presence of fungal element (hyphae or arthrospora). Skin scrapings and hair sampleswere dropped with KOH 10% and China ink (Parker®) then were left at room temperature for 10-15minutes. Samples were observed by microscope with a magnification of 100X and 400X gradually. Thedata were analyzed descriptively. In skin scraping and hair samples were found fungal elements, butcannot identify the species. Clinical lesions are seen in Bali cattle infected with ringworm include white,gray, or black (hyperpigmentation) circular alopecia accompanied by hyperkeratosis, scale, and crusting.Ringworm prevalence on Bali cattle in Bali is about 1.48%.. The prevalence rates associated with weatherfactors and maintenance system.
Dilatated Cardiomyopathy In Two Month Old Puppy Haris Muhamad Ikhsan; Putu Ayu Sisyawati Putriningsih
Journal of Applied Veterinary Science And Technology Vol. 2 No. 2 (2021): October 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V2.I2.2021.43-49

Abstract

Background: Canine dilated cardiomyopathy (DCM) is a disease that results in a decreased ability of the cardiac contraction to generate pressure to pump blood through the vascular system. DCM is characterized by dilation of the ventricles with ventricular wall thinning. Purpose: The DCM case in Indonesia is rarely reported; therefore, this paper contains information about dilatated cardiomyopathy in a 2-month-old puppy. Case Analyze: A two-month-old local dog arrived with a complaint about coughing, loss of appetite, fatigue, and swelling on extremities, also having a history of seizures and bloody diarrhea. Physical examination shows that the patient breathes using abdominal type and polypnea, tachycardia pulse, pale mucose, and dehydration. Electrocardiogram result shows tachycardia sinus and abnormality in the depression of ST-segment. Radiography examination shows heart dilation and liquid accumulation in the thoracic cavity and abdomen. Hematology routine examination shows microcytic hyperchromic anemia, leucocytosis, and eosinophilia.  Feces examination resulted in negative. Pathology anatomy examination show dilatated cardio, pulmonum hepatization, fluid accumulation in the thoracic cavity and abdomen cavity. Result: According to anamnesis, clinical examination, laboratory examination, and anatomy pathology examination can be concluded that the dog, in this case, is diagnosed with dilatated cardiomyopathy.
Nilai vertebrae heart size anjing kintamani bali pada usia berbeda I Putu Gede Yudhi Arjentinia; Putu Ayu Sisyawati Putriningsih
ARSHI Veterinary Letters Vol. 2 No. 2 (2018): ARSHI Veterinary Letters - Mei 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.157 KB) | DOI: 10.29244/avl.2.2.33-34

Abstract

Anatomical physiological clinical studies such as cardiac image, is one of the important studies that must to strengthen the position of kintamani bali dog as a dog recognized by the FCI. Cardiac image that can be seen is the size of the cardiac based on the value of vertebral heart size (VHS). The results obtained are used as the standard size and assessment of cardiac of kintamani bali dogs. This study aims to determine the VHS value of kintamani bali dog based on age group 12 and 24 months. The sample used 40 kintamani bali dogs, such as 20 dogs of 12 months and 20 dogs of 24 months. Images were taken by X-ray to determine the value of VHS kintamani bali dogs. The method used a lateral thoracic radiograph. Measured by using calipers at the longest axis from cardiac silhouette from carina to the apex, and the short axis were measured from the widest part of the cardiac silhouette. Then transfer that measured to the vertebrae, starting at the cranial edge of T4, count the number of vertebrae that fall within the caliper points, and sum of the two measurements. The result showed that the values of VHS at 12 months kintamani dogs were 9.4v±1.6 and the 24 month were 9.4v±0.8
Co-Authors Adiari, Ni Made Santi Rahayu Aida Lousie Tenden Rompis Asyauqi Ilham Perdana, Asyauqi Ilham Aufa Prasetia, Ryan Br Sitepu, Dinda Meilinda Caroline, Grace Distira, Luh Ayu Yasendra DWI SURYANTO Haris Muhamad Ikhsan I Gede Soma I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Ngurah Sudisma I Ketut Mangku Budiasa I Ketut Puja I Ketut Suada I Made Agus Miyasa Jaya I Made Sukada I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Gede Yudhi Arjentinia I Wayan Batan I Wayan Suardana I Wayan Suardana I Wayan Syartama Hadi Nugraha I.G.A.G.P. Pemayun I.H. Utama I.K.A. Dada I.W. Batan I.W. Gorda Ida Bagus Ngurah Swacita Iwan Harjono Utama Jayawardhita, Anak Agung Gde Kadek Karang Agustina Khadafi, Gde Erwin Ali Khamid Yusuf Baehaqi, Khamid Yusuf M.D. Rudyanto MAS DJOKO RUDYANTO maulana, Ibrahim Negara, I Nyoman Wisnu Ni Putu Vidia Tiara Timur Nirae Nirae Nurani Prabawa, I Made Agus Pratama, Dimas Andi Purnama, Komang Andika Putu Devi Jayanti Rate, I Made Bagi Resman, Martin Pedro Krisenda Ryan Aufa Prasetia Samosir, Hartina Sri Kayati Widyastuti T. Sari Nindia Teresia Irene Julianta Vonny, Ni Ketut Wiadnyani, Kadek Ayu Wibisono, Hanif Wahyu