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Formulasi Krim Anti-Aging Dari Ekstrak Etanol Bawang Merah (Allium cepa L.) Maya Amelia Sinaga; Vivi Asfianti; Kasta Gurning
Herbal Medicine Journal Vol 3 No 1 (2020): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.662 KB)

Bawang merah yang merupakan spesies Allium cepa L adalah nama tanaman dari familia Amaryllidaceae. Bawang merah menyediakan sekitar 29% dari flavonoid yang diperlukan tubuh sekaligus membuktikan bahwa bawang merah merupakan sumber polifenol antioksidan yang baik. Dalam survey terhadap 29 sayuran dan buah-buahan, bawang merah menduduki peringkat tertinggi kandungan kuersetin. Kuersetin merupakan senyawa flavonoid dari kelompok flavonol dan diindikasikan sebagai fitokimia flavonoid yang mempunyai kemampuan antioksidan paling kuat. Tujuan penelitian ini adalah untuk mengetahui apakah ekstrak etanol bawang merah dapat diformulasikan dalam sediaan krim anti-aging dan untuk mengetahui kemampuan ekstrak etanol bawang merah sebagai anti-aging. Bawang merah diekstraksi dengan maserasi menggunakan pelarut etanol 96% ekstrak kemudian dipekatkan menggunakan rotary evaporator. Konsentrasi ekstrak bawang merah yang digunakan dalam sediaan adalah 1,3, dan 5% lalu dibandingkan dengan sediaan Pond’s dan blanko (tanpa ekstrak bawang merah). Evaluasi sediaan yang dilakukan adalah pemeriksaan homogenitas, penetuan tipe emulsi, pH, uji iritasi, dan tidak mengalami perubahan selama 12 minggu. Dan diproleh hasil pemeriksaan menggunakan skin analyzer yang paling efektif adalah konsentrasi 5%. Secara keseluruhan dapat disimpulkan bahwa ekstrak bawang merah dapat diformulasikan kedalam sediaan krim dan memiliki kempuan sebagai anti-aging.

Molecular docking analysis of six major compounds from sweet basil (Ocimum basilicum L.) extract as potential anti-hypertension therapy Bayu Cakra Buana; Iksen Iksen; Kasta Gurning
Jurnal Pendidikan Kimia (JPKIM) Vol 15, No 2 (2023): August
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v15i2.43617

Hypertension is an abnormally high blood pressure condition that is the leading cause of preventable cardiovascular disease, chronic kidney disease, and cognitive impairment. In the case of hypertension, repressing the Angiotensin-Converting Enzyme (ACE) expression has been shown to be an effective method of controlling hypertension by inhibiting the conversion of angiotensin I to angiotensin II. Captopril is the most commonly used ACE inhibitor. It simultaneously inhibits the conversion of angiotensin I to the potent vasoconstrictor angiotensin II and the vasodilator peptide bradykinin. Sweet basil (Ocimum basilicum L.) on the other hand, is used in traditional Indian and Chinese medicine to treat a variety of diseases, including hypertension. The study aimed attempts to investigate the potency of 6 major compounds found in sweet basil (Ocimum basilicum L.) extract, as an anti-hypertension therapy. The analysis demonstrates that Ocimum basilicum L., extract is effective as an anti-hypertension therapy because it contains several compounds that may interact with ACE and inhibit its activity. The molecular docking analysis and drug-likeness prediction indicate that camphor could be a potential drug candidate because it does not violate the Lipinski rule, has a high Gastrointestinal (GI) absorption, a high affinity to interact with ACE, and a similar interaction site to the ACE-Captopril interaction.Keywords: ACE; Holly basil; Hypertension; Molecular docking

Phytochemical Screening and Anti-Hyperuricemia Activity Test In Vivo of Ethanolic Extract of Shallot (Allium cepa L.) Skin Syahrina Syahrina; Vivi Asfianti; Kasta Gurning; Iksen Iksen
Borneo Journal of Pharmacy Vol. 3 No. 3 (2020): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v3i3.1365

Uric acid is the final product of purine metabolism that will be excreted through urine, feces, and sweat. Excessive production of uric acid can cause hyperuricemia, known as gout. The skin of shallots (Allium cepa L.) is one of the household wastes that are very rarely used by the community. Ethanol extract of shallot skin (EESS) was tested for phytochemical screening and anti-hyperuricemia activity using potassium oxonate. Mice were divided into five groups (Allopurinol, Na-CMC, EESS 200 mg/kg BW, 300 mg/kg BW, and 400 mg/kg BW) and uric acid levels were observed at 2-hour intervals for six hours. Phytochemical screening shows that EESS has potential compounds in the treatment of gout. Tests to reduce uric acid levels showed that EESS has better potential than allopurinol at concentrations of 300 mg/kg BW and 400 mg/kg BW after six hours of induction in reducing uric acid levels.

Uji Aktivitas Antibakteri Sediaan Bedak Dingin Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi Linn.) Terhadap Propionibacterium acnes || Antibacterial Activity of Cold Powder Preparation of (Ethanol Extract) Starfruit Leaf (Averrhoa bilimbi Linn.) Against Propionibacterium acnes Simanjuntak, Helen Anjelina; Gurning, Kasta; Sinaga, Verawati Br.
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 6, No 2: Jurnal Pembelajaran Dan Biologi Nukleus September 2020
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v6i2.1677

Jerawat adalah penyakit kulit yang terjadi akibat adanya peradangan menahun yang dipicu oleh bakteri Propionibacterium acnes. Daun belimbing wuluh (Averrhoa bilimbi Linn.) merupakan tanaman obat tradisional yang memiliki aktivitas sebagai antibakteri. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri dari sediaan bedak dingin ekstrak etanol daun belimbing wuluh (A. bilimbi Linn.) terhadap Propionibacterium acnes. Metode penelitian dilakukan dengan eksperimental, dan pengujian aktivitas antibakteri dilakukan dengan menggunakan metode difusi agar. Formulasi bedak dingin F1 (5%), F2 (10%), F3 (15%), F4 (20%), kontrol positif dengan klindamisin, kontrol negatif dengan akuades. Hasil menunjukkan bahwa formulasi sediaan F3 (15%) dan F4 (20%) lebih efektif dalam menghambat pertumbuhan bakteri Propionibacterium acnes dengan diameter zona hambat 13,66 mm dan 15,16 mm dengan kategori kuatAcne is a skin disease that occurs due to chronic trade that is triggered by the bacterium Propionibacterium acnes. Starfruit leaf (Averrhoa bilimbi Linn.) as a traditional medicinal plant that has antibacterial activity. The purpose of this study was to determine the antibacterial activity from ethanol extract in the preparations cold powder of starfruit leaf (A. bilimbi Linn.) against Propionibacterium acnes. The research method was carried out experimentally, and testing for antibacterial activity was carried out using agar diffusion methods. Cold powder formulation such as F1 (5%), F2 (10%), F3 (15%), F4 (20%), positive control with clindamycin, negative control with distilled water. The results showed that the F3 (15%) and F4 (20%) formulations were more effective in inhibiting the growth of Propionibacterium acnes bacteria with inhibition zone diameters of 13.66 mm and 15.16 mm in the strong category.

Fight for Cancer Diseases using Natural Compounds and Their Semisynthetic Derivatives Yehezkiel Steven Kurniawan; Kasta Gurning; Iksen Iksen; Ahmad Bikharudin
Bioactivities Vol. 2 No. 2 (2024): Bioactivities
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/bioactivities.2963-654X.221

Cancer stands as one of the deadliest diseases afflicting contemporary human societies, encompassing over 100 distinct forms, including oral, salivary glands, oropharynx, nasopharynx, hypopharynx, colorectal, liver, pancreas, lung, skin, breast, cervix, ovary, prostate, kidney, brain, thyroid, and leukemia cancers. At the cellular level, the uncontrolled growth of cancerous cells can disrupt the body's normal functions. Chemotherapy, a widely recognized cancer treatment, utilizes anticancer agents to target specific cancer cell lines effectively. Natural compounds are favored for their compatibility with the body, minimal harm to healthy cells, and easy extraction from natural sources. These natural compounds and their derivatives hold promise for cancer therapy, boasting diverse structural and pharmacological characteristics. Additionally, chemical modifications can enhance their anticancer properties. This review explores the anticancer potential of terpenoids, flavonoids, alkaloids, xanthones, and epoxides, as well as elucidates their molecular mechanisms, such as their antiproliferative, apoptotic, antiangiogenic, and antimetastatic actions. Furthermore, the effect of the functional group on the anticancer activity through the structure-activity relationship will be discussed in detail, supported by molecular docking and molecular dynamic simulations. Combining experimental in vitro and in vivo assays with computational in silico assays significantly helps us understand how we shall fight cancer diseases in the modern era.

Profiling and Antibacterial Activity Assay of Secondary Metabolites from Streptomyces Isolated from Mangrove Sediment Sample Dwi Marwati Juli Siswanti; Kasta Gurning; Winarto Haryadi; Chairil Anwar; Respati Tri Swasono
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.236

Bacterial infections pose significant threats to human health, particularly due to the rise of antibiotic-resistant strains. Identifying new sources of effective antibiotics is therefore crucial for combating these resistant pathogens. This study aims to isolate novel Streptomyces species and profile their secondary metabolites through extraction and high-resolution mass spectrometry (HRMS) analysis. Furthermore, the antibacterial activity of the extract containing the secondary metabolites was assessed through the in vitro agar well diffusion method and supported by the molecular docking, molecular dynamics simulations, and drug-likeness analysis. Sediment samples were collected from mangrove forests in Yogyakarta. The bacteria were then isolated, purified, and characterized using 16S rRNA sequencing. The isolates were then cultured to enrich the secondary metabolites, and their secondary metabolites were extracted using methanol and dichloromethane solvents in a 1:1 volume ratio. The results showed that the isolated bacteria of Streptomyces sp. were obtained with a 95.44% similarity rate, which produced several secondary metabolites. The in vitro antibacterial assay of the extract resulted in an inhibition zone of 14, 14, and 15 mm against Propionibacterium acnes, Staphylococcus aureus, and Escherichia coli, respectively. The molecular docking and molecular dynamic simulations for 100 ns revealed that compound SB236057A could inhibit the function of thymidylate kinase protein through a carbon-hydrogen bond with Glu37 residue. Furthermore, drug-likeness analysis showed that the secondary metabolites of Streptomyces sp. exhibited preferable drug-likeness and pharmacokinetic properties. This research focuses on the understanding of microbial biodiversity in mangrove sediments, particularly focusing on the genus Streptomyces and its potential to produce novel antibiotics.

Potential of Bioactive Compounds In Coleus amboinicus, Lour., Leaves Against Breast Cancer By Assessment Using A Network Pharmacology Approach and Cytotoxic Test Winarto Haryadi; Kasta Gurning; Jajah Fachiroh; Endang Astuti
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.246

Breast cancer is a disease that significantly contributes to global women death. The study aims to conduct in vitro activity testing and assessment with a bioinformatics approach using a pharmacological network of bioactive compounds from bangun-bangun (Coleus amboinicus) leaves extract as a breast cancer drug. The methods used are extraction of bioactive compounds by maceration and partition, identification and analysis of bioactive compounds using the Liquid Chromatography High-Resolution Mass Spectrometry (LC-HRMS) instrument, cytotoxic testing of breast cancer cells (MCF-7) and normal cells (CV-1) with the MTT method, and assessment with a bioinformatics approach through a network pharmacology. The results of the cytotoxic test of ethyl acetate extract provided better activity with IC50 value of 102.30 and 457.09 µg/mL against MCF-7 cancer cells and CV-1 normal cells. The selectivity index value of 4.23 indicates the potential for further development in the treatment of breast cancer. The results of the analysis of chemical compound content show various types of potential bioactive compounds as breast cancer anticancer; assessment of the bioinformatics approach through networks pharmacology with pathways in cancer provides predictions of signal transducer and activator of transcription 3 (STAT3) protein as the main therapeutic mechanism target in breast cancer treatment. This study provides initial information for further research on testing and utilizing bioactive compounds from C. amboinicus leaves as an alternative treatment for breast cancer.

Synthesis of (3E,5E)-1-benzil-3,5 bis (3 (benziloksi)benziliden)piperidin-4-on curcumin analogues and their potential as breast anticancer agents: Assessment using MTT test and molecular docking Endang Astuti; Jihan Alfiyah Kultsum; Zarah Aulia; Frika Rahmawari; Kasta Gurning; Sugeng Triono; Winarto Haryadi; Harno Dwi Pranowo
Jurnal Pendidikan Kimia Vol. 16 No. 3 (2024): J. Pendidik. Kim : December 2024
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v16i3.65150

Breast cancer is a serious disease that occurs in women and contributes to the highest mortality compared to other types of cancer. This study aims to synthesize curcumin analog compounds ((3E,5E)-1-benzyl-3,5 bis (3 (benzyloxy)benzylidene) piperidin-4-one), test them in vitro against various breast cancer cells (T47D, HER-2, MCF-7, and 4T1) and normal cells (vero cells), and study their molecular docking. Synthesis was carried out by reacting 3-benzyloxybenzaldehyde with N-benzyl-4-piperidone catalyzed by 5% KOH at room temperature; in vitro testing was carried out using the Microculture Tetrazolium Technique Assay method, ADMET analysis with an online database server, and molecular docking studies in Autodoc Vina. The synthesis results obtained yellow solid powder with a yield of 65.85%, characterization with TLC gave black fluorescence (Rf 0.63), melting point 114-116oC, TLC scanner one peak (100%), retention time 0.65 minutes, 1H &13C-NMR analysis showed the molecular formula C40H35NO3, moderate activity against 4T1 breast cancer cells and inactivity on T47D, HER-2, and MCF-7 cancer cells, and did not show cytotoxicity to normal cells (vero cells). ADMET predictions from Lipinski's five rules contained two parameters that did not meet, namely molecular weight and log P value. Molecular docking studies were carried out on estrogen receptor protein (ER)-α (PDB ID: 2ERT), which showed a binding affinity energy of -8.7 kcal/mol and -7.1 kcal/mol of the native ligand. Further research and development is needed on synthetic curcumin analog compounds to increase their activity value against breast cancer by paying attention to Lipinski's five rules to obtain compounds with better potential activity and ADMET.

Synthesis of (3E,5E)-1-benzil-3,5 bis (3 (benziloksi)benziliden)piperidin-4-on curcumin analogues and their potential as breast anticancer agents: Assessment using MTT test and molecular docking Astuti, Endang; Kultsum, Jihan Alfiyah; Aulia, Zarah; Rahmawari, Frika; Gurning, Kasta; Triono, Sugeng; Haryadi, Winarto; Pranowo, Harno Dwi
Jurnal Pendidikan Kimia Vol. 16 No. 3 (2024): J. Pendidik. Kim : December 2024
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v16i3.65150

Breast cancer is a serious disease that occurs in women and contributes to the highest mortality compared to other types of cancer. This study aims to synthesize curcumin analog compounds ((3E,5E)-1-benzyl-3,5 bis (3 (benzyloxy)benzylidene) piperidin-4-one), test them in vitro against various breast cancer cells (T47D, HER-2, MCF-7, and 4T1) and normal cells (vero cells), and study their molecular docking. Synthesis was carried out by reacting 3-benzyloxybenzaldehyde with N-benzyl-4-piperidone catalyzed by 5% KOH at room temperature; in vitro testing was carried out using the Microculture Tetrazolium Technique Assay method, ADMET analysis with an online database server, and molecular docking studies in Autodoc Vina. The synthesis results obtained yellow solid powder with a yield of 65.85%, characterization with TLC gave black fluorescence (Rf 0.63), melting point 114-116oC, TLC scanner one peak (100%), retention time 0.65 minutes, 1H &13C-NMR analysis showed the molecular formula C40H35NO3, moderate activity against 4T1 breast cancer cells and inactivity on T47D, HER-2, and MCF-7 cancer cells, and did not show cytotoxicity to normal cells (vero cells). ADMET predictions from Lipinski's five rules contained two parameters that did not meet, namely molecular weight and log P value. Molecular docking studies were carried out on estrogen receptor protein (ER)-α (PDB ID: 2ERT), which showed a binding affinity energy of -8.7 kcal/mol and -7.1 kcal/mol of the native ligand. Further research and development is needed on synthetic curcumin analog compounds to increase their activity value against breast cancer by paying attention to Lipinski's five rules to obtain compounds with better potential activity and ADMET.

Perbandingan Pengaruh Konsumsi Jus Terong Belanda Segar dan Komersil Terhadap Tikus Obesitas Syahputra, Hariyadi Dharmawan; Harahap, Daimah Wirdatus Sanaun; Iksen, Iksen; Gurning, Kasta
Herbal Medicine Journal Vol 8 No 1 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i1.144

Background: High yield carbohydrate diet is common in Indonesia mainly in North Sumatra province. In the long run consuming primary carbohydrate form rice may increase the risk for diabetic and its complication. Not only the amount of rice but certain beverages also such as tamarillo juice, though it’s rich amount of nutritious benefit, some product may yield more sugar to improve from its sour or bitter flavor. Objectives: studies aim to find the diet correlation between amount of carbohydrate total and Vitamin C intake in fresh and store brought tamarillo juice affect animal model with high sucrose induction. Material and Methods: Fresh tamarillo fruit grew in north Sumatra blended versus store brought juice are given daily towards obese female rats induced with 2 ml of lard and 5% sucrose orally for five weeks. Beforehand sample had its total carbohydrate and Vitamin C content with iodometric method. The last 2 weeks then rats treated with fresh blend and store brought product juice. Results: Total content value obtained from fresh compared with store brought tamarillo juice in vitamin C, (18.63 ± 7.16 mg/ml); (11.57 ± 6.8 mg/ml) and total carbohydrate (15.02 ± 2.35 mg/ml); (25.37 ± 28.17 mg/ml) respectively. During treatment increase in body weight significantly in store brought product. Histology remarks in pancreas do not show any significance damage in cell especially for fresh blend treatment group. Conclusion: Fresh blend tamarillo juice may improve health and can be considered safe for subject that like to consume high carbs in their diet.

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