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All Journal Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Parasite Science
Wiwik Misaco Yuniarti
Divisi Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary Other

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Journal : Jurnal Veteriner

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Terapi Fotodinamik Mempercepat Kesembuhan Luka Insisi pada Kulit Tikus Putih (Rattus novergicus) yang Diinfeksi Bakteri Methicilin-Resistant Staphylococcus aureus Yonatan Dimascahyo Budianto; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Hani Plumeriastuti; Wiwiek Tyasningsih; Boedi Setiawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research was to determine the effect of photodynamic therapy (PDT) on the healing of incision wounds in rat skin (Rattus norvegicus) that infected with MRSA bacteria by evaluating and give a score (0 – 4) at wound healing factors such as epithelialization, angiogenesis, inflammatory cells, fibroblast cells and collagen density. This study used 25 white rats divided randomly into 5 groups with 5 replications, namely P0 = MRSA infection wound + Povidone Iodine, P1 = MRSA infection wound + Silver Sulfadiazine, P2 = MRSA infection wound + 5-minute PDT irradiation, P3 = MRSA infection wound + 10- minute PDT irradiation and P4 = MRSA infection wound + 15-minute PDT irradiation. Histopathological observations and scoring showed epithelialization and collagen density in groups P2, P3 and P4 increased significantly compared to groups P0 and P1 (p<0.05). The histopathological observations and scoring of angiogenesis, inflammatory cells, fibroblast cells of P0 and P1 groups had a higher score than the treatment group with photodynamic therapy P2, P3 and P4 (p<0.05). This condition showed that in the P0 and P1 groups are still in the inflammatory phase, while in the P2, P3 and P4 groups have entered the remodeling phase which is characterized by high epithelialization scores and collagen density. Giving photodynamic therapy to wounds infected with MRSA can accelerate the wound healing process.
Deteksi Molekuler Blastocystis sp. pada Babi Terinfeksi di Kabupaten Tabanan dan Badung, Provinsi Bali, Indonesia (MOLECULAR DETECTION OF BLASTOCYSTIS INFECTION IN PIGS AT TABANAN AND BADUNG DISTRICT, BALI PROVINCE, INDONESIA) Doohan Mahendra; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Endang Suprihati; Wiwik Misaco Yuniarti; Ni Komang Aprilina Widisuputri
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims to identify morphology and molecular of Blastocystis infection in pigs in the Tabanan and Badung District, Bali Province, Indonesia. 100 samples of pig stool were taken, fifty samples from the Tabanan and fifty samples from Badung District. Blastocystis sp. isolated and identified microscopically and confirmed by polymerase chain reaction (PCR) as a molecular identification method. 63% (63/100) Blastocystis is found in pig feces from these two District by microscopic detection. Positive samples were found 27 samples from Badung and 36 samples from Tabanan. Five samples were taken from each District conducted a confirmation test using the PCR method and found 60% (6/10) positive samples, two samples from Badung and four samples from Tabanan. The results of this study indicate that in healthy pigs, they also contain parasites that have the potential to spread disease to humans. Therefore, the high incidence of Blastocystis in pigs can be an alarm or warning to prevent Blastocystis infection from livestock that can potentially to be a reservoir in the spread of disease.
Potensi Salep Epigallocatechin gallate terhadap Proses Kesembuhan Luka Bakar Derajat II pada Kulit Tikus Putih (THE POTENTIAL OF EPIGALLOCATECHIN GALLATE OINTMENT TO THE WOUND HEALING PROCESS OF SECOND DEGREE SKIN BURNS ON THE ALBINO RATS) Wiwik Misaco Yuniati; Bambang Sektari Lukiswanto
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.331 KB) | DOI: 10.19087/jveteriner.2019.20.1.1

Abstract

Burns are one of the health problems in modern society that are associated with tissue damage that is difficult to repair and affect patients, both physically and psychologically. This study was conducted to evaluate the potential of epigallocatechin gallate (EGCG ) ointment to the healing process of second degree skin burn induced by attaching 85ºC plate with 1 cm of diameter for 5 second on the skin of albino rat (Rattus norvegicus). Twenty-five rats were divided into 5 treatment groups. The P0 group was a group of rat that suffered burns and were treated with ointment base (PEG). The P1 group was a group of rat that suffered burns and were given standard therapy with silver sulfadiazine. P2, P3 and P4 groups are groups of rat that have burns and are treated with EGCG ointments with concentrations of 1%, 2%, and 4% respectively. At the end of the study, skin tissue excision was carried out to make histopathological preparations using HE staining. Evaluation of histopathological preparations was carried out on reepithelialization collagen deposition, PMN infiltration, and angiogenesis. The results of the study in group P4 showed that the highest collagen formation and re-epithelialization process was accompanied by a marked decrease in the inflammatory process and angiogenesis. This condition is significantly different from groups P0, P1, P2, and P3. Healing second degree burns with 4% EGCG is better than other treatmentJurnal Veteriner Maret 2019 Vol. 1 No. 1 : 1-7 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.1 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/20162Proses kesembuhan luka bakar merupakan fenomena kompleks untuk mengembalikan kontinuitas jaringan dan fungsinya. Kesembuhan luka melibatkan beberapa fase yang berbeda dan saling tumpang tindih, yaitu fase inflamasi, granulasi, fibrogenesis, neo-vaskularisasi, kontraksi luka dan epithelialisasi (Robson, 1997). Penatalaksanaan luka bakar yang efektif memerlukan pemahaman proses kesembuhan luka normal dan mampu memilih intervensi yang tepat untuk mengoptimalkan proses kesembuhan luka (Snyder, 2005). Pada proses kesembuhan luka, inflamasi terjadi segera setelah jejas, diawali dengan vasokonstriksi yang berperan dalam proses hemostasis dan pelepasan mediator inflamasi. Fase proliferasi ditandai dengan terbentuknya jaringan granulasi oleh fibroblas dan proses angiogenesis. Reformulasi dan perbaikan kompartemen serabut kolagen yang disertai dengan penigkatan tensile strength menandai fase remodeling (Varoglu et al., 2010). Faktor yang memiliki peran penting pada tertundanya proses kesembuhan luka antara lain, trauma berulang, perfusi dan oksigenasi yang buruk serta inflamasi yang berlebihan (Harding et al., 2003)). Penggunaan bahan alamiah untuk pengobatan luka merupakan bagian penting dari penatalaksanaan kesehatan dan metode baik untuk menyediakan pilihan layanan kesehatan yang murah dan efektif (Gurung et al., 2009; Suntar et al., 2010). Beberapa penelitian menggunakan polifenol yang berasal dari teh hijau sebagai penyembuh alami sebagai agen anti penuaan, antiinflamasi, antikanker, antioksidan dan antidiabetes (Obaid et al., 2011). Beberapa penelitian dengan menggunakan Epigallocatechin gallate (EGCG), sebagai salah satu polifenol yang terkandung dalamteh hijau, membuktikan sejumlah efek biologis EGCG sebagai antioksidan, antimikrob, antiinflamasi, antialergi dan antineoplastik yang aktif (Hosnuter et al., 2015). Teh hijau memiliki manfaat yang beragam, antara lain mencegah kanker, meningkatkan kesehatan jantung dan pembuluh darah, melindungi kulit dari kerusakan yang disebabkan karena radiasi dan penyebab yang lain. Manfaat ini disebabkan karena the hijau yang mengandung EGCG memiliki aktivitas antioksidan, antiinflamasi dan antibakteri yang cukup kuat. Berdasarkan hal tersebut, tidak menutup kemungkinan bahwa EGCG dapat membantu percepatan proses kesembuhan luka bakar pada kulit (Nagle et al., 2006). Berdasarkan uraian tersebut, penelitian ini dilaksanakan untuk melihat pengaruh pemberian salep EGCG terhadap proses kesembuhan luka bakar pada tikus putih (Rattus norvegicus) jantan.METODE PENELITIAN Kelayakan Etik Penelitian ini dilaksanakan setelah mendapatkan persetujuan dari komisi etik Fakultas Kedokteran Hewan, Universitas Airlangga. Tempat Penelitian Penelitian ini dilakukan di Laboratorium Hewan Percobaan di Fakultas Kedokteran Universitas Airlangga, Surabaya. Persiapan dan pembuatan salep dilakukan di Fakultas Farmasi, Universitas Airlangga. Pembuatan dan pemeriksaan preparat histopatologis dilakukan di Laboratorium Patologi Anatomi, Fakultas Kedokteran Hewan, Universitas Airlangga. Hewan Eksperimental Sebanyak 25 ekor tikus putih (Rattus norvegicus) jantan, umur tiga bulan dengangroups. This is presumably because the compounds contained therein have antioxidant activity, antiinflammatory and antibacterial. These three activities will synergize in the process of healing wounds. Provision of 4% EGCG ointment for 14 days in second degree burns can accelerate the wound healing process which is characterized by improved re-epithelialization, collagen deposition, PMN infiltration in the wound area, and angiogenesis.
Co-Authors Amara Lintang Pagati Arimbi Arimbi Bambang Sektari Lukiswanto Bambang Sektiari Lukiswanto Boedi Setiawan Doohan Mahendra Doohan Mahendra Edward Yonas Kristijanto Elita Triwijayanti Aqfari Emy Koestanti Sabdoningrum Endang Suprihati Endang Suprihati Hani Plumeriastuti Hesty Aulia Palupi Iswinarno Doso Saputro Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Mufasirin Ni Komang Aprilina Widisuputri Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nusdianto Triakoso Poedji Hastutiek Puput Ade Wahyuningtyas Sarmanu, Sarmanu Soeharsono Soeharsono Sri Hidanah Tyasningsih, Wiwiek Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari Yonatan Dimascahyo Budianto