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All Journal Jurnal Ilmu Pertanian Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Pharmaceutical Sciences and Research (PSR) Annales Bogorienses
SHANTI RATNAKOMALA
Pusat Penelitian Bioteknologi-LIP
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Annales Bogorienses

 1 
Catechin Contents, Antioxidant, and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis) Nuryana, Isa; Ratnakomala, Shanti; Fahrurrozi, Fahrurrozi; Juanssilfero, Ario Betha; Andriani, Ade; Putra, Filemon Jalu Nusantara; Rezamela, Erdiansyah; Wulansari, Restu; Prawira-Atmaja, M. Iqbal; Lisdiyanti, Puspita
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of 5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties.
Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia Susanti, Agustina Eko; Ratnakomala, Shanti; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
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Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.
Rapid Assessment of Diverse Trichodermal Isolates of Indonesian Origin for Cellulase Production Fahrurrozi, Fahrurrozi; Ratnakomala, Shanti; Lisdiyanti, Puspita; Anindyawati, Trisanti; Sukara, Endang
Annales Bogorienses Vol. 14 No. 1 (2010): Annales Bogorienses
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Trichoderma is a well-known candidate to be promoted as cellulase producer for the hidrolysis of lignocellulose that contain in woody biomass. The number of trichodermal isolates in our laboratory collected from diverse ecosystem types in Indonesia increases significantly during the last 5 years. It is our aim to assess the cultures for its ability in producing cellulase. Sixty-six trichodermal isolates used in this experiment are obtained from Biotechnology Culture Collection (BTCC), Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI). The 31 isolates were isolated from District of Liwa (South Sumatra, Indonesia) and the 35 isolates from District of Maros (South Sulawesi, Indonesia). The isolates were screened qualitatively, 7 isolates from Liwa and 12 isolates from Maros showed cellulolytic activity. From the results of quantitative test, two strains (ID08-T004 and ID08-T63) showed the higher cellulolytic activity among the selected strains, 133.5 and 133.5 U/ml, respectively. These extracellular enzymes were characterized their temperature and pH optimum. The temperature optimum for both enzymes was the same, 50C, with activity 213.6 U/ml for enzyme extracted from ID08-T004 and 197.3 U/ml for enzyme from ID08-T0063. The pH optimum was pH 5 of ID08-T004 with activity 137.7 U/ml and pH 6 for ID08-T063 with activity 75.0 U/ml. The enzymes from ID08-T004 and ID08-T063 were stable in their temperature and pH optimal condition even after 90 minutes incubation with activity 179.0 U/ml and 86.7 U/ml, respectively. The enzyme stability was approximately 150 minutes for both enzymes in the temperature and pH optimum.
Ecological Study of Rare-Actinomycetes in Soils and Leaf-Litters Lisdiyanti, Puspita; Ratnakomala, Shanti; Ridwan, Roni; Widyastuti, Yantyati; Otoguro, Misa; Ando, Katsuhiko
Annales Bogorienses Vol. 15 No. 2 (2011): Annales Bogorienses
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A total of 268 strains of Actinomycetes were isolated from soil and leaf-litter samples collected in Jambi (Sumatera) and Cibinong (West Java) using three selective isolation methods. All the isolates were identified by morphological characteristic and by analysis of 16S rDNA sequence. On the basis of their morphology and 16S rDNA sequence, 164 isolates were belonged to the Streptomyces Group and 104 isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Furthermore, 40% of isolates in Streptomyces Group and 62% of isolates in Non-Streptomyces Group are supposed to be new taxa. It is indicated the richness of Actinomycetes in these two area. The genus Streptomyces is the most abundant in soil samples, occupying 60-75% of all isolates; while the genus Actinoplanes is mainly found in leaf-litter samples (70%). The use of selective isolation media is important to elucidate the microbial diversity.
Diversity of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita; Tamura, Tomohiko; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Lestari, Yulin; Ando, Katsuhiko; Widyastuti, Yantyati
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
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A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia using SDS-YE (SY) and Rehydration and Centrifugation (RC) isolation methods and Humic Acid Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus Streptomyces is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genus Actinoplanes is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.
Isolation and Characterization of Bacterial Carbonate Precipitation for Biogrouting Suyanto, Eko; Sari, Miranti Nurindah; Ratnakomala, Shanti; Fahrurrozi, Fahrurrozi; Gusmawati, Niken F.
Annales Bogorienses Vol. 25 No. 2 (2021): Annales Bogorienses
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Biogrouting is a process that transforms soil or sand into calcarenite or sandstone by bacterial calcium carbonate precipitation . The mechanisms of this process are that the bacteria hydrolyze urea catalyzed by urease, and with the existence of dissolved Ca2+, a solid crystalline calcite or calcium carbonate (CaCO3) will be produced. The main advantage of biogrout is that soil or sand can be strengthened without interfering with the hydraulics of the treated soil or sand and without excavation or replacement. In this study, the isolation and identification of bacterial calcium carbonate precipitation , and characterization of urease produced by bacteria were conducted. In the isolation method, the enrichment method using urea as abacterial carbon source was carried out. The formation of crystalline calcite was observed by a light microscope. The urease enzyme activity was determined by the 3, 5-Dinitrosalicylic acid (DNS) method. The molecular identification of isolates was analyzed by the determination of 16S rRNA gene sequencing. As a result, 19 calcium carbonate precipitation have been isolated from soils, sands, water, and rocks collected from several areas in Indonesia. They showed calcite formation in a medium with urea. Molecular identification of isolates with high urease enzyme activity revealed that the isolates belong to the group Bacilli. The highest enzyme activity produced by ID10-U004 is 374.94 U/mL. The preliminary biogrouting experiment was also conducted using isolated strain, and the reaction was able to make the sand solid and stiff.
Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti; Yopi, Yopi; Suhartono, Maggy Thenawidjaja; Meryandini, Anja; Prasetya, Bambang
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
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The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml), (NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28C for 2 days incubation.
Antimicrobial Activities of Actinobacteria Isolated from Marine and Terrestrial Samples in Lombok Island, Indonesia Ratnakomala, Shanti; Krause, Janina; Sari, Miranti Nurindah; Kusumawardhani, Dinihari Indah; Kusmiati, Mia; Lisdiyanti, Puspita
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.28-36

Abstract

During our study on exploring actinobacteria from Indonesian sources, we selected 59 strains of actinobacteria isolated with five different isolation methods from Lombok Island Indonesia. The aim of this research was to isolate and characterize those actinobacteria, and to screen the microbial activities against 5 bacterial tested. Six soil samples from Lombok Island were processed. Totally, 59 isolated were selected. Of these strains, 31 strains showed antibiotic activity. Seventeen strains from 59 strains have been identified by 16S rRNA gene sequencing, and most dominant of the strains belongs to the genus Streptomyces. Three of them were chosen for further experiments due to their broad activity against four of five test strains. From two strains, at least one bioactive substance could be extracted. As only few bioactive substances could be extracted with ethyl acetate from liquid cultures more experiments should be performed on this subject. More solvent extraction should be tested as well as other production media. In conclusion, present study did now reveal bioactive actinobacteria were isolated from soil samples in Lombok Island. Antimicrobial compound has also been detected which is active against a broad range of pathogens. These findings may have an importance to produce potentially new antibiotic substances from actinobacteria indigenous Indonesia.
Co-Authors Abd. Rasyid Syamsuri ADE ANDRIANI ANDI UTAMA ANDO KATSUHIKO Anja Meryandini Apriliana, Pamella Ario Betha Juanssilfero Atikana, Akhirta Bambang Prasetya Eko Suyanto Endang Sukara Erdiansyah Rezamela, Erdiansyah Evi Triana Fahrurrozi Fahrurrozi GINA KARTINA Gusmawati, Niken F. Hastuti, Ratih Dwi Herman Suryadi KATSUHIKO ANDO Krause, Janina Kusharyoto, Wien Kusumawardhani, Dinihari Indah Lina Elfita LINDA SUKMARINI Lisdayanti, Puspita M. Iqbal Prawira-Atmaja, M. Iqbal Maggy T Suhartono Maggy Thenawidjaja Suhartono Maulidia, Faiqoh Nur Mia Kusmiati Miranti Nurindah Sari, Miranti Nurindah MISA OTOGURO Mulyorini Rahayuningsih Nadhifah, Hana NANIK RAHMANI Neneng Hasanah Ni Luh Putu Vidya Paramita NITA ROSALINDA PRAYITNO Nuryana, Isa Prayoga, Muhammad Bagas PUSPITA LISDIYANTI Putra, Filemon Jalu Nusantara Restu Wulansari, Restu RONI RIDWAN Susanti, Agustina Eko Titik Nuryastuti TOMOHIKO TAMURA Triana Hertiani Trisanti Anindyawati, Trisanti Untari, Febriana WIBOWO MANGUNWARDOYO Yantyati Widyastuti YOPI YOPI YULIN LESTARI