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Journal : Journal of Applied Health Management and Technology

Detection of rpoB Gene Mutations in Mycobacterium Tuberculosis widodo widodo; Ahmad Riadi; Devi Etevia Purlinda; Lilik Setyowatiningsih; roni roniafriansya
Journal of Applied Health Management and Technology Vol 5, No 3 (2023): July 2023
Publisher : Politeknik Kesehatan Kementerian Kesehatan Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v5i3.10093

Abstract

Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik terhadap resistensi perlu dikembangkan salah satunya methode molekuler berbasis PCR penentu mutasi gen yang disebakan resistensi obat salah satu target obat yang digunakan adalah rpoB, pengunaan Primer rpoB sebagai penanda terjadinya mutasi diharapkan mampu memberikan dampak sebagai alternatif deteksi cepat secara molekuler. Panjang pita rpoB 318 bp cocok untuk kelompok Mycobacterium tuberculosis diperoleh hasil menunjukan pada kodon 509 perubagan AGC menjadi AAT terjadi perubahan asam amino dari serin menjadi Apargarin, perubahan nukleotida  CAG menjadi CCG pada kodon 510 mengubah asam amino Glutamin menjadi prolin, kodon 514 perubahan nukleotida TTC menjadi TTG mengubah asam amino Phenylalanine menjadi leucine, kodon 515 terjadi perubahan nukleotida ATG menjadi GTG perubahan ini menyebakan perubahan asam amino Methionine menjadi Valine  . perubahan tersebut menyebabkan terjadinya resistensi terhadap obat Rifampicin
ANTIBODY SCREENING EXAMINATION ON VOLUNTARY BLOOD DONORS AT BLOOD DONATION UNITS BANYUMAS REGENCY IN 2023 ETIVIA, DEVI; Riadi, Ahmad; widodo, widodo; Lestari, Sri
Journal of Applied Health Management and Technology Vol 6, No 1 (2024): April 2024
Publisher : Politeknik Kesehatan Kementerian Kesehatan Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v6i1.11364

Abstract

Blood donation is a humanitarian activity where someone donates blood voluntarily. Blood Donation Units are required to provide safe and quality blood products to avoid transfusion reactions. One effort that can be made is to carry out pre-transfusion examinations on donor blood, one of which is an antibody screening examination. The aim of the antibody screening examination is to detect the presence or absence of clinically significant irregular antibodies in both the patient and donor. The aim of this study was to determine the blood type, gender and age of antibody screening in voluntary blood donors at the Blood Donation Unit. This type of research is descriptive using observational methods which was carried out in March 2023 at the Banyumas Regency Blood Donation Unit. Samples were taken using a total sampling technique, namely all voluntary blood donors who underwent an antibody screening test. Data collection was carried out using the qwalist diagast tool and laboratory archives which were accessed via the SIMDONDAR system. The results of the antibody screening research from a total of 3198 voluntary blood donors showed that the highest results were in blood type O, namely 1236 (39%) donors, in men, namely 2196 (69%), and in the elderly (26).-60 years).namely 1531 (48%) donors
ANTIBODY SCREENING EXAMINATION ON VOLUNTARY BLOOD DONORS AT BLOOD DONATION UNITS BANYUMAS REGENCY IN 2023 ETIVIA, DEVI; Riadi, Ahmad; widodo, widodo; Lestari, Sri
Journal of Applied Health Management and Technology Vol. 6 No. 1 (2024): April 2024
Publisher : Postgraduate Program , Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v6i1.11364

Abstract

Blood donation is a humanitarian activity where someone donates blood voluntarily. Blood Donation Units are required to provide safe and quality blood products to avoid transfusion reactions. One effort that can be made is to carry out pre-transfusion examinations on donor blood, one of which is an antibody screening examination. The aim of the antibody screening examination is to detect the presence or absence of clinically significant irregular antibodies in both the patient and donor. The aim of this study was to determine the blood type, gender and age of antibody screening in voluntary blood donors at the Blood Donation Unit. This type of research is descriptive using observational methods which was carried out in March 2023 at the Banyumas Regency Blood Donation Unit. Samples were taken using a total sampling technique, namely all voluntary blood donors who underwent an antibody screening test. Data collection was carried out using the qwalist diagast tool and laboratory archives which were accessed via the SIMDONDAR system. The results of the antibody screening research from a total of 3198 voluntary blood donors showed that the highest results were in blood type O, namely 1236 (39%) donors, in men, namely 2196 (69%), and in the elderly (26).-60 years).namely 1531 (48%) donors
Detection of rpoB Gene Mutations in Mycobacterium Tuberculosis widodo, widodo; Riadi, Ahmad; Etevia Purlinda, Devi; Setyowatiningsih, Lilik; roniafriansya, roni
Journal of Applied Health Management and Technology Vol. 5 No. 3 (2023): July 2023
Publisher : Postgraduate Program , Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v5i3.10093

Abstract

Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik terhadap resistensi perlu dikembangkan salah satunya methode molekuler berbasis PCR penentu mutasi gen yang disebakan resistensi obat salah satu target obat yang digunakan adalah rpoB, pengunaan Primer rpoB sebagai penanda terjadinya mutasi diharapkan mampu memberikan dampak sebagai alternatif deteksi cepat secara molekuler. Panjang pita rpoB 318 bp cocok untuk kelompok Mycobacterium tuberculosis diperoleh hasil menunjukan pada kodon 509 perubagan AGC menjadi AAT terjadi perubahan asam amino dari serin menjadi Apargarin, perubahan nukleotida  CAG menjadi CCG pada kodon 510 mengubah asam amino Glutamin menjadi prolin, kodon 514 perubahan nukleotida TTC menjadi TTG mengubah asam amino Phenylalanine menjadi leucine, kodon 515 terjadi perubahan nukleotida ATG menjadi GTG perubahan ini menyebakan perubahan asam amino Methionine menjadi Valine  . perubahan tersebut menyebabkan terjadinya resistensi terhadap obat Rifampicin