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All Journal JURNAL PEMULIAAN TANAMAN HUTAN Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology Agrin : Jurnal Penelitian Pertanian Journal of Tropical Crop Science Menara Perkebunan Nusantara Science and Technology Proceedings
Riza Arief Putranto
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Journal of Tropical Crop Science

 1 
Characterization of a Drought-Inducible Dehydrin Promoter from Sugarcane (Saccharum officinarum L.) in Tobacco (Nicotiana tabacum L.) Iskandar, Hayati Minarsih; Suhandono, Sonny; Pambudi, Jembar; Kristianti, Tati; Putranto, Riza Arief; Mose, Windi; Sustiprijatno, Sustiprijatno
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (910.185 KB) | DOI: 10.29244/jtcs.7.01.28-36

Abstract

Dehydrin (DHN) is known to play an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). Previous research reported the increased expression of DHN in sugarcane stems exposed to drought stress for 15 days which may be controlled by its corresponding stress inducible promoter. The DHN promoter was succesfully isolated from sugarcane variety PSJT 941 (Pr-1DHNSo) and was cloned to pBI121 expression vector fused to a β-glucuronidase (GUS) reporter gene. The aim of this research was the functional testing of the Pr-1DHNSo promoter through transformation into tobacco plant treated with in vitro drought stress. Genetic transformation of Pr-1DHNSo construct was conducted by Agrobacterium tumefaciens. The transformed tobacco was then subjected to drought stress treatment using 40% PEG 6000 for five sequential incubations (0, 12, 24, 48 and 72 hours). The GUS assay reveal that the transformed tobacco treated with drought stress showed a blue color denoting GUS activity in leaf, stem and root tissues and this expression increased along with the length of the drought treatment. The analysis of gusA gene using real time-qPCR normalized to the L25 reference gene also showed that the expression increased in line with the length of time of drought stress. The results presented in this study indicated that the Pr-1DHNSo promoter from sugarcane was expressed and induced by drought stress treatment in tobacco.
Co-Authors . KUSWANHADI . NUHAIMI-HARIS . PRIYONO . SISWANTO . SUMARYONO Afandi, Dadang Agustin Sri MULYATNI Akhirta Atikana Aksa, Annisa Aulia Alfero Putra Iryanto Anggia Prasetyoputri Annisa A Aksa Asmini Budiani Asmini BUDIANI Asmini Budiani Asmini Budiani Asmini Budiani Asmini BUDIANI1 Barahima Abbas Christy Dewi Sukma Dini Astika Sari Dini Astika Sari Dini Astika Sari Ernayunita Ernayunita Ernayunita, Ernayunita Galuh Wening PERMATASARI Galuh Wening Permatasari Galuh Wening PERMATASARI Happy WIDIASTUTI Hayati MINARSIH Hayati Minarsih Hayati Minarsih Hayati Minarsih Imam Bagus Nugroho Imron Riyadi Imron Riyadi Imron RIYADI Imron Riyadi Indra Syahputra Inez Palupi Irfan MARTIANSYAH Irfan Martiansyah Iskandar, Hayati Minarsih Jembar PAMBUDI Julie LECLERCQ Lailia Zubaidah Larasati D Mahardhika Lutfi Anggadhania Mardhika, Larasati Dena MARTIANSYAH, Irfan Masna Maya Sinta Masna Maya Sinta Mose, Windi Muhammad Farrel Ewaldo Mukmin, Restu Prasetya Nurul Khumaida Nurul Khumaida Pambudi, Jembar Pascal MONTORO Pascal MONTORO Pascal MONTORO PERMATASARI, Galuh Wening pri yono Radite TISTAMA Ratih Asmana Ningrum Rizka Tamania Saptari Rizka Tamania SAPTARI Rizka Tamania Saptari Rokhana Faizah Rokhana Faizah Sapto Nugroho Hadi Sapto Nugroho Hadi Sari, Dini Astika Sekar WOELAN Sri Wening Sri Wening Sudarsono Sudarsono Suhandono, Sonny Sustiprijatno Sustiprijatno, Sustiprijatno TATI KRISTIANTI Turhadi Turhadi Vivi Restu Raharti Y.M.M Anita Nugraheni Yuli Setiawati Yuli Setiawati Yuli Setiawati, Yuli