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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH Biosfer: Jurnal Tadris Biologi Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Planta Tropika Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati IJEMS (Indonesian Journal of Environmental Management and Sustainability) Biosaintifika: Journal of Biology & Biology Education Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Yulita Nurchayati
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Reni Indriani; Erma Prihastanti; Rini Budihastuti; Yulita Nurchayati
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
KANDUNGAN ASAM ASKORBAT PADA KULTUR KALUS ROSELA (hibiscus sabdariffa L.) DENGAN VARIASI KONSENTRASI SUKROSA DALAM MEDIA MS Yulita Nurchayati; Fathiyah Afiah R
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.858 KB) | DOI: 10.22146/tradmedj.8072

Abstract

Ascorbic acid is an antioxidant which is found in rosella flower. Its production through conventional method needs long time and wide field, so an alternative method through development of callus invitro is conducted. Sucrose is a main carbon source in MS medium and this sugar plays role as precursor for the formation of ascorbic acid. This study was conducted to determine the effect of sucrose concentration on callus formation and production of ascorbic acid. Sucrose was added to the media at the concentration  of 20 g/L, 30 g/L, 40 g/L and 50 g/L. The culture was incubated for 42 days with twice subculture every 10 days.  Analysis of ascorbic acid was perfomed using iodometry. The results showed that the callus was grow better in sucrose of 20 g/L while the ascorbic acid production was better in sucrose 50 g/L. 
Kandungan klorofil dan karotenoid Kacang Tunggak (Vigna unguiculata (L.) Walp.) pada umur tanaman yang berbeda Ika Susanti Hendriyani; Yulita Nurchayati; Nintya Setiari
Jurnal Biologi Tropika Vol.1, No. 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.952 KB) | DOI: 10.14710/jbt.1.2.38-43

Abstract

Chlorophyll is a pigment of photosynthesis which is also efficacious as a cure for brain, lung, and oral cancer, and can be used as a disinfectant, antibiotic and dietary supplement, while carotenoids are useful as antioxidants. The content of the two pigments is strongly influenced by the age of the plant, especially for vegetable crops used in leaves such as cowpea (Vigna unguiculata (L.) Walp.). The purpose of this research is to know the pattern of chlorophyll content and carotenoid of cowpea leaf during the growth phase and to know the age of the right plant for harvesting cowpea so that the highest chlorophyll and carotenoid content is obtained. Cowpea seeds obtained from seeds are added then planted in pots containing ready-made planting media in the greenhouse. Chlorophyll and carotenoid analyzes were performed at different plant ages from 1 to 11 weeks. The research design used is Completely Randomized Design. Data were analyzed using variance analysis and continued with Duncan test at 95% significance level. The results showed that the highest chlorophyll and carotenoid content was produced by the cowpea leaf in the vegetative phase or before the flowering time. The highest chlorophyll content was obtained at 4WAP (weeks after planting) of age crop, while the highest carotenoid content was obtained in 3WAP.  
Pertumbuhan vegetatif anggrek Dendrobium stratiotes Rchb.f. setelah pemberian monosodium glutamat dan pupuk “Hortech” Nintya Setiari; Yulita Nurchayati
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.485 KB) | DOI: 10.14710/jbt.2.1.16-20

Abstract

Fertilizers are needed to encourage the growth of orchids because their growth is known to be very slow. Monosodium glutamate (MSG) is a crystalline salt containing sodium and glutamate which can stimulate plant growth. Therefore in this study, the application of MSG and fertilizer was carried out to promote the growth of D. stratiotesorchids. The application of MSG and fertilizer was given in a completely randomized design with a factorial pattern consisting of MSG concentration factors (0, 0.5 and 1%) and fertilizing factors (not fertilized and fertilized). There are six treatments and three replications per treatments. The parameters observed increased in leaf length (cm), the morphological response of orchid plants in the form of new shoot formation, new root formation, and bulb enlargement. Results of research and test Analysis of Variance (ANOVA) showed no interaction between MSG and fertilizer in influencing the length leaf orchid D. stratiotes. The length of leaves is highest in orchids by MSG 1% without fertilizer, while the formation of new shoots and bulb enlargement occurred in orchid plants which were given 0.5 and 1% MSG without fertilizer application. New root formation occurs in plants given MSG and fertilizer. The conclusion is the application of 1% MSG without fertilizer can promote the growth of. D. stratiotesorchids.
Pengaruh pupuk daun Gandasil D terhadap pertumbuhan, kandungan klorofil dan karotenoid tanaman bayam merah (Alternanthera amoena Voss.) Fetryani Soni Manurung; Yulita Nurchayati; Nintya Setiari
Jurnal Biologi Tropika Vol. 3, No. 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.586 KB) | DOI: 10.14710/jbt.1.1.24-32

Abstract

Red spinach is (Alternanthera amoena Voss.) a vegetable that contains vitamins (vitamin A, C, and E) and minerals.Yield optimization red amaranth cultivation can be done with additional supplementary fertilizer like fertilizer leaves Gandasil D. The purpose of this study are to examine the effect of Gandasil D fertilizer on vegetative growth of plants and determine the optimal concentration of Gandasil D fertilizer on the chlorophyll and carotenoid content of the leaves. The experiment was carried out by germinating red spinach seeds, transferring 14 -days-old seedlings into pots containing planting media and basic fertilizers.  Seedlings at 14-days-old were then given Gandasil D fertilizer by spraying on the surface of the leaves once a week until the age 32–days-old. The study was conducted with a CRD with a single factor with 4 treatments of Gandasil D concentration, namely 0g/L (control), 1g/L, 2g/L, and 3g/L with 5 replications.  Parameters observed that the number of leaves, plant height, root length, wet weight, dry weight, chlorophyll, and carotenoid content.  Data were analyzed by ANOVA followed by Duncan’ test at the 95% significance level. The results showed that the application of Gandasil D significantly affected the number of leaves, plant height, and fresh weight of the plant.  Spraying with Gandasil D did not show a significant effect on root length, plant dry weight, and chlorophyll and red spinach carotenoid content.  Gandasil D fertilizer at a concentration of  3g/L produces the most optimal growth of red spinach plants.
Karakterisasi morfologi dan fisiologi dari tiga varietas kentang (Solanum tuberosum L. ) di Kabupaten Magelang Jawa Tengah Yulita Nurchayati; Nintya Setiari; Nita Kumalasari Dewi; Fella Suffah Meinaswati
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3443.385 KB) | DOI: 10.14710/niche.2.2.38-45

Abstract

Pengaruh ekstrak daun suren (Toona sureni Merr.) terhadap produksi buah cabai rawit yang diserang penyakit antraknosa Fiva Andriyani; Yulita Nurchayati; Sri Haryanti
NICHE Journal of Tropical Biology Vol. 3, No. 2, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.2.89-98

Abstract

Penggunaan Kinetin, Asam Naftalen Asetat, dan Benzil Adenin dalam Induksi Kalus Kecubung (Datura metel L.) Secara In Vitro Yulita Nurchayati; Santosa Santosa; Laurentius H Nugroho; Ari Indrianto
Buletin Anatomi dan Fisiologi Volume 3, Nomor 1, Tahun 2018
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.3.1.2018.105-109

Abstract

Kecubung (Datura metel L.) merupakan tumbuhan penghasil hyosciamin dan skopolamin, yang berkasiat sebagai anticholergic dan spasmolytic. Kedua metabolit dapat ditingkatkan produksinya dari kultur kalus. Induksi  kalus dari daun kecubung dilakukan dengan menggunakan aplikasi zat pengatur tumbuh (ZPT). Tujuan dari penelitian ini adalah untuk mempelajari respon dan perkembangan daun kecubung terhadap beberapa macam hormon dan untuk menseleksi kalus yang terbentuk secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 6 perlakuan ZPT dalam medium Murashige & Skoog (MS) yaitu tanpa hormon, Kinetin tunggal, NAA tunggal, kombinasi Kinetin dan NAA, BA tunggal serta kombinasi BA dan NAA.  Daun yang telah disterilkan ditumbuhkan dalam medium MS dengan penambahan sukrosa 3% dan beberapa ZPT perlakuan dan diberikan 5 ulangan.   Kalus yang terbentuk disubkultur dalam medium yang sama formulasinya untuk mengoptimalkan pertumbuhan. Hasil menunjukkan bahwa kalus dapat terbentuk pada semua medium yang diujikan. Kalus yang remah diperoleh dari medium MS dengan ZPT kombinasi kinetin 3x10-5 M dan NAA 10-5 M. Respon kalus yang berbeda diperoleh dari medium dengan penambahan BA tanpa NAA. Penambahan BA tunggal 6x10-6 M dalam medium MS menginduksi kalus yang embrionik. Kata kunci : induksi kalus; zat pengatur tumbuh; kalus remah; kalus embrionik 
Kandungan Klorofil dan Karotenoid Daun Salam (Syzigium polyanthum (Wight) Walp.) pada Umur yang Berbeda Abdul Khafid; Yulita Nurchayati; Sri Widodo Agung Suedy
Buletin Anatomi dan Fisiologi Volume 6, Nomor 1, Tahun 2021
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.6.1.2021.74-80

Abstract

Pohon salam (Syzygium polyanthum (Wight) Walp.) merupakan salah satu tumbuhan yang daunnya sering dimanfaatkan oleh masyarakat di berbagai daerah di Indonesia sebagai penyedap rasa dalam masakan dan obat-obatan tradisional. Daun salam umumnya dimanfaatkan dalam kondisi segar. Pemanfaatan daun segar tersebut dilakukan berdasar umur daun, yang berkaitan dengan kandungan bahan aktifnya. Penelitian ini bertujuan untuk mengetahui kandungan pigmen klorofil dan karotenoid daun salam pada umur yang berbeda. Penelitian menggunakan Rancangan Acak Lengkap, sampel daun diambil dari urutan daun yang berbeda berdasarkan letak duduk daun yaitu urut dari daun paling ujung (muda) merupakan daun ke-1, ke-3, ke-5, dan daun ke-7. Ulangan sebanyak 3 kali dari tangkai pohon yang berbeda. Parameter yang diamati adalah kandungan klorofil-a, klorofil-b, klorofil total dan karotenoid yang diukur secara spektrofotometri. Data dianalisis menggunakan ANOVA dilanjutkan dengan DMRT. Hasil penelitian menunjukkan bahwa Kandungan pigmen klorofil dan karotenoid meningkat seiring pertambahan umur daun. Kandungan klorofil-a tertinggi pada daun ke-3, sementara klorofil-b,klorofil total dan karotenoid tertinggi pada daun ke-5.
Efek Naungan dan Umur Tanaman Terhadap Pertumbuhan dan Profil Metabolit Bunga Krisan (Chrysanthemum sp.) Puji Nur Hana; Yulita Nurchayati; Rini Budihastuti
Buletin Anatomi dan Fisiologi Volume 5, Nomor 1, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.1.2020.8-17

Abstract

Pertumbuhan dan profil metabolit tanaman krisan (Chrysathemum sp.) dipengaruhi umur fisiologi dan cahaya. Tujuan penelitian ini adalah mengetahui pengaruh perlakuan kombinasi naungan dan umur fisiologi tanaman terhadap pertumbuhan dan profil metabolit bunga krisan, serta mengetahui senyawa spesifik dari profil metabolit bunga krisan. Penelitian dilaksanakan di Kebun Krisan Bandungan Jawa Tengah pada bulan Maret hingga Oktober 2018. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal 4 perlakuan dengan 10 kali ulangan, yaitu kombinasi perlakuan naungan 0% umur fisiologi tanaman 80 HST (P0 U80) dan 125 HST (P0 U125), naungan 75% umur fisiologi tanaman 80 HST (P1 U80) dan 125 HST (P1 U125). Perlakuan P0 U125 menghasilkan pertumbuhan tanaman paling tinggi yaitu 131,40 cm, dan diameter bunga yang lebih besar yaitu 6,38 cm. Perlakuan P1 U125 menghasilkan jumlah bunga terbanyak yaitu 21,40 bunga. Perlakuan naungan 0% menyebabkan waktu inisiasi bunga lebih cepat 104,1 HST dibanding naungan 75% 112,8 HST. Kesimpulkan penelitian ini adalah perlakuan kombinasi berpengaruh terhadap tinggi tanaman dan jumlah bunga pada krisan. Pertumbuhan tanaman krisan tertinggi terdapat pada perlakuan P0 U125, jumlah bunga tertinggi pada perlakuan P1 U125. Terdapat senyawa spesifik etil linoleat pada perlakuan P1 U125, merupakan kelompok senyawa asam lemak pembentuk pyrethrin yang berpotensi sebagai insektisida nabati. Kata kunci: bioinsektisda, metabolit sekunder, intensitas cahaya, umur tanaman
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Diah Nurmaningrum Ditasya Putri, Novika Einstivina Nuryandani Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur Hartati, Puji I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Indriani, Reni Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Misrofah, Sofatu Misrofah, Sofatun Mochammad Syaifuddin Muhammad Syaiful Anam Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Noveira, Ameviana Fitri Nur Cahyani, Evi Prameswari, Ratna Apriliani Puji Nur Hana Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Santosa Santosa Santosa Santosa Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utama, Rafii Satria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Zahrotunnisa, Alfi Zakiyah, Aisyah