Article Per Year (5 Year)
p-Index From 2021 - 2026
4.704
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH Biology, Medicine, & Natural Product Chemistry Planta Tropika Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Yulita Nurchayati
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health

Published : 61 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 61 Documents
Search

 1   2   3   4   5 
Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Reni Indriani; Erma Prihastanti; Rini Budihastuti; Yulita Nurchayati
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
Pertumbuhan Plantlet Anggrek Phalaenopsis amabilis (L.) Blume pada Tahap Subkultur dengan Perlakuan Jenis Media dan Konsentrasi Pepton Berbeda Anggi Krisdianto; Endang Saptiningsih; Yulita Nurchayati; Nintya Setiari
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p06

Abstract

Phalaenopsis amabilis is an orchid native of Indonesia that included in the appendix II category and one of the flowers included in the national and international flower trade, so that needs to be used in vitro propagation. The type of media used in the subculture determines the results of plantlet growth. Media used include New Phalaenopsis (NP), Vacint and Went (VW), and Murashige and Skoog (MS). Pepton as supplement added to the media. This study aims to analyze the different media types and peptone concentrations during the subculture. The method used is a plantlet subculture to a media bottle. This study uses a completely randomized design with 4x3 factorial patterns and 3 replications. Data were analyzed with ANOVA at 95% significance. Plantlet P. amabilis was subcultured in MS, VW and NP media with peptone (0; 1; 2; 3 g/L). Growth was observed for 2 months. The parameters observed were the number, length, width, appearance of leaves; number, length, appearance of the root. ANOVA test showed that the two treatments did not have interactions with all parameters, except leaf length. The results showed that the treatment of media type and peptone concentration significantly affected all parameters. NP media and peptone concentration of 3 g/L are the best media and concentrations for the number of leaves (5.67; 5.89), leaf appearance (19.83; 20.22 days ); leaf width (0.42; 0.47 cm), leaf length (1.47 cm); root length (2.83; 2.86 cm), number of roots (2.33; 2.56), and root appearance (28; 27.22 days). The combination of NP media with pepton concentration of 3 g/L is the best combination for the growth of P. amabilis plantlets at the subculture stage.
Produksi Flavonoid Pada Kalus Tomat (Lycopersicon esculentum Mill.) Secara In Vitro Dalam Medium MS Dengan Konsentrasi Sukrosa Yang Berbeda Rizqi Fadlia Julianti; Yulita Nurchayati; Nintya Setiari
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p15

Abstract

Flavonoids are one of the compounds contained in tomatoes and act as antioxidants. Its production can be increased using callus culture. Factors that influence it is sucrose in media culture. Sucrose can be used as a carbon source and have the ability to increase the production of secondary metabolites. The aim of this research was to examine the effect of sucrose production which can increase the flavonoids from tomato plants. The explants used in the study were cotyledons from the aseptic germinated of the Permata F1 tomato variety. The method used was callus induction in MS media. Pieces of cotyledons from sprouts aged 7 DAP were grown in MS medium with the addition of 1 mg/L NAA and 1 mg/L BAP hormone and sucrose according to treatment for 49 days. The research design used a completely randomized design (CRD) with a single factor, namely 3 treatments of sucrose concentration : 20 g/L, 30 g/L, and 40 g/L with 6 replications. Qualitative and quantitative analyzes of flavonoid content were performed using a UV-vis spectrophotometer. The results showed that the concentration of sucrose had an effect on increasing the content and production of flavonoids in callus tomatoes. The addition of sucrose in MS media resulted in a compact callus texture and the fastest callus initiation time was reached at a concentration of 40 g/L. Sucrose concentration from 20-40 g/L increased the production of flavonoids. Sucrose concentration of 40 g/L is the best treatment to increase the growth and production of tomato callus in vitro. Keywords : cotyledons, tomato, flavonoids, callus, sucrose
Pertumbuhan Kantong Semar (Nepenthes mirabilis (Lour.) Druce) Pada Berbagai Media Tanam Rina Budi Astuti; Sri Widodo Agung Suedy; Yulita Nurchayati; Nintya Setiari
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p06

Abstract

Nepenthes mirabilis (Lour.) is a unique plant with a pitcher at the tip of the leaf. This species belongs to a rare category so needed to be preserved. One of the conservation efforts are the selection of the composition growing media for cultivation. This study aimed to found alternative planting media that can support the growth of Nepenthes other than sphagnum moss media. The research was conducted at the green house in Ngablak Village, Ngablak District, Magelang Regency from August to November 2020. This study used a Completely Randomised Design (CRD) with 4 treatments, sphagnum moss, roasted husk, roasted husk with cocopeat (3:1), sphagnum moss with cocopeat (3:1) given of 4 replications. Research parameter include, number of leaves, leaf length, leaf width, and plant height measured at 12 Weeks After Planting (WAP). Observation data were analysis by Analysist of Varians (ANOVA) and Duncan Multiple Range Test (DMRT) on a confidence level of 95%. The result showed that all treatment had a significant effect on the number of leaves, leaf length, leaf width, and plant height. The best planting medium was sphagnum moss with the time of appearance of the leaves (2,39 week), the highest number of leaves (11,25 sheet), and plant height (1,6 cm). the mixture media of roasted husk and cocopeat (3:1) produced growth that wsn’t different from sphagnum moss, so it was recommended to be a cheaper alternative media. Keyword: Nepenthes mirabilis, sphagnum moss, roasted husks, cocopeat
Pertumbuhan planlet anggrek Cymbidium bicolor Lindl. pada tahap subkultur dengan variasi media Fakhri Fadhlurrohman Pratama; Nintya Setiari; Yulita Nurchayati
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p08

Abstract

Cymbidium bicolor is one of the orchids in Indonesia which has beautiful and longlasting flowers, so it has potential to become an ornamental plant and has economic value. The method of propagation generally is tissue culture. One of the stages in plant tissue culture is subculture. The variation of media used at the subculture stage make an effect for growth of plantlet. Media usually can be used include Murashige and Skoog (MS), Vacin and Went (VW), and New Phalaenopsis (NP). This study aims is to analyze the variation of media during the subculture. The method used was tissue culture in subculture with media as treatment. Cymbidium bicolor planlets were subcultured into MS, VW, and NP and observed for 2 months. The parameters observed were the leaf emergence, the number of leafs, leaf length, leaf width, the number of roots, root length, and the number of shoots. This study used a completely randomized design with single factor and 15 replicates. Data were analyzed by ANOVA with 95% significance. Treatment of variation of media significantly affected all parameters. Murashige and Skoog (MS) medium was the best medium for leaf emergence (11 days), the number of leafs (4,92), leaf length (1,56 cm), leaf width (0,33 cm), the number of roots (2,67), root length (2,73 cm), and the number of shoots (4,00). Murashige and Skoog (MS) medium was the best medium and concentrations for C. bicolor growth at the subculture stage.
KANDUNGAN ASAM ASKORBAT PADA KULTUR KALUS ROSELA (hibiscus sabdariffa L.) DENGAN VARIASI KONSENTRASI SUKROSA DALAM MEDIA MS Yulita Nurchayati; Fathiyah Afiah R
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.858 KB) | DOI: 10.22146/tradmedj.8072

Abstract

Ascorbic acid is an antioxidant which is found in rosella flower. Its production through conventional method needs long time and wide field, so an alternative method through development of callus invitro is conducted. Sucrose is a main carbon source in MS medium and this sugar plays role as precursor for the formation of ascorbic acid. This study was conducted to determine the effect of sucrose concentration on callus formation and production of ascorbic acid. Sucrose was added to the media at the concentration  of 20 g/L, 30 g/L, 40 g/L and 50 g/L. The culture was incubated for 42 days with twice subculture every 10 days.  Analysis of ascorbic acid was perfomed using iodometry. The results showed that the callus was grow better in sucrose of 20 g/L while the ascorbic acid production was better in sucrose 50 g/L. 
Kandungan klorofil dan karotenoid Kacang Tunggak (Vigna unguiculata (L.) Walp.) pada umur tanaman yang berbeda Ika Susanti Hendriyani; Yulita Nurchayati; Nintya Setiari
Jurnal Biologi Tropika Vol.1, No. 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.952 KB) | DOI: 10.14710/jbt.1.2.38-43

Abstract

Chlorophyll is a pigment of photosynthesis which is also efficacious as a cure for brain, lung, and oral cancer, and can be used as a disinfectant, antibiotic and dietary supplement, while carotenoids are useful as antioxidants. The content of the two pigments is strongly influenced by the age of the plant, especially for vegetable crops used in leaves such as cowpea (Vigna unguiculata (L.) Walp.). The purpose of this research is to know the pattern of chlorophyll content and carotenoid of cowpea leaf during the growth phase and to know the age of the right plant for harvesting cowpea so that the highest chlorophyll and carotenoid content is obtained. Cowpea seeds obtained from seeds are added then planted in pots containing ready-made planting media in the greenhouse. Chlorophyll and carotenoid analyzes were performed at different plant ages from 1 to 11 weeks. The research design used is Completely Randomized Design. Data were analyzed using variance analysis and continued with Duncan test at 95% significance level. The results showed that the highest chlorophyll and carotenoid content was produced by the cowpea leaf in the vegetative phase or before the flowering time. The highest chlorophyll content was obtained at 4WAP (weeks after planting) of age crop, while the highest carotenoid content was obtained in 3WAP.  
Pertumbuhan vegetatif anggrek Dendrobium stratiotes Rchb.f. setelah pemberian monosodium glutamat dan pupuk “Hortech” Nintya Setiari; Yulita Nurchayati
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.485 KB) | DOI: 10.14710/jbt.2.1.16-20

Abstract

Fertilizers are needed to encourage the growth of orchids because their growth is known to be very slow. Monosodium glutamate (MSG) is a crystalline salt containing sodium and glutamate which can stimulate plant growth. Therefore in this study, the application of MSG and fertilizer was carried out to promote the growth of D. stratiotesorchids. The application of MSG and fertilizer was given in a completely randomized design with a factorial pattern consisting of MSG concentration factors (0, 0.5 and 1%) and fertilizing factors (not fertilized and fertilized). There are six treatments and three replications per treatments. The parameters observed increased in leaf length (cm), the morphological response of orchid plants in the form of new shoot formation, new root formation, and bulb enlargement. Results of research and test Analysis of Variance (ANOVA) showed no interaction between MSG and fertilizer in influencing the length leaf orchid D. stratiotes. The length of leaves is highest in orchids by MSG 1% without fertilizer, while the formation of new shoots and bulb enlargement occurred in orchid plants which were given 0.5 and 1% MSG without fertilizer application. New root formation occurs in plants given MSG and fertilizer. The conclusion is the application of 1% MSG without fertilizer can promote the growth of. D. stratiotesorchids.
Pengaruh pupuk daun Gandasil D terhadap pertumbuhan, kandungan klorofil dan karotenoid tanaman bayam merah (Alternanthera amoena Voss.) Fetryani Soni Manurung; Yulita Nurchayati; Nintya Setiari
Jurnal Biologi Tropika Vol. 3, No. 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.586 KB) | DOI: 10.14710/jbt.1.1.24-32

Abstract

Red spinach is (Alternanthera amoena Voss.) a vegetable that contains vitamins (vitamin A, C, and E) and minerals.Yield optimization red amaranth cultivation can be done with additional supplementary fertilizer like fertilizer leaves Gandasil D. The purpose of this study are to examine the effect of Gandasil D fertilizer on vegetative growth of plants and determine the optimal concentration of Gandasil D fertilizer on the chlorophyll and carotenoid content of the leaves. The experiment was carried out by germinating red spinach seeds, transferring 14 -days-old seedlings into pots containing planting media and basic fertilizers.  Seedlings at 14-days-old were then given Gandasil D fertilizer by spraying on the surface of the leaves once a week until the age 32–days-old. The study was conducted with a CRD with a single factor with 4 treatments of Gandasil D concentration, namely 0g/L (control), 1g/L, 2g/L, and 3g/L with 5 replications.  Parameters observed that the number of leaves, plant height, root length, wet weight, dry weight, chlorophyll, and carotenoid content.  Data were analyzed by ANOVA followed by Duncan’ test at the 95% significance level. The results showed that the application of Gandasil D significantly affected the number of leaves, plant height, and fresh weight of the plant.  Spraying with Gandasil D did not show a significant effect on root length, plant dry weight, and chlorophyll and red spinach carotenoid content.  Gandasil D fertilizer at a concentration of  3g/L produces the most optimal growth of red spinach plants.
Karakterisasi morfologi dan fisiologi dari tiga varietas kentang (Solanum tuberosum L. ) di Kabupaten Magelang Jawa Tengah Yulita Nurchayati; Nintya Setiari; Nita Kumalasari Dewi; Fella Suffah Meinaswati
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3443.385 KB) | DOI: 10.14710/niche.2.2.38-45

Abstract

Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Diah Nurmaningrum Ditasya Putri, Novika Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur Hartati, Puji I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Indriani, Reni Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Latif, Fahmi Nur Aida Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Misrofah, Sofatu Misrofah, Sofatun Mochammad Syaifuddin Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Nur Cahyani, Evi Prameswari, Ratna Apriliani Puji Nur Hana Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Safinah, Safinah Santosa Santosa Santosa Santosa Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Zakiyah, Aisyah