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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH Biology, Medicine, & Natural Product Chemistry Planta Tropika Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Yulita Nurchayati
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health

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Peningkatan Pertumbuhan Anggrek Phalaenopsis amabilis (L.) Blume secara In Vivo setelah Pemberian Thidiazuron (TDZ) Nur Cahyani, Evi; Nintya Setiari; Yulita Nurchayati
Jurnal Hortikultura Indonesia (JHI) Vol. 16 No. 2 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.2.92-99

Abstract

Phalaenopsis amabilis (L.) Blume merupakan salah satu jenis anggrek yang banyak dimanfaatkan sebagai tanaman hias dan sebagai spesies induk dalam program pemuliaan. Eksploitasi yang berlebihan serta laju pertumbuhan vegetatif yang lambat menjadikan anggrek ini memerlukan upaya konservasi ex-situ melalui penambahan nutrisi dan zat pengatur tumbuh, salah satunya Thidiazuron (TDZ). Penelitian ini bertujuan untuk mengkaji pengaruh serta menentukan konsentrasi TDZ yang optimal terhadap pertumbuhan P. amabilis. Penelitian dilaksanakan pada November 2023 hingga Januari 2024 di Tembalang, Semarang, menggunakan Rancangan Acak Lengkap (RAL) satu faktor, yaitu konsentrasi TDZ (0, 25, dan 50 ppm). Parameter yang diamati meliputi panjang dan lebar daun lama, jumlah dan waktu munculnya daun baru, warna daun, jumlah dan waktu munculnya akar baru, serta densitas stomata. Data dianalisis menggunakan ANOVA dan dilanjutkan dengan uji Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa pemberian TDZ berpengaruh nyata terhadap peningkatan panjang dan lebar daun lama, jumlah dan waktu munculnya daun baru, serta densitas stomata P. amabilis. Thidiazuron (TDZ) juga memengaruhi perubahan warna daun. Konsentrasi TDZ 25 ppm merupakan perlakuan terbaik dalam meningkatkan pertumbuhan daun P. amabilis. Kata kunci: konservasi, sitokinin, vegetatif, zat pengatur tumbuh
Optimization of Arabica Coffee (Coffea arabica L.) Shoot Cutting Growth Using Plastic Bottle Covers and Goat Urine Treatments Safinah, Safinah; Darmanti, Sri; Nurchayati, Yulita
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.867-872

Abstract

Arabica coffee (Coffea arabica L.) is a high-value commodity, yet its productivity in Indonesia remains low due to suboptimal cultivation techniques. One of the main constraints is the limited availability of quality seedlings, which is influenced by propagation methods. Vegetative propagation through shoot cuttings is widely used, but often constrained by low survival and root formation rates. The application of plastic bottle covers and goat urine has the potential to improve propagation success by creating a favorable microclimate and supplying growth regulators and essential nutrients. This study examined the effects of plastic bottle cover types and goat urine concentrations on the growth of C. arabica shoot cuttings. The experiment was arranged in a 3×3 factorial Completely Randomized Design (CRD), with two factors: plastic bottle cover types (no cover, open-cap cover, and closed cover) and goat urine concentrations (0, 100, and 180 mL/L). Observed parameters included shoot length, shoot number, leaf length, leaf number, root length, root number, and root diameter. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s Honestly Significant Difference (HSD) test at a 5% significance level. The results showed a significant interaction between cover type and goat urine concentration. Combining a closed cover with 100 mL/L goat urine produced the highest shoot growth, cutting survival rate, and rooting percentage, while optimal root development was obtained with the closed cover and 180 mL/L goat urine. The most effective treatment was the closed cover combined with 100 mL/L goat urine.
The Effect of Benzyl Amino Purine (BAP) On Potato (Solanum tuberosum L.) Axillary Buds Micropropagation Putri Anugrah Maulidya; Nurchayati, Yulita; Setiari, Nintya
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 01 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss01/480

Abstract

Potato (Solanum tuberosum L.) production in Indonesia increased by 4.21% in 2022 compared to the previous year, but this production is still low by international standards. This is caused by low quality and quantity of potato seeds which is sourced from tubers. It is necessary to modify the in vitro cultivation of potato seed sources by utilising seeds that are often wasted with the addition of cytokinins, such as Benzyl Amino Purine (BAP). The aim of this study was to determine the effect of BAP on potato axillary shoot explants sourced from seeds. The study was conducted using a Completely Randomized Design with one factor, namely BAP concentrations of 0, 1, 2 and 3 ppm with 3 replications. The results showed that there is an influence of BAP on the parameters of shoot growth percentage, leaf and root emergence time, plant height, number of shoots, roots, and leaves of potato axillary buds planlet. The 2 ppm BAP treatment tends to give the best response in increasing the number of shoots with an average of 4.33 shoots, making it effective for producing potato seedlings from axillary buds in vitro.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
Pertumbuhan Anggrek Cymbidium ensifolium (L.) Sw. dengan Pemberian Pupuk Hayati Mikoriza Misrofah, Sofatun; Misrofah, Sofatu; Setiari, Nintya; Nurchayati, Yulita; Suedy, Sri Widodo Agung
Jurnal Hortikultura Indonesia (JHI) Vol. 13 No. 1 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.1.35-42

Abstract

ABSTRAKAnggrek Cymbidium ensifolium adalah anggrek terestrial yang terdaftar di CITES 2020 sebagai spesies hampir terancam punah atau rentan sehingga perlu upaya pelestarian secara ex situ. Salah satu faktor pendukung budidaya anggrek adalah pemberian pupuk. Pupuk hayati mikoriza bersifat ramah lingkungan dan dapat mengurangi dampak negatif pupuk anorganik. Penelitian ini bertujuan untuk mengkaji pengaruh pupuk hayati mikoriza berbagai dosis terhadap pertumbuhan anggrek C. ensifolium. Penelitian ini disusun menggunakan Rancangan Acak Lengkap satu faktor, yaitu pupuk hayati mikoriza (0; 5; 10; 15 g tanaman-1) dengan 6 ulangan. Data dianalisis pada Analysis of Variance dengan taraf kepercayaan 95%. Percobaan dilakukan bulan Oktober 2021 – Februari 2022 di Tembalang, Semarang. Parameter yang diamati berupa waktu muncul tunas, jumlah tunas, waktu muncul daun, jumlah daun, dan persentase infeksi mikoriza. Hasil penelitian menunjukkan bahwa perlakuan pupuk hayati mikoriza berpengaruh signifikan terhadap waktu muncul tunas dan daun, jumlah daun, dan persentase infeksi mikoriza. Pupuk hayati mikoriza sebesar 5 g tanaman-1 merupakan perlakuan optimal untuk meningkatkan waktu muncul tunas dan daun, serta konsentrasi 15 g tanaman-1 optimal meningkatkan jumlah daun dan persentase infeksi mikoriza. Pengaruh pupuk hayati mikoriza ini dapat dikembangkan untuk pelestarian anggrek jenis lain. Kata kunci: anggrek spesies, Cymbidium, biofertilizer, mikoriza
Effect of Cold Storage Duration on the Quality of Super Red (Hylocerus costaricensis) and White (Hylocerus undatus) Dragon Fruits Latif, Fahmi Nur Aida; Nurchayati, Yulita; Hastuti, Endah Dwi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 1 (2025): February 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i1.39-48

Abstract

Postharvest storage is carried out to control the metabolic rate so the quality of fruits can be maintained properly until it reaches the costumers. Cold storage can reduce metabolic reactions, such as respiration and transpiration. However, storing too long in cold temperatures can cause the quality of the fruit to deteriorate. Each type of dragon fruit has different chemical contents that will affect the quality of fruits after storage. This study aims to determine the effect of the type of dragon fruit, cold storage duration, and interaction between the two treatments. Both type of dragon fruit are packed in PP plastic and then stored at 10°C. Observation were made at different storage durations. This study used RAL, with the first being super red and white dragon fruits. The second factor was storage duration of 0, 7, 14, and 21 days. Data were analyzed using ANOVA and continued with DMRT if it had a significant effect. The result of the study showed that the treatment of fruit type had significant effect on antioxidant activity, vitamin C content, and total sugar level. Storage duration treatment had significant effect on antioxidant activity and vitamin C content. Keywords: Antioxidant activity, Cold storage, Dragon fruit, Post-harvest, Vitamin c.
Pengaruh Media MS dan VW Terhadap Pertumbuhan Planlet Anggrek Bulan (Phalaenopsis amabilis L. Blume) Setelah Transplanting Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.971 KB)

Abstract

Anggrek merupakan jenis tanaman hias yang memiliki bunga khas dengan mahkota indah dan warna menarik. Anggrek Bulan (Phalaenopsis amabilis) merupakan bagian dari bunga nasional dan dijuluki “Puspa Pesona”. Ketersediaan Anggrek Bulan di alam mulai berkurang, sehingga diperlukan upaya konservasi. Pembiakan anggrek alam dapat dilakukan dengan teknologi tissue culture atau kultur jaringan biji. Salah satu metode penting dalam kultur jaringan yaitu proses transplanting. Tujuan transplanting agar planlet anggrek tersebut tetap terjaga pertumbuhannya. Penggunaan media tumbuh yang tepatmerupakan salah satu faktor yang menentukan keberhasilan transplanting. Tujuan dari penelitian ini untuk mengetahuipertumbuhan Anggrek Bulan pada dua media berbeda, yaitu MS (Murashige and Skoog) dan VW (Vacin and Went). Metode yang digunakan adalah transplanting planlet Anggrek Bulan umur 1 tahun hasil kultur jaringan dari biji ke media perlakuan MS dan VW tanpa ZPT. Selanjutnya planlet tersebut dipelihara di ruang inkubasi dengan cahaya 1000 lux dan suhu 25℃.Parameter yang diamati yaitu pertambahan jumlah tunas, jumlah daun, panjang daun, lebar daun, dan panjang akar. Pengamatan dilakukan setiap seminggu sekali. Hasil pengamatan menunjukkan bahwa media MS meningkatkan jumlah tunas dan panjang akar, serta mempercepat munculnya tunas baru yaitu satu minggu setelah transplanting. Simpulan dari penelitian ini adalah pertumbuhan planlet cenderung lebih baik di media MS dibanding media VW.
Effect of Ammonium Sulfate on Flower and Pod Development of Soybean [Glycine max (L.) Merr.] Setiana, Devi Vira; Nurchayati, Yulita; Setiari, Nintya
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 1 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.1.17

Abstract

Soybean [Glycine max (L.) Merr.] is a food crop rich in vegetable oil and protein, with increasing demand and economic worth. Increasing soybean production can be accomplished by providing nutrients nitrogen and sulfur, hence increasing the percentage of bloom development into pods. Applying ammonium sulfate at the start of planting can fulfill nitrogen and sulfur fertilizer requirements. The purpose of this study was to examine and determine the ideal concentration of ammonium sulfate for optimal flower and pod formation, as well as the percentage of flower development into pods and leaf sulfur content. Ammonium sulfate was applied in an experimental garden by mixing it into the planting material in polybags under controlled settings. This study employed a single-factor fully randomized design with four doses of ammonium sulfate. The treatments were ammonium sulfate [NH4)2SO4] at various concentrations of 0, 100, 200, and 300 kg/ha administered at the start of planting, with five replications. The research factors were the quantity of flowers, pods, percentage of flower development into pods, and sulfur level in the leaves. The sulfur content of the leaves was determined using an atomic absorption spectrophotometer. Data were analyzed using ANOVA at a significance level of 5%, followed by DMRT if the results were significant. The results revealed that ammonium sulfate 300 kg/ha produced the highest number of flowers, pods, percentage of flower development into pods, and sulfur content in leaves. Keywords: ammonium sulfate, nitrogen, productivity, soybean, sulfur
RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1786

Abstract

Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Diah Nurmaningrum Ditasya Putri, Novika Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur Hartati, Puji I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Indriani, Reni Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Latif, Fahmi Nur Aida Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Misrofah, Sofatu Misrofah, Sofatun Mochammad Syaifuddin Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Nur Cahyani, Evi Prameswari, Ratna Apriliani Puji Nur Hana Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Safinah, Safinah Santosa Santosa Santosa Santosa Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Zakiyah, Aisyah