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All Journal Jurnal Hortikultura Indonesia (JHI) Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH Biosfer: Jurnal Tadris Biologi Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Planta Tropika Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Yulita Nurchayati
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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THE GROWTH OF Dendrobium spectabile (Blume) Miq. ORCHID PLANTLET IN VACIN AND WENT (VW) MEDIUM WITH DIFFERENT CONCENTRATIONS OF COCONUT WATER Zakiyah, Aisyah; Setiari, Nintya; Nurchayati, Yulita
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.222

Abstract

Dendrobium spectabile is an epiphytic orchid that grows slowly and experiences natural exploitation, so it needs to be conserved ex-situ through in vitro culture. One of the essential steps for in vitro culture is subculture to supply the nutrients needed by plantlets. Increasing the growth of these plantlets can be done by adding coconut water. This study aimed to determine the effect of adding coconut water and the optimal concentration of coconut water on the growth of D. spectabile plantlets in VW medium. The method used is growing plantlets in VW medium with different concentrations of coconut water. The research design was completely randomized with a single factor, the concentration of coconut water (0%, 5%, 10%, 15%, 20%). Data were analyzed by ANOVA at a 5% significance level. Plantlet growth was observed for two months. Parameters observed were new shoots, leaves, and roots emergence time; number of new shoots, leaves, and roots; and new leaves length. The addition of coconut water affected the growth of D. spectabile orchid plantlets. Five to ten percent of coconut water is the optimal concentration for shoots and leaves growth of D. spectabile orchid plantlets. Therefore, coconut water can increase the growth of in vitro plantlets.
Peningkatan Pertumbuhan Anggrek Cymbidium ensifolium (L.) Swartz. Setelah Pemberian Pupuk Organik Cair untuk Konservasi Zahrotunnisa, Alfi; Suedy, Sri Widodo Agung; Nurchayati, Yulita; setiari, nintya
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 12 No 4 (2022): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.12.4.579-586

Abstract

Anggrek Cymbidium ensifolium merupakan anggrek terrestrial dengan ciri bunga berwarna kuning corak merah. Keindahan anggrek tersebut menyebabkan masyarakat melakukan eksploitasi yang dapat mengancam kepunahan, sehingga diperlukan upaya pelestarian salah satunya secara ex situ dengan budidaya. Proses budidaya disesuaikan dengan kondisi alamnya yaitu menggunakan bahan alam sebagai pupuk alami atau organik. Bahan alam yang dapat digunakan dalam pembuatan pupuk adalah air kelapa dan air cucian beras. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pupuk organik cair (POC) berbahan air kelapa dan air cucian beras dengan konsentrasi berbeda terhadap pertumbuhan anggrek C. ensifolium. Metode yang digunakan adalah ex situ dengan menanam menggunakan polybag dengan medium tanah: sekam: sekam berambut = 1: 1: 1 dan mikoriza 15 gr/tanaman di kebun percobaan menggunakan rancangan acak lengkap dengan satu faktor berupa konsentrasi POC 0%, 15% dan 30%. Metode analisis data menggunakan ANOVA dan DMRT. Hasil penelitian menunjukkan POC berbahan air kelapa dan air cucian beras berpengaruh meningkatkan pertumbuhan C. ensifolium. Pupuk organik cair 30% merupakan konsentrasi optimal untuk panjang tunas, panjang dan lebar daun. Kebaruan penelitian ini adalah digunakannya kombinasi air kelapa dan air cucian beras sebagai bahan dasar pembuatan pupuk dan pada konsentrasi 30% meningkatkan pertumbuhan anggrek.
Callus Growth and Development of Cayenne Pepper (Capsicum frutescens L.) in Response to Glucose Concentration and Explant Source: Implications for Capsaicin Production via Tissue Culture Syaiful Anam, Muhammad; Nurchayati, Yulita; Setiari, Nintya; Nuryandani, Einstivina
Biosfer: Jurnal Tadris Biologi Vol 17 No 1 (2026): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v17i1.26769

Abstract

Callus culture is a promising biotechnological approach for the sustainable production of secondary metabolites, including capsaicin, without dependence on seasonal factors or large-scale cultivation. This study investigated the effect of glucose concentration on callus growth derived from hypocotyl and cotyledon explants of Capsicum frutescens L. seedlings. A Completely Randomised Design with two factors and three replications was applied using Murashige and Skoog medium supplemented with 2 ppm 2,4-D and 0.5 ppm kinetin, and three glucose concentrations: 30, 40, and 50 g/L. Observed parameters included callus initiation time, colour, texture, and wet weight. Data were analysed using two-way ANOVA and Duncan's Multiple Range Test at P < 0.05. Glucose at 30 g/L produced the fastest initiation and highest wet weight in hypocotyl-derived callus, whereas 40 g/L was optimal for cotyledon-derived callus. These findings provide a basis for optimising tissue culture protocols to support capsaicin production in C. frutescens. Pertumbuhan dan Perkembangan Kalus Cabai Rawit (Capsicum frutescens L.) sebagai Respon terhadap Konsentrasi Glukosa dan Sumber Eksplan: Implikasinya terhadap Produksi Kapsaisin melalui Kultur Jaringan ABSTRAK: Kultur kalus merupakan pendekatan bioteknologi yang menjanjikan untuk produksi metabolit sekunder, termasuk kapsaisin, secara berkelanjutan tanpa bergantung pada faktor musiman maupun budidaya skala luas. Penelitian ini bertujuan mengkaji pengaruh konsentrasi glukosa terhadap pertumbuhan kalus yang berasal dari eksplan hipokotil dan kotiledon Capsicum frutescens L. Rancangan Acak Lengkap dengan dua faktor dan tiga ulangan digunakan pada media Murashige dan Skoog yang diperkaya 2 ppm 2,4-D dan 0,5 ppm kinetin, serta tiga konsentrasi glukosa, yaitu 30, 40, dan 50 g/L. Parameter yang diamati meliputi waktu inisiasi kalus, warna, tekstur, dan berat basah. Data dianalisis menggunakan ANOVA dua arah dan uji Duncan pada taraf P < 0,05. Glukosa 30 g/L menghasilkan inisiasi tercepat dan berat basah tertinggi pada kalus hipokotil, sedangkan 40 g/L optimal untuk kalus kotiledon. Temuan ini menjadi dasar optimasi kultur jaringan untuk mendukung produksi kapsaisin pada C. frutescens.
Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Reni Indriani; Erma Prihastanti; Rini Budihastuti; Yulita Nurchayati
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Diah Nurmaningrum Ditasya Putri, Novika Einstivina Nuryandani Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur Hartati, Puji I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Misrofah, Sofatu Misrofah, Sofatun Mochammad Syaifuddin Muhammad Syaiful Anam Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Nur Cahyani, Evi Puji Nur Hana Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Santosa Santosa Santosa Santosa Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Zahrotunnisa, Alfi Zakiyah, Aisyah