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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH Biology, Medicine, & Natural Product Chemistry Planta Tropika Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Yulita Nurchayati
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health

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In-vitro Callus Development and The Bioactive Compounds of Tomato (Lycopersicon esculentum Mill.) Yulita Nurchayati; Erma Prihastanti; Rini Budihastuti
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21565

Abstract

 AbstractBioactive compounds in tomatoes can be produced through the development of callus culture. This study aimed to investigate callus development and observe bioactive compounds and antioxidant activities in explants and callus. The cotyledon and hypocotyl from the sprouts were induced to form callus on Murashige and Skoog (MS) medium supplemented with NAA 2.5 mg/L combined with kinetin 0.5 mg/L and 2,4 D 1 mg/L. All parts of seedling and callus were analyzed for their bioactive compounds and antioxidant activity using Spectrophotometer UV-Vis, whereas the other bioactive compounds were identified by Gas Chromatography-Mass Spectrophotometry. This research applied a Completely Randomized Design with sample sources of tomato sprout and callus from cotyledon and hypocotyl, with 3 replicates. The result showed that friable callus was able to be developed from both explants through the addition of NAA-Kin to MS medium. The three compounds were observed in callus and all explants. These calluses produced high antioxidant compounds from their pigments and ascorbic acid. The metabolites will be analyzed according to the perspective of their role. Major groups of compounds from GC-MS are dominated by hydrocarbons. Callus culture has a potential as the source of bioactive compounds.AbstrakSenyawa bioaktif pada tomat dapat diproduksi melalui kultur kalus, Penelitian ini bertujuan untuk mengkaji perkembangan kalus dan mengobservasi jenis senyawa bioaktif serta aktivitas antioksidan pada eksplan dan kalus. Kotiledon dan hipokotil  dari kecambah diinduksi membentuk kalus di dalam medium MS dengan penambahan NAA 2,5 mg/L dan kinetin 0,5 mg/L, maupun 2,4 D 1 mg/L tunggal.  Semua bagian kecambah dan kalus dianalisis kandungan senyawa bioaktif dan aktivitas antioksidannya dengan menggunakan Spektrofotometer UV-Vis. Sementara itu, senyawa bioaktif lainnya diidentifikasi dengan menggunakan Kromatografi Gas-Spektrofotometer Massa. Penelitian ini dilaksanakan dengan Rancangan Acak Lengkap dengan faktor sumber eksplan : kecambah, kalus yang berasal dari kotiledon maupun dari  hipokotil disertai 3 ulangan.  Hasil penelitian menunjukkan bahwa kalus remah dapat berkembang dari semua bagian kecambah pada medium MS dengan penambahan NAA dan kinetin. Terdapat tiga senyawa yang diobservasi baik pada kalus maupun sumber eksplannya. Kalus tersebut menghasilkan senyawa antioksidan yang tinggi, berdasarkan dari kandungan pigmen dan dari asam askorbat. Metabolit-metabolit tersebut akan dianalisis lebih lanjut terhadap peranannya. Kelompok senyawa yang terbanyak dari hasil GC-MS didominasi oleh hidrokarbon. Kultur kalus memiliki potensi sebagai sumber senyawa bioaktif tanaman.
Vitamin C and total soluble solid content of crystal guava at different storage duration and ripeness Khafid, Abdul; Nurchayati, Yulita; Hastuti, Endah Dwi; Setiari, Nintya
Kultivasi Vol 22, No 2 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i2.44124

Abstract

Crystal guava (Psidium guajava var. 'Crystal') fruit is in great demand because of its delicious taste and high nutritional content. Storage aims to prevent postharvest damage to the fruit. However, storage that is too long causes morphological damage and decreased nutrients. This study aims to determine the effect of storage duration, fruit ripeness stage and the interaction between both factors on vitamin C and total soluble solids (TSS) content of crystal guava, and determine which treatment can produce the highest vitamin C and TSS. Fruits harvested simultaneously with three levels of ripeness based on the skin color: unripe fruit is dark green, ripe is light green, very ripe is yellowish green. Samples selected based on the same weight range. Storage was carried out for 0, 5, and 10 days at ± 10oC. The study used a completely randomized design (CRD) with a 3x3 factorial pattern with two factors: storage duration and fruit ripeness level. Parameters observed were vitamin C, TSS, weight loss, diameter shrinkage, skin color and hardness. Data were analyzed using ANOVA and DMRT. Both treatments showed an interaction on vitamin C content. The best treatment was unripe fruit stored for ten days with 14.955 ppm of vitamin C. Both treatments did not show any interaction on TSS content. The best treatment was five days storage with TSS of 8.25 °Brix and very ripe fruit of 8.21 °Brix. Based on vitamin C, TSS content, and physical condition variables, the best guava fruit is unripe fruit stored for 10 days.Keywords: color, postharvest, physical, quality, softening
Rendemen Minyak Atsiri Rimpang Jahe Merah (Zingiber officinale var. rubrum) dengan Metode Pengeringan yang Dikombinasi dengan Kain Penutup Wiraputra, Muhammad Dwijunianto; Nurchayati, Yulita; Hastuti, Endah Dwi; Setiari, Nintya
Buletin Anatomi dan Fisiologi Volume 9, Nomor 1, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.1.2024.66-74

Abstract

Kualitas jahe merah ditentukan oleh kandungan minyak atsiri yang mudah menguap. Kombinasi cabinet solar dryer (CSD) sebagai alat pengering yang dikombinasikan dengan kain penutup dapat mempengaruhi rendemen minyak atsiri. Penelitian ini bertujuan untuk mempelajari pengaruh kombinasi metode pengeringan dengan penambahan kain penutup terhadap rendemen minyak atsiri jahe merah. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2 x 3 dengan 3 ulangan. Faktor pertama adalah pengeringan dengan cabinet solar dryer dan metode terbuka tanpa alat. Faktor kedua adalah tanpa kain, 1 lapis kain dan 2 lapis kain penutup. Parameter yang diamati adalah volume minyak atsiri, rendemen minyak atsiri, kadar air, susut bobot, suhu pengeringan, kelembaban udara, dan morfologi jahe. Metode yang digunakan adalah pengeringan rimpang jahe, yang telah diiris dengan ketebalan 5 mm. Rendemen minyak atsiri dianalisis dengan metode destilasi uap dan air. Hasil penelitian menunjukkan terdapat interaksi antara metode pengeringan dan kain penutup terhadap rendemen minyak atsiri. Rendemen tertinggi (0,98%) diperoleh dengan metode pengeringan matahari langsung dengan 1 lapis kain penutup. Kadar air terendah dan susut bobot tertinggi terdapat pada perlakuan matahari langsung tanpa kain penutup sebesar 13,04% dan 79,93%. Perlakuan optimal dalam meningkatkan volume dan rendemen minyak atsiri diperoleh pada metode pengeringan matahari terbuka dengan 1 lapis kain penutup. Metode pengeringan dengan alat CSD tanpa kain penutup menghasilkan minyak atsiri yang tinggi sebesar 0,6%. The quality of red ginger is determined by its volatile essential oil content.  A cabinet solar dryer (CSD) combined with a cloth cover can affect the yield of ginger essential oil. This study aims the effect of the drying method combined with cover cloth on the yield of red ginger essential oil. This study used a completely randomized design (CRD) factorial pattern 2 x 3 with 3 repetitions. The parameters observed were essential oil volume and yield, water content, weight loss, drying temperature, drying humidity, and ginger morphology. The method used was dried rhizome chopped with a thickness of 5 mm. The essential oils were analyzed by steam and water distillation. The results showed that there was an interaction between the two treatments for essential oil yields. The highest essential oil yield (0.98%) was obtained from direct sunlight with 1 layer of cloth. The lowest water content and highest weight loss were found in direct sun without covering cloth at 13.04% and 79.93%. Optimal treatment in increasing oil volume and essential oil yield was obtained by drying in the open sun with 1 layer of cloth cover. Drying with a CSD without a cover cloth produces a high essential oil.  
Kandungan Vitamin C dan Morfometri Buah Jambu Kristal (Psidium guajava L. cv. ‘Kristal’) pada Pengemasan yang Berbeda Putra, Agita Christyaji; Nurchayati, Yulita; Hastuti, Endah Dwi; Setiari, Nintya
Buletin Anatomi dan Fisiologi Volume 8, Nomor 2, Tahun 2023
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.8.2.2023.146-153

Abstract

Jambu kristal (Psidium guajava L. cv. ‘Kristal’) memiliki daging buah berwarna putih, tekstur renyah, tidak memiliki banyak biji serta memiliki banyak kandungan gizi, salah satunya vitamin C. Buah jambu yang sudah dipanen umumnya dikemas sebelum didistribusikan dengan tujuan untuk meminimalisir kerusakan maupun degradasi kandungan vitamin C selama penyimpanan. Penelitian ini bertujuan untuk mengetahui pengaruh jenis pengemas yang berbeda terhadap kandungan vitamin C dan morfometri buah jambu kristal. Buah dipanen pada umur 100 HSA (Hari Setelah Antesis), berdasarkan rentang ukuran dan bobot yang sama. Buah yang telah dikemas kemudian disimpan dalam lemari pendingin pada suhu ±10oC selama 3 hari. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal dengan empat perlakuan, yaitu tanpa pengemas, plastik wrapping, besek, dan styrofoam. Tiap perlakuan dengan tiga ulangan. Analisis kandungan vitamin C dilakukan dengan metode spektrofotometri. Pengamatan morfometri dengan mengukur tingkat kekerasan buah, tingkat memar buah, dan warna kulit buah. Hasil penelitian menunjukkan bahwa kandungan vitamin C tertinggi terdapat pada jenis pengemas menggunakan styrofoam sebesar 12.297 ppm dan terendah pada perlakuan plastik wrapping sebesar 8.960 ppm. Perbedaan jenis pengemas berupa plastik wrapping, besek, dan styrofoam mempengaruhi kandungan vitamin C dan morfometri buah jambu kristal. Morfometri buah seperti susut diameter, susut bobot, tingkat kekerasan, dan tingkat memar buah yang paling baik diperoleh dari jenis pengemas styrofoam.  Crystal guava (Psidium guajava L. cv. 'Kristal') has white flesh, crunchy texture, low seed content and contains high vitamin C. Harvested guava are generally packaged before distribution to minimize degradation of vitamin C content during storage. This study aims to determine the effect of different types of packages on vitamin C content and morphometry of crystal guava fruit. Fruits are harvested at 100 days after anthesis based on the same size range and weight. The packed fruit was stored in the refrigerator at ±10oC for 3 days. The study was conducted using a single factor Completely Randomized Design (CRD) with four treatments, including without packaging, plastic wrap, besek (bamboo bucket), and styrofoam. Each treatment was repeated 3 times. Analysis of vitamin C content was carried out by spectrophotometric method. The results showed that the highest vitamin C content was found in the styrofoam treatment at 12,297 ppm and the lowest in the plastic wrap treatment at 8,960 ppm. Different types of packaging in the form of plastic wrap, besek, and styrofoam affect the vitamin C content and morphometry of crystal guava fruit. The best fruit morphometry such as diameter loss, weight loss, hardness level, and bruising level was obtained from styrofoam.
RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Yulita Nurchayati
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ.
The Effect of Thidiazuron Treatment on the Growth of Vanda limbata Blume in vivo Ditasya Putri, Novika; Setiari, Nintya; Nurchayati, Yulita; Agung Suedy, Sri Widodo
Makara Journal of Science
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vanda limbata is one of the most popular ornamental orchids. Currently, its growth is slow, making it necessary to improve its cultivation techniques. One of the possible ways to achieve this is to use plant growth regulator (PGR) treatment. Thidiazuron (TDZ) is a cytokinin used to increase the growth of V. limbata. This study aimed to determine the effect of TDZ concentration on the growth rate of V. limbata. The study involved cultivating V. limbata on planting media and spraying it with TDZ at various concentrations. This research uses a CRD (Completely Randomized Design) single-factor, which is TDZ concentration at three concentrations (factor levels) of 0, 25, and 50 ppm. Data were analyzed using analysis of variance and Duncan’s Multiple Range Test at 5% significance level. The study ran for three months and the following response variables were measure: the number of new leaves, increase in length of old leaves, length of new leaves, increase in width of old leaves, width of new leaves, number of roots, and stomatal density. The results showed that TDZ significantly increased leaf length and the number of new leaves of V. limbata, but decreased the density of stomata. Thidiazuron at 50 ppm was the best treatment for increasing the growth of V. limbata.
Thidiazuron-Induced Somatic Embryogenesis in Cymbidium bicolor Orchid In Vitro Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.15306

Abstract

Cymbidium bicolor is a highly hunted and traded orchid, leading to a decline in its wild population. Orchid conservation can be achieved through tissue culture, particularly via somatic embryogenesis. Thidiazuron (TDZ) is a growth regulator used to induce somatic embryogenesis. This study aimed to determine the optimal TDZ concentration for somatic embryo formation. Stem explants of C. bicolor were cultured on Murashige Skoog (MS) medium with TDZ concentrations of 0, 1, 2, and 3 ppm. Observations were conducted weekly for two months using a stereo microscope and OptiLab. Variables observed included the percentage of green explants, somatic embryo formation time, the number of explants forming somatic embryos, and the number and morphology of somatic embryos. The study was arranged in a Completely Randomized Design (CRD) with 14 replications. Results showed that TDZ addition influenced somatic embryo formation and maintained the green color of explants. Media with TDZ promoted faster growth and larger embryo size compared to media without TDZ. The optimal concentration was 1 ppm TDZ, which produced the highest number of embryos (172) and the fastest formation time compared to other concentrations (TDZ 0: 27, TDZ 2 ppm: 60, TDZ 3 ppm: 39).
Somatic Embryo Enhancing of Phalaenopsis amabilis (L.) Blume Orchid with 6-Benzyl Amino Purine (BAP) Karend, Azzah Hanun Abas; Setiari, Nintya; Nurchayati, Yulita; Rashid, Fatimah Azzahra Ahmad
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p88-98

Abstract

Phalaenopsis amabilis (L.) Blume is an orchid with high economic value because of its beautiful white flowers, yellow labellum, and long stems. Its existence in nature is threatened with extinction, so efforts are needed to cultivate and preserve P. amabilis, one of which is through somatic embryos, which generally require Plant Growth Regulators (PGRs) in the form of 6-Benzyl Amino Purine (BAP). 6-Benzyl Amino Purine (BAP) is used because it is one of the cytokinin hormones proven to induce somatic embryos. This study aims to examine and determine the use of the right BAP concentration to propagate P. amabilis through somatic embryo induction. The explants used were P. amabilis protocorms; because protocorms produced a greater number of somatic embryos than leaf explants, the development of the explants was observed every week for 2 months. This study used a single factor Completely Randomized Design (CRD) in the form of BAP concentrations of 0, 1, 2, 3, and 4 ppm with 20 replications. The results showed that the most optimal treatment for the propagation of P. amabilis somatic embryos was on media with an average number of somatic embryos at a concentration of BAP 3 ppm (65.00) embryo/explain.
Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Indriani, Reni; Prihastanti, Erma; Budihastuti, Rini; Nurchayati, Yulita
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
Effect of Different Coconut Water Concentrations on the Growth of Dendrobium spectabile (Blume) Miq. Planlet on MS Media Santosa, Faradita Azzahra; Setiari, Nintya; Nurchayati, Yulita
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 3 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.3.533

Abstract

Dendrobium spectabile is an orchid with unique flowers that are vulnerable to exploitation. Tissue culture is employed to conserve this orchid, where the type of medium and organic supplements significantly affect plantlet growth. Murashige and Skoog (MS) media was added with coconut water as an exogenous hormone source. This study aimed to determine the effect of adding coconut water and determine the optimal concentration to increase the growth of D. spectabile at the subculture stage. The method involved subculturing D. spectabile plantlets in vitro in MS media supplemented with coconut water at concentrations of 0%, 5%, 10%, 15%, and 20%. The research design used a completely randomized design (CRD), with coconut water concentration and ten replicates. Data were analyzed using ANOVA and Duncan's Multiple Range Test at a 5% significance level. Plantlet growth was observed for two months, and the parameters observed were the emergence of new shoots, leaves, and roots; the number of new shoots, leaves, and roots; and the length of new leaves. The results showed that the addition of coconut water to the subculture medium can increase the leaf growth of D. spectabile orchid plantlets but does not increase shoot and root growth. Coconut water 10% was the most optimal concentration for the number of new leaves and new leaf length. The addition of 15% coconut water was the most optimal concentration for plantlet new leaf emergence. Coconut water plays a vital role in in vitro culture, supporting the various growth processes of D. spectabile plantlets. Keywords: Dendrobium spectabile, ex situ conservation, organic supplement, subculture
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Azura Muzdalifah Istiqomah Diah Nurmaningrum Ditasya Putri, Novika Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Indriani, Reni Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Mochammad Syaifuddin Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Nur Cahyani, Evi Prameswari, Ratna Apriliani Puji Nur Hana Putra, Agita Christyaji Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Safinah, Safinah Santosa Santosa Santosa Santosa Santosa, Faradita Azzahra Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto