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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH Biosfer: Jurnal Tadris Biologi Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Planta Tropika Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati IJEMS (Indonesian Journal of Environmental Management and Sustainability) Biosaintifika: Journal of Biology & Biology Education Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Yulita Nurchayati
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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The Effect of Thidiazuron Treatment on the Growth of Vanda limbata Blume in vivo Ditasya Putri, Novika; Setiari, Nintya; Nurchayati, Yulita; Agung Suedy, Sri Widodo
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vanda limbata is one of the most popular ornamental orchids. Currently, its growth is slow, making it necessary to improve its cultivation techniques. One of the possible ways to achieve this is to use plant growth regulator (PGR) treatment. Thidiazuron (TDZ) is a cytokinin used to increase the growth of V. limbata. This study aimed to determine the effect of TDZ concentration on the growth rate of V. limbata. The study involved cultivating V. limbata on planting media and spraying it with TDZ at various concentrations. This research uses a CRD (Completely Randomized Design) single-factor, which is TDZ concentration at three concentrations (factor levels) of 0, 25, and 50 ppm. Data were analyzed using analysis of variance and Duncan’s Multiple Range Test at 5% significance level. The study ran for three months and the following response variables were measure: the number of new leaves, increase in length of old leaves, length of new leaves, increase in width of old leaves, width of new leaves, number of roots, and stomatal density. The results showed that TDZ significantly increased leaf length and the number of new leaves of V. limbata, but decreased the density of stomata. Thidiazuron at 50 ppm was the best treatment for increasing the growth of V. limbata.
Thidiazuron-Induced Somatic Embryogenesis in Cymbidium bicolor Orchid In Vitro Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.15306

Abstract

Cymbidium bicolor is a highly hunted and traded orchid, leading to a decline in its wild population. Orchid conservation can be achieved through tissue culture, particularly via somatic embryogenesis. Thidiazuron (TDZ) is a growth regulator used to induce somatic embryogenesis. This study aimed to determine the optimal TDZ concentration for somatic embryo formation. Stem explants of C. bicolor were cultured on Murashige Skoog (MS) medium with TDZ concentrations of 0, 1, 2, and 3 ppm. Observations were conducted weekly for two months using a stereo microscope and OptiLab. Variables observed included the percentage of green explants, somatic embryo formation time, the number of explants forming somatic embryos, and the number and morphology of somatic embryos. The study was arranged in a Completely Randomized Design (CRD) with 14 replications. Results showed that TDZ addition influenced somatic embryo formation and maintained the green color of explants. Media with TDZ promoted faster growth and larger embryo size compared to media without TDZ. The optimal concentration was 1 ppm TDZ, which produced the highest number of embryos (172) and the fastest formation time compared to other concentrations (TDZ 0: 27, TDZ 2 ppm: 60, TDZ 3 ppm: 39).
Somatic Embryo Enhancing of Phalaenopsis amabilis (L.) Blume Orchid with 6-Benzyl Amino Purine (BAP) Karend, Azzah Hanun Abas; Setiari, Nintya; Nurchayati, Yulita; Rashid, Fatimah Azzahra Ahmad
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p88-98

Abstract

Phalaenopsis amabilis (L.) Blume is an orchid with high economic value because of its beautiful white flowers, yellow labellum, and long stems. Its existence in nature is threatened with extinction, so efforts are needed to cultivate and preserve P. amabilis, one of which is through somatic embryos, which generally require Plant Growth Regulators (PGRs) in the form of 6-Benzyl Amino Purine (BAP). 6-Benzyl Amino Purine (BAP) is used because it is one of the cytokinin hormones proven to induce somatic embryos. This study aims to examine and determine the use of the right BAP concentration to propagate P. amabilis through somatic embryo induction. The explants used were P. amabilis protocorms; because protocorms produced a greater number of somatic embryos than leaf explants, the development of the explants was observed every week for 2 months. This study used a single factor Completely Randomized Design (CRD) in the form of BAP concentrations of 0, 1, 2, 3, and 4 ppm with 20 replications. The results showed that the most optimal treatment for the propagation of P. amabilis somatic embryos was on media with an average number of somatic embryos at a concentration of BAP 3 ppm (65.00) embryo/explain.
Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Indriani, Reni; Prihastanti, Erma; Budihastuti, Rini; Nurchayati, Yulita
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
Effect of Different Coconut Water Concentrations on the Growth of Dendrobium spectabile (Blume) Miq. Planlet on MS Media Santosa, Faradita Azzahra; Setiari, Nintya; Nurchayati, Yulita
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 3 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.3.533

Abstract

Dendrobium spectabile is an orchid with unique flowers that are vulnerable to exploitation. Tissue culture is employed to conserve this orchid, where the type of medium and organic supplements significantly affect plantlet growth. Murashige and Skoog (MS) media was added with coconut water as an exogenous hormone source. This study aimed to determine the effect of adding coconut water and determine the optimal concentration to increase the growth of D. spectabile at the subculture stage. The method involved subculturing D. spectabile plantlets in vitro in MS media supplemented with coconut water at concentrations of 0%, 5%, 10%, 15%, and 20%. The research design used a completely randomized design (CRD), with coconut water concentration and ten replicates. Data were analyzed using ANOVA and Duncan's Multiple Range Test at a 5% significance level. Plantlet growth was observed for two months, and the parameters observed were the emergence of new shoots, leaves, and roots; the number of new shoots, leaves, and roots; and the length of new leaves. The results showed that the addition of coconut water to the subculture medium can increase the leaf growth of D. spectabile orchid plantlets but does not increase shoot and root growth. Coconut water 10% was the most optimal concentration for the number of new leaves and new leaf length. The addition of 15% coconut water was the most optimal concentration for plantlet new leaf emergence. Coconut water plays a vital role in in vitro culture, supporting the various growth processes of D. spectabile plantlets. Keywords: Dendrobium spectabile, ex situ conservation, organic supplement, subculture
The Impact of Biochar and FeCl3 Administration on Fe Toxicity Symptoms and Productivity of Bok Choy Mustard Plants (Brassica rapa L.) Nauli Variety Prameswari, Ratna Apriliani; Nurchayati, Yulita; Setiari, Nintya
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 3 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.3.447

Abstract

Fe³⁺ pollution is a factor that reduces bok choy production. Its productivity can be increased by adding biochar, which can adsorb inorganic ions, including iron. This study examines the impact of rice husk biochar and FeCl₃ on bok choy productivity, using a completely randomized design with a 3´3 factorial pattern. The first factor was the concentration of rice husk biochar (0, 2.5, and 7.5 g/kg), and the second was the concentration of FeCl₃ (0, 25, and 50 mg/L). The criteria measured were the number of leaves, leaf area, chlorophyll content, and vitamin C levels. The combination of 0 g/kg biochar and 0 mg/L FeCl₃ proved to be the most effective treatment for boosting bok choy growth. The treatment yielded 12.6 leaf count, 73.3 cm² leaf area, 2.0 mg/g chlorophyll, and 30.8 ppm vitamin C. Fe³⁺ treatments at 25–50 mg/L drastically lowered all growth metrics. The applied biochar doses were insufficient to efficiently absorb Fe³⁺ and boost the yield of bok choy. Keywords: biochar, bok choy, chinese mustard, FeCl3, toxicity
Peningkatan Pertumbuhan Anggrek Phalaenopsis amabilis (L.) Blume secara In Vivo setelah Pemberian Thidiazuron (TDZ) Nur Cahyani, Evi; Nintya Setiari; Yulita Nurchayati
Jurnal Hortikultura Indonesia (JHI) Vol. 16 No. 2 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.2.92-99

Abstract

Phalaenopsis amabilis (L.) Blume merupakan salah satu jenis anggrek yang banyak dimanfaatkan sebagai tanaman hias dan sebagai spesies induk dalam program pemuliaan. Eksploitasi yang berlebihan serta laju pertumbuhan vegetatif yang lambat menjadikan anggrek ini memerlukan upaya konservasi ex-situ melalui penambahan nutrisi dan zat pengatur tumbuh, salah satunya Thidiazuron (TDZ). Penelitian ini bertujuan untuk mengkaji pengaruh serta menentukan konsentrasi TDZ yang optimal terhadap pertumbuhan P. amabilis. Penelitian dilaksanakan pada November 2023 hingga Januari 2024 di Tembalang, Semarang, menggunakan Rancangan Acak Lengkap (RAL) satu faktor, yaitu konsentrasi TDZ (0, 25, dan 50 ppm). Parameter yang diamati meliputi panjang dan lebar daun lama, jumlah dan waktu munculnya daun baru, warna daun, jumlah dan waktu munculnya akar baru, serta densitas stomata. Data dianalisis menggunakan ANOVA dan dilanjutkan dengan uji Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa pemberian TDZ berpengaruh nyata terhadap peningkatan panjang dan lebar daun lama, jumlah dan waktu munculnya daun baru, serta densitas stomata P. amabilis. Thidiazuron (TDZ) juga memengaruhi perubahan warna daun. Konsentrasi TDZ 25 ppm merupakan perlakuan terbaik dalam meningkatkan pertumbuhan daun P. amabilis. Kata kunci: konservasi, sitokinin, vegetatif, zat pengatur tumbuh
The Effect of Benzyl Amino Purine (BAP) On Potato (Solanum tuberosum L.) Axillary Buds Micropropagation Putri Anugrah Maulidya; Nurchayati, Yulita; Setiari, Nintya
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 01 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss01/480

Abstract

Potato (Solanum tuberosum L.) production in Indonesia increased by 4.21% in 2022 compared to the previous year, but this production is still low by international standards. This is caused by low quality and quantity of potato seeds which is sourced from tubers. It is necessary to modify the in vitro cultivation of potato seed sources by utilising seeds that are often wasted with the addition of cytokinins, such as Benzyl Amino Purine (BAP). The aim of this study was to determine the effect of BAP on potato axillary shoot explants sourced from seeds. The study was conducted using a Completely Randomized Design with one factor, namely BAP concentrations of 0, 1, 2 and 3 ppm with 3 replications. The results showed that there is an influence of BAP on the parameters of shoot growth percentage, leaf and root emergence time, plant height, number of shoots, roots, and leaves of potato axillary buds planlet. The 2 ppm BAP treatment tends to give the best response in increasing the number of shoots with an average of 4.33 shoots, making it effective for producing potato seedlings from axillary buds in vitro.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
Pertumbuhan Anggrek Cymbidium ensifolium (L.) Sw. dengan Pemberian Pupuk Hayati Mikoriza Misrofah, Sofatun; Misrofah, Sofatu; Setiari, Nintya; Nurchayati, Yulita; Suedy, Sri Widodo Agung
Jurnal Hortikultura Indonesia (JHI) Vol. 13 No. 1 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.1.35-42

Abstract

ABSTRAKAnggrek Cymbidium ensifolium adalah anggrek terestrial yang terdaftar di CITES 2020 sebagai spesies hampir terancam punah atau rentan sehingga perlu upaya pelestarian secara ex situ. Salah satu faktor pendukung budidaya anggrek adalah pemberian pupuk. Pupuk hayati mikoriza bersifat ramah lingkungan dan dapat mengurangi dampak negatif pupuk anorganik. Penelitian ini bertujuan untuk mengkaji pengaruh pupuk hayati mikoriza berbagai dosis terhadap pertumbuhan anggrek C. ensifolium. Penelitian ini disusun menggunakan Rancangan Acak Lengkap satu faktor, yaitu pupuk hayati mikoriza (0; 5; 10; 15 g tanaman-1) dengan 6 ulangan. Data dianalisis pada Analysis of Variance dengan taraf kepercayaan 95%. Percobaan dilakukan bulan Oktober 2021 – Februari 2022 di Tembalang, Semarang. Parameter yang diamati berupa waktu muncul tunas, jumlah tunas, waktu muncul daun, jumlah daun, dan persentase infeksi mikoriza. Hasil penelitian menunjukkan bahwa perlakuan pupuk hayati mikoriza berpengaruh signifikan terhadap waktu muncul tunas dan daun, jumlah daun, dan persentase infeksi mikoriza. Pupuk hayati mikoriza sebesar 5 g tanaman-1 merupakan perlakuan optimal untuk meningkatkan waktu muncul tunas dan daun, serta konsentrasi 15 g tanaman-1 optimal meningkatkan jumlah daun dan persentase infeksi mikoriza. Pengaruh pupuk hayati mikoriza ini dapat dikembangkan untuk pelestarian anggrek jenis lain. Kata kunci: anggrek spesies, Cymbidium, biofertilizer, mikoriza
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Diah Nurmaningrum Ditasya Putri, Novika Einstivina Nuryandani Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur Hartati, Puji I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Indriani, Reni Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Misrofah, Sofatu Misrofah, Sofatun Mochammad Syaifuddin Muhammad Syaiful Anam Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Noveira, Ameviana Fitri Nur Cahyani, Evi Prameswari, Ratna Apriliani Puji Nur Hana Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Santosa Santosa Santosa Santosa Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utama, Rafii Satria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Zahrotunnisa, Alfi Zakiyah, Aisyah