Article Per Year (5 Year)
p-Index From 2020 - 2025
3.366
P-Index
This Author published in this journals
All Journal Jurnal Biologi Udayana AL KAUNIYAH Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) BIOMA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) BIOPROSPEK Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Ilmiah Universitas Batanghari Jambi Biosaintifika: Journal of Biology & Biology Education Biosel: Biologi Science and Education JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Jurnal Riset Biologi dan Aplikasinya Infokes : Jurnal Ilmiah Rekam Medis dan Informasi Kesehatan Bioscientist : Jurnal Ilmiah Biologi JURNAL STUDIA ADMINISTRASI KESANS : International Journal of Health and Science Informatics, Electrical and Electronics Engineering Journal of Social Outreach Jurnal Natural JATIMAS : Jurnal Pertanian dan Pengabdian Masyarakat
Imam Rosadi
Department Of Biology, Faculty Mathematics And Natural Sciences, Mulawarman University
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

Published : 30 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Biosel: Biologi Science and Education

 1 
The Substitution Effect of Fetal Bovine Serum to Platelet-Rich Plasma on Stem Cell Proliferation Rosadi, Imam; Karina; Afini, Irsyah; Widyastuti, Tias; Sobariah, Siti
BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan Vol. 13 No. 2 (2024): BIOSEL (Biology Science and Education: Jurnal Penelitian Science dan Pendidika
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v13i2.7400

Abstract

Stem cells are capable of self-regeneration and differentiation into specialized cells, such as adipocytes, chondrocytes, and osteocytes. Stem cells can be cultured in vitro using media-containing serum, such as fetal bovine serum (FBS). However, the cell yield is often lacking, thus the manipulation of the culture medium is needed. Platelet-rich plasma (PRP) contains a thousand growth proteins. This study aims to compare the effects of FBS (Gibco), non-commercial PRP, and commercial PLTGold® Human Platelet Lysate (Merck) on the growth and population doubling time (PDT) of stem cells isolated from adipose tissue. Stem cells will be enzymatically isolated from adipose tissue, followed by culture and expansion processes, with subsequent observations of cell morphology, viability, growth rate, and PDT. The results showed that adipose-derived stem cells exhibit fibroblast-like morphology and adhere to the plastic surface. The optimal cell number, PDT and proliferation rate showed in PLTGold®, PRP and FBS respectively. Based on these results, it can be concluded that PLTGold® or PRP can substitute FBS as a growth supplement for stem cell proliferation.  Keywords: Stem Cell, Proliferation, PDT, PRP, PLTGold®
Co-Authors Abriyanto, Hery Adlia Khalisha Afini, Irsyah Al Hasbi, Ezra Alam, Megawati Citra Alfida Zakiyah An Nissa Falaq Qurrahmah An Nissa falaq Qurrahmah Anggraini Barlian Anggraini Barlian Arief Lelono Arum Aryani, Retno Cici Lis Qurrotun Ayuni Crisnia Crisnia Cyndi Marcelina DEMO, HILDIANA APRILIANI DHIU Dewi Elfidasari Dwi Atmi Narwati Edy Kustiani Fawaidul Badri Habib Pangeran Hamisya Nurindriani Haroh, Istik Hazmi, Nubli Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Indah Nurcahyani Iqbal Purnama, Muhammad Irene Putri Pricillia Butar-Butar Irene Putri Priscilla Butar-Butar Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Karina Karina Karina Karina Karina Karina Karina Komang A Wahyuningsih Komang A Wahyuningsih Komang A. Wahyuningsih Komang A. Wahyuningsih Komang A. Wahyuningsih Linda Oktavianingsih Linda Oktavianingsih Masayu Nadhira Muhammad Fauzi Arif Muhammad Fauzi Arif Muhammadiyah Muhammadiyah Mukh Syaifudin Ngatmari Nita Noriko Nova Hariani Nur Ulmi Olivia Yolanda Lawono Panggulu Ahmad Ramadhani Utoro Pratiwi, Anggita Endar Putranto, Bima Arya Putri Inayah Aisyah Rahayu, Sekar Riris Lindiawati Puspitasari Rizka Shofiyya Ramadhani Rosliana, Iis Rudy Agung Nugroho Satriya Bayu Aji, Satriya Bayu Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Sobariah, Siti Sugianur, Sugianur Supandji, Supandji Tias Widyastuti Tias Widyastuti Tias Widyastuti Tias Widyastuti Tias Widyastuti Umi Mutfidasari Umilasari, Reni Wafif Azizah Wahyuningsih, Komang A Widi Artini Widyastuti, Tias Wismo Reja Subroto Yuliani, Ferdina Yuliatin, Ervinda