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All Journal Jurnal Gizi dan Pangan JURNAL GIZI INDONESIA JURNAL SISTEM INFORMASI BISNIS Journal of Nutrition College Journal of Biomedicine and Translational Research agriTECH Jurnal Media Gizi Indonesia (MGI) Nutri-Sains: Jurnal Gizi, Pangan dan Aplikasinya Medical Laboratory Technology Journal AcTion: Aceh Nutrition Journal Journal of Applied Food Technology Journal of Health and Nutrition Research Poltekita: Jurnal Ilmu Kesehatan Jurnal Info Kesehatan
Ninik Rustanti
Program Studi Ilmu Gizi, Fakultas Kedokteran, Universitas Diponegoro, Semarang 50275
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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Journal : Medical Laboratory Technology Journal

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Antibacterial Potential of Curcuma Mangga Kombucha: The Effect of Fermentation Duration on Activity Against Escherichia coli and Salmonella typhi Ratna, Ratna; Anjani, Gemala; Nur Afifah, Diana; Ayustaningwarno, Fitriyono; Rustanti, Ninik
Medical Laboratory Technology Journal Vol. 11 No. 1 (2025): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i1.636

Abstract

Kombucha is a fermented beverage known for its various health benefits, including antibacterial activity. This study aims to evaluate the antibacterial potential of mango ginger (Curcuma mangga)-based kombucha against Escherichia coli and Salmonella typhi, as well as to analyze the effect of fermentation duration on the physicochemical properties of kombucha. Fermentation was conducted for 5, 7, 10, and 14 days, with total acidity, pH, and antibacterial activity measurements using the well diffusion method. The results showed total acidity increased as fermentation progressed, while pH decreased. Antibacterial tests demonstrated that mango ginger kombucha exhibited inhibitory activity against Escherichia coli and Salmonella typhi, with the largest inhibition zones observed on day 10 of fermentation (8.2 mm for Escherichia coli and 9.2 mm for Salmonella typhi). Although its inhibitory effect was moderate compared to the positive control (ciprofloxacin), these findings suggest the potential of mango ginger kombucha as a functional beverage with antibacterial properties. Further research is needed to identify the active compounds responsible for its antimicrobial activity and to optimize the fermentation process to enhance its effectiveness.
The Effects of Fermented Buffalo Milk (Dadih) Fortified with Red Dragon Fruit and Selenium on Adiponectin and Tumor Necrosis Factor-Alpha Levels in Obese Rats Wulandari, Fitri; Rustanti, Ninik; Pramono, Adriyan
Medical Laboratory Technology Journal Vol. 11 No. 1 (2025): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i1.639

Abstract

The increasing prevalence of obesity and its associated complications necessitates the exploration of functional foods as therapeutic interventions. This study aimed to analyze the effects of fermented buffalo milk (dadih) fortified with red dragon fruit (Hylocereus polyrhizus) and selenium on adiponectin and Tumor Necrosis Factor-Alpha (TNF-α) levels in obese rats. This experimental study employed a pretest-posttest control group design involving 20 Wistar rats divided into four groups: K1 (healthy control), K2 (obese control), P1, and P2 (obese intervention). K2, P1, and P2 were in obesity conditions induced by a high-fat-fructose diet (HFFD) for 28 days, and K1 was a healthy control group. Furthermore, dadih only and dadih fortified with red dragon fruit and selenium at 1.8 g/200 g body weight/day were administered to P1 and P2 groups, respectively. Treatment using dadih fortified with red dragon fruit and selenium significantly reduced weight gain (p=0.000) compared to K2 and P1. In addition, the P2 group showed increased adiponectin levels and decreased TNF-α levels (p=0.000) compared to the K2 and P1 groups. A strong negative correlation was found between adiponectin levels and TNF-alpha and body weight, while TNF-alpha and body weight showed a strong positive correlation. To conclude, fortifying dadih with red dragon fruit and selenium is more effective in improving the metabolic condition of obese rats compared to non-fortified dadih. This fortification significantly enhances adiponectin levels and reduces TNF-alpha levels. These findings suggest that functional food fortification could be a promising approach for managing obesity-related inflammation and metabolic disorders in humans, highlighting the potential application of dadih in dietary interventions.
Co-Authors Adriyan Pramono Agung Dwi Prasetyo Agung Dwi Prasetyo, Agung Dwi Andriana Murdi Hastuti Ani Margawati Anjani, Gemala Annisa Istiqomah, Annisa Armelinda, Nyoman Putri Ari Aryu Candra Aulia, Salma Shafrina Avisha, Rosita Nur Ayu Rahadiyanti Ayu, Azzahra Mutiara Ayudya Luthfia Nintami Christanto, Monica Lemuela Deny Yudi Fitranti Dewi Marfu’ah Kurniawati Dewi, Sonia Kurnia Dhiyas, Ardhanareswari Dian Estiningtyas Dian Isti Cahyani, Dian Isti Diana Nur Afifah, Diana Nur Elvizahro Leiyla Endang Sri Lestari Endang Sri Lestari Etika Ratna Noer Fadhila, Farah Rosyihana Fala, Etika Nurul Febriandina, Kharisma Dwi Firdha Ayu Trianie Fitri Wulandari Fitriyono Ayustaningwarno Hajar Surya Wardani Hardaning Ausiea Marantha Hartanti Sandi Wijayanti Hestin Rahmawati Ina Susanti Indah Mega Astuti Indriyanti, Risa Latifah Nur Aisiyah Magfirah, Ariel Maria Annisa Primawestri Marsella Temala Dewi MARTHA ARDIARIA Mukarromah, Tsamaroh Azzah Mursid Tri Susilo Muthia Fauzan Nafsih, Vifin Zakiahtin Nissa, Choirun Nur Afifah, Diana Nur Ahmad Habibi Nur Ilaika Zulfa Nurul Alam Sayekti Nuryanto Nuryanto . Nuryanto Nuryanto Oky Dwi Nurhayati Puspadani, Nurlina Rachma Purwanti Ramayani, Gita Ratih Paramastuti Ratna Ratna Rayyani Zaena Dini Resmi Unggul Pratiwi, Resmi Unggul Rozanah, Ishmah S. Sugiharto Safitri, Ulan Sidhin, Syaharani Sinaga, Millennia Dorais Sinthika, Euodia Sugiharto Suryaningrum, Trisna Ulil Albab Utami Harjantini, Utami Velicia, Velicia Yustitie, Normasari