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All Journal Journal of Marine and Aquatic Sciences Jurnal Ilmu Lingkungan AL KAUNIYAH El-Hayah : Jurnal Biologi Biogenesis: Jurnal Ilmiah Biologi Jurnal Sumberdaya Hayati BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Sains Jurnal Biodjati BIOTROPIC The Journal of Tropical Biology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosel: Biologi Science and Education Jurnal Riset Akuakultur Scripta Biologica Pharmaceutical Journal of Indonesia JATI (Jurnal Mahasiswa Teknik Informatika) Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang Jurnal Sains Teknologi dalam Pemberdayaan Masyarakat Academic Journal of Computer Science Research (AJCSR) SAINSTECH: Jurnal Penelitian dan Pengkajian Sains dan Teknologi Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Firdaus Ramadhan
Laboratorium Mikrobiologi, Fakultas Farmasi, Institut Sains Dan Teknologi Nasional (ISTN)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Other

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Journal : Jurnal Bioteknologi

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ANTIBAKTERI EKSTRAK KAPANG ENDOFIT DARI AKAR KAYU JAWA (Lannea coromandelica (Houtt.) Merr.) Saiful Bahri; Puteri Amelia; Normala Rachmawati; Aulia Fitri Firdausya; Firdaus Ramadhan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.107 KB) | DOI: 10.29122/jbbi.v8i2.4855

Abstract

Isolation of endophytic fungi from the roots of Lannea coromandelica could be one way to know the presence of antibacterial activity that serves as a traditional medicinal remedy. This study aims to determine the presence of endophytic fungi at the root of L. coromandelica and their extract potential as an antibacterial agent. Endophytic fungi isolation was performed by direct technique and their macroscopic as well as microscopic characteristics were observed. Screening tests were conducted using methanol and ethyl acetate solvents. Antibacterial tests were conducted on Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis. This study resulted in seven isolates of endophytic fungi (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) originated from the root of L. coromandelica. RLC 5 isolate exhibited the largest diameter of inhibition activity on the screening tests and the largest diameter on the antibacterial tests. The supernatant extract of RLC 5 isolate showed the highest antibacterial activity against the test bacterium S. epidermidis. Isolasi kapang endofit dari akar Lannea coromandelica dapat menjadi salah satu cara untuk mengetahui adanya aktivitas antibakteri yang berfungsi sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui keberadaan kapang endofit pada akar L. coromandelica dan potensinya sebagai agen antibakteri. Isolasi kapang endofit dilakukan dengan teknik langsung lalu diamati karakteristik makroskopik dan mikroskopiknya. Uji penapisan dilakukan menggunakan pelarut metanol dan etil asetat. Uji antibakteri dilakukan terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus epidermidis. Penelitian ini mendapatkan tujuh isolat kapang endofit (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) yang berasal dari akar L. coromandelica. Isolat RLC 5 memperlihatkan diameter daya hambat terbesar pada uji penapisan dan diameter terbesar pada uji aktivitas antibakteri. Ekstrak supernatan isolat RLC 5 menunjukkan aktivitas antibakteri tertinggi terhadap bakteri uji S. epidermidis.
Co-Authors Ahmad Zulfikar Wicaksono Ahmad Zulfikar Wicaksono Aina Nadila Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin Alfan Farhan Rijaluddin, Alfan Farhan Alfianur Azmi Muhammad Amelia, Puteri Ardian Khairiah Ario Putro Pamungkas Arrum Sekar Diningrum Assuyuti, Yayan Mardiansyah Attamimi, Febi Irfaullah Aulia Fitri Firdausya Bahtiar, Nur Pajar Wahyu Dinda Rama Haribowo Dinda Rama Haribowo Dwi Cahya Kusuma Dwikencana, Windy Eka Putri, Lily Surayya Febrinita, Filda Fikriwansyah, Aswir Ghoza, Muhammad Radian Hajar Indra Wardhana Hariwibowo, Dinda Rama Haulani, Muttharif Arrabie Hutauruk, Hendra Johanes I Ketut Suada Indhina Reihannisa Indhina Reihannisa, Indhina Irawan Sugoro Irianti, Ewith Ratih Isral, Isral Jarudin Jarudin Kartika Lestari, Anggita Rana Sasmita Lily Surayya Eka Putri, Lily Surayya Eka Lukman, Jihan Fadhilah Mardi ansyah Mardiansyah Mardiansyah, Mardiansyah Marpaung, Albert Anggiat Petrus Mudrika, Dadan Muhammad Ikhsan Muhammad Ridhwan Muhammad, Alfianur Azmi Mujiyanto, Aldi Nani Radiastuti Naufal, Muhamad Nia Dzirwatul Izza Normala Rachmawati Nugraha, Slamet Nur Kholidah Nurkholidah Nurkholidah, Nurkholidah Pandu Kusuma, Abdi Pratiwi Amalia Zahrah Priyanti Priyanti Rachma Fauziah Rahayu Kusuma Ningrum Ramadhani, Lidia Anggita Ratih Mayangsari Reza Bayu Zikrillah Reza Bayu Zikrillah Riliansyah, Armar Rizky Aprizal Rosario T Manalu Ryandra, Raka Saiful Bahri Saiful Bahri Saiful Bahri Saiful Bahri Sari, Arina Findo Shalsabilla, Syalwa Ersadiwi Sholeha, Dania Refia Riska Siregar, Barharuddin Solihat, Nur Amaliah Sukarman Sukarman Susan Maphilindawati Noor Sutarman Sutarman Syifa Annisa Taqiyuddin Zanki Haidar Tokeshi, Mutsunori Yayan Mardiansyah Assuyuti