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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Ilmu Ternak JURNAL GALUNG TROPIKA Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pertanian Agros Biosel: Biologi Science and Education Jurnal Hutan Pulau-Pulau Kecil : Jurnal Ilmu-Ilmu Kehutanan dan Pertanian Agrinimal Jurnal Ilmu Ternak dan Tanaman Jurnal Agrosilvopasture-Tech Rumphius Pattimura Biological Journal Maanu : Jurnal Pengabdian Kepada Masyarakat
Insun Sangadji
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics Veterinary

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Journal : Jurnal Veteriner

 1 
Fetal Bovine Serum dalam Pengencer Tris Mempertahankan Kehidupan dan Keutuhan Membran Plasma Spermatozoa Semen Beku Domba Garut (FETAL BOVINE SERUM IN TRIS EXTENDER MAINTAINING SPERMATOZOA VIABILITY AND PLASMA MEMBRANE INTEGRITY OF GARUT RAM FROZEN SEMEN Muhammad Rizal; Herdis .; Insun Sangadji
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to examine the effectiveness of fetal bovine serum (FBS) against thequality of garut ram frozen semen. Semen was collected from one mature garut ram using artificial vagina.Fresh semen were evaluated then divided into four tubes at equal volume and each tube were diluted withTris extender containing 20% egg yolk (TEY-20, as control); TEY-20 + 8% FBS (FBS-8); TEY-20 + 10% FBS(FBS-10); and TEY-20 + 12% FBS (FBS-12), respectively. Semen at the concentration of 100x106 motilespermatozoa was loaded in 0.25 ml mini straw. Semen was equilibrated at 50C for three hours, then freezeand stored in liquid nitrogen container. The quality of the spermatozoa including percentages of motileand live spermatozoa, intact plasma membrane (IPM) were evaluated following diluting, equilibratingand thawing process. A Complete Randomized design using four treatments and five replicates were usedin the study. The results showed that there was no significant difference (p<0.05) in percentage of motilespermatozoa following thawing between the control (44.0%) and FBS-8 (46.0%), FBS-10 (48.0%), andFBS-12 (47.0%), respectively. The percentage of live spermatozoa and IPM were significantly higher (p<0.05)in the FBS-8 (69.0% and 58.2%); FBS-10 (72.4% and 61.2%); FBS-12 (72.2% and 64.4%) compared to thecontrol (64.8% and 52.8%), respectively. In conclusion, the addition of FBS into Tris extender was able tomaintain the viability and integrity of plasma membrane of garut ram frozen semen.
Kriopreservasi Semen Domba Garut dengan Pengencer Tris yang Disuplementasi Ethylene Diamine Tetraacetic Acid (CRYOPRESERVATION OF GARUT RAM SEMEN WITH TRIS EXTENDER ETHYLENEDIAMINETETRAACETIC ACID) Muhammad Rizal; Herdis .; Nasrullah .; Muhammad Riyadhi; Insun Sangadji; Yulnawati .
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Ethylene diamine tetraacetic acid (EDTA) is a molecular structure that binds to heavy metals andother toxins which needed to protect the spermatozoa during the cryopreservation process. The objectiveof this research was to examine EDTA in maintain the quality of garut ram frozen semen. Semen werecollected from mature Garut ram by artificial vagina. Fresh semen were evaluated and divided in equalvolume into three tubes and diluted with Tris extender containing 20% egg yolk (TEY-20) as control, TEY-20 + 0.01% EDTA (EDTA0.01), and TEY-20 + 0.02% EDTA (EDTA0.02), respectively. Semen was loaded in 0.25 mL mini straw with the concentration of 100 million motile spermatozoa. Semen was equilibrated at5oC for three hours, then frozen and stored in liquid nitrogen container. Quality of spermatozoa includingpercentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) wereevaluated after diluting, equilibrating, and thawing. Data were analyzed using completely randomizeddesign with three treatments and five replicates. Means were compared significant difference test at 0.05significant level. Results of this research showed that mean volume, color, consistency, pH, mass movement,spermatozoa concentration, percentage of MS, percentage of LS, percentage of abnormal spermatozoa,and percentage of IPM of garut ram fresh semen were 0.87 mL, cream, thick, 6.98, 3, 4,296 million cell/mL,75%, 86.33%, 4.4%, and 85%, respectively. Percentages of MS, LS, and IPM after thawing in control (42.5,52.5, and 52.17%) was not significantly difference with EDTA0.01 (44.17, 52.5, and 52.5%) and EDTA0.02(39.17%, 51%, and 48.33%). In conclusion, the addition of EDTA in Tris extender can maintain quality ofGarut ram frozen semen, and its suitable using in artificial insemination program. Addition of EDTA cannot enhancing quality of garut ram frozen semen.
Co-Authors Aminuddin Parakkasi Arpipi, Henderika S. S. Gaspersz, Nelson Hattu, Nikmans Herdis . Joris, Lily Kapelle, Imanuel B. D. Kayadoe, Ferens Kilmanun, Nicodemus Gidion Komang G. Wiryawan Matatula, Michel Muhammad Rijal Muhammad Rivai Muhammad Riyadhi Muhammad Rizal Nasrullah . Patty, Christian W Patty, Christian W. Patty, Christian Willem Ralahalu, Tabita Naomi Ririmase, Pieter M. Ririmasse, Pieter Melianus Saiklela, Desy Megi Sarfan, Riri Sitorus, Jojor Desi Sulaiman, Kitriyani Tatipikalawan, Jomima M Tatipikalawan, Jomima Martha Tomatala, George Semuel Johny Tulalessy, Abraham Yulia Astri Kusuma Yulnawati .