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PERBEDAAN HASIL UJI KOAGULASE MENGGUNAKAN PLASMA SITRAT MANUSIA 3,8%, PLASMA SITRAT DOMBA 3,8%, DAN PLASMA SITRAT KELINCI 3,8% PADA BAKTERI Staphylococcus aureus Yunan Jiwintarum; Lalu Srigede; Auliya Rahmawati
Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Coagulase test is a bacterial examination performed to detect the formation of coagulase enzyme bound to the bacterial cell wall. This study aims to determine the difference in the form of a coagulase test results clot formation by using several types of plasma citrate in bacteria Staphylococcus aureus. This type of research is true experimental replication using 9 and 3 human citrate plasma treatment is 3.8%, 3.8% sheep plasma citrate, and 3.8% rabbit citrate plasma. The results show the average time of the formation of clots in the coagulase test using 3.8% human citrate plasma was 1:29 minutes, using sheep plasma citrate 3.8% was 00:54 minutes, and using 3.8% rabbit citrate plasma is 1:40 minutes. Results of One Way Anova statistical test obtained p value count (0,00) <p α (0.05), meaning that there are significant differences in the results of coagulase test using human plasma citrate 3.8%, 3.8% sheep plasma citrate, and rabbit plasma citrate 3.8% in Staphylococcus aureus.

Utilization Of Kuku Pancar (Lawsonia Inermis Lin) Leaves as a Substitute For Eosin Dye in The Examination of Soil Transmitted Helminth (STH) Eggs Lala Deswinta Yusari; Lalu Srigede; Thomas Tandi Manu; Ari Khusuma
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.356

Nail surfacing leaves (Lawsonia inermis lin) are natural materials commonly used as dyes, because of the content of chemical compounds in them such as lawsone, flavonoids, tannins and anthocyanins, therefore nail surfacing leaves can be used as an alternative dye to replace 2% Eosin in STH worm egg examination, because it has the same properties as 2% Eosin and can produce an orange yellow color after the extraction process. The research objective is to determine the results of nail surfacing leaves (Lawsonia inermis lin) can be used as an alternative substance to replace Eosin in the examination of STH (soil transmitted helminth) worm eggs. The methods of this research is using is experimental, where this study looks at the clarity of soil transmitted helminth (STH) worm eggs on preparations, using coloring from the results of soaking the leaves of the nail surf as a substitute for Eosin. The results of the research is that the coloring results obtained in each variation of immersion time are good. The conclusion of this research is that the staining of worm eggs using the results of immersion of nail surfacing leaves for 12 hours, 24 hours, 36 hours, and 48 hours can be used as an alternative dye in the examination of STH worm eggs.

Correlation of Blood Creatinine Levels with The Result of Urine Sediment Analysis in Chronic Kidney Failure Patient Siti Zaetun; Rohmi Rohmi; Andi Dwi Oktaviani; Lalu Srigede
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.339

Chronic Kidney Failure is a condition caused by a gradual and chronic decline in kidney function. This situation can affect to creatinine levels. Creatinine is the result of endogenous metabolism that is useful for assessing glomerular function, in the event of renal dysfunction then the filtration ability of creatinine will be reduced and serum creatinine will increase. Urine sediment is produced from the results of urine concentration consisting of calcium salts. This concentrated urine comes from the kidneys with a variety of causes, one of which is chronic kidney failure, flows through the ureter to the bladder and continues to the urethra until the urethral. Objective: To find out the correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure. Method: This research is observational analytical with a cross sectional approach to determine the correlation between independent and dependent variables. The sample is taken purposive sampling by selecting a sample based on certain criteria. The collected data is then analyzed using Spearman statistical tests. Results: Examination of creatinine levels in chronic kidney failure patients obtained 6.67 mg/dl. The average urine sediment found erythrocytes as many 2-3/HPF to many/HPF. Conclusion: There is a correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure.

Analisis Jenis dan Penyebab Anemia Berdasarkan Kadar Hemoglobin dan Nilai MCV (Mean Corpuscular Volume) Pada Penderita Tuberkulosis Paru Baru Di Puskesmas Dasan Tapen Nova Ramdhani, Iid Nirmala; Rohmi; Lalu Srigede; Agrijanti
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 2 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i2.106

Background: Tuberculosis is an infectious lung disease caused by Mycobacterium Tuberculosis which can cause several complications, including a decrease in hemoglobin levels, abnormal erythrocyte index values that can cause anemia. Examination of hemoglobin levels and MCV values to determine the type of anemia in tuberculosis patients. There are several types of anemia in tuberculosis patients and causes, one of which is microcytic anemia caused by a decrease in iron. Research Objective: Determine hemoglobin levels and erythrocyte index in patients with new pulmonary tuberculosis at Dasan Tapen Health Center Research Method: This study is observational analytic with Cross Sectional approach, the number of samples is 24 samples using the lomeslow formula with hemoglobin levels and MCV values. The collected data is then analyzed using descriptive tests. Results of the study: From 24 samples of tuberculosis patients as many as 5 people (21%) with a very mild degree of anemia, 16 (67%) people with a mild degree of anemia and 3 people (12%) with a moderate degree of anemia. A total of 18 people (75%) with microcytic type anemia and 6 (25%) people with normocytic type anemia. Conclusion: From 24 samples as many as 18 people (75%) with MCV values of <80 which indicates tuberculosis patients have microcytic anemia.

Efektivitas Cuka Tuak Aren (Arenga pinnata Merr.) sebagai Reagen Alternatif Pemeriksaan Protein Urin HUSNANINGSIH, ASPARIN; Lalu Srigede; Yudha Anggit Jiwantoro; Ida Bagus Rai Wiadnya
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 2 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i2.138

Background: Palm sap (Arenga pinnata Merr.) has an acid content that comes from fermentation in the form of alcohol, then turns into vinegar acid due to an increase in acidity by Acetobecter Acetic bacteria. This vinegar acid has the property of being a weak acid. The vinegar acid content in palm oil can be used as a reagent in urine protein examinations. The principle of urine protein examination is that urine protein is denatured by heating and acid addition. Research Objective: Determine the effectiveness of palm wine vinegar (Arenga pinnata Merr.) as an alternative reagent for urine protein examination. Research Method: This study is quasi-experimental and the sample in this study is protein positive urine with incidental sampling technique. The number of treatments was 3 treatments and replicated 9 times. Research Results: The results of urine protein examination using alternative reagents of palm wine vinegar (Arenga pinnata Merr.) with concentrations of 4%, 6% and 8% have similar results with examinations using 6% acetic acid solution as a control. Conclusion: Palm wine vinegar (Arenga pinnata Merr.) with concentrations of 4%, 6% and 8% is effectively used as an alternative reagent for urine protein examination. Keywords: Palm Vinegar, Urine protein, Acetic acid 6%.

Korelasi Derajat Keasaman dan Jenis Kristal Sedimen Urine pada Penderita Batu Saluran Kemih Wahyuni, Yeni; I Wayan Getas; Pancawati Ariami; Lalu Srigede
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 2 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i2.200

Latar Belakang: Batu saluran kemih merupakan masa keras yang ditemukan di sepanjang daerah saluran kemih. Pada penderita batu saluran kemih, pH urine sangat berperan dalam membentuk jenis batu saluran kemih. Apakah demikian kondisinya, maka dilakukan penelitian terkait pH urine dan jenis kristal pada sedimen urine. Tujuan: Untuk mengetahui korelasi derajat keasaman (pH) urine dan jenis kristal sedimen urine pada penderita batu saluran kemih. Metode: Rancangan penelitian menggunakan observasional analitik dengan pendekatan Cross Sectional. Jumlah sampel 27 orang dengan teknik pengambilan sampel menggunakan metode Accidental Sampling. Analisis data menggunakan uji statistik Chi Square. Hasil Penelitian: Pada sampel urine dengan pH 5.0 ditemukan jenis kristal kalsium oksalat dan amorf urat, pada pH 5.5 jenis kristal kalsium oksalat, asam urat dan amorf urat, pada pH 6.0 jenis kristal kalsium oksalat dan asam urat, pada pH 7.0-7.5 jenis kristal tripel fosfat, dan pH 8.0 jenis kristal asam urat dan tripel fosfat. Kesimpulan: Hasil uji statistik menunjukkan nilai Asymp.sig. 0,018 < 0,05, maka terdapat korelasi yang signifikan antara derajat keasaman (pH) urine dan jenis kristal sedimen urine pada penderita batu saluran kemih (BSK).

Hubungan Hasil Pemeriksaan Kultur Urin terhadap Hasil Protein dan Berat Jenis Urin sebagai Pemeriksaan Fungsi Ginjal pada Pasien Infeksi Saluran Kemih Supriyanti, Ispi; Agrijanti; Ari Khusuma; Lalu Srigede
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.146

Background : Laboratory examination for UTI consists of urine culture and dipstick urinalysis consisting of glucose, protein, bilirubin, urobilinogen, erythrocytes, pH, ketones, specific gravity, leukocytes and nitrites. In this study, only protein and urine specific gravity were examined, because one of the causes of proteinuria is post-renal which occurs due to infection in the urinary tract. Meanwhile, for urine specific gravity, patients who are suspected of having a urinary tract infection usually have a high urine specific gravity due to an increase in the number of leukocytes in the urine. UTI can cause a decrease in kidney function due to the risk of infection in the ureters which can cause several complications of kidney disease. Research Objective : To find out whether there is a relationship between the results of urine culture examination and the results of protein and specific gravity of urine as an examination of kidney function in UTI patients. Research Method : This research is an analytical observational research with a cross sectional research design. By examining urine culture and urinalysis using the dipstick method and analyzed using the chi square test. Results of the study : The results of data analysis showed a significant relationship in the positive direction with the strength of the relationship being quite strong between urine culture examination and urine protein results in UTI patients (p 0.030˂0.05). And there is a significant relationship in the positive direction with the strength of the relationship being quite strong between urine culture examination and urine specific gravity results in UTI patients (p 0.021<0.05). Conclusion : There is a significant relationship in the positive direction with the strength of the relationship being quite strong between the results of the protein examination and the urine culture results in UTI patients, as well as the results of the specific gravity examination and the strength of the urine culture results in UTI patients, there is a significant relationship in the positive direction with the strength of the relationship being quite strong.

Pengaruh Lama Senam terhadap Kadar Trigliserida pada Kelompok Program Pengelolaan Penyakit Kronis (PROLANIS) di Puskesmas Dasan Tapen Viefa Avrilian Alifah; Lale Budi Kusuma Dewi; Lalu Srigede; Ari Khusuma
Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 1 No. 2 (2025): Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : PT Pustaka Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71417/galen.v1i2.32

Sindrom metabolik ditandai oleh salah satunya kadar trigliserida tinggi yang meningkatkan risiko penyakit kardiovaskular. Aktivitas fisik seperti senam merupakan upaya non-farmakologis untuk menurunkan trigliserida. Program Pengelolaan Penyakit Kronis (prolanis) yang diselenggarakan oleh BPJS Kesehatan menyediakan kegiatan senam secara rutin sebagai bagian dari upaya pengendalian penyakit kronis. Namun, efektivitas senam prolanis terhadap kadar trigliserida masih belum banyak diteliti, terutama dalam kaitannya dengan lama partisipasi senam. Untuk mengetahui ada pengaruh lama senam terhadap kadar trigliserida pada kelompok program pengelolaan penyakit kronis (prolanis) di Puskesmas Dasan Tapen. Penelitian ini menggunakan desain observasional analitik dengan pendekatan cross-sectional. Sampel sebanyak 23 responden yang mengikuti senam setiap 1x setiap minggu dengan variasi lama partisipasi. Pemeriksaan trigliserida menggunakan metode enzimatik GPO-PAP, sedangkan data mengenai lama mengikuti senam diperoleh melalui kuesioner. Analisis data menggunakan uji Shapiro-Wilk, Anova, dan uji lanjutan Tukey HSD. Terdapat penurunan kadar trigliserida berdasarkan variasi lama bulan mengikuti senam. Hasil Anova menunjukkan perbedaan signifikan antar kelompok (p = 0,000 < 0,05). Kadar trigliserida tertinggi ditemukan pada bulan ke-3 yaitu 247,50 mg/dL, sedangkan kadar terendah pada bulan ke-9 yaitu 111,33 mg/dL. Lama mengikuti senam berpengaruh signifikan terhadap penurunan kadar trigliserida. Efek penurunan terlihat nyata setelah 6 bulan partisipasi rutin. Namun, faktor lain seperti pola makan, konsumsi obat-obatan, dan aktivitas fisik tambahan juga turut memengaruhi hasil.

Pengaruh Konsumsi Berbagai Jenis Sumber Karbohidrat terhadap Kadar Glukosa Darah 2 Jam PP pada Penderita Diabetes Melitus Tipe 2 Sufina Zikria Putri; Agrijanti; Thomas Tandi Manu; Lalu Srigede
Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 1 No. 2 (2025): Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : PT Pustaka Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71417/galen.v1i2.100

Latar Belakang: Diabetes Melitus tipe 2 merupakan salah satu masalah kesehatan yang terus meningkat di Indonesia. Salah satu faktor penting dalam pengelolaan diabetes adalah pengaturan konsumsi karbohidrat karena berpengaruh langsung terhadap kadar glukosa darah Postprandial (2 jam setelah makan). Jenis karbohidrat yang dikonsumsi diduga memiliki pengaruh yang berbeda terhadap respons glukosa darah penderita diabetes. Tujuan Penelitian: Mengetahui pengaruh konsumsi berbagai jenis sumber karbohidrat terhadap kadar glukosa darah 2 jam Postprandial pada penderita Diabetes Melitus tipe 2. Metode Penelitian: Penelitian ini merupakan penelitian kuantitatif dengan desain pre-eksperimen (pre and post test group design). Subjek penelitian terdiri dari 28 orang penderita Diabetes Melitus tipe 2 yang dibagi ke dalam 7 kelompok berdasarkan jenis karbohidrat yang dikonsumsi (beras merah, kentang, singkong, ubi talas, ubi jalar, jagung, dan nasi putih). Data dianalisis menggunakan uji Paired t-Test. Hasil Penelitian: Rata-rata kadar glukosa darah sebelum dan sesudah mengkonsumsi karbohidrat beras merah (434,5 dan 394,5 mg/dL), Kentang (337 dan 185,5 mg/dL), Singkong (447,25 dan 404,75 mg/dL), Ubi Talas (354,25 dan 305,5 mg/dL), Ubi Jalar (311 dan 257,25 mg/dL), Jagung (369 dan 353,75 mg/dL) dan nasi putih (293 dan 315,75 mg/dL). Uji Paired t-Test menunjukkan bahwa kelompok beras merah (p=0,001), kentang (p<0,001), ubi talas (p=0,023), ubi jalar (p=0,008) mengalami penurunan signifikan, sementara singkong dan jagung tidak signifikan (p>0,05). Nasi putih menunjukkan peningkatan signifikan (p=0,008). Kesimpulan: Terdapat pengaruh konsumsi beras merah, kentang, ubi talas dan ubi jalar menunjukkan penurunan signifikan terhadap kadar glukosa darah, sedangkan konsumsi singkong dan jagung mengalami penurunan yang tidak signifikan. Sementara itu, konsumsi nasi putih justru meningkatkan kadar glukosa darah secara signifikan.

Korelasi Jumlah Trombosit dengan Kadar Albumin pada Penderita Demam Berdarah Dengue (DBD) di Kota Mataram Rizani, Ulfiya; Lalu Srigede; Ariami, Pancawati; Kristinawati, Erna
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.61

Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus. DHF can cause two main pathological changes, namely increased capillary permeability, which causes hemoconcentration due to a decrease in plasma volume in the blood vessels and hemostasis disorders caused by coagulopathy, vasculopathy and thrombocytopenia. Routine examination parameters for dengue sufferers are complete blood to monitor platelet counts. Supporting examinations are also required, one of which is checking albumin levels. This research aims to know of determine the correlation between platelet counts and levels in dengue fever sufferers in Mataram City. This research uses the Observational Analytical method with a cross sectional approach. DHF sufferers will have a complete blood count and albumin examination, then an analysis will be carried out using the Spearman test. This study used 24 samples and it was found that the overall average platelet count was 56,000/ul of blood. The platelet value was obtained with a range of 26,000/ul blood to 91,000/ul blood. The overall average albumin level is 3.28 g/dL, with a range of albumin levels from 2.78 g/dL to 4.12 g/dL. There is a relationship between platelet count and albumin levels in dengue fever sufferers in Mataram City based on the results of the Spearman test with a p value <0.05.

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