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Analisis Hasil Pewarnaan Telur Cacing Menggunakan Pewarna Alternatif Filtrat Variasi Buah Muhammad Nasir; Rafika Rafika; Queen Cleverine; Zulfikar Ali Hasan; Nurdin Nurdin; M. Askar; Herman Herman
Jurnal Media Analis Kesehatan Vol 15 No 1 (2024): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v15i1.372

Abstract

Infeksi parasit pada manusia sering kali disebabkan oleh cacing nematoda usus yang penyebarannya melalui tanah, dikenal sebagai Soil Transmitted Helminths (STH). Penyakit ini dapat didiagnosis dengan pemeriksaan mikroskopis menggunakan pewarna Eosin 2%. Beberapa bahan alami yang dapat digunakan sebagai pewarna alternatif untuk mewarnai telur cacing antara lain buah bit (Beta vulgaris L.), buah naga (Hylocereus polyrhizus), dan buah stroberi (Fragaria vesca) karena kandungan antosianinnya. Penelitian ini dilaksanakan pada tanggal 11-13 April 2023 di Laboratorium Parasitologi Poltekkes Kemenkes Makassar. Tujuannya adalah untuk mengetahui kemampuan buah bit, buah naga merah, dan buah stroberi dengan konsentrasi 1%, 2%, 3%, dan 100% sebagai pengganti Eosin 2% pada pemeriksaan telur cacing STH. Jenis penelitian ini adalah observasi laboratorik dengan teknik pengambilan sampel simple random sampling. Hasil penelitian menunjukkan bahwa pada konsentrasi 100%, buah bit (Beta vulgaris L.) memberikan lapangan pandang yang kontras, dengan telur cacing yang menyerap warna dan bagian telur yang terlihat jelas. Buah naga (Hylocereus polyrhizus) menghasilkan lapangan pandang yang kurang kontras, dengan telur cacing yang kurang menyerap warna dan bagian telur yang kurang terlihat jelas. Sementara itu, buah stroberi (Fragaria vesca) tidak cocok digunakan untuk pemeriksaan telur cacing. Penelitian ini menyimpulkan bahwa buah bit (Beta vulgaris L.) dan buah naga (Hylocereus polyrhizus) dapat digunakan sebagai pewarna alternatif dalam pemeriksaan telur cacing Soil Transmitted Helminths
Identifikasi Jamur Penyebab Onychomycosis Pada Kerokan Kuku Pekerja Bangunan Di Desa Bontokassi Kecamatan Parangloe Kabupaten Gowa Zulfikar Ali Hasan; Radiatul Adawiyah; Nurlia Naim; Zulfian Armah
Lontara Journal of Health Science and Technology Vol 3 No 1 (2022): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v3i1.272

Abstract

Onychomycosis is a disease caused by dermatophyte and non-dermatophyte fungi that can attack human nails. Work environment, personal hygiene of workers, age and length of work can increase the risk of Onychomycosis. This study aims to identify the fungi that cause Onychomycosis on the nails of construction workers. This research is descriptive observational and using purposive sampling technique. This study used 2 methods of examination, namely directly and indirectly using 20% KOH and planting on Sabouraud Dextrose Agar culture media. The results of the identification of 15 samples using 20% KOH, found 1 (6.67%) positive sample with the discovery of hyphae and spores while 14 (93.3%) other samples were negative. The results of the identification on Sabouraud Dextrose Agar media that had been incubated at 37 oC for 3-5 days, it was found that 15 (100%) samples were positive for fungal infection. The Non-Dermatophyte, namely the discovery of 6 samples (40%) of Aspergillus Sp fungi and 5 samples of Penicillium Sp fungi (33.33%) and the discovery of Dermatophyte fungi, namely Trichopyton sp fungi as many as 3 samples (20%) and Epidermophyton sp as much as 1 sample (6.67%).
Perbandingan Kualitas Trombocyte Concentrate Dari Platelet Rich Plasma Dan Platelet Apheresis Pada Jumlah Trombosit Dan Residu Leukosit Rafika, Rafika; Amelia, Dewicha; Naim, Nurlia; Hasan, Zulfikar Ali
Jurnal Analis Kesehatan Vol. 10 No. 1 (2021): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v10i1.2717

Abstract

Transfusi trombosit sebagai terapi utama bagi pasien dengan trombositopenia. Pasien dewasa, sekitar 6 unit TC-PRP dibutuhkan dosis tersebut setara dengan 1 unit platelet apheresis. Semakin banyak TC-PRP yang ditransfusikan maka akan semakin tinggi paparan residu leukosit yang diterima. Penelitian ini mengetahui perbandingan kualitas TC-PRP dan platelet apheresis dilihat dari jumlah trombosit dan residu leukosit. Jenis penelitian analitik observasional laboratorik di Instalasi Laboratorium Sentral RSUP Dr. Mohammad Hoesin Palembang. Sampel ditentukan secara purposive sampling sebanyak 40 sampel pada 2 subyek yang berbeda. Pada TC-PRP, sebesar 15% unit memiliki jumlah trombosit ≥60x10⁹ dengan nilai rata-rata 617x103/µL dan 100% unit memiliki residu leukosit ≤0,2x10⁹ dengan nilai rata-rata 0,223x103/µL. Pada platelet apheresis, sebesar 100% unit memiliki jumlah trombosit ≥2,0x10¹¹ dengan nilai rata-rata 859x103/µL dan residu leukosit ≤0,3x10⁹ dengan nilai rata-rata 0,009x103/µL. Berdasarkan Permenkes RI No.91/2015, jumlah trombosit pada TC-PRP tidak memenuhi persentase standar sedangkan residu leukosit memenuhi standar yang direkomendasikan. Pada platelet apheresis, kedua parameter memenuhi standar yang direkomendasikan. Secara statistik diperoleh nilai p = 0,003 bahwa ada perbedaan pada jumlah trombosit antara TC-PRP dan platelet apheresis, serta diperoleh nilai p = 0,000 berarti ada perbedaan yang pada jumlah residu leukosit antara TC-PRP dan platelet apheresis. Platelet apheresis memiliki kualitas lebih baik daripada TC-PRP
Bahasa Inggris Ronny, Ronny; Hasan, Zulfikar Ali; Telan, Albina Bare
Jurnal Kesehatan Manarang Vol 10 No 3 (2024): December 2024
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v10i3.1781

Abstract

Efforts to meet clean water needs, especially for drinking water, depend on the condition of groundwater that is healthy and sufficiently available. Filtration is the process of separating particles from a liquid by passing the liquid through a permeable material. This study examines the Gravity-Fed Filtering System with innovative Imhoff technology, combined with a Primary Treatment stage, to produce clearer and higher-quality water. The objective of this research is to assess the effectiveness of this system in reducing contamination levels in groundwater, including iron (Fe), manganese (Mn), total hardness (Ca and Mg), and organic compounds. The study employed an experimental method with a quantitative approach and used a One Group Pretest-Posttest design. The research was conducted in two locations: Barombong Village in Makassar and Pauwo Village in Gorontalo. Fifteen samples were randomly selected for analysis. The study began with preliminary observations and initial testing in March 2022, followed by the main research and prototype evaluation in 2023-2024. The results show that the system effectively reduced Fe, Mn, organic compounds, and total hardness levels. In Makassar, the highest reduction in Fe is 87.3% in sample 8, while in Gorontalo, the highest reduction is 93.3% in sample 8. The highest reduction in manganese (Mn) in Makassar is 63.3% in sample 1, and in Gorontalo, it is 62.1% in sample 1. The highest reduction in organic compounds in Makassar is 81.6% in sample 3, while the lowest reduction in total hardness in Makassar is 77.1% in samples 4, 5, 6, and 10. In Gorontalo, the highest reduction in total hardness is 90.3% in samples 1, 2, and 3. Recommendations for the community, The use of gravity-fed filtering system technology as one of the media used to reduce iron and manganese concentrations is considered quite good, but for similar research to be carried out, modifications should be made to the media specifically starting with the size, shape and other variables that support it so that it is more effective in reducing pollutant concentrations.
Identifikasi Jamur Penyebab Onychomycosis Pada Kerokan Kuku Pekerja Bangunan Di Desa Bontokassi Kecamatan Parangloe Kabupaten Gowa Hasan, Zulfikar Ali; Adawiyah, Radiatul; Naim, Nurlia; Armah, Zulfian
Lontara Journal of Health Science and Technology Vol. 3 No. 1 (2022): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v3i1.272

Abstract

Onychomycosis is a disease caused by dermatophyte and non-dermatophyte fungi that can attack human nails. Work environment, personal hygiene of workers, age and length of work can increase the risk of Onychomycosis. This study aims to identify the fungi that cause Onychomycosis on the nails of construction workers. This research is descriptive observational and using purposive sampling technique. This study used 2 methods of examination, namely directly and indirectly using 20% KOH and planting on Sabouraud Dextrose Agar culture media. The results of the identification of 15 samples using 20% KOH, found 1 (6.67%) positive sample with the discovery of hyphae and spores while 14 (93.3%) other samples were negative. The results of the identification on Sabouraud Dextrose Agar media that had been incubated at 37 oC for 3-5 days, it was found that 15 (100%) samples were positive for fungal infection. The Non-Dermatophyte, namely the discovery of 6 samples (40%) of Aspergillus Sp fungi and 5 samples of Penicillium Sp fungi (33.33%) and the discovery of Dermatophyte fungi, namely Trichopyton sp fungi as many as 3 samples (20%) and Epidermophyton sp as much as 1 sample (6.67%).
Optimalisasi Ubi Jalar Putih (Ipomoea Batatas L.) Sebagai Media Alternatif Pengganti PDA untuk Pertumbuhan Aspergillus flavus Pratama, Ridho; Khatimah, Husnul; Adam, Alfira; Rafika, Rafika; Armah, Zulfian; Artati, Artati; Hasan, Zulfikar Ali
Jurnal Media Analis Kesehatan Vol 16 No 1 (2025): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Potekkes Kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/jmak.v16i1.1434

Abstract

Jamur Aspergillus flavus merupakan salah satu jenis kapang yang memiliki peran penting dalam berbagai bidang, termasuk industri pangan dan kesehatan. Media pertumbuhan jamur secara umum menggunakan Potato Dextrose Agar (PDA), namun biaya dan ketersediaan media ini dapat menjadi kendala dalam penelitian skala besar. Penelitian ini bertujuan untuk mengevaluasi efektivitas ubi jalar putih sebagai media alternatif untuk pertumbuhan Aspergillus flavus dibandingkan dengan media PDA komersial. Hasil penelitian menunjukkan bahwa Aspergillus flavus dapat tumbuh secara optimal pada media alternatif berbasis ubi jalar putih dengan karakteristik pertumbuhan yang serupa dengan media PDA. Faktor-faktor seperti kandungan karbohidrat, pH, suhu, dan kelembaban berperan penting dalam mendukung pertumbuhan jamur. Tidak ditemukan perbedaan yang signifikan antara media PDA dan media alternatif dalam mendukung pertumbuhan Aspergillus flavus. Kesimpulan dari penelitian ini adalah ubi jalar putih memiliki potensi sebagai media alternatif yang efektif untuk pertumbuhan Aspergillus flavus, terutama dalam kondisi penelitian yang memerlukan bahan yang lebih ekonomis dan mudah diperoleh.
Innovative Gravity-Fed Filtration System to Improve Coastal Community Water Quality Ronny, Ronny; Hasan, Zulfikar Ali; Telan, Albina Bare
Jurnal Kesehatan Manarang Vol 10 No 3 (2024): Bahasa Inggris
Publisher : Politeknik Kesehatan Kemenkes Mamuju

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33490/jkm.v10i3.1781

Abstract

Efforts to meet clean water needs, especially for drinking water, depend on the condition of groundwater that is healthy and sufficiently available. Filtration is the process of separating particles from a liquid by passing the liquid through a permeable material. This study examines the Gravity-Fed Filtering System with innovative Imhoff technology, combined with a Primary Treatment stage, to produce clearer and higher-quality water. The objective of this research is to assess the effectiveness of this system in reducing contamination levels in groundwater, including iron (Fe), manganese (Mn), total hardness (Ca and Mg), and organic compounds. The study employed an experimental method with a quantitative approach and used a One Group Pretest-Posttest design. The research was conducted in two locations: Barombong Village in Makassar and Pauwo Village in Gorontalo. Fifteen samples were randomly selected for analysis. The study began with preliminary observations and initial testing in March 2022, followed by the main research and prototype evaluation in 2023-2024. The results show that the system effectively reduced Fe, Mn, organic compounds, and total hardness levels. In Makassar, the highest reduction in Fe is 87.3% in sample 8, while in Gorontalo, the highest reduction is 93.3% in sample 8. The highest reduction in manganese (Mn) in Makassar is 63.3% in sample 1, and in Gorontalo, it is 62.1% in sample 1. The highest reduction in organic compounds in Makassar is 81.6% in sample 3, while the lowest reduction in total hardness in Makassar is 77.1% in samples 4, 5, 6, and 10. In Gorontalo, the highest reduction in total hardness is 90.3% in samples 1, 2, and 3. Recommendations for the community, The use of gravity-fed filtering system technology as one of the media used to reduce iron and manganese concentrations is considered quite good, but for similar research to be carried out, modifications should be made to the media specifically starting with the size, shape and other variables that support it so that it is more effective in reducing pollutant concentrations.