Article Per Year (5 Year)
p-Index From 2021 - 2026
1.066
P-Index
This Author published in this journals
All Journal Microbiology Indonesia JURNAL ISTEK Majalah Kedokteran BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Makara Journal of Science Biodiversitas Journal of Biological Diversity
WELLYZAR SJAMSURIDZAL
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Medicine & Pharmacology

Published : 24 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 24 Documents
Search

 1   2   3 
Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area MAZYTHA KINANTI RACHMANIA; FITRIA NINGSIH; PUTRI PRATIWI SETYANINGSIH; WINDA AYU SYAFITRI; DHIAN CHITRA AYU FITRIA SARI; SHUHEI YABE; AKIRA YOKOTA; ARIYANTI OETARI; Wellyzar Sjamsuridzal
Biodiversitas Journal of Biological Diversity Vol. 21 No. 1 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210119

Abstract

Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.
Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella Fitria Ningsih; Dhian Chitra Ayu Fitria Sari; Shuhei Yabe; Akira Yokota; Wellyzar Sjamsuridzal
Biodiversitas Journal of Biological Diversity Vol. 21 No. 12 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211225

Abstract

Abstract. Ningsih F, Sari DCAF, Yabe S, Yokota A, Sjamsuridzal W. 2020. Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella. Biodiversitas 21: 5674-5684. Microbial resistance to available antibiotics has gained increasing attention in recent years and led to the urgent search for active secondary metabolites from novel microbial taxa. This study aimed to assess putative secondary metabolite biosynthetic gene clusters (BGCs) in the genome of a novel thermophilic Actinobacteria type strain Gandjariella thermophila SL3-2-4T and screen for its antibacterial activity. Four other related novel candidate Actinobacteria strains, isolated from forest soil in the Cisolok geothermal area (West Java, Indonesia), were also screened for antibacterial activity in various media solidified with gellan gum. The genome of the SL3-2-4T strain contained 21 antiSMASH-identified secondary metabolite regions harboring BGCs. These BGCs were for polyketide synthase, non-ribosomal peptide synthase, and ribosomally synthesized and post-translationally modified peptide family clusters. Three BGC regions displayed 50-100% similarity with known secondary metabolites. Thirteen and five regions displayed low (4-35%) and no similarity with known BGCs for secondary metabolites, respectively. Strains SL3-2-4T and SL3-2-7 on MM 2 medium solidified with gellan gum at 45 °C for 14 days demonstrated inhibitory activity against all Gram-positive, but not Gram-negative bacteria. Strain SL3-2-10 on ISP 3 gellan gum medium incubated for seven days only active against K. rhizophila NBRC 12078. Strains SL3-2-6 and SL3-2-9 did not exhibit any antibacterial activity against the tested bacterial strains on the three tested media. The results indicated that novel taxa have the potential for the discovery of active secondary metabolites.
Identification and screening of enzymatic abilities of Ktedonobacteria from forest soil of Cisolok Geothermal Area, Indonesia MAZYTHA KINANTI RACHMANIA; FITRIA NINGSIH; DHIAN CHITRA AYU FITRIA SARI; YURIZA ESHANANDA; YASUTERU SAKAI; SHUHEI YABE; AKIRA YOKOTA; WELLYZAR SJAMSURIDZAL
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230935

Abstract

Abstract. Rachmania MK, Ningsih F, Sari DCAF, Eshananda Y, Sakai Y, Yabe S, Yokota A, Sjamsuridzal W. 2022. Identification and screening of enzymatic abilities of Ktedonobacteria from forest soil of Cisolok Geothermal Area, Indonesia. Biodiversitas 23: 4686-4695. This study aimed to provide information on culturable Ktedonobacteria from forest soil in the Cisolok geothermal area and their potential as enzyme producers. Twelve ktedonobacterial isolates were obtained from this study and identified based on full-sequence of the 16S rRNA gene. Seventeen isolates (including five isolates from previous studies) were used for enzymatic screening and phylogenetic analyses. Screening of amylolytic (0.5% soluble starch) and cellulolytic (0.5% carboxymethylcellulose) (CMC) activities from ktedonobacterial isolates was performed on a ten-fold diluted R2A agar medium, and were incubated at 30 °C for 21 days. The EzBioCloud search revealed that all isolates showed low sequence similarities with Dictyobacter aurantiacus S-27T (97.82 to 98.18%) as their closest related species. The phylogenetic tree showed that all isolates belong to the genus Dictyobacter (family Dictyobacteraceae of the class Ktedonobacteria). All isolates formed a monophyletic group and were placed as the sister clade to D. aurantiacus, as supported by a very strong bootstrap value (99%). Screening of amylolytic and cellulolytic abilities showed that most isolates (88.23%) were able to degrade both 0.5% starch and 0.5% CMC as substrates. This study revealed the presence of Ktedonobacteria with amylolytic and cellulolytic abilities in the forest soil of Cisolok geothermal area.
Characterization and detached-leaf pathogenicity of representative pestalotioid fungal isolates from symptomatic rubber leaves in selected areas of Sumatra, Indonesia FARRIZA DIYASTI; WEN DEE ONG; TSABITAH SITI RAHMA; ACHMAD EKA SATRIA; TRI RAPANI FEBBIYANTI; STEPHANY ANGELIA TUMEWU; FITRINE EKAWASTI; MINAMI MATSUI; NEO ENDRA LELANA; WELLYZAR SJAMSURIDZAL; RETNO LESTARI
Biodiversitas Journal of Biological Diversity Vol. 27 No. 8 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270809

Abstract

Abstract. Diyasti F, Ong WD, Satria AE, Febbiyanti TR, Tumewu SA, Ekawasti F, Matsui M, Lelana NE, Sjamsuridzal W, Lestari R. 2026. Characterization and detached-leaf pathogenicity of representative pestalotioid fungal isolates from symptomatic rubber leaves in selected areas of Sumatra, Indonesia. Biodiversitas 27 (8): d270809. https://doi.org/10.13057/biodiv/d270809. Pestalotioid fungi have emerged as important pathogens associated with rubber leaf fall disease in Indonesia. This study characterized the morphological traits, molecular identity, and pathogenicity of five representative pestalotioid fungal isolates, each selected from a different sampling location across four major rubber-producing provinces in Sumatra (Riau, Jambi, South Sumatra, and Bangka Belitung). Five representative isolates (P_R.SS1, P_R.JM, P_R.BB, P_R.SS2, and P_R.RA) were selected for detailed morphological, molecular, and pathogenicity analyses. Morphological observations revealed variation in colony color, growth rate, and conidial dimensions. Molecular identification based on Internal Transcribed Spacer (ITS) rDNA sequences assigned the isolates to the genera Neopestalotiopsis and Pseudopestalotiopsis. Pathogenicity testing using a detached-leaf assay on Hevea brasiliensis clone PB260 revealed significant differences in lesion development among the five representative isolates at 7 days post-inoculation. Isolate P_R.RA from Riau produced the largest necrotic lesion area (0.802±0.332 cm²) despite exhibiting the slowest in vitro colony growth rate (35.807±1.56 cm²), whereas isolates P_R.SS1, P_R.JM, and P_R.BB produced lesion areas below 0.06 cm². These results indicate differences in pathogenicity among the representative isolates examined in this study. These findings provide preliminary baseline information for future multilocus identification and broader investigations of pestalotioid fungi associated with rubber leaf fall disease.
Co-Authors . RIDAWATI ACHMAD EKA SATRIA Adi Basukriadi AKIRA YOKOTA AKIRA YOKOTA AKIRA YOKOTA Akira Yokota Andi Salamah ANDO KATSUHIKO ANDRIA AGUSTA ANDRIA AGUSTA Anggita R Hafsari Arif Nurkanto ARIF NURKANTO ARIYANTI OETARI ARIYANTI OETARI Ariyanti Oetari Ariyanti Oetari ARIYANTI OETARI ARIYANTI OETARI Atit Kanti BANGGA BERISTAMA PUTRA Betty Sri Laksmi Jenie BETTY SRI LAKSMI JENIE Betty Sri Laksmi Jenie CAHYA GUSLYANI CHIHARU NAKASHIMA DHIAN CHITRA AYU FITRIA SARI DHIAN CHITRA AYU FITRIA SARI DHIAN CHITRA AYU FITRIA SARI DHIAN CHITRA AYU FITRIA SARI Dhian Chitra Ayu Fitria Sari Endah Wati Zahroh Etty Riani FARRIZA DIYASTI Fitria Ningsih fitria ningsih FITRINE EKAWASTI Floreta Fiska Yuliarni Gatot F Hertono HEDDY JULISTIONO Heddy Julistiono Hertono, Gatot Fatwanto IIN SITI DJUNAIDAH Iman Hidayat Iman Hidayat Imran, Darma Indrawati Gandjar Ita Djuwita ITA DJUWITA ITA DJUWITA Machrumnizar Machrumnizar, Machrumnizar MAZYTHA KINANTI RACHMANIA MINAMI MATSUI Muslim, Mohammad Natriana, Tjahjani NEO ENDRA LELANA Nining Betawati Prihantini PUTRI PRATIWI SETYANINGSIH PUTRI PRATIWI SETYANINGSIH PUTRI PRATIWI SETYANINGSIH RASTI SARASWATI Ratna Yuniati RETNO LESTARI Retno Wahyuningsih Retno Wahyuningsih Ridawati . Ridawati, Ridawati Robiatul Adawiyah Sari, Dhian Chitra Ayu Fitria SHUHEI YABE SHUHEI YABE SHUHEI YABE Shuhei Yabe SILVA ABRAHAM Sitaresmi . Sitaresmi, Sitaresmi STEPHANY ANGELIA TUMEWU Suyanto Pawiroharsono TRI RAPANI FEBBIYANTI TSABITAH SITI RAHMA TUBAGUS HAERU RAHAYU WEN DEE ONG WINDA AYU SYAFITRI WINDA AYU SYAFITRI WINDA AYU SYAFITRI Yantyati Widyastuti YASUTERU SAKAI Yokota, Akira Yuliarni, Floreta Fiska YURIZA ESHANANDA