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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter The Journal of Experimental Life Sciences (JELS) Biotropika Natural B The Journal of Pure and Applied Chemistry Research Molecular and Cellular Biomedical Sciences (MCBS)
Aris Soewondo
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University, Malang, 65145, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Veterinary Other

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Journal : Natural B

 1 
Allergenity Test of Porang Bulb Variant (Amorphophallus oncophyllus Hook.) By ELISA Ig-E Method Sofy Permana; Sri Widyarti; Serafinah Indriyani; Dian Siswanto; Aris Soewondo
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.475 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.14

Abstract

Porang (Amorphophalus oncophyllus Hook.), a tuber plant, is intensively explored for export commodities from East Java and as a raw food stuff material for domestic industry. The prospects of Porang tubers as the healthy food is interesting, however some studies are needed for their allergy effects. It was thought that the allergy effects of Porang are caused by the composition of Calcium Oxalate crystal that leads the itching and irritation in the mouth. On the other hands, the food allergy is usually caused by unchanged protein during food digesting.  Some proteins have been known that play a role in Calcium Oxalate crystal (Matrix proteins) formation, they are: Asp-rich and Ser-rich acidic proteins that are tightly bound to Calcium. So, substantially the allergen of Porang tuber is from the matrix protein, not from its crystal. The allergen in the food is usually a protein that is not change during the food digestion. In this study, it has been done the allergic test of some Porang tuber variants, namely: Sumber Baru-Jember; Klangon, Saradan-Madiun and Sumber Bendo, Saradan-Madiun, by ELISA-IgE from rat’s blood serum that have been orally treated by raw Porang tubers powder before.  The SDS-PAGE analysis shows that the proteins bands profiles are similar between raw and steamed Porang tubers (appearance Molecular Weight: 18 and 15 kDa). But, those raw Porang tubers bands are thicker than the steamed ones. The thickness of those raw Porang tubers decrease from 25 to 50 %, compare to those steamed ones. It means that the steaming of Porang is effective to decrease the potential allergy of Porang for consumptions. The allergic test analysis by ELISA-IgE revealed that the potential allergy of Porang are: Sumber Baru (Jember) > Porang var. Klangon - Saradan (Madiun) > Porang var. Sumber Bendo - Saradan (Madiun), respectively.
Co-Authors Agung Pramana W. M. Agung Pramana W.M Agung Pramana W.M Agung Pramana W.M Agung Pramana Warih Marhendra Agung Pramana Warih Marhendra Ahmad Shobrun Jamil Al Faizah, Belinda Nabiila Anaqoh Roudhotul Jannah Winarso Anzila, Ivakhul Ardiansyah, Esha Asyhari, Firda Nuri Atho'illah, Mochammad Fitri Dian Siswanto Dliyauddin, Moh Fadlilah, Dawama Nur Farida Rachmawati, Farida fatchiyah . Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fikriya Novita Sari Fikriya Novita Sari Firstiantono, Andista Fitria Eka Aprilia HUDA, ZAINUL Iskandar, Rossdannisa Isna Hapsari, Ajeng Hanum Ivakhul Anzila Izati, Rahmi Kavitarna, Septhyanti Aprilia Khairatul Insani Febrianti Khairatul Insani Febrianti Laili Nur Zaidah Mansur Ibrahim Moch Sasmito Djati Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa’i Muhaimin Rifa’i Muhammad Halim Natsir Muhammad Halim Natsir Nashi Widodo Nurul Shafa Paramita Putri, Nenis Try Melani Rifa'i, Muhaimin Rifa’i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Dwi Prasetiani Ririn Dwi Prasetiani, Ririn Dwi Rivqi Rifa Bia Rizky Senna Samoedra Rizky Senna Samoedra Robitoh Desi Kurniasari Rohmah, Meisya Putri Maulida Sa'adah, Nur Alfi Maghfirotus Sakti, Muhammad Wisam Wira Sakti, Sefihara Paramitha Sari, Fikriya Novita Savira Ayunda Rakhmanira Serafinah Indriyani Setyaki Kevin Pratama Setyaki Kevin Pratama Shinta Dwi Riestanti Shinta Dwi Riestanti, Shinta Dwi Shinta Oktya Wardhani, Shinta Oktya Siti Nur Arifah, Siti Nur Sofy Permana Sri Rahayu Sri Rahayu SRI RAHAYU Sri Rahayu Sri Widyarti Triandari, Ade W. M., Agung Pramana Warih Marhendra, Agung Pramana Wira Eka Putra Wisnu Barlianto YOGA DWI JATMIKO Yuyun Indriani Kartiko Santi Zulfatim, Heni Sukma