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Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment Suprihatin, Teguh; Widyarti, Sri; Rifa'i, Muhaimin; Rahayu, Sri
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

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Premenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausal white rat blood after oral turmeric powder treatment. The animals used were 30 female Wistar strains white rat, age 12 months with an average body weight 200-250 g. The animals were divided into 6 groups, namely the negative control group (P0) with 4 ml/day distilled water treatment; positive control group (P1), this group was treated with pure curcumin powder 6.75 mg/kg BW; treatment group 1 (P2), group was treated with turmeric powder 100 mg/kg BW; treatment group 2 (P3); treatment group 3 (P4); and treatment group 4 (P5), these group were treated with turmeric powder at 200 mg/kg BW; 400 mg/kg BW; and 800 mg/kg BW dose respectively. Oral Treatment was administered daily for 27 days. Blood collection was performed on days 0, 14, and 28. The ovarian collection was conducted on day 28. MDA ovarian level was measured using TBA method and blood serum estradiol level was measured using ELISA method. The results exhibited that the positive control group (P1) and the treatment group (P2-P5) showed significantly lower ovarian MDA levels compared with the negative control group (P0). The turmeric powder dose 200 mg/kg BW (P3) can increase estradiol levels by day 14 (3.32 Â± 0.26 Ïg/mL) and at day 28 (4.01 Â± 0.26 Ïg/mL).

The Effect of Calcusolâ„¢ to the Plasma Free Radical and Serum Creatinin in Mus Musculus Nephrolithiasis Model Rosyidah, Aâ€™liyatur; Widyarti, Sri; Rahayu, Sri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.%x.%x.%x

Effect of Dietary Amorphophallus sp From East Java on LDL-C Rats (Rattus novergicus Wistar Strain) Harijati, Nunung; Widyarti, Sri; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 1, No 2 (2011)
Publisher : Journal of Tropical Life Science

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One of indication of obesity is high LDL-C . Obesity has serious risk to health, it can cause heart disease and stroke. Effort to lower obesity using drugs have significant side effects such as insomnia, increased blood pressure, dry mouth and so forth. Therefore using natural products that contain glucomannan to reduce obesity and LDL-C is good choise. Glucomannan in the global market derived from Amorphophallus konjac. In this study, we used glucomannan from Amorphophallus endemic East Java to reduce fattened Wistar rats. Amorphophallus that used include Amorphophallus muelleri, A.variabilis (variant: Brongkos 32; Brongkos 5; Wonorejo, Brangsi) and Amorphophallus campanulatus (Var Selopuro). Amorphophallus diet was given to white rats of Wistar strain that previously fattened using a mixture of cholesterol and lard, in addition to PARS (Chicken Feed Race Super) as a basic food. Amorphophallus konjac was used as control. Measurement of LDL-C was conducted in four different time points, namely 1). Prior to dietary cholesterol, 2). 24 days after the dietary cholesterol, 3) 11 days after Amorphophallus (+cholesterol) diet, 4). 25 days after Amorphophallus (+cholesterol) diet. The results showed that the diet of A.variabilis potentially lowered blood cholesterol levels for their respective 22.98%, 5.85% and 7.37% for consecutive variant Brongkos 32; Brongkos 5; Wonorejo. Diet from A.campanulatus and A.konjac had not been able to reduce cholesterol to the end of observation (25 days).Keywords: LDL-C, A.variabilis, A.muelleri, A.campanulatus, A.konjac

The Effect of Different Orthodontic Force on MMP 9 Expression in a Rat Diabetic Model Maulana, Hafiedz; Hikmah, Nuzulul; Shita, Amandia Dewi permana; Permatasari, Nur; Widyarti, Sri
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Orthodontic forces produce alveolar bone and periodontal ligament remodeling that cause tooth movement. Diabetes can increase tissue damage and, therefore, contributes to the process of orthodontic tooth movement. The aim of this study was to determine the effect of differently orthodontic forces on the expression of MMP nine in rat diabetic model. Twenty four male Wistar rats were divided into three groups of control and three groups of treatment streptozotocin-induced diabetes with stratified doses. Application of orthodontic appliance was performed with different forces (10, 20 and 30 grF). Orthodontic appliance was performed on both upper incisors. Immunohistochemical (IHC) analysis was used to observe the expression of MMP 9 and HE (Hematoxylen Eosin) staining to observe the number of fibroblasts. Statistical analysis was carried out using t-test and Spearman correlation analysis to determine the difference of MMP nine expression and number of fibroblasts between groups, and determine the correlation of both. The results showed an increased expression of MMP 9 and decreased of fibroblasts number in diabetic rats, along with increasing magnitude of orthodontic forces. The different orthodontic forces given to diabetic rats affect the expression of MMP 9 and the number of fibroblasts, in which the expression of MMP 9 increased along with the increase of orthodontic forces, both at pressure and tension sides.

Dose Optimization of Calcusolâ„¢ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice (Mus musculus) Soni, Ahmad; Djati, Muhammad Sasmito; Widyarti, Sri
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Kidney stones are one of the urologic diseases that have plagued mankind for centuries. The main constituents of stones in the kidney are calcium oxalate monohydrate (COM) crystals. Nowadays, there are varieties of drugs and treatments that can be made to minimize the grievances due to kidney stone disease. The treatment can be done either by using chemicals or traditional medicine. Calcusolâ„¢ is one of the popular herbal products that have been used by Indonesian people in curing the kidney stone disease. The main constituent that was contained in Calcusolâ„¢ is an extract of the tempuyung leaves (Sonchus arvensis L.), which is expected could cure the kidney stone disease. This study used primary cultured renal epithelial cells of mice to determine the optimal dose of Calcusol and the optimal dose of COM. The primary Kidney epithelial cell were treated with Calcusolâ„¢ and COM at various doses. The analysis of the cell death either by necrosis or apoptosis pathways was analyzed by flow cytometric analysis. The results that were obtained is the percentage of cell death that is then analyzed by using a complete randomized design (CRD) One Way Anova. Based on the results that were obtained, it is known that the optimal dose of Calcusolâ„¢ in vitro were ranging from 75 ppm to 100 ppm, whereas the optimal dose of COM suggested for 500 ppm.

Formaldehyde And Benzo(Î‘)Pyrene induced Cells Necrotic in Mice Liver Soni, Ahmad; Widyarti, sri; Suwondo, Aris
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Formaldehyde and benzo(α)pyrene are compounds that harmful for health. Misapplication of this compound have an impact in the form of organ damage in the body. This study aims to determine the impact of the treatment of the combined exposure of formaldehyde and benzo(α)pyrene to cell necrosis in the liver of mice (Mus musculus). Treatment of formaldehyde dose of 25 mg/kg BW to mice were given â€‹â€‹orally every day for 60 days. Treatment of benzo(α)pyrene via intraperitoneal injection at a dose of 250 mg/kg BW were given after 30 days of incubation with four times injection with one day interval. Liver organ histological preparations were made through the HE staining. Observations were made by using a microscope for liver organ preparations. The data obtained that is the percentage of cells necrosis and necrotic foci. This research used Completely Randomized Design (CRD) with 95% confidence interval. Liver organ preparations observations indicate that the percentage of necrosis in the untreated control, benzo(α)pyrene 250 mg/kg BW, Formaldehyde 25 mg/kg BW, combination of formaldehyde 25 mg/kg BW with BaP in a row that is equal to 14.43% ± 0.91; 26.05% ± 3.75; 49.38% ± 2.66; 51.86 ± 1.73. The mean of necrotic foci in liver organ formed in the untreatment control, benzo(α)pyrene 250 mg/kg BW, Formaldehyde 25 mg/kg BW, and the combination of formaldehyde 25 mg/kg BW with BaP in a row, equal to 1.3 ± 0,07; 1.63 ± 0.61; 2 ± 0.51, and 3.4 ± 0.76. This suggests that the combined treatment had the highest level of toxicity compared with other treatments.

Accumulation of Calcium Crystal in Rat (Rattus norvegicus) Kidney Rosidah, A'liyatur; Widyarti, Sri; Rahayu, Sri
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Effect of Dietary Amorphophallus sp From East Java on LDL-C Rats (Rattus novergicus Wistar Strain) Nunung Harijati; Sri Widyarti; Rodiyati Azrianingsih
Journal of Tropical Life Science Vol. 1 No. 2 (2011)
Publisher : Journal of Tropical Life Science

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One of indication of obesity is high LDL-C . Obesity has serious risk to health, it can cause heart disease and stroke. Effort to lower obesity using drugs have significant side effects such as insomnia, increased blood pressure, dry mouth and so forth. Therefore using natural products that contain glucomannan to reduce obesity and LDL-C is good choise. Glucomannan in the global market derived from Amorphophallus konjac. In this study, we used glucomannan from Amorphophallus endemic East Java to reduce fattened Wistar rats. Amorphophallus that used include Amorphophallus muelleri, A.variabilis (variant: Brongkos 32; Brongkos 5; Wonorejo, Brangsi) and Amorphophallus campanulatus (Var Selopuro). Amorphophallus diet was given to white rats of Wistar strain that previously fattened using a mixture of cholesterol and lard, in addition to PARS (Chicken Feed Race Super) as a basic food. Amorphophallus konjac was used as control. Measurement of LDL-C was conducted in four different time points, namely 1). Prior to dietary cholesterol, 2). 24 days after the dietary cholesterol, 3) 11 days after Amorphophallus (+cholesterol) diet, 4). 25 days after Amorphophallus (+cholesterol) diet. The results showed that the diet of A.variabilis potentially lowered blood cholesterol levels for their respective 22.98%, 5.85% and 7.37% for consecutive variant Brongkos 32; Brongkos 5; Wonorejo. Diet from A.campanulatus and A.konjac had not been able to reduce cholesterol to the end of observation (25 days).Keywords: LDL-C, A.variabilis, A.muelleri, A.campanulatus, A.konjac

Accumulation of Calcium Crystal in Rat (Rattus norvegicus) Kidney A'liyatur Rosidah; Sri Widyarti; Sri Rahayu
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

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The aim of this research is to know the effect of tuber Porang flour (Amorphophallus muelleri) to the accumulation of calcium crystal. This research uses 2,5 months old male rats that are orally given by tuber Porang flour from three different location (Klangon, Sumber Baru, and Sumber Bendo) with doses 6 mg/100 gr BW/day for 3 months. Kidneys taken to analyzed distribution of crystal calcium accumulation. Observation of distribution crystal calsium accumulation with microscope of Olympus CX 31 at 400x magnification. The data to be analysed by using test of ANOVA with SPSS 16 for windows. The result of research known that the treatment of tuber Porang flour induce calcium crystal accumulation in rat kidneys. Using tuber porang flour from different location can generate different impact at accumulation of crystal calcium. The lowest calcium crystal accumulation in kidneys with giving Klangon Porang flour that is equal to 8,19±2,17 spot/ area. Consumption of the tuber Porang flour cause the accumulation of crystal calcium in rat kidney.

Formaldehyde And Benzo(Î‘)Pyrene induced Cells Necrotic in Mice Liver Ahmad Soni; sri Widyarti; Aris Suwondo
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Formaldehyde and benzo(α)pyrene are compounds that harmful for health. Misapplication of this compound have an impact in the form of organ damage in the body. This study aims to determine the impact of the treatment of the combined exposure of formaldehyde and benzo(α)pyrene to cell necrosis in the liver of mice (Mus musculus). Treatment of formaldehyde dose of 25 mg/kg BW to mice were given â€‹â€‹orally every day for 60 days. Treatment of benzo(α)pyrene via intraperitoneal injection at a dose of 250 mg/kg BW were given after 30 days of incubation with four times injection with one day interval. Liver organ histological preparations were made through the HE staining. Observations were made by using a microscope for liver organ preparations. The data obtained that is the percentage of cells necrosis and necrotic foci. This research used Completely Randomized Design (CRD) with 95% confidence interval. Liver organ preparations observations indicate that the percentage of necrosis in the untreated control, benzo(α)pyrene 250 mg/kg BW, Formaldehyde 25 mg/kg BW, combination of formaldehyde 25 mg/kg BW with BaP in a row that is equal to 14.43% ± 0.91; 26.05% ± 3.75; 49.38% ± 2.66; 51.86 ± 1.73. The mean of necrotic foci in liver organ formed in the untreatment control, benzo(α)pyrene 250 mg/kg BW, Formaldehyde 25 mg/kg BW, and the combination of formaldehyde 25 mg/kg BW with BaP in a row, equal to 1.3 ± 0,07; 1.63 ± 0.61; 2 ± 0.51, and 3.4 ± 0.76. This suggests that the combined treatment had the highest level of toxicity compared with other treatments.

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