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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pendidikan Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Bioedukasi: Jurnal Pendidikan Biologi JPS Unimus BERITA BIOLOGI BIOMA Argipa (Arsip gizi dan Pangan) Jurnal Teknologi Pangan dan Hasil Pertanian AGRITEKNO, Jurnal Teknologi Pertanian TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Jurnal Komunikasi Pendidikan Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Riset Biologi dan Aplikasinya Proceeding Biology Education Conference Journal Of World Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) AGRIC Science Technology and Management Journal (STMJ) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Lusiawati Dewi
Satya Wacana Christian University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Jurnal Teknologi Dan Industri Pangan

 1 
AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI EKSTRAK POLAR DAN NON POLAR BIJI SELASIH (Ocimum sanctum Linn) [Antioxidant and Antibacterial Activities from Polar and Non Polar Basil (Ocimum sanctum Linn) Seed Extracts] Agustina D. R. Nurcahyanti; Lusiawati - Dewi; Kris H. Timotius
Jurnal Teknologi dan Industri Pangan Vol. 22 No. 1 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The aims of this study were to determine total phenolic content, antioxidant activity and antibacterial activity of polar and nonpolar extracts of basil (O. sanctum L.) seed. Seeds of basil (O. sanctum L.) were extracted using a soxhlet extractor using four types organic solvent, i.e. chloroform, ethyl acetate, acetone, and methanol for eight hours each. The total phenolic content was determined using Folin Ciocalteu method, antioxidant activity was determined using reducing power and DPPH (1,1-diphenyl,2-picrylhydrazyl) scavenging activity methods, while antibacterial activity was tested using agar diffusion method. The result showed that the highest total phenolic content and antioxidant activity was obtained in methanol extract with 3.63 ± 0.21 mgGAE/g phenolic total, 58.39 ± 3.81 mek/g using reducing power method and 85.73 ± 0.86% free radical scavenging activity. Furthermore the result of antibacterial activity testing showed that the highest diameter of inhibition zone was observed in ethyl acetate extract inhibition on E.coli, P.aeruginosa, B.subtilis, and S.aureus where its inhibition zone were 13.53 ± 0.63; 10.67 ± 1.05; 14.93 ± 0.80, and 13.46 ± 0.79 mm, respectively. This result suggests that both polar and nonpolar basil seed extracts possess specific biological activity. This data provide valuable and strong database for exploration of natural antibacterial agents and antioxidant for food and health industry application. 
Co-Authors Aditya Surya Piansti Agus Ariyanto Agustina Dwi Retno Nurcahyanti Andreas Binar Aji Sukmana Andriyani Dea Wulandari Anggada, Richard Dharma Anggara Mahardika Anggara Mahardika Anggara Mahardika Ardhikajaya Wahyu Prasetya Brigitta Erlien Mutiara Yuliastuti Desy Fajar Priyayi Dhanang Puspita Dwianto, Stefanus Agung Ega Meilenia Putri Pamungkas Ellent, Sifera S C Ferdy F Rondonuwu Gracelia, Kresentia D Hapsari, Martina Widhi Hartati Soetjipto Heleri, Heleri Khanifa Nurul Khaq Kris H. Timotius Kusuma, Rizky Dewi Darma Lelatobur, Lovely Ezverenzha Marampa, Edgar Meyer Marisa Christina Tapilouw Monica Ageng Wardani Novianingrum, Milka Putri Novianti, Riska T Novitasari Priskalia Puter Novitasari Priskalia Puteri Onesiforus O. Dhimas Asmara Prasetya, Adelia Purwoko, Agus Ratih Paramastuti Richard Dharma Anggada Risya Pramana Situmorang Rivy Valen Pabesak Rizkaprilisa, Windy Sentot Adi Prayitno Setiawan, Sia, Clarissa Eleora Sopacuaperu, Sonia Falensia Sucahyo Sucahyo, S Vincentia Irene Meitiniarti WINDY WINDY