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All Journal Jurnal Ilmu Pertanian VALENSI CHEMISTRY PROGRESS Jurnal Sains Dasar Alchemy : Journal of Chemistry Jurnal Ilmu Kimia Universitas Brawijaya Indonesian Green Technology Journal Natural B The Journal of Pure and Applied Chemistry Research Program Kreativitas Mahasiswa - Penelitian Majalah Kesehatan FKUB Indonesian Journal of Chemistry Jurnal Saintek Lahan Kering Molekul: Jurnal Ilmiah Kimia jurnal Ilmu Pertanian (Agricultural Science) JKPK (Jurnal Kimia dan Pendidikan Kimia) ALKIMIA : Jurnal Ilmu Kimia dan Terapan Jurnal Muara Sains, Teknologi, Kedokteran dan Ilmu Kesehatan Bubungan Tinggi: Jurnal Pengabdian Masyarakat Jurnal Ilmiah Berkala Sains dan Terapan Kimia Makara Journal of Science al Kimiya : Jurnal Ilmu Kimia dan Terapan
Hermin Sulistyarti
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Urine Glucose Detection Via Gold Nanoparticle Formation Using 3D-Connector Microfluidic Paper Based Analytical Devices Putri, Krista Firdaus Suwarno; Sulistyarti, Hermin; Sabarudin, Akhmad
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol. 11 No. 1 (2024): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v11i1.35245

Abstract

A metabolic disorders that have experienced a significant increase in the world are diabetes mellitus. Diabetes is caused by two main factors: the first is damage to pancreatic beta cells, which prevents insulin from being produced, and the second is impaired insulin secretion and function. Chronic diabetes, if not treated properly, can lead to acute complications including eye, kidney, lung, nerve, and even death. Diabetes can be diagnosed through blood and urine. In general, glucose detection is carried out using invasive methods that use blood samples, which can cause pain and discomfort for users. Current research is developing non-invasive glucose detection using urine samples. This research aims to develop non-invasive glucose detection technology using 3D-connector μPADs (Microfluidic Paper Based Analytical Devices) which have the advantages of being safe, easy, and simple. The three-dimensional connector on the device functions as a connector to facilitate the coordination of fluid flow in the sample zone and detection zone. The glucose detection method uses gold (III) chloride as a gold nanoparticle (AuNPs) precursor, an aqueous extract of Acalypha indica Linn as a stabilizing agent, sodium hydroxide (NaOH) as a catalyst, and glucose in artificial urine as a sample. Method validation results using imageJ software indicated linearity with a coefficient of determination value (R2) of 0.9714, precision with a %RSD value (Relative Standard Deviation) of 2.69, and an accuracy level ranging from 92.22-99.23%.
Influence of Solvent Polarity on Color Intensity of Marigold Flower Extracts Retnowati, Rurini; Sulistyarti, Hermin; Suratmo; Ulfa, Siti Mariyah; Kamulyan, Budi; Destiara, Irena Amelia; Muzdhalifa, Sefira Putri; Ananda, Regina Ridha
ALKIMIA Vol 10 No 1 (2026): ALKIMIA
Publisher : SCIENCE AND TECHNOLOGY FACULTY OF UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/gfzt5f62

Abstract

In this paper, we report research on the extraction of natural dyes from yellow and orange marigold (Tagetes erecta L.) petals using different solvents. This research aims to determine the optimal dye removal process, investigate stability, and establish color intensity profiles. In order to extract natural dyes that produced various shades of the dried powder of yellow and orange marigold flower petals, two solvents with different polarities, n-hexane and ethyl acetate were used. The maceration procedure using different solvent types was carried out separately. The stability study revealed that all the dyes at room temperature showed better stability with minor changes observed by UV-Visible (UV-Vis) spectrum profiles, indicating that the extracts were stable until the 30th day. To ascertain the color intensity profiles, analytical tests such as UV-Vis spectroscopy and colorimetry utilizing a color reader device were carried out. The color intensity profile of the yellow and orange marigold flower extracts resulting from the UV-Vis spectrum analysis showed that the absorption of the ethyl acetate extract is higher. The pigment concentration, which is also expressed by absorbance, is connected to variations in color intensity. Additionally, the color intensity profile characterization using a color reader revealed that the n-hexane and ethyl acetate extracts had different Commission Internationale d'Eclairage (CIE) L*, a*, and b* values. The yield and color intensity of the extracts were affected by the solvent polarity. In conclusion, the study of the extract's color intensity profile using UV-Vis spectroscopy and a color reader shows that the intensity parameters are aligned.
Sintesis dan Karakterisasi Nanopartikel Fe3O4 termodifikasi Biokompatibel Polimer serta Potensinya sebagai Penghantar Obat Wulandari, Ika Oktavia; Rahayu, Lale B; Riva’i, Imam; Sulistyarti, Hermin; Sabarudin, Akhmad
Indonesian Green Technology Journal Vol. 10 No. 1 (2021): Indonesian Green Technology Journal
Publisher : Sekolah Pascasarjana, Universitas Brawijaya

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Abstract

Nanopartikel sebagai agen penghantar obat berbasis Fe3O4 dalam penelitian ini disintesis dengan dua metode, yakni metode kopresipitasi dan metode sonikasi. Permukaan Fe3O4 selanjutnya dimodifikasi dengan penambahan biokompatibel polimer berupa Polivinil Alkohol (PVA) yang terikat silang dengan Glutaraldehid. Penelitian ini juga dilakukan untuk mengidentifikasi pengaruh komposisi PVA terhadap sifat fisik dan magnetik material yang meliputi ukuran kristal, parameter kisi, dan faktor g-lande. Metode kopresipitasi dengan lama waktu reaksi 30 menit mampu menghasilkan nanopartikel dengan ukuran lebih kecil dibandingkan dengan metode sonikasi dalam durasi waktu reaksi yang sama. Namun demikian, apabila waktu sonikasi diperpanjang yaitu selama 60 menit, maka akan diperoleh nanopartikel bare Fe3O4 dengan ukuran yang lebih kecil. Selanjutnya penambahan PVA dan GA sebagai capping agent pada permukaan Fe3O4 dimaksudkan untuk mereduksi pembentukan agregat dan proses oksidasi dari Fe3O4 untuk menghasilkan fasa besi oksida lainnya. Penambahan polimer PVA meningkatkan ukuran kristal nanopartikel dibandingkan nanopartikel Fe3O4 tanpa PVA. Namun peningkatan ukuran yang terjadi tidak lebih dari 20 nm. Modifikasi permukaan juga mempengaruhi ukuran parameter kisi. Selain itu pelapisan permukaan Fe3O4 dengan PVA dapat mereduksi proses oksidasi nanopartikel Fe3O4 yang dihasilkan. Hal ini terbukti dari intensitas puncak difraktogram yang lebih tinggi dibandingkan bare nanopartikel pada posisi hkl yang sesuai untuk Fe3O4. Penambahan PVA juga mempengaruhi nilai faktor g-lande, dimana faktor ini berkorelasi dengan sifat magnetik dari material. Secara umum penambahan PVA dapat menurunkan nilai faktor g-lande, namun material masih tetap memiliki rekativitas terhadap medan magnet eksternal.
Enzyme-Free Colorimetric Sensing of Glucose using Silver Nanoparticles with a Green Stabilizing Agent from Capsicum chinense Jacq Extract Putra, Boyfannie Ivan; Sulistyarti, Hermin; Utama, Muhammad Mashuri; Haryanto, Aura Ananda; Retnowati, Rurini; Rafi, Mohamad; Sabarudin, Akhmad
Makara Journal of Science Vol. 29, No. 4
Publisher : UI Scholars Hub

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Abstract

Diabetes mellitus (DM) is a significant global health issue characterized by abnormal glucose levels in the human body. DM diagnostics are often based on invasive sampling from blood, which poses a potential risk of infection and tissue damage. Therefore, a non-enzymatic and non-invasive method for glucose monitoring is demanded as a simple alternative to glucose detection in urine samples. In this work, enzyme-free glucose determination is based on the formation of silver nanoparticles stabilized by Capsicum chinense Jacq extract (CCJ-AgNPs). Under alkaline conditions, glucose functions as a reducing agent for silver ions, and the extract acts as a stabilizer to prevent agglomeration. The effect of reaction time, extract concentration, NaOH concentration, and AgNO3 concentration was studied to determine the optimum conditions for glucose sensing (reaction time: 5 min, extract volume: 2 mL, NaOH concentration: 0.05 M, and AgNO3 concentration: 0.2 mM). The linearity of glucose determination was obtained from 0 to 900 mg/L with LOD and LOQ of 1 and 5 mg/L, respectively. The proposed method demonstrated good selectivity against citric acid, NH4Cl, NaCl, H2PO4, and urea at a concentration of 100 mg/L, with good accuracy for the recovery test, ranging from 93.7% to 101.8%. This method can serve as an alternative approach for simple, non-invasive, and non-toxic glucose monitoring in human urine with high sensitivity and specificity.
Non-Enzymatic Detection of Glucose and Ketones in Urine using Paper-Based Analytical Devices 'Aisy, Kamila Rohadatul; Fahmi, Ahmad Luthfi; Sulistyarti, Hermin; Wulandari, Ika Oktavia; Sabarudin, Akhmad
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 9, No 2 (2024): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v9i2.87294

Abstract

Diabetes, driven by unbalanced diets and unhealthy lifestyles, is highly prevalent. In Indonesia, its prevalence is projected to reach 28.6 million by 2045. Microfluidic paper-based analytical devices (μPADs) are paper-based analytical tools that use hydrophilic paper for measurement and hydrophobic barriers to control fluid flow. This research aims to develop a non-enzymatic method for detecting glucose and ketones in artificial urine using S2Z-μPADs. The fabrication of S2Z-μPADs involves printing the design on Whatman No. 1 paper using wax printing and applying silver nanoparticles for glucose detection and the Schiff base reaction for ketone detection. The results show that the optimum condition for glucose detection is achieved with an AgNO3 concentration of 500 mM. A NaOH concentration of 10 M. Acetoacetate detection is optimized with a glycine concentration of 1 M, sodium nitroprusside concentration of 15%, NaOH concentration of 1 M, a drying time of 8 minutes, and a reaction time of 10 minutes. Validation results demonstrate good linearity for glucose (R² = 0.9821) and ketones (R² = 0.995). High precision was achieved with relative standard deviation (RSD) values of 3.792% for glucose and 1.482% for ketones. The obtained limits of detection (LOD) and limits of quantification (LOQ) indicate that the developed S2Z-μPADs can differentiate between each category of diabetes. The accuracy of glucose and ketone detection ranges from 87.463% to 97.374%. The high accuracy of the μPADs highlights their potential for reliable diabetes management and effective disease monitoring.
Co-Authors 'Aisy, Kamila Rohadatul Abdjan, Muhammad Ikhlas Abdul Malik Bahrudin Abner Tonu Lema Adam Wiryawan Afidatul Muadifah Ahmad Luthfi Fahmi Akhmad Sabarudin Akhmad Sabarudin Akhmad Sabarudin Akhmad Sabarudin Akhmad Sabarudin Ananda, Regina Ridha Ani Mulyasuryani Antesar Elmagirbi Aprilia Nur Tasfiyati Asdauna Zahrotun Nisa Asdauna, Asnaili Alfi Nurillah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah Atikah, Atikah, atikah Aulani`am Aulani`am Aulanni`am, Aulanni`am Balqis Milda, Balqis Barlah Rumhayati Bheta Sari D, Bheta Sari Budi Kamulyan Chanif Mahdi Chanif Mahdi Dedeh Kurniasih Dedeh Kurniasih Destiara, Irena Amelia Dewi Annisa’ Dewi Setyowati Dewi Umaningrum Diana Candra Dewi Eka Ratri Noor Wulandari Elvian Eka Krisnaniningrum Erliana Ginting Erwin Sulistyo Erwin Sulistyo Fahmi, Ahmad Luthfi Fahrana Salas Trenggamayu Nelgi Febriyanti, Sita Gunawan, David Haryanto, Aura Ananda Herman Tolle Hikmanita Lisan Nashukha Hilda Emilia Fahriyani, Hilda Emilia Ika Oktavia Wulandari Imam Riva’i Indriana, Silvi Avianti Kamila Rohadatul ‘Aisy Khanifah, Nurul Krisna Rendi Awalludin Kusumaningtyas, Nadia Mira Kusumawardhani, Nury Lale B Rahayu Lilik Suryati Luluil Maknun M. Rafi Mariam Mohamed Omar Alshibani Mega Madha Wijaya Mike Nur Izzati Mochammad Imron Awalludin Mufidah Sari, Puspita Muhammad Iqbal Fahmi Muzdhalifa, Sefira Putri Noerma Juli Azhari Novy Lailatuz Zulfah Nury Kusumawardhani Palupi, Endah Sekar Puspitaloka, Paradhita Ayu Putra, Bhurman Pratama Putra, Boyfannie Ivan Putra, Chairil Anjasmara Robo Putri Dini Putri, Krista Firdaus Suwarno Putri, Lani Artana Qonitah Fardiyah Qonitah Fardiyah Qurrata Ayun Rahayu, Lale B Riva’i, Imam Rizal Nur Huda RR. Ella Evrita Hestiandari Rurini Retnowati Rurini Retnowati Sasangka Prasetyawan Silvi Avianti Indriana Sita Febrianti Siti Mariyah Ulfa Spas D. Kolev Stephanie Lim Suratmo Sutrisno Sutrisno Syarifah, Anisa Lailatusy Tea, Marselina Theresia Djue Tuti Kurniati Tuti Kurniati Utama, Muhammad Mashuri Yudha Ikoma Istanti Yulia Dwi Cahyani, Yulia Dwi Zulfah, Novy Lailatuz Zuri Rismiarti