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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Journal of Food and Pharmaceutical Science Buletin Peternakan VALENSI Jurnal Ilmu Lingkungan AL KAUNIYAH Alchemy : Journal of Chemistry Proceeding International Conference on Global Resource Conservation Elkawnie EduChemia: Jurnal Kimia dan Pendidikan Eksplorium : Buletin Pusat Pengembangan Bahan Galian Nuklir Science and Technology Indonesia Shautut Tarbiyah Al-Kimia Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Ecolab Sulolipu: Media Komunikasi Sivitas Akademika dan Masyarakat Jurnal Riset Sains dan Kimia Terapan al Kimiya : Jurnal Ilmu Kimia dan Terapan Eksplorium : Buletin Pusat Pengembangan Bahan Galian Nuklir
La Ode Sumarlin
Chemistry Study Program, Syarif Hidayatullah State Islamic University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Elkawnie

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Isolation and Cytotoxic Activity of The β-Carotene Combination of Trigona Honey and Namnam Leaves Extract (Cynometra cauliflora) Ahmad Fathoni; La Ode Sumarlin; Fuady Hanief; Dede Sukandar
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 1 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.8696

Abstract

Abstract: Isolation and cytotoxic activity of the β-carotene combination of Trigona honey and Namnam leaves extract (Cynometra cauliflora) were conducted. The urgency of isolating β-carotene compounds because of their known anticancer activity. Namnam leaves are macerated using methanol, then combined with Trigona honey. Fractionation and isolation of β-carotene in combination samples were conducted out by chromatography method. The β-carotene was analyzed using UV-Vis and FTIR spectrophotometer. The cytotoxic activity test was conducted by using the Brine Shrimp Lethality Test (BSLT) method with mortality analysis using Probit Analysis with SPSS v20 and Lethal Concentration 50 (LC50) as parameters. The result showed that the cytotoxic activity of Trigona honey after combination reached 36.6% with an LC50 value of 168.2 ppm, an increase from the Trigona honey sample (LC50 = 265.2 ppm) and Namnam leaves extract (LC50 = 196.12 ppm). The highest cytotoxic activity belongs to the n-hexane fraction (LC50 = 77.6 ppm). The best eluent for β-carotene isolation is petroleum ether. There was increased cytotoxic activity in isolates (LC50 of 22.85 ppm). Isolates were analyzed by UV-Vis and FTIR and compared with standards compound. FTIR analysis results showed that the isolates had functional groups of -CH3 (v 2850-2960 cm-1), -CH3 aliphatic (v 1350-1470 cm-1), C=C (v 2100-2350 cm-1 and 675-870 cm-1), and C-O (v 1000-1300 cm-1). Thus, the combination of Trigona Honey and Namnam leaf extract can be developed as an alternative chemopreventive agent.Abstrak: Isolasi dan aktivitas sitotoksik β-karoten kombinasi madu trigona dan ekstrak daun namnam (Cynometra cauliflora) telah dilakukan. Urgensi mengisolasi senyawa β-karoten karena telah diketahui adanya aktifitas antikanker. Daun namnam dimaserasi menggunakan metanol, untuk kemudian dikombinasikan dengan madu trigona. Fraksinasi dan isolasi β-karoten pada sampel kombinasi dilakukan dengan metode kromatografi. Kandungan senyawa β-karoten dianalisis menggunakan spektrofotometer UV-Vis dan FTIR. Uji aktifitas sitotoksik dilakukan dengan metode Brine Shrimp Lethality Test (BSLT) dengan analisis mortalitas menggunakan Probit Analysis dengan software SPSS v20 dan Lethal Concentration 50 (LC50) sebagai parameter. Hasil analisis menunjukkan bahwa aktivitas sitotoksik madu trigona setelah dikombinasikan mencapai 36,6 % dengan nilai LC50 sebesar 168,2 ppm, meningkat dari sampel madu trigona dengan (LC50 = 265,2 ppm), dan ekstrak daun namnam dengan (LC50 = 196,12 ppm). Aktifitas sitotoksik tertinggi dimiliki oleh fraksi n-heksana(LC50 = 77,6 ppm). Eluen terbaik untuk isolasi β-karoten adalah petroleum eter. Terjadi peningkatan aktifitas sitotoksik pada isolat hasil pemisahan (LC50 sebesar 22,85 ppm). Isolatdianalisis dengan UV-Vis dan FTIR dan dibandingkan dengan senyawa standar. Hasil analisa FTIR menunjukkan isolat memiliki gugus fungsi -CH3 (v 2850-2960 cm-1), -CH3 alifatik (v 1350-1470 cm-1), C=C (v 2100-2350 cm-1 dan 675-870 cm-1), dan C-O (v 1000-1300 cm-1). Dengan demikian, kombinasi Madu Trigona dan ekstrak daun namnam berpotensi untuk dikembangkan sebagai agen kemoprefentif alternatif.
The Total Phenolic, Total Flavonoid, and Brown Pigment in Honey Before and After Heating Achmad Tjachja Nugraha; La Ode Sumarlin; Anna Muawanah; Nurul Amilia; Meyliana Wulandari
Elkawnie: Journal of Islamic Science and Technology Vol 8, No 1 (2022)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v8i1.12757

Abstract

Abstract: Honey has unique components, a characteristic that makes it a valuable food for consumers. It is known that UV radiation and heating impact the quality of honey's bioactive compounds, including total phenolic, total flavonoid, and brown pigment contents. The absorbance of brown pigment at a wavelength of 420 nm, total phenolic at a wavelength of 733 nm, and total flavonoid at a wavelength of 430 nm was measured using a UV-Vis spectrophotometer. The method used in the total phenolic test was the Folin-Ciocalteu method with gallic acid standard (mgGAE/g sample). In contrast, for flavonoids, the method used was the colorimetric method with quercetin standard (mg QE/g sample). The qualitative test results showed that all honey samples contained phenolic and flavonoid compounds. Total phenolic and flavonoid levels showed a decrease in several samples, including TR SLT (Trigona Southeast Sulawesi), TRG BGR (Trigona Bogor), and TR SLS (Trigona Genotrigona Indica South Sulawesi) in the range of 11.8–57.6%. However, most of the total phenolic and flavonoid levels increased after the heating process, i.e., in the samples of AP LMB (apis North Lombok) (25.3% and 88.8%), AP MG (apis mango) (73.1% and 114%), AP MAC (Aceh honey, Buloh Seuma) (8.8% and 199%), TR BIR (Trigona tetroginola biroi South Sulawesi) (58.8% and 146%), and TR LMB (Trigona North Lombok) (44.3% and 84.9%). In contrast, for the formation of brown pigment, there was an increase in all honey samples between 32 and 1.428%. The presence of brown pigment at the end of the heating process of honey samples is thought to have the same role as phenolic compounds and flavonoids, which change the heating process, especially in antioxidant activities and other bioactivities. However, further analysis is needed to prove the conjecture.Abstrak: Madu memiliki komponen yang unik, yang membuatnya menjadi makanan yang berharga bagi konsumen.  Telah diketahui bahwa radiasi UV dan pemanasan berdampak pada kualitas senyawa bioaktif madu, di antaranya kandungan total fenolik, total flavonoid, dan pigmen coklat. Pengukuran absorbansi pigmen coklat pada panjang gelombang 420 nm, pengukuran total fenolik pada panjang gelombang 733 nm dan total flavonoid pada panjang gelombang 430 nm dilakukan menggunakan spektrofotometer UV-Vis. Metode yang digunakan untuk uji total fenolik adalah metode Folin-Ciocalteu dengan standar asam Galat (mgGAE/g sampel), uji flavonoid menggunakan metode Kolorimetri dengan standar kuersetin (mg QE/g sampel).  Hasil uji kualitatif menunjukan bahwa semua sampel madu terdapat senyawa fenolik dan flavonoid. Kadar total fenolik dan flavonoid menunjukkan penurunan pada beberapa sampel, di antaranya TR SLT (Trigona Sulawesi Tenggara, TRG BGR (Trigona Bogor), TR SLS (Trigona Genotrigona Insica Sulawesi Selatan) pada kisaran 11,8 – 57,6%.  Namun, sebagian besar kadar total fenolik dan kadar flavonoid mengalami kenaikan setelah proses pemanasan, yaitu pada sampel AP LMB (apis Lombok Utara) (25,3% dan 88,8%), AP MG (apis mangga) (73,1% dan 114%), AP MAC (madu Aceh, Buloh Seuma) (8,8% dan 199%), TR BIR (Trigona Tetroginola biroi sulawesi selatan) (58,8% dan 146%), TR LMB (Trigona North Lombok) (44,3% dan 84,9), sedangkan untuk pembentukan pigmen coklat, terjadi peningkatan pada semua sampel madu di antara 32 – 1.428%. Keberadaan pigmen coklat pada akhir proses pemanasan, diduga memiliki peran yang sama dengan senyawa fenolik dan flavonoid yang mengalami perubahan selama proses pemanasan, terutama pada aktivitas antioksidan dan bioaktivitas lainnya.  Namun diperlukan analisis lebih lanjut untuk membuktikan dugaan tersebut.
Co-Authors . Faturrahman . Masitoh . Suryatna A.S. Satyaningtijas Achmad Tjachja Achmad Tjachja Nugraha Achmad Tjahja Ade Candra Agik Suprayogi Ahmad Fathoni Ahmad Fathoni Ahmad Saepul Mujab Ahmad Tjahja Nugraha, Ahmad Tjahja Alfida Zakiyah, Alfida Alfionita Octa Nur Zidni Andhi Vitaria Andriantoro Andriantoro Anna Muawanah Arif Sumantri Arif Sumantri Bagus Sediadi Bandol Utomo Barokah Aliyanta Dany Poltak Marisi Dede Sukandar Dikdik Mulyadi Dina Fransiska Eka Rizki Amelia Eka Rizki Amelia Ericka Putri Wijaya Farhan Riza Afandi Farida Ariyanti Fuady Hanief Hajar Hajar Hajar Hajar Hendrawati Hendrawati Hilyatuz Zahroh Hilyatuz Zahroh Ihya Sulthonuddin Iin Ana Rizqi Ika Amalia, Ika Jane Ratini Putri Lela Mukmilah Yuningsih Lia Pratiwi M. Iqbal Suryatama Manadzhir Mahalli Marisi, Dany Poltak Megga Ratnasari Pikoli Melina Hadera Meyliana Wulandari Nadya Uswatun Hasanah Nani Radiastuti Nida Sopiah Nur Enita Nur Ernita Nur Ernita Nur Ernita Nurhasni, N. Nurul Amilia Nurul Amilia Prima Soheti Prita Wardhani Rahminiwati, Min Ratna Istianah Riana Octavia Rita Mukhtar S Hermanto Salih Muharam Sandra Hermanto Siti Nurbaya Siti Nurbayti Soheti, Prima Sri Yadial Chalid Sri Yadial Chalid Suharyono Suharyono Syed Azhar Syed Sulaiman, Syed Azhar Syed Syifa Fauziah Teguh Wahyono Tri Retno Dyah Larasati Widya Fatimah Yoga Asmara Yunesfi Syofyan Zakki R Mubarok