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All Journal Medical Journal of Indonesia Majalah Kedokteran The Indonesian Biomedical Journal Paediatrica Indonesiana Jurnal Entomologi Indonesia ASEAN Journal of Community Engagement eJournal Kedokteran Indonesia Archives of Pediatric Gastroenterology, Hepatology, and Nutrition The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
Saleha Sungkar
Department Of Parasitology Faculty Of Medicine Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Journal : the indonesian biomedical journal

 1 
Hyaluronic Acid Accelerates VEGF and PDGF Release from Advance Platelet Rich Fibrin in Diabetic Foot Ulcer Ronald Winardi Kartika; Idrus Alwi; Franciscus Dhyanagiri Suyatna; Ferry Sandra; Em Yunir; Sarwono Waspadji; Suzzana Immanuel; Todung Silalahi; Saleha Sungkar; Jusuf Rachmat; Saptawati Bardosono; Mirta Hediyati Reksodiputro
The Indonesian Biomedical Journal Vol 13, No 3 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i3.1523

Abstract

BACKGROUND: Hyaluronic acid (HA) is an essential component of extracellular matrix and mediates signaling in wound healing. HA could induce growth factor release from Advanced Platelet Rich Fibrin (A-PRF), including Vascular Endothelial Growth Factor (VEGF) and Platelet-derived Growth Factor (PDGF). However, concentrations of the released-VEGF and PDGF have not been clearly disclosed. Therefore, current study was conducted to measure the release of these growth factors in HA + A-PRF gel of diabetic foot ulcer (DFU) subjects.METHODS: Twenty DFU subjects were included in the study and treated with A-PRF or HA+A-PRF. A-PRF was derived from autologous peripheral blood and processed with low-speed centrifugation. HA was added with a ratio of 1:0.6. A-PRF or HA + A-PRF was applied topically on DFU. Upper tips of A-PRF or HA + A-PRF gels were collected on day 0, 3 and 7 for measurements of VEGF and PDGF concentrations with Enzyme-linked Immune-sorbent Assay (ELISA) methods.RESULTS: On day-3, both VEGF and PDGF concentrations of HA + A-PRF group were significantly higher than the VEGF (p=0.000) and PDGF (p=0.019) concentrations of A-PRF group. The VEGF and PDGF concentrations were continuously and significantly increased on day-7 of HA + A-PRF group, compared to the VEGF (p=0.000) and PDGF (p=0.004) concentrations of A-PRF group.CONCLUSION: Combination HA+A-PRF induces VEGF and PDGF release from A-PRF. A mixture of A-PRF and HA could be more effective than A-PRF alone for treatment of DFU.KEYWORDS: hyaluronic acid, advanced platelet rich fibrin, PRF, growth factor, VEGF, PDGF, diabetic foot ulcer
Advance-Platelet Rich Fibrin and Hyaluronic Acid Combination Improves Interleukin-6 and Granulation Index in Diabetic Foot Ulcer Patients Ronald Winardi Kartika; Idrus Alwi; Franciscus Dhyanagiri Suyatna; Em Yunir; Sarwono Waspadji; Suzanna Immanuel; Todung Silalahi; Saleha Sungkar; Jusuf Rachmat; Saptawati Bardosono; Mirta Hediyati Reksodiputro
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1501

Abstract

BACKGROUND: Diabetic foot ulcer (DFU) is the most common and threatening complication of Diabetes Mellitus (DM). Ideal wound dressing for DFU management should relieve symptoms, provide wound protection, and encourage healing. Advanced-Platelet Rich Fibrin (A-PRF) and Hyaluronic Acid (HA) have been proven to improve wound healing process. This study was aimed to demonstrate the ability of combination of A-PRF and HA in reducing inflammation and improving DFU tissue healing.METHODS: Twenty DFU subjects were involved in this study, and divided into two groups based on the topical fibrin gel treatment: A-PRF + HA group and A-PRF only group. A-PRF was obtained by peripheral blood centrifugation. A-PRF + HA was prepared by homogening A-PRF and AH with a ratio of 1:0.6. Interleukin-6 (IL-6) level, granulation index (GI), numeric pain score (NPS), and inflammation clinical symptoms (ICS) were assessed on day-0, 3, 7 and 14.RESULTS: Wound swabs’ IL-6 level on day-7 was found to be significantly lower in A-PRF + HA compared to A-PRF alone (p=0.041). The IL-6 level reduction also found to be significant higher either in wound swabs (day 0-7, p=0.015) or fibrin gel (day 0-3, p=0.049; day 0-7, p=0.034). A-PRF + HA treatment significantly increased the GI even since day-3 (p=0.043), with lower NPS (p<0.001), and ICS score.CONCLUSION: The combination of A-PRF and HA increases the GI in DFU healing by reducing the inflammation state which will induce the angiogenesis process, as well as reducing pain in DFU subjects better than A-PRF alone.KEYWORDS: inflammation, interleukin-6, wound healing, angiogenesis, proliferation 
Co-Authors Agnes Kurniawan Agus Aulung Ahmad Fuady Ahmad P. Natanegara Aji W. Wardhana Amajida Fadia Ratnasari Anastasia Yoveline Anastasia Yoveline Andi Arus Victor Anthea Casey Aria Kekalih Ayudianingrum, Asti Badriul Hegar Budiman Prakoso Cara Tasya Handita Dani M. Trianto Danny Surya Dewita Kamaruddin Diadikma Bellarosa Em Yunir, Em Evita Karianni Bermanshah Fanny P. Irmawati Ferry Sandra Franciscus D. Suyatna Ghina Fedora Gladys - Gladys Kusumowidagdo Gozali, Meutia N. Guntur Darmawan Hansen Angkasa Hasan Mihardja Hasan Mihardja Herqutanto - Hilman Zulkifli Amin Hirzi Zulkarnain Hisar Daniel I Gusti N.A.A. Oktafandi Idrus Alwi Idrus Alwi Indra  Sianturi Irma Annisa Isfiyanto, Isfiyanto Julie Barliana Julie Dewi Barliana Jusuf Rachmat Jusuf Rachmat Kartika Hajarani Keiko Gunardi Kusumowidagdo, Gladys Laura Agnestasia Djunaedi Luddwi A. Rizky Matiur, Eiko B. Mohamad A. Sahala Monica E.T. Rosandi Muhamad S. Advani Muhammad P. Wiratama Murdani Abdullah Murdani Abdullah Nadjib Advani Pratiwi Sudarmono R. Sapto Hendri Boedi Soesatyo Ratna Sitompul Ratna Sitompul Ratna Sitompul Rawina Winita Rawina Winita Reksodiputro, Mirta Hediyati Reza Damayanti Rikeish R. Muralitharan Rilanda, Risa Rinawati Rohsiswatmo Rita Sitorus Ronald Winardi Kartika Rose A. Haswinzky Rossalyn Andrisa Sandra Widaty Santoso, Dewi Irawati Soeria Saptawati Bardosono Saptawati Bardosono Sari, Della P. Sarwono Waspadji Sarwono Waspadji Soefiannagoya Soedarman Soleman, Imam Rahadian Sri L. Menaldi Sri Wahdini Sri Wahdini Sri Wahdini Sukartini, Ninik Suzanna Immanuel Suzzana Immanuel Tambunan, Fitry B.J. Tanjungsari, Dian Wahyu Tetty Yuniarti Todung Silalahi Todung Silalahi Tri Rahayu Triana Agustin Victor Santawi Wirastuti, Aulia Wresti Indriatmi Yasmine A. Dwinastiti Yeni D. Lestari, Yeni D. Yeni Dwi Lestari Yudo Irawan Yusuf Ananda Yvan Vandenplas Zahrotul Habibah