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NATURAL PRODUCTS PHARMACEUTICAL STANDARDIZATION TOWARDS PHYTOPHARMACA FOR INDONESIAN TRADITIONAL MEDICINE DEVELOPMENT: STANDARDISASI FARMASITIKAL BAHAN ALAM MENUJU FITOFARMAKA UNTUK PENGEMBANGAN OBAT TRADISIONAL INDONESIA Yuslianti, Euis Reni; Bachtiar, Boy M; Suniarti, Dewi Fatma; Sutjiatmo, Afifah B
Dentika: Dental Journal Vol. 19 No. 2 (2016): Dentika Dental Journal
Publisher : TALENTA

There is a tendency back to nature treatment in Indonesian community because modern/synthetic medicines is expensive and have a various alarming side effects. That is why natural products medicine becomes more popular, aside from mass media promotions. Researches on natural products such as herbal medicine increases along with the desire to get phytofarmaca that can compete with modern medicines. The aims of this literature review are to discuss and provides information on pharmaceutical standardization of natural products toward phytopharmaca for developing Indonesian traditional medicine. Pharmaceutical standardization will strengthen the basic of pharmaceutical science in producing safe, efficacy, and qualify traditional medicine products. The pharmaceutical standardization consists of standardizing simplicial quality, safety, efficacy, and stable formulation of medicines for use in health services.

EFFECT OF CHITOSAN ON PROTEIN CONTENT IN THE MEDIUM CULTURE OF OSTEOBLASTS EXPOSED TO OXIDATIVE STRESS: EFEK KITOSAN TERHADAP KANDUNGAN PROTEIN DALAM KULTUR MEDIUM OSTEOBLAS YANG DIPAPAR DENGAN STRES OKSIDATIF Shanty Chairani; Sri Utami; Dewi Fatma Suniarti
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Chitosan is a derivative of chitin which has potential for use in bone regeneration and has been reported can stimulate bone formation. Oxidative stress as one cause of bone damage, was found increased in osteoporosis, periodontitis and arthritis. One of the species oxygen reactive (ROS), hydrogen peroxide, has been reported can inhibit osteoblast proliferation. This study was aimed to investigate the effect of various chitosan concentrations on protein content in the culture medium of human osteoblast-like cell line, MG 63, which was exposed to hydrogen peroxide. MG 63 cells were exposed to various chitosan concentrations (% w/v) 0.1, 0.2, 0.4, and 1.6%. Culture cells without chitosan were used as a control. Cells were growth with Î±-MEM medium (37ÂºC, 5% CO2) until they became confluent, then they were exposed to hydrogen peroxide for 4 hours. The protein content in the culture medium was measured by using Bradford protein assay at 655 nm wavelength. The result showed that hydrogen peroxide decreased protein concentration in the medium culture compared with group without hydrogen peroxide. Treatment group with chitosan concentration 0.4% and 1.6% exhibited a significant increasing of protein concentration in osteoblast culture medium compared with control. In conclusion, in osteoblast culture medium chitosan can inhibit the decreasing of total protein concentration which was caused by oxidative stress.

Perbedaan kadar enzim α-amylase saliva pada penerbang sipil Indonesia yang mengalami stress dan tidak stress karena faktor kelelahanDifferences in the salivary α-amylase levels in Indonesian civil aviators with and without fatigues stress experience Meta Yunia Candra; Dewi Fatma Suniarti; Febriana Setiawati; Nurtami Soedarsono
Padjadjaran Journal of Dental Researchers and Students Vol 4, No 2 (2020): Oktober 2020
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v4i2.28063

Pendahuluan: Penerbang Sipil Indonesia beresiko mengalami stres yang disebabkan karena faktor kelelahan dengan rute penerbangan jarak dekat antara pulau-pulau di Indonesia. Enzim α-amylase saliva dapat digunakan sebagai biomarker stress. Penelitian ini bertujuan untuk menganalisis perbedaan kadar enzim α-amylase saliva pada penerbang sipil Indonesia yang mengalami stres karena faktor kelelahan dengan penerbang sipil Indonesia yang tidak mengalami stress karena faktor kelelahan. Metode: Jenis penelitian adalah cross-sectional, membandingkan tingkat kelelahan yang menjadi penyebab stress pada penerbang sipil Indonesia dengan metode pengukuran Fatigue Severity Scale (FSS) dan membandingkan kadar enzim α-amylase saliva yang dianalisa dengan metode ELISA pada dua kelompok penerbang sipil Indonesia yang memiliki jam terbang total lebih dari 6624 jam dan melakukan penerbangan sektor sebanyak 24 subjek dengan kelompok penerbang sipil Indonesia yang memiliki jam terbang total kurang dari 6624 jam dan tidak melakukan penerbangan sektor sebanyak 16 subjek dengan uji analisis data bivariat komparatif. Hasil: Kelompok penerbang sipil Indonesia dengan jam terbang total lebih dari 6624 jam dan melakukan penerbangan sektor menunjukkan nilai enzim α-amylase sebesar 0,899 dan kelompok penerbangan sipil Indonesia dengan jam terbang kurang dari 6624 jam dan tidak melakukan penerbangan sektor menunjukkan nilai enzim α-amylase sebesar 0,689. Terdapat perbedaan bermakna p = 0,006 (p<0,05) antara kedua kelompok penerbang. Simpulan : Stres yang disebabkan karena faktor kelelahan pada penerbang sipil Indonesia menghasilkan kadar enzim α-amylase yang lebih tinggi dibandingkan dengan penerbang sipil Indonesia yang tidak mengalami stress karena faktor kelelahan.Kata kunci: Enzim α-amylase saliva, fatigue severity scale, stres, penerbang. ABSTRACT Introduction: Indonesian civil aviators have a risk of experiencing stress due to fatigue factor with high-frequency intersection routes between islands of Indonesia. Salivary α-amylase is an enzyme that can be used as a stress biomarker. This study was aimed to analyse the differences of salivary α-amylase levels in Indonesian civil aviators with and without fatigues stress experience. Methods: This research was cross-sectional, comparing the level of fatigue as the cause of stress for Indonesian civil aviators with the Fatigue Severity Scale (FSS) measurement method. The level of the salivary α-amylase enzyme was analysed using the ELISA method in two groups: Indonesian civil aviators with more than 6624 flight hours and performed sector flights (24 subjects); Indonesian civil aviators with less than 6624 flight hours and did not perform sector flights (16 subjects). All data then analysed with comparative bivariate data analysis. Results: The group of Indonesian civil aviators with more than 6624 flight hours and performed sector flight showed an α-amylase enzyme value of 0.899; while the group of Indonesian civil aviators with less than 6624 flight hours and did not perform sector flights showed an α-amylase enzyme value of 0.689. There was a significant difference p=0.006 (p < 0.05) between the two groups of pilots. Conclusion: Indonesian civil aviators with fatigues stress experience of increases the α-amylase enzyme value compared to Indonesian civil pilots without fatigues stress experience. Keywords: Salivary α-amylase, fatigue severity scale, stress, aviator.

RAMBUTAN HONEY PHARMACEUTICAL STANDARDIZATION FOR WOUND HEALING TREATMENT Euis Reni Yuslianti; Boy Muclish Bachtiar; Dewi Fatma Suniarti; Afifah Bambang Sutjiatmo
Bionatura Vol 17, No 1 (2018): Bionatura Maret 2018
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Human wounds require proper management so they may heal faster and without complication. Honey is empirically effective to accelerate wound healing but scientifically has been proven yet as a pharmaceutical product is very limited. This study aimed to obtain rambutan honey pharmaceutical grade by setting specific and nonspecific parameters of simplicia. The research method was experiments laboratory by nonspecific parameters test (diastase enzyme activity, HMF, pH value, moisture content, ash content, glucose level, heavy metal contamination: Pb, Cd, Hg, and As, chloramphenicol, microbial contamination: coliform, and yeasts) as well as specific parameters (simplicia identity and organoleptic test). The results showed that rambutan honey proved had good quality of specific and nonspecific parameters. Rambutan honey water content has been pharmaceutical standardized according to drug standard that is to be 10.15%. Based on the results of this study it is necessary to test rambutan honey efficacy in vivo and in vitro and effects of rambutan honey topical products to humans towards phytopharmaca medicine.

An experimental model of chemically-induced ulceration of the buccal mucosa of Mus musculus Idrus, Erik; Hartanti, Pratiwi Dwi; Suniarti, Dewi Fatma; Prasetyo, Sri Redjeki; Wimardhani, Yuniardini Septorini; Subarnbhesaj, Ajiravudh
Makara Journal of Health Research Vol. 23, No. 3
Publisher : UI Scholars Hub

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Background: Ulceration caused by chemical agents used in dental practice for in-office or home-used is a common event, resulting in discomfort and pain. Treatments for such conditions are still being developed, requiring extensive experiments both in vitro and in vivo studies. At present, a standardized experimental mouse model for mucosal ulceration caused by a chemical inducer to study the pathogenesis of ulceration and to develop medications for treatment of ulceration is still not available. The aim of this study was to create a chemically induced model of ulceration of the buccal mucosa of mice. Methods: An in vivo study model of ulceration using a total of 9 mice (Swiss Webster) was performed. All mice received 70% acetic acid application on the left buccal mucosa, while the right buccal mucosa received only saline. Clinical and histological observations of ulcer formation and healing were performed, including the presence of redness and swelling, ulcer diameter, bodyweight as well as epithelial disintegration, dilation of blood vessels, and infiltration of inflammatory cells. Results: Buccal mucosa application of 70% acetic acid generated ulcers on day 2, reached its peak on day 3 and recovered by day 14. The histological features of inflammation were also seen in the ulcer model, and the degree of inflammation was consistent with the day of ulcers. Conclusion: Chemical trauma by the administration of 70% acetic acid effectively induce ulceration on buccal mucosa in mice, and this method can be considered as a novel, reproducible, and clinically relevant model to study pathogenesis and therapeutic approach for treating oral mucosal ulceration.

Vitamin administration on orthodontic tooth movement animal model: A systematic review Lubis, Hilda Fitria; Idrus, Erik; Suniarti, Dewi Fatma; Amir, Lisa Rinanda; Bachtiar, Endang Winiati; Lestari, Widya
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 2 (2025): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i2.p207-218

Background: Duration is a very important aspect of orthodontic treatment and is still challenging for orthodontists. Numerous studies investigating the effects of biological substances, including dietary supplements, on orthodontic tooth movement (OTM) rate indicate positive results. Efforts to improve the OTM rate can be classified into four main categories: biological, biomechanical, physical, and surgical. Numerous animal studies have evaluated the impact of biological substances on the rate of OTM, yielding positive outcomes compared to those not given biological substances. Purpose: This systematic review investigated the impact of dietary supplement delivery both locally and systemically on the rate of OTM. Methods: Nine databases were searched until January 31, 2023, for animal studies evaluating the effect of supplement administration on OTM. The Systematic Review Center for Laboratory Animal Experimentation’s (SYRCLE) risk of bias tools were employed. This review’s reporting adhered to the PRISMA guidelines. Results: Sixteen studies were identified for inclusion. Local injections of vitamin D exhibited variable effects. Vitamin C and zinc, as well as vitamin A, showed insignificant effects based on the OTM rate. Vitamin E showed conflicting results. Combined prostaglandin E2 (PGE2) and calcium can increase the OTM. Effects of systemic administrations of omega-3 fatty acids can decrease the OTM in vivo. Conclusion: The pace of tooth movement in animals may vary depending on the local or systemic administration of vitamins, as applied to OTM animal models.

The role of fibroblast growth factor receptor-3 as a negative regulator in late mandibular growth: Peran fibroblast growth factor receptor-3 sebagai regulator negatif di akhir pertumbuhan mandibula Pratiwi, Dwita; Suniarti, Dewi Fatma; Soedarsono, Nurtami; Soegiharto, Benny M.
Makassar Dental Journal Vol. 14 No. 1 (2025): Volume 14 Issue 1 April 2025
Publisher : Makassar Dental Journal PDGI Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35856/mdj.v14i1.1207

Assessment of mandibular growth status has been done using cephalometric radiographs by looking at cervical bone maturation. Recent studies suggest that this method is not good at identifying the final period of growth. In recent years, biomolecular re-search has been conducted to look at the potential of proteins as growth indicators. It is important to comprehensively understand the mandibular growth process in order to determine which proteins have the potential to be used as indicators. Mandibular growth is a complex biological process that is regulated by various proteins through various signalling pathways. The centre of growth is at the mandibular condyle which has a unique cartilage and plays a dual role as articular cartilage and growth plate. This review discusses the role of fibroblast growth factor receptor-3 (FGFR-3) in the mandibular growth process and its signalling pathways. The FGFR-3 was shown to act as a negative regulator of mandibular growth by reducing the proliferation and hypertrophic dif-ferentiation of chondrocytes in mandibular condylar cartilage. It is concluded that the regulation of FGFR-3 is localised due to pre-vious studies on condylar cartilage of experimental animals, so further research needs to be done to see the role of FGFR-3 in human mandibular growth.

The role of Treponema denticola in the periodontitis alveolar bone damage: Systematic review Idrus, Erik; Hartanto, Willy; Lestari, Widya; Suniarti, Dewi Fatma
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.314-325

Background: Periodontitis is a chronic inflammation condition of the periodontal tissue, which causes irreversible, progressive deterioration of the tooth-supporting tissue, leading to tooth loss. One of the main bacteria in the pathogenesis of periodontitis is Treponema denticola. Its involvement in periodontitis includes producing and releasing various virulence factors that further modulate inflammation and ignite alveolar bone destruction. Objective: To examine Treponema denticola's role in the mechanism of alveolar bone damage in periodontitis. Method: This systematic review was conducted from August 2021 until April 2022. Qualified literature is evaluated based on inclusion criteria, including published in English within the last ten years and research articles available in full text. The inclusion literatureâ€™s determination was based on the PRISMA (Preferred Reporting Item for Systematic Review and Meta-Analysis). Result: Six journals fit the inclusion criteria and discuss Treponema denticola involvement in alveolar bone destruction via several mechanisms, including periplasmic flagella as its virulence factor. Treponema denticola can inhibit osteogenic cell differentiation and induce the production TNF-Î±, IL-6, and IL-1Î², which are the proinflammatory cytokines involved in osteoclastogenesis. The induction mechanism of these various cytokines can ultimately increase osteoclast differentiation by increasing RANKL expression and decreasing OPG expression. Conclusion: Treponema denticola is involved in alveolar bone destruction by inhibiting bone formation and inducing an inflammatory response in immune cells that can increase osteoclast differentiation, as observed in alveolar bone destruction.

The role of Treponema denticola in the periodontitis alveolar bone damage: Systematic review Idrus, Erik; Hartanto, Willy; Lestari, Widya; Suniarti, Dewi Fatma
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.314-325

Background: Periodontitis is a chronic inflammation condition of the periodontal tissue, which causes irreversible, progressive deterioration of the tooth-supporting tissue, leading to tooth loss. One of the main bacteria in the pathogenesis of periodontitis is Treponema denticola. Its involvement in periodontitis includes producing and releasing various virulence factors that further modulate inflammation and ignite alveolar bone destruction. Objective: To examine Treponema denticola's role in the mechanism of alveolar bone damage in periodontitis. Method: This systematic review was conducted from August 2021 until April 2022. Qualified literature is evaluated based on inclusion criteria, including published in English within the last ten years and research articles available in full text. The inclusion literatureâ€™s determination was based on the PRISMA (Preferred Reporting Item for Systematic Review and Meta-Analysis). Result: Six journals fit the inclusion criteria and discuss Treponema denticola involvement in alveolar bone destruction via several mechanisms, including periplasmic flagella as its virulence factor. Treponema denticola can inhibit osteogenic cell differentiation and induce the production TNF-Î±, IL-6, and IL-1Î², which are the proinflammatory cytokines involved in osteoclastogenesis. The induction mechanism of these various cytokines can ultimately increase osteoclast differentiation by increasing RANKL expression and decreasing OPG expression. Conclusion: Treponema denticola is involved in alveolar bone destruction by inhibiting bone formation and inducing an inflammatory response in immune cells that can increase osteoclast differentiation, as observed in alveolar bone destruction.

The Number of Alveolar Bone Cells Exposed to Zoledronate in the Post-Tooth Extraction Socket Lubis, Septina Sabilla; Palupi, Anggraeny Putri Sekar; Komariah, Komariah; Latief, Benny Syariefsjah; Priosoeryanto, Bambang Pontjo; Suniarti, Dewi Fatma
Journal of Syiah Kuala Dentistry Society Vol 9, No 2 (2024): December 2024
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jds.v9i2.41502

Background: Zoledronate is an intravenous bisphosphonate that effectively treats osteoporosis by inhibiting osteoclast resorption, thereby reducing the risk of fractures. However, its use in post-extraction patients may increase the risk of bisphosphonate-related osteonecrosis of the jaws (BRONJ). Objective: This study aims to analyze the effects of zoledronate on alveolar bone structure in Sprague Dawley rats, focusing on changes in osteoblast, osteocyte, and osteoclast counts. Methods: This cross-sectional study employed an observational analytical approach. Samples were categorized based on zoledronate exposure. Osteoblast, osteocyte, and osteoclast counts were measured using ImageJ software at 40x magnification. Data were analyzed using SPSS, with one-way ANOVA employed for statistical analysis. Normality and homogeneity tests were conducted to ensure data validity. Results: Zoledronate exposure resulted in a decrease in osteoblast and osteoclast counts, while osteocyte counts increased. Statistical analysis showed no significant differences between the zoledronate-exposed group and the control group. Conclusion: Zoledronate reduces osteoclasts and increases osteocytes, potentially leading to cellular apoptosis in osteoporosis patients. The risk of BRONJ can be minimized by administering zoledronate at appropriate doses and maintaining optimal oral hygiene to prevent bacterial activity.

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