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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Pendidikan Indonesia AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Naturalistic : Jurnal Kajian dan Penelitian Pendidikan dan Pembelajaran Syntax Literate: Jurnal Ilmiah Indonesia JURNAL PENDIDIKAN TAMBUSAI Jurnal Basicedu Jurnal Review Pendidikan dan Pengajaran (JRPP) JURNAL TEKNIK HIDRAULIK JURNAL SUMBER DAYA AIR Jurnal Agrotropika Jurnal Ilmiah Dozen Globalindo Sibatik Journal : Jurnal Ilmiah Bidang Sosial, Ekonomi, Budaya, Teknologi, Dan Pendidikan ADINDAMAS: Jurnal Pengabdian Kepada Masyarakat Makara Journal of Science Jurnal Basicedu JURNAL PARADIGMA: Journal of Sociology Research and Education Innovative: Journal Of Social Science Research Transforma: Jurnal Manajemen Juni-Jurnal Pelita Nusa Harmony : Jurnal Pembelajaran IPS dan PKN
Yadi Suryadi
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. (0251) 8337975
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Journal : AL KAUNIYAH

 1 
PATOGENISITAS Beauveria bassiana STRAIN STGD 7(14)2 DAN STGD 5(14)2 TERHADAP WERENG COKLAT (Nilaparvata lugens STÃ…L) Suryadi, Yadi; Wartono, Wartono; Susilowati, Dwi Ningsih; Lestari, Puji; Nirmalasari, Cyntia; Suryani, Suryani
Al-Kauniyah: Jurnal Biologi Vol 11, No 2 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (910.742 KB) | DOI: 10.15408/kauniyah.v11i2.6694

Abstract

AbstrakBeauveria spp. merupakan salah satu jamur entomopatogen yang dapat digunakan sebagai agen biokontrol terhadap berbagai serangga hama. Tujuan penelitian ini adalah untuk menguji patogenitas Beauveria spp. terhadap serangga wereng coklat (Nilaparvata lugens Stål). Isolat-isolat Beauveria spp. diisolasi dari serangga walang sangit dari Situ Gede, Bogor, Jawa Barat. Identifikasi jamur dilakukan berdasarkan analisis data sekuen dari daerah Internal Transcribed Spacer (ITS) rDNA. Hasil uji menunjukkan bahwa patogenisitas isolat Stgd 5(14)2 dan Stgd 7(14)2 menghasilkan tingkat mortalitas 100% terhadap N. lugens. Isolat Stgd 5(14)2 dan Stgd 7(14)2 memiliki nilai LT50 yang rendah, danmenyebabkan kematian yang cepat terhadap wereng coklat. Hasil analisis filogenetik menunjukkan bahwa sekuen Stgd 5(14)2 dan Stgd 7(14)2 termasuk ke dalam spesies Beauveria bassiana s.str. Hasil studi ini merupakan kajian awal terhadap patogenisitas jamur entomopatogen B. bassiana terhadap wereng coklat, dan distribusinya di pertanaman padi Situ Gede, Jawa Barat.Abstract Beauveria spp. is one of the entomopathogenic fungi that can be used as biocontrol agents against various insect pests, including brown planthopper (Nilaparvata lugens Stal). This study aimed to test the pathogenicity of Beauveria spp. against N. lugens. Beauveria spp. were isolated from the rice stink bug insects, collected from Situ Gede, Bogor, West Java. Fungal identification was carried out based on the internal transcribed spacer (ITS) rDNA analysis. The pathogenicity assay revealed that Stgd 5(14)1 and Stgd 7(14)2 isolates were virulent against N. lugens, with a mortality of 100%. The LT50 (median lethal time) determination, indicated that Stgd 5(14)2  and Stgd 7(14)2 isolates had low value indicating a faster mortality in brown planthopper insects. Phylogenetic analysis showed that the sequences of Stgd 5(14)2 and Stgd 7(14)2 belong to Beauveria bassiana s.str. This is the preliminary pathogenicity trial of entomopathogenic fungi B. bassiana against brown planthopper and their distribution in rice-growing, Situ Gede area in West Java.
Isolation And Characterization of Bacteria from Shallots (Allium cepa L.) as In-vitro Biocontrol Agent of Fusarium oxysporum f.sp cepae Radiastuti, Nani; Amalia, Wuliani; Suryadi, Yadi; Susilowati, Dwi N; Kosasih, Jajang
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.38132

Abstract

AbstractShallot is one of the leading vegetable commodities with many benefits such as for seasonings and herbal medicinal ingredients. The demand for shallots continues to increase; however, shallot production is still relatively low. One of the limiting factors causing low shallot production is due to wilt disease caused by Fusarium oxysporum f.sp cepae (Foc). Bacteria have many roles in suppressing the growth of Foc, and this study aims to obtain potential bacterial isolates from the shallot plant to inhibit the growth of Foc Based on fungal diameter zone inhibition, degree of inhibition, and chitinase test, it was obtained 9 isolates which could suppress the growth of Foc. The results indicated that the AB3, TB2, and UB1 bacterial isolates could inhibit the growth of Foc with a percentage of inhibition of 46.80; 40.24; and 35.11%, respectively. The analysis showed that AB3, TB2, and UB1 isolates were categorized as moderate in suppressing the growth of Foc. The 16S rRNA sequencing results showed that AB3 and TB2 isolate had similarities with Bacillus subtilis by 99,75%, and 100%, respectively, while UB1 isolate had similarities with Pseudomonas nitroreducens by 89,35%. Based on the result showed that Bacillus sp. AB3 and TB2 isolates, and P. nitroreducens UB1 isolate have more potential as biological control agents to control the Fusarium wilt at in vitro assay. The field efficacy studies on these potential antagonists need to be done in the future.AbstrakSalah satu faktor pembatas yang menyebabkan rendahnya produksi bawang merah adalah penyakit layu yang disebabkan oleh Fusarium oxysporum f.sp cepae (Foc). Bakteri antagonis memiliki banyak peran dalam menekan pertumbuhan Foc, dan penelitian ini bertujuan untuk mendapatkan isolat bakteri antagonis potensial asal tanaman bawang merah untuk menghambat pertumbuhan Foc. Berdasarkan nilai zona hambat diameter jamur, derajat hambat dan uji kitinase, diperoleh 9 isolat yang dapat menekan pertumbuhan Foc. Hasil penelitian menunjukkan bahwa isolat bakteri AB3, TB2, dan UB1 mampu menghambat pertumbuhan Foc dengan persentase penghambatan masing-masing sebesar 46,80; 40,24; dan 35,11% dengan kategori penghambatan pertumbuhan Foc moderat. Hasil sekuensing 16S rRNA, menunjukkan bahwa isolat AB3 dan TB2 memiliki kemiripan dengan Bacillus subtilis masing-masing sebesar 99,75%, dan 100%, sedangkan isolat UB1 memiliki kemiripan dengan Pseudomonas nitroreducens sebesar 89,35%. Berdasarkan hasil penelitian menunjukkan bahwa Bacillus sp. isolat AB3, TB2, dan P.nitroreducens isolat UB1 berpotensi digunakan sebagai agen pengendali hayati untuk mengendalikan penyakit layu Fusarium pada uji in vitro. Studi kemanjuran lapangan terhadap isolat antagonis potensial ini perlu dilakukan di masa depan.
In Vivo Testing of Pseudozyma hubeiensis to Control Aspergillus flavus in Nutmeg Seeds (Myristica fragrans Houtt.) Rahayuningsih, Sri; Hidayah, Mudrikah Nurul; Susilowati, Dwi Ningsih; Supriadi; Suryadi, Yadi; Rismayani; Radiastuti, Nani
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.46462

Abstract

Nutmeg (Myristica fragrans Houtt.) is a high-value spice commodity from Indonesia, often contamination with aflatoxin-producing Aspergillus flavus. Previous research has identified two epiphytic yeast isolates from nutmeg plants, namely DP1341a and DP1342, both of which were identified as Pseudozyma hubeiensis and showed antagonistic potential against A. flavus. This study is a follow- up study that aims to test the antagonistic activity of both isolates against A. flavus in vivo on nutmeg seeds. The research was conducted experimentally through several stages, namely testing the compatibility of isolates on Yeast Malt Agar (YMA) media, determining the optimum fermentation time, and applying liquid culture and fermentation filtrate to 120 nutmeg seeds. Results showed that the consortium of isolates DP1341a and DP1342 did not increase the inhibitory power results compared to single cultures with optimum fermentation times of 10 and 14 days, respectively. In vivo applications show that treatment with DP1342 isolate (KC-DP1342) and consortium in liquid culture provides the highest level of resistance to A. flavus infection, namely 38.67% and 28.00%. The findings suggest that P. hubeiensis DP1342 has potential as a biological control agent in controlling A. flavus on nutmeg seeds, although further formulation and optimization are needed.
Co-Authors Achmad Putri, Noviani Adang Hambali, Adang Aisyah Nur Sayidatun Nisa, Aisyah Nur Al-Amin, Al-Amin Alaniri, Yusanuari Amalia, Wuliani Ani Hendriani, Ani AZMIA, SEILA Azmia, Sila Azmiyah, Mia Budi Hendrawan Carolina Sasea, Sanita Chaidar, Ana Nurganah Daradjat, Aan Andang Deisye Supit Dhemi Harlan, Dhemi Didik Harnowo Diding Nurdin, Diding Dwi N Susilowati DWI NINGSIH SUSILOWATI Fadlila, Nazli Amni Farafishah, Aisah Fathin, Muhammad Faris Fatimah Fauziah, Nurrul Hasna Fortus, Sernan Entierro Fuadah, Nayla Layinatul Gatut Wahyu Anggoro Susanto Ginanjar, Asep Hasan Basri Hidayah, Mudrikah Nurul Himmatul Ulya I Made Samudera, I Made I Made Samudra I Nyoman Widiarta Ifa Manzila Iim Siti Karimah, Iim Siti Kadir, Triny Suryani Kosasih, Jajang LAKSMI AMBARSARI Lia Dahlia, Lia Lukki Lukitawati Machmud, M Made Jana Mejaya, Made Jana Marida Santi Yudha Ika Bayu, Marida Santi Yudha Ika Nani Radiastuti Ni Luh Yulyana Dewi Nirmalasari, Cyntia Nirmalasari, Cyntia Nugroho, Eka Nur Sayidatun Nisa, Aisyah Peuo, Vibol PUJI LESTARI Radini Sinta, Radini Rismawati, Eri Rismayani Rita Hayati Rohimah , Elis Rosidah, Ros Saepudin, Ahmad Sally Badriya Hisniati Samskerta, I Putu SEMPURNA GINTING Siregar, Ruth Helena Sodik, Muhammad Sri Rahayuningsih Sri Wahyuni Indiati Suhendar, MA Supriadi Suryani Suryani Tri Puji Priyatno Trini Suryani Kadir Triny S. Kadir Trisnawati Trisnawati Trizelia . WAHID, RAHMAN Waluyo Hatmoko, Waluyo Wartono Wartono Yuliantoro Baliadi YUSMANI PRAYOGO