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Journal : BIOEDUSCIENCE

Efikasi Formulasi Granular Kombinasi Trichoderma sp. dan Ekstrak Daun Bandotan terhadap Sclerotium rolfsii pada Porang (Amorphophallus oncophyllus) Raniah, Nabilla Nur; Nirwanto, Hery; Suryaminarsih, Penta
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14880

Abstract

Background: Amorphophallus oncophyllus is a tuber plant that is beneficial for various industrial sectors, but in its cultivation, it is often affected by the pathogen Sclerotium rolfsii, requiring control measures. Control efforts can be carried out using a combination of the biological agent Trichoderma sp. and the plant pesticide made from bandotan leaf extract. The combined control application can be formulated in a solid granular form. Method: This research was conducted both in vitro and in vivo. In vitro, a Completely Randomized Design (RAL) was used with the combination of Trichoderma sp. and bandotan leaf extract, divided into four levels: 0% (TB0), 5% (TB5), 10% (TB10), and 15% (TB15). In vivo, a 2-factor RAL was applied. The first factor was the concentration of the rice flour carrier material in four levels: 0% (B1), 25% (B2), 50% (B3), and 75% (B4). The second factor was the storage duration of the granular formulation, consisting of 0 weeks (M1), 3 weeks (M2), and 5 weeks (M3). Result: The study's results showed that the granular combination of Trichoderma sp. and 10% bandotan leaf extract reduced S. rolfsii infection by 64.25% and stimulated the growth of porang plant height. Conclusion: The treatment with the granular combination of Trichoderma sp. and 10% bandotan leaf extract, adding 25% rice flour and a storage period of 0-3 weeks, showed the best effect on the porang plant seedlings.
Bio-Priming with Trichoderma spp. to Suppress Aspergillus flavus, the Causal Agent of Damping-off Disease in Peanuts Anita, Tri Yuli; Suryaminarsih, Penta; Rahmadhini, Noni
BIOEDUSCIENCE Vol 9 No 2 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14937

Abstract

Background: Aspergillus flavus is a fungus that produces aflatoxin, a toxic compound that can contaminate food products, especially nuts. The impact caused by A. flavus causes significant losses for peanut-producing countries in cases of international trade. A. flavus infection in peanuts causes physical changes and quality, as well as a decrease in seed germination. Environmentally friendly control efforts for A. flavus can be carried out by utilizing biological agents such as Trichoderma spp. This study aims to evaluate the ability of T. harzianum and T. asperellum to inhibit the growth of A. flavus. This study also evaluates bio-priming methods enriched with Trichoderma spp. to optimize peanut seed germination. Methods: The research used a factorial Completely Randomized Design (CRD) of 2 factors. The first factor is the isolate species Trichoderma spp. namely T. harzianum (T1), T. asperellum (T2), and Control (T0), namely A. flavus without biological control treatment. The second factor is the application method, which includes the bio-priming (B1) and bio-matrixpriming (B2) methods. Each treatment combination was repeated 4 times, and the number of seeds used was five in each unit. Results: T. harzianum showed the highest inhibitory ability against A. flavus in the in vitro test, namely 49%. Bio-priming treatment containing the active ingredient T. harzianum is the best treatment for increasing peanut germination by 95%. Conclusions: The research results showed that bio-priming treatment with the active ingredient T. harzianum increased the germination rate significantly compared to other treatments.
Co-Authors Abifah, Serlia Nur Agustin, Indah Sari Dwi Aisyah Lulu Hariyanto Alifta Wiranthi Anita, Tri Yuli Ardhia Pramesti Cahyani Arga Dwi Indrawan Arika Purnawati Chairunnisa Faza Nabillah Diah Budi Kusumawati Didin Julia Trisnawati Difa Eka Pranoto Dinda Rizka Rahayu Dita Megasari Dwi Lestari Ekayanti, Savira Aprilia Elly Syafriani Elvira, Nolla Dwi Erdiansyah, Mochamad Bima Erlangga Dwi Fachruddin Fadila Suryandika Ferdianto Eko Wicaksono Firdi Ihza Rochman Guniarti, Guniarti Gusdino Hasyidan Gusdino Hasyidan Haryani, Wiwik Sri Hery Nirwanto Hery Nirwanto, Hery Ida Retno Moeljani Iis Purnamawati Ika Nur Fitriana Ika Nur Fitriana Indah Sari Dwi Agustin Jati Laksono, Roy Juniawan Laksamana Agadhia Raharjo Lestari, Safira Rizka Maemunah Maemunah Maharani, Salsabilla Diva Mahfud, Nur Aini Maroeto Maroeto Moch Sodiq Nawaal, Najvania Nolla Dwi Elvira Noni Rahmadhini Nugraha Iqbal Avrianto Nur Syiami, Aning Pangesti Nugrahani Prasetyawati, Endang Triwahyu Prasetyawati, Endang Triwahyu Pribadi, Didik Utomo Putranto Sasikirono Ramdan Hidayat Raniah, Nabilla Nur Rateh Lailatul Risdiyanti Risdiyanti, Rateh Lailatul Rizky Ika Noviyanti Rosyiidah Sholihin, Alyaa Arwaa Sa'adah, Fatimah Lailatus Safira Rizka Lestari Salfi Mauludhiea Firli Serlia Nur Abifah Setyo Budi Santoso Siti Fatimatus Syahrok Sri Wiyatiningsih Sri Wiyatiningsih Syahrok, Siti Fatimatus Tri MUJOKO Ummah, Robithotul Wanti Mindari Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI