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All Journal AGRIKA Cropsaver : Journal of Plant Protection JURNAL ILMU PERTANIAN Jurnal Teknik Kimia MAPETA Agridevina : Berkala Ilmiah Agribisnis Plumula : Berkala Ilmiah Agroteknologi Jurnal Agroekoteknologi Planta Tropika BIOEDUSCIENCE Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Agrotropika Biofarm : Jurnal Ilmiah Pertanian Nusantara Science and Technology Proceedings International Journal on Food, Agriculture and Natural Resources (IJFANRES) Exact Paper In Compilation (EPIC) AGRIC Journal of Farming and Agriculture
Penta SURYAMINARSIH
UPN "Veteran" Jawa Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : bioeduscience

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Efikasi Formulasi Granular Kombinasi Trichoderma sp. dan Ekstrak Daun Bandotan terhadap Sclerotium rolfsii pada Porang (Amorphophallus oncophyllus) Raniah, Nabilla Nur; Nirwanto, Hery; Suryaminarsih, Penta
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14880

Abstract

Background: Amorphophallus oncophyllus is a tuber plant that is beneficial for various industrial sectors, but in its cultivation, it is often affected by the pathogen Sclerotium rolfsii, requiring control measures. Control efforts can be carried out using a combination of the biological agent Trichoderma sp. and the plant pesticide made from bandotan leaf extract. The combined control application can be formulated in a solid granular form. Method: This research was conducted both in vitro and in vivo. In vitro, a Completely Randomized Design (RAL) was used with the combination of Trichoderma sp. and bandotan leaf extract, divided into four levels: 0% (TB0), 5% (TB5), 10% (TB10), and 15% (TB15). In vivo, a 2-factor RAL was applied. The first factor was the concentration of the rice flour carrier material in four levels: 0% (B1), 25% (B2), 50% (B3), and 75% (B4). The second factor was the storage duration of the granular formulation, consisting of 0 weeks (M1), 3 weeks (M2), and 5 weeks (M3). Result: The study's results showed that the granular combination of Trichoderma sp. and 10% bandotan leaf extract reduced S. rolfsii infection by 64.25% and stimulated the growth of porang plant height. Conclusion: The treatment with the granular combination of Trichoderma sp. and 10% bandotan leaf extract, adding 25% rice flour and a storage period of 0-3 weeks, showed the best effect on the porang plant seedlings.
Bio-Priming with Trichoderma spp. to Suppress Aspergillus flavus, the Causal Agent of Damping-off Disease in Peanuts Anita, Tri Yuli; Suryaminarsih, Penta; Rahmadhini, Noni
BIOEDUSCIENCE Vol 9 No 2 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14937

Abstract

Background: Aspergillus flavus is a fungus that produces aflatoxin, a toxic compound that can contaminate food products, especially nuts. The impact caused by A. flavus causes significant losses for peanut-producing countries in cases of international trade. A. flavus infection in peanuts causes physical changes and quality, as well as a decrease in seed germination. Environmentally friendly control efforts for A. flavus can be carried out by utilizing biological agents such as Trichoderma spp. This study aims to evaluate the ability of T. harzianum and T. asperellum to inhibit the growth of A. flavus. This study also evaluates bio-priming methods enriched with Trichoderma spp. to optimize peanut seed germination. Methods: The research used a factorial Completely Randomized Design (CRD) of 2 factors. The first factor is the isolate species Trichoderma spp. namely T. harzianum (T1), T. asperellum (T2), and Control (T0), namely A. flavus without biological control treatment. The second factor is the application method, which includes the bio-priming (B1) and bio-matrixpriming (B2) methods. Each treatment combination was repeated 4 times, and the number of seeds used was five in each unit. Results: T. harzianum showed the highest inhibitory ability against A. flavus in the in vitro test, namely 49%. Bio-priming treatment containing the active ingredient T. harzianum is the best treatment for increasing peanut germination by 95%. Conclusions: The research results showed that bio-priming treatment with the active ingredient T. harzianum increased the germination rate significantly compared to other treatments.
Exploration and Morphological Characterization of Non-pathogenic Streptomyces sp. from Mangrove Rhizosphere Ecosystem Aisah, Frida Nur; Suryaminarsih, Penta; Wuryandari , Yenny
BIOEDUSCIENCE Vol 10 No 2 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18986

Abstract

Background: Streptomyces spp. are well known for their ability to produce antibiotic compounds with potential applications in plant disease management, but research in mangrove ecosystems tends to be limited. This study aims to explore and characterize Streptomyces isolates from the mangrove rhizosphere based on morphological characteristics and pathogenicity properties to find candidates as biocontrol agents. Methods: Morphological characterization was carried out macroscopically and microscopically, while hypersensitivity tests and soft rot tests were carried out to determine non-pathogenic properties. Results: A total of 24 isolates of Streptomyces sp. were successfully isolated with the morphological characteristics of circular colonies, filamentous and curved colony edges, convex, flat, raised, and umbonate colony elevations, aerial mycelial pigmentation with six color variations, and substrate mycelial pigmentation with six color variations. Microscopically, two types of spore chains were obtained, namely open spiral and closed spiral. All isolates were nonpathogenic based on the negative results of the hypersensitivity test and soft rot test. Conclusion: The mangrove rhizosphere provides a promising and morphologically diverse source of non-pathogenic Streptomyces isolates. Identification in this study was limited to morphological characterization; therefore, molecular analysis is needed to confirm the isolate species. These findings provide preliminary information as a basis for selecting isolates for further biological control studies.
Co-Authors Abifah, Serlia Nur Agustin, Indah Sari Dwi Aisah, Frida Nur Aisyah Lulu Hariyanto Alifta Wiranthi Anita, Tri Yuli Ardhia Pramesti Cahyani Arga Dwi Indrawan Arika Purnawati Chairunnisa Faza Nabillah Diah Budi Kusumawati Didin Julia Trisnawati Difa Eka Pranoto Dinda Rizka Rahayu Dita Megasari Dwi Lestari Ekayanti, Savira Aprilia Elly Syafriani Elvira, Nolla Dwi Erlangga Dwi Fachruddin Ferdianto Eko Wicaksono Firdi Ihza Rochman Guniarti, Guniarti Gusdino Hasyidan Gusdino Hasyidan Harsiyanti, Harsiyanti Haryani, Wiwik Sri Hery Nirwanto Hery Nirwanto, Hery Ida Retno Moeljani Iis Purnamawati Ika Nur Fitriana Ika Nur Fitriana Indah Sari Dwi Agustin Jati Laksono, Roy Juniawan Laksamana Agadhia Raharjo Maharani, Salsabilla Diva Maroeto Maroeto Moch Sodiq Nawaal, Najvania Nolla Dwi Elvira Noni Rahmadhini Nugraha Iqbal Avrianto Nur Syiami, Aning Pangesti Nugrahani Prasetyawati, Endang Triwahyu Pratista, Ariq Dies Pribadi, Didik Utomo Putranto Sasikirono Ramdan Hidayat Raniah, Nabilla Nur Rateh Lailatul Risdiyanti Risdiyanti, Rateh Lailatul Rizky Ika Noviyanti Rosyiidah Sholihin, Alyaa Arwaa Sa'adah, Fatimah Lailatus Safira Rizka Lestari Salfi Mauludhiea Firli Serlia Nur Abifah Setyo Budi Santoso Siti Fatimatus Syahrok Siti Fatimatus Syahrok Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Suryandika, Fadila Syahrok, Siti Fatimatus Tri MUJOKO Ummah, Robithotul Wanti Mindari Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Wuryandari , Yenny Yenny WURYANDARI