Article Per Year (5 Year)
p-Index From 2021 - 2026
2.195
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Journal of Biomedicine and Translational Research Research Journal of Life Science Jurnal Penelitian Pendidikan IPA (JPPIPA) International Journal of Community Service Learning ISM (Intisari Sains Medis) : Jurnal Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Caring : Jurnal Pengabdian Masyarakat Jurnal Klinik dan Riset Kesehatan MEDICINUS The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Folia Medica Indonesiana
Hani Susianti
Department Of Clinical Pathology Faculty Of Medicine Universitas Brawijaya Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

Published : 25 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : Research Journal of Life Science

 1 
Development of Candidate Antigens for Rapid Test Kit to Detect Autoantibodies in Patients with Systemic Lupus Erythematosus Wisnu Barlianto; Hani Susianti; Singgih Wahono; Nelly Ismayasih; Rossy Meilani; Kusworini Handono
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.941 KB) | DOI: 10.21776/ub.rjls.2017.004.01.8

Abstract

Systemic Lupus Erythematosus (LES) is an autoimmune inflammatory disease characterized by the formation of anti-nuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) antibodies as diagnostic markers. Detection of such autoantibodies requires advanced equipment and trained personnel.This study was conducted to acquire candidate antigens that can be used for rapid test kit for practical and accurate detection of ANA and anti-dsDNA to speed up SLE diagnosis.Nuclear proteins and DNA derived from cell lines, hair follicles, and leukocytes of SLE patients and healthy individuals were isolated using QiaGEN kit and modified-manual procedure. Antigen-antibody bonds were tested by dot blot assay.The strongest binding between DNA antigens of a healthy individual and antibodies occurred at dilution factors of 1:5,120 for the antigen and 1:2,560 for the antibody. The strongest binding between nuclear protein antigens from the cell line and antibodies occurred at dilution factors of 1:512 for the antigen and 1:1,600 for the antibody.Nuclear antigens derived from cell line and DNA antigens of healthy individuals were antigen candidates for the development of ANA and anti-dsDNA rapid detection tests.
Evaluation of Urine Procalcitonin, Interleukin-6, Heparin Binding Protein, Leukocyturia, and Bacteriuria for Early Detecting Urinary Tract Infection Hani Susianti; I Putu Adi Santosa; Antonius Johanes; Esther Mayrita; Thiba Dina Merdikaningsih
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.2

Abstract

Urinary Tract Infection (UTI) is a common infectious disease. Urine culture as a gold standard is less practical and time- consuming. Examination of urine dipstick and microscopic is less accurate. Procalcitonin (PCT), IL-6 and Heparin Binding Protein (HBP) have known increase after infection, which potentially used for early detection of UTI. The aim of this study was to determine the diagnostic performance of urine IL-6, HBP, PCT, leukocyturia and bacteriuria level as biomarkers of UTI. The study was conducted on 51 UTI patients with positive urine culture, 16 UTI patients with negative urine culture, and 16 healthy control.  Urine IL-6, HBP, and PCT level were determined using ELISA. Leukocyturia and bacteriuria were measured using automated flowcytometry and fluorescent dye analyzers.  The gold standard for UTI diagnosis was urine culture. The sensitivity and specificity of urine PCT, IL-6, HBP, leukocyturia and bacteriuria were 60.78% and 65.62%; 72.55% and 56.25%; 96.07% and 50.00%; 80.39% and 78.12%; 80.39% and 59.09% respectively. The AUC of urine PCT, IL-6, HBP, leukocyturia and bacteriuria were 0.600; 0.605; 0.666; 0.877 and 0.830 respectively. For combinations of two biomarkers, the best sensitivity and specificity was demonstrated by urine PCT and leukocyturia.
Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis Hani Susianti; Wisnu Barlianto; Dian Sukma Hanggara; Kusworini Handono; Purwanto Adipireno; Lisyani Suromo
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.828 KB) | DOI: 10.21776/ub.rjls.2017.004.01.6

Abstract

Background. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together with alpha-1 antitrypsin (AAT), they are expected to become new biomarkers to assess LN activity.Method. Seventy-one urine samples were collected from healthy controls and LN patients. Patterns of urine specific proteins were determined using column chromatography and SDS-PAGE tests, LN activity was calculated using SLEDAI-renal domain score, and AAT concentrations was measured by ELISA.Result. The majority of proteins in the control group have molecular weights of >66 kDa (88%) and 21- to 25-kDa proteins were observed only in the case group. The p values for differences in urine AAT concentration between active LN and healthy controls, inactive LN and healthy controls, and active LN and inactive LN were 0.004, 0.046, and 0.054, respectively, whereas those for urine AAT/creatinine ratio were 0.489, 0.019, and 0.915, respectively.Conclusion: There were differences in the patterns of the molecular weight of proteins and urine AAT concentrations between case group and control group. However, no such differences were identified between active and inactive LN. 
Co-Authors Abd. Rasyid Syamsuri Ade Wildan Rizky Fachry Agustina Tri Endharti Airlangga, Dimas Ikhsan Anik Widijanti Antonius Johanes Ati Rastini Atma Gunawan Atma Gunawan Atma Gunawan Bayu Eka Nugraha Bogi Pratomo Bogi Pratomo Bogi Pratomo Choirinnisa Meilia Ayu Putri Chomsin Sulistya Widodo Dany Farida Deasy Ayuningtyas Tandio Deasy Ayuningtyas Tandio desy wulandari Dewi Purnama Sari Dian Hasanah Dian Sukma Hanggara Dian Sukma Hanggara Dian Sukma Hanggara Dimas Ikhsan Airlangga Dominica Pita Sari Dwi Priyadi Djatmiko Eko, Mudjiwijono Handaru Ekowati Retnaningtyas Elvin Richela Lawanto Elvira Sari Dewi Emanuel Hananto Engli, Katherina Engli, Katherina Alfa Esther Mayrita Gonius, Andry Handono Kalim Handono Kalim Handono Kalim Handono Kalim Hari Oki, Hambiah I Komang Adi Widana I Putu A.S I Putu Adi Santosa I Putu Adi Santosa Indah Adhita Wulandari Intanwati, Sherly Kalsumy, Umi Katherina Engli Kusworini Handono Kusworini Handono Kusworini Handono Kusworini Handono Kusworini Handono Kusworini Handono Laura Christiani Levrita Nindya Poetri Lisyani Suromo Meilani, Rossy Mona Yuliari Mudjiwijono Handaru Eko Muhammad Anshory, Muhammad Nelly Ismayasih Nimas Eka Firdaningrum Nora Ariyanti Norma Hanifah Sumarta Nugraha Setiawan Nugraha Setiawan Nugraha, Bayu Eka Nurdiana Nurdiana Nurdiana Nurdiana Perdana Aditya Rahman Pratama, Mirza Zaka Purwanto Adipireno Putri, Laily Ardhianti Putu Adi Santosa, Putu Adi R.I R.I Rahmawati, Hanifa Rizky Raymond Poeng Rossy Meilani Sari, Atika Windra Sari, Tita Luthfia Shahdevi Nandar Kurniawan, Shahdevi Nandar Singgih Wahono Siti Roziah Ria Famuji Solly Aryza Susanto Nugroho Syahrul Chilmi Syahrul Chilmi, Syahrul Thiba Dina Merdikaningsih Torajasa Achamar Tri Wahyudi Iman Dantara Wahono, Cesarius Singgih Wisnu Barlianto Wisnu Barlianto Wisnu Barlianto Yuliana Salman