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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Akademika Biologi Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) International Journal of Agriculture System Indonesian Journal of Forestry Research
Teuku Tajuddin
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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Journal : International Journal of Agriculture System

 1 
Comparison of DNA Isolation Methods that Derived from Leaves of a Potential Anti-Cancer Rodent Tuber (Typhonium flagelliforme) Plant Gemilang Rahmadara; Nurul Fitri Hanifah; Rismayanti Rismayanti; Devit Purwoko; Andi Rochandi; Teuku Tajuddin
International Journal of Agriculture System VOLUME 10 ISSUE 2, DECEMBER 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v10i2.3966

Abstract

The content of polysaccharides, polyphenols, proteins, and RNA compounds is the main problem often found in Plants DNA isolation, which inhibit the process of DNA isolation. Comparing the methods of plant DNA isolation is necessary for obtaining the DNA with good quality, purity, high concentration and efficiency time and cost. This study aimed to determine the best DNA isolation method that derived from leaves of a potential anti-cancer Rodent Tuber (Typhonium flagelliforme) plant by comparing the conventional DNA isolation method (cetyl trimethyl ammonium bromide/CTAB) and 2 commercial kits (Promega Wizard™ Genomic DNA Purification Kits, and Geneaid Genomic Mini Kit). The results showed that the CTAB method yielded a higher amount of DNA (>100 ng/µL) at the cost of 0.49 USD per sample, in comparison with Promega method (69.19 to 157.68 ng/µL) at 3.28 USD per sample and Geneaid method (8.15 to 18.52 ng/µL) at 2.06 USD per sample. Based on the purity of isolated DNA (A260/280), CTAB method produced relatively similar DNA quality to Promega kit (1.8-2.0). On the other hand, Geneaid method resulted in a lower purity value at 1.15 to 1.60.
The Graded Concentration of Bleach Solution and Material Size Plays an Important Role in the In Vitro Sterilization of Thyphonium flagelliforme Plants Rahmadara, Gemilang; Tajuddin, Teuku; Suparjo, Suparjo; Sobir, Sobir; Dinarti, Diny; Khairiyah, Hayat
International Journal of Agriculture System VOLUME 12 ISSUE 1, JUNE 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v12i1.5070

Abstract

The sterilization is a primary step in in-vitro culture. plant breeding through in-vitro culture hinges on the successful effectiveness of the sterilization method in producing sterile plants. This research aims to optimize sterilization methods using a completely randomized factorial design. The first factor is the size of the material (small and large), and the second factor is the graded concentration of the bleach solution. The study revealed that Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants. The application of graded bleach solution concentrations at 25%, 20%, and 15% is able to reduce contamination to 0% on small size materials and 5 % on large size materials. Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants.
The Graded Concentration of Bleach Solution and Material Size Plays an Important Role in the In Vitro Sterilization of Thyphonium flagelliforme Plants Rahmadara, Gemilang; Tajuddin, Teuku; Suparjo, Suparjo; Sobir, Sobir; Dinarti, Diny; Khairiyah, Hayat
International Journal of Agriculture System VOLUME 12 ISSUE 1, JUNE 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v12i1.5070

Abstract

The sterilization is a primary step in in-vitro culture. plant breeding through in-vitro culture hinges on the successful effectiveness of the sterilization method in producing sterile plants. This research aims to optimize sterilization methods using a completely randomized factorial design. The first factor is the size of the material (small and large), and the second factor is the graded concentration of the bleach solution. The study revealed that Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants. The application of graded bleach solution concentrations at 25%, 20%, and 15% is able to reduce contamination to 0% on small size materials and 5 % on large size materials. Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants.
Co-Authors . Suparjo, . . Supriyanto Abd. Rasyid Syamsuri AGUS SUYONO Ahmad Riyadi Andi Rochandi Anna Safarrida, Anna Anto Budiharjo Bedah Rupaedah, Bedah Cartealy, Imam Cartealy, Imam Cartealy, Imam Civi Cartealy, Imam Civi damayanti, dini Devit Purwoko Diny Dinarti Erwinda . Evawati Evawati Fadhullah, Hafizh Farida Rosana Mira, Farida Rosana Fauzan, Yusuf Sigit A. Fauzan, Yusuf Sigit Ahmad Fauzan, Yusuf Sigit Ahmad Gemilang Rahmadara Hanifah, Nurul Fitri Hartuti, Endah Dwi Hermin Pancasakti Kusumaningrum Irwan Roza Joko Mulyono Juanda ., Juanda Juwartina Ida Royani, Juwartina Ida Karyanti ., Karyanti Karyanti Karyanti, Karyanti Karyanti, . Karyanti, . Khairiyah, Hayat Khairiyah, Hayat Kitagawa, Sayuri Mahmud Sugianto, Mahmud MEITY SURADJI SINAGA Minaldi ., Minaldi Nadirman Haska Nurul Fitri Hanifah Pramudyawardhani, Okky Dwi Pramudyawardhani, Okky Dwi Purwoko, Devit purwoko, devit Rahmadara, Gemilang Reninta, Rikania Rismayanti Rismayanti Rudiyana, Yayan Saga, Hirohisa Siti Zulaeha, Siti Sobir Sobir Sri Wilarso Budi Sudarsono Sudirman Yahya Sudjai, Imam Suharsono Suharsono Suparjo Suparningtyas, Juniza Firdha Supriyanto SYOFI ROSMALAWATI Tati Sukarnih, Tati Wahono Sumaryono YENNI BAKHTIAR Yohanes Chandrawijaya Yusuf Sigit, Yusuf