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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan
Agnesia Endang Tri Hastuti Wahyuni
Departemen Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Combination of Wild ginger, Honey, and Probiotics as Growth Prometer Candidates : An in Vitro Study Marlin Cindy Claudya Malelak; Agnesia Endang Tri Hastuti Wahyuni; Agustina Dwi Wijayanti; Vinsa Cantya Prakasita
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53256

Abstract

Growth Promoter Antibiotics are used to prevent disease and promote growth and production in poultry. Repeated admnistration of feed can have a microorganic resistance effect, accumulation of antibiotic residues in animal and environmental products and imbalance of normal microflora in the intestine. The antibacterial and carbohydrate content of some natural ingredients can be potential as a replacement candidate for AGP. This study aims to determine the role of a combination of wild ginger, honey, and probiotics (Bacillus subtilis and Lactobacillus acidophilus) as AGP candiate in Vitro. The antibacterial activity of the combination of wild ginger and honey against pathogens (E. coli) and their use against probiotics was tested by disk diffusion method, while the calculation of optical density values to determine the minimum inhibitory concentration and minimum bactericidal concentration was carried out on E. coli. The ability of inhibition of probiotics against pathogens is also done by the disk diffusion method. The disk diffusion test results showed the best combination was extract of 25% wild ginger aquades + 100% Lombok honey with inhibition zone diameter (8,53 ±, 03). Optical density values indicate this combination is able to inhibit and kill E. coli (DO 0,00 ±, 002) and support B. subtilis (DO 0,18 ±, 002) and L. acidophilus (DO 0.25 ±, 005) significantly better than positive control. MIC value of wild ginger aquades extract and honey combination against E. coli is wild ginger aquades extract 3.13% + Lombok honey 25%, while MBC value is wild ginger aquades extract 6,25% + Lombok honey 25%. The combination of B. subtilis and L. acidophilus showed the largest inhibitory zone diameter against E. coli (7,30 ±, 02 mm) compared to individual colonies. The combination of wild ginger and honey, in addition to inhibiting also able to kill pathogens and support the growth of probiotics, so this formula can be used as one of the replacement candidates for AGP.  
ANALISIS MIKROBIOLOGI SUSU KAMBING PERANAKAN ETTAWA (PE) DARI KABUPATEN SLEMAN YOGYAKARTA Widodo Suwito; Widagdo Sri Nugroho; AETH Wahyuni Wahyuni; Bambang Sumiarto
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.189 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2625

Abstract

Tujuan penelitian ini mengetahui kualitas mikrobiologi susu kambing mentah yang diambil langsung dari ambing. Dalam penelitian ini digunakan 50 sampel susu kambing peranakan Ettawa (PE). Sampel dianalisis terhadap total plate count (TPC), jumlah Staphylococcus sp., jumlah koliform, Escherichia coli (E. coli), dan Salmonella sp. berdasarkan reaksi biokimia. Rerata untuk TPC; Staphylococcus sp.; total koliform masing-masing adalah 1,65x10 3 ; 5,75x10 3 ; 1,3x10 cfu/ml, sedangkan E. coli dan Salmonella sp. adalah negatif. Berdasarkan Standar Nasional Indonesia (SNI) No 01-6366-2000 tentang persyaratan susu segar, maka TPC, koliform, E. coli, dan Salmonella sp. memenuhi standar, sedangkan Staphylococcus sp. melebihi ambang batas. Hasil penelitian menunjukkan bahwa susu kambing mentah yang diambil langsung dari ambing masih layak konsumsi.
EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo; Rahmat Setya Adji
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.909 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2802

Abstract

Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua  menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.
EVALUASI KIT DETEKSI CEPAT TERHADAP SAMPEL OTAK ANJING TERINFEKSI VIRUS RABIES Michael Haryadi Wibowo; Tri Untari; Sidna Artanto; Surya Amanu; AETH. Wahyuni; Widya Asmara
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.545 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2797

Abstract

Penelitian ini bertujuan mengetahui potensi kit deteksi cepat Anigen® rapid test kit rabies Ag dalam mendeteksi virus rabies pada sampel otakanjing yang diperoleh dari lapangan yang meliputi batas deteksi, kecepatan reaksi, uji reaksi silang, uji sensitivitas, dan spesifisitas. Batas deteksi ditentukan dengan pengenceran secara serial kontrol positif virus rabies dan selanjutnya diuji dengan rapid test kit sesuai petunjuk produsen. Uji reaksi silang dilakukan dengan canine parvovirus, Escherichia coli, dan Salmonella sp. Uji sensitivitas dan spesifisitas dilakukan terhadap sampel otak yang telah dikonfirmasi positif rabies dengan uji fluorescent antibody technique. Konfirmasi uji rapid test tersebut dilakukan dengan reverse transcriptase polymerase chain reaction. Berdasarkan data yang diperoleh dalam penelitian ini dapat disimpulkan bahwa Anigen® rapid test kit rabies Ag mampu mendeteksi sampel yang mengandung virus rabies dengan titer 0,5 x log 106,5/0,03 ml, dengan rata-rata kecepatan reaksi 1,8 menit 29,35 detik (kurang dari 2 menit). Di samping itu Anigen® rapid test kit rabies menunjukkan tidak terdapat reaksi silang dengan canine parvovirus, Escherichia coli, dan Salmonella sp. serta mempunyai sensitivitas 92,30% dan spesifisitas 97,90%
Aktivitas Antibakteri Ekstrak Daun Pepaya, Daun Kemangi Serta Temu Ireng, dan Madu terhadap Bakteri Serratia marcescens Agnesia Endang Tri Hastuti Wahyuni; Yovita Devina; Vinsa Cantya Prakasita; Dwi Cahyo Budi Setiawan
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.465

Abstract

Antibiotic Growth Promoters (AGPs) are antibiotics that are used commonly in livestock farming to increase animals growth rate. The use of AGP has been prohibited in Indonesia (No.14/PERMENTAN/PK.350/5/2017). AGP banning urges some innovations to find the alternative of AGP and one of them is utilization of natural resources. Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey contain flavonoid that has antibacterial activity. The purpose of this research is to know the effect of Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey against Serratia marcescens growth. Re-identification of Serratia marcescens were done by looking at the colony morphology, cell morphology and biochemical tests. Antibacterial activity of ethanol (100%) and aquades (33.33%) extract of the herbals and the honey (100%) against Serratia marcescens were tested by disc diffusion method. Each test was repeated 2 times. The results showed that Lanceng (Trigona bee) honey from Gunung Kidul, Black honey from Lombok, White honey from Lombok, ethanol and aquades extract of the herbals are not effective to inhibit Serratia marcescens growth. Commercial honey (7.59±.22 mm) has the highest antibacterial acivity to Serratia marcescens, followed by honey from Kupang (6.69±.21 mm). Commercial honey and honey from Kupang have moderate antibacterial activity.
Detection of Newcastle Disease, Avian Influenza, dan Infectious Bronchitis Virus from Commercial with Respiratory Clinical Signs Santoso, Ferdinand Prayogo Cahyo; Wahyuni, Agnesia Endang Tri Hastuti; Untari, Tri; Asmara, Widya; Wibowo, Michael Haryadi
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.93221

Abstract

Clinical symptoms in the chicken’s respiratory system, such as sores, nasal discharge, gasping, and so on, can be caused by a variety of possible socks. These signs may be caused by a virus, usually followed by economic losses. Causative diagnosis, especially virus detection, is needed to establish the cause of the symptoms. The study aims to detect Newcastle Disease (ND), Avian Influenza (AI), and Infectious Bronchitis (IB) cases of respiratory symptoms in commercial chickens by 2023. The samples studied in this study were organs from commercial chicken broilers and layers with respiratory symptoms. The organ was prepared as a suspension, then centrifuged to take out the supernatants. Supernatants were extracted using commercial extraction kits to produce templates for molecular detection. Molecular detection was performed using the RT-PCR two-step method. Primer pairs were used for the detection of ND, AI subtypes H5 and H9, as well as IB. The results were visualized by electrophoresis with 1.5% agarose. Interpretation of the positive result of RT-PCR based on the appearance and length of amplicons compared with the positive control of each virus. Positive RT-PCR samples with thick amplicon quality were subjected to sequencing and bioinformatics analysis. Results of detection with RT-PCR against 41 samples are positive ND of 1 out of 8 samples (12.5%), AI of 2 out of 17 samples (11.7%), and IB of 5 out of 16 samples (31.3%). Based on the molecular detection with RT-PCR, the symptoms of respiration in commercial chickens are confirmed due to the cause, namely the virus: ND genotype VII-i, AI subtype H5 clade 2.3.2, AI subtype H9, and IB genotype GI-19 (QX-like).
EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX Ebenhaizar Sanam, Maxs Urias; Asmara, Widya; Tri Hastuti Wahyuni, Agnesia Endang; Wibowo, Michael Haryadi; Adji, Rahmat Setya
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2802

Abstract

Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.
Co-Authors Adrenalin, Sruti Listra Agustina Dwi Wijayanti Anak Agung Gede Sugianthara Arfian Rahma Shanti Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabu Dito Anggoro Ery Haryani Fauziah, Ima Herawati, Okti Hilda Susiyanti Debora Berek I wayan Teguh Wibawan Ima Fauziah Ima Fauziah Imanjati, Lynda Nugrahaning Liza Angeliya Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Maria Anggita Marla Anggita Marlin Cindy Claudya Malelak Marseni Banne Ringgi Maxs Urias Ebenheizer Sanam Michael Haryadi Wibowo Nursyirwani, Nursyirwani Nyoman Reishita Andriyani Pasha Glabella Radhian Fadiar Rahmat Setya Adji Rahmat Setya Adji Salma Ramandhanti Putri Santoso, Ferdinand Prayogo Cahyo Setiawan, Dwi Cahyo Budi Setyawan Budiharta Sidna Artanto Sidna Artanto Sidna Artanto Sitarina Widyarini Sitarina Widyarini Sruti Listra Adrenalin Sruti Listra Adrenalin Surya Amanu Surya Amanu Tangkonda, Elisabet Tati Aryani Tri Untari Tri Untari Triyanto . Vinsa Cantya Prakasita Widagdo Sri Nugroho Widagdo Sri Nugroho Widodo Suwito Widodo Suwito Widodo Suwito Widya Asmara Widya Asmara Yovita Devina Yovita Devina