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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan
Agnesia Endang Tri Hastuti Wahyuni
Departemen Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Isolasi, Identifikasi, dan Serotyping Avibacterium paragallinarum dari Ayam Petelur Komersial yang Menunjukkan Gejala Snot Elisabet Tangkonda; Charles Rangga Tabbu; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.293 KB) | DOI: 10.22146/jsv.48489

Abstract

Infectious coryza (snot) is one of acute respiratory disease in breeders, layers, and broilers caused by Avibacterium paragallinarum (Av. paragallinarum). This disease is very harmful because of its cause decrease in egg production and high morbidity. Snot incident in Indonesia still is reported. Vaccination is one of the best preventive measures, but reports about Av. paragallinarum serotype at the field so lack so that the correspondence between serotype Av. paragallinarum in the field to those used for vaccination is unknown. Av. paragallinarum has strains with different antigenicity and until now known three serotypes there are serotypes A, B and C. Serotypes A and C are pathogenic then serotypes B. The purpose of this study was to isolate, identify and to determine the serotype of Av. paragallinarum from the commercial layer that showing symptoms of snot. Samples were taken from layer which showed symptoms of snot (nasal foul smelling exudate, infra-orbital and wattle swelling, conjunctivitis, and snoring) from some layer farms. Samples were cultured on chocolate agar and then incubated in a candle jar at 37 ᴼC for 18-24 hours. Bacteria colony and cell morphology were observed and performed biochemical tests (catalase, oxidase, urease, indole, and fermentation of carbohydrates) in suspected colonies. Serotypes test was conducted using plate agglutination test (PAT). This study revealed 4 isolates Av. paragallinarum with 2 isolates are serotype B and 2 other are serotype C.
Protektivitas Sapi di Kabupaten Kupang Terhadap Penyakit Ngorok (Septicaemia Epizootica) (PROTECTIVITY AGAINST SEPTICAEMIA EPIZOOTICA OF COWS IN KUPANG DISTRICT) Hilda Susiyanti Debora Berek; Widagdo Sri Nugroho; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Controlling SepticemiaEpizooticae (SE) through vaccination program has been undertaken in KupangDistrict. However, numbers of fatal cases is still being reported. A cross sectional study have been undertakenin order to determine the prevalence and factors affecting herd immunity at farm level in Kupang District.A total of 434 cow’s sera from 87 farmers were collected and further analyzed using Enzyme-linkedImmunosorbent Assay (ELISA). An antibody titer of e”200 ELISA Unit was used as the indicator ofprotective immunity. The prevalence of herd immunity was 73.7% and vaccination coverage was 90.8%.Based on unweighted logistic regression analysis it was found that factors affecting the animals protectiveimmunity were: animal >2 years of age (r = +1.45601; OR = 4.3); housing system ( r = +1.03958; OR = 2.8);reservoir animals (r = +0.090147; OR = 2.5), the sex of the animal (female) (r = + 0.080138; OR =2.2); oncefrequency of vaccination (r = +0.61015; OR = 1.8); and 6-12 months post vaccination period (r = + 0.58968;OR = 1.8). Based on linier regression analysis, the prevalence of herd immunity was increased during rainyseason, whereas it was decreased when cows werebought from animal market or from other district.
Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA) Widodo Suwito; Widagdo Sri Nugroho; Bambang Sumiarto; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated  Bacillus sp  (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).
Aktivitas Antibakteri Ekstrak Daun Pepaya, Daun Kemangi Serta Temu Ireng, dan Madu terhadap Bakteri Serratia marcescens (ANTIBACTERIAL ACTIVITY OF PAPAYA LEAVES, BASIL LEAVES AND CURCUMA AERUGINOSA EXTRACT AND HONEY AGAINST SERRATIA MARCESCENS) Yovita Devina; Vinsa Cantya Prakasita; Dwi Cahyo Budi Setiawan; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Antibiotic Growth Promoters (AGPs) are antibiotics that are used commonly in livestock farming to increase animals growth rate. The use of AGP has been prohibited in Indonesia (No.14/Permentan/PK.350/ 5/2017). AGP banning urges some innovations to find the alternative of AGP and one of them is utilization of natural resources. Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey contain flavonoid that has antibacterial activity. The purpose of this research is to know the effect of Papaya leaves, basil leaves, Curcuma aeruginosa rhizomes and honey against Serratia marcescens growth. Re-identification of Serratia marcescens were done by looking at the colony morphology, cell morphology and biochemical tests. Antibacterial activity of ethanol (100%) and aquades (33.33%) extract of the herbals and the honey (100%) against Serratia marcescens were tested by disc diffusion method. Each test was repeated 2 times. The results showed that Lanceng (Trigona bee) honey from Gunung Kidul, Black honey from Lombok, White honey from Lombok, ethanol and aquades extract of the herbals are not effective to inhibit Serratia marcescens’s growth. Commercial honey (7.59±0.22 mm) has the highest antibacterial acivity to Serratia marcescens, followed by honey from Kupang (6.69±0.21 mm). Commercial honey and honey from Kupang have moderate antibacterial activity. It can be cocluded that comercial honey and honey from Kupang can ihibit Serratia marcescens’s growth
Analisis Genetik Gen Protective Antigenic pada Bacillus anthracis Isolat Jawa Tengah dan Yogyakarta (GENETIC ANALYSIS ON PROTECTIVE ANTIGENIC GENE OF BACILLUS ANTHRACIS ISOLATES OF CENTRAL JAVA AND YOGYAKARTA) Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to determine sequence and genotype diversity of protective antigenic gene ofBacillus anthracis isolated from Central Java and Yogyakarta. Pag-A gene which encodes for antigenicprotein is one toxin component and the virulent factor of B. anthracis. As many as five isolates fromSemarang, Sragen, and Boyolali (Central Java) and Sleman (Yogyakarta) were used. The gene wassequenced and amplified used three set of primers PA1857/PA2436, PA8/PA5, and PA-5F/PA-5R. Theresult showed that the nucleotide sequences of gene from five isolates were identical and only had onenucleotide difference as compared to B. anthracis sterne M22589. All isolates were confirmed as genotypebased on pag-A sequence. It was concluded that all B. anthracis from Central Java and Yogyakarta haveidentical pag-A sequence and belong to genotypt-1. Further studies are needed to investigate B. anthracisisolates from other regions of Indonesia.
Resistansi Antibiotik Bakteri dari Ulas Kloaka Burung Puyuh Sehat Maria Anggita; Widya Asmara; Tri Untari; Michael Haryadi Wibowo; Sidna Artanto; Okti Herawati; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.172 KB) | DOI: 10.19087/jveteriner.2021.22.4.508

Abstract

Anti Mikrob Resistan (AMR) menjadi masalah utama baik pada manusia, hewan, dan lingkungan. Pada umumnya pemberian antibiotik dilakukan oleh peternak unggas di Indonesia melalui pakan sebagai antibiotik pemacu pertumbuhan/Antibiotic Growth Promoters (AGP). Penggunaan antibiotik yang berlebihan di industri peternakan dianggap berkontribusi terhadap meningkatnya resistansi obat pada manusia. Penelitian ini bertujuan untuk mengetahui kejadian resistansi bakteri dari ulas/swab kloaka burung puyuh sehat. Sebanyak sepuluh ekor puyuh sehat dari peternakan puyuh di daerah Kalasan, Klaten, Yogyakarta diswab kloaka dan dikultur pada media cair Brain Heart Infusion (BHI). Biakan ditanam pada media Mueller Hinton Agar (MHA) dan diletakkan Sembilan jenis cakram/disk antibiotik: streptomisin, doksisiklin, fosfomisin, kloramfenikol, kolistin, siprofloksasin, ampisilin, eritromisin, dan penisilin. Setelah inkubasi pada suhu 37°C selama 18-24 jam, zona hambat yang terbentuk kemudian diukur dan ditentukan sifat resistansi dibandingkan dengan standar. Hasil menunjukkan sebanyak 20%kultur bakteri resistan terhadap streptomisin, 40% resistan terhadap doksisiklin, 40% resistan terhadap kloramfenikol, 50% resistan terhadap kolistin, 20% resistan terhadap siprofloksasin, 20% resistan terhadap ampisilin, 90% resistan terhadap eritromisin, 50% resistan terhadap penisilin, dan tidak ada resistansi terhadap fosfomisin. Terdapat satu dari sepuluh puyuh (P10) yang memiliki resistansi terhadap tujuh dari sembilan jenis antibiotik (78%) yang diujikan, dan dua dari sepuluh puyuh (P2 dan P4) memiliki resistansi terhadap dua dari sembilan antibiotik (11%). Hasil penelitian menunjukkan bahwa bakteri dari swab kloaka pada burung puyuh sehat umur 21 hari dari satu peternakan yang sama memiliki tingkat resistansi yang berbeda-beda. Sifat resistansi terhadap antibiotik dari masing-masing puyuh juga berbeda-beda.
Histopatologi Ikan Kerapu Macan yang Diimbuhi Bakteri Asam Laktat dan Diuji Tantang Vibrio alginolyticus (HISTOPHATOLOGY OF TIGER GROUPER SUPPLEMENTED WITH LACTIC ACID BACTERIA AND CHALLENGED BY VIBRIO ALGINOLYTICUS) Nursyirwani .; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Triyanto .
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.771 KB)

Abstract

Supplementation of lactic acid bacteria (LAB) as probiotic in aquaculture has been reported toincrease fish growth and enhance their resistance against diseases. The aim of this study was to figureout histological changes of tiger grouper (Epinephelus fuscoguttatus) fed with LAB isolates followed bycha
Uji Kepekaan Avibacterium paragallinarum Terhadap Antibiotik yang Berbeda Elisabet Tangkonda; Charles Rangga Tabu; Agnesia Endang Tri Hastuti Wahyuni
JURNAL KAJIAN VETERINER Vol 2 No 1 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i1.987

Abstract

Avibacterium paragallinarum is the aetiology of Infectious coryza (snot), one of acute upper respiratory tract diseases, which causes financial loss due to the decrease of egg production in layer. This is Gram negative bacteria which have three serotypes, known as serotype A, B, and C. The aim of this research was to know the sensitivity of Avibacterium paragallinarum to different kinds of antibiotics that are commonly used. This research used Avibacterium paragallinarum culture derived from layer which showed the symptoms of snot. Sensitivity test applied erythromycin, enrofloxacin, gentamycin, amoxicillin and clavulanic acid, and sulfamethoxazol. Results found the sensitivity of Avibacterium paragallinarum to combination of amoxicillin and clavulanic acid was 100%, to erythromycin 50% resistant and 50% intermediate, to enrofloxacin 75% resistant and 25% intermediate, to gentamycin 75% sensitive and 25% resistant, and to sulfamethoxazol 100% resistant.
Deteksi Virus Newcastle Disease pada Burung Merpati (Columba Livia) dan Burung Tekukur (Streptopilia Chinensis) yang Menunjukkan Gejala Syaraf Nyoman Reishita Andriyani; Arfian Rahma Shanti; Liza Angeliya; Marla Anggita; Tri Untari; Agnesia Endang Tri Hastuti Wahyuni; Widya Asmara; Michael Haryadi Wibowo
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.220-227

Abstract

Dewasa ini dilaporkan banyak kasus pada burung merpati (Columba Livia) dan burung tekukur (Streptopilia Chinensis) menunjukkan gejala syaraf, terutama: tortikolis, dan kepala gemetar, yang merupakan indikasi penyakit Newcastle Disease (ND). Kedua spesies burung tersebut banyak berkeliaran di lokasi farm ayam komersial untuk mencari pakan. Kondisi tersebut dapat bertindak sebagai faktor penular penyakit ND. Penelitian ini bertujuan mendeteksi virus ND pada burung merpati dan burung tekukur yang memperlihatkan gejala saraf, dengan uji serologis dan molekuler. Sampel darah diambil dari vena brakhialis, diproses untuk mendapatkan serum darah. Serum tersebut selanjutnya diuji dengan uji hemaglutinasi inhibisi (HI), untuk mendeteksi titer antibodi ND. Sampel pool otak, trakea, dan lien diekstraksi RNA-nya dan diamplifikasi dengan primer spesifik gen F virus ND. Sampel pool tersebut juga dikultur pada telur ayam berembrio specific pathogen free (TAB-SPF). Identifikasi dilakukan dengan uji hemaglutinasi (HA) dan HI dengan serum kontrol positif ND. Hasil deteksi serologis 7 sampel burung merpati menunjukkan titer antibodi ND bervariasi dari titer 25 sampai 28., sedangkan 2 sampel diperoleh seronegatif dengan titer 20. Salah satu pool gerusan organ kode M3/Sleman/2021 menunjukkan hasil RT-PCR positif. Analisis sekuens isolat virus ND tersebut termasuk NDV virulen dan dikelompokkan ke dalam genotipe VII-i. Pasase pool sampel organ tersebut masing-masing dikultur pada TAB-SPF dengan pasase sebanyak 3 kali, menunjukkan hasil negatif.
Uji In Vitro Efektivitas Ekstrak Biji Jintan Hitam (Nigella Sativa L.) terhadap Pertumbuhan Microsporum gypseum Penyebab Dermatitis pada Anjing Pasha Glabella; Salma Ramandhanti Putri; Ery Haryani; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69251

Abstract

Biji jintan hitam (Nigella sativa L.) mengandung senyawa aktif seperti thymoquinone, carvacrol, dan thymol yang berperan sebagai antijamur. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Microsporum gypseum yang merupakan salah satu agen penyebab dermatitis pada anjing. Kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Mircrosporum gypseum diuji dengan metode difusi agar dengan teknik sumuran (Agar Well Diffusion). Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak biji jintan hitam terhadap pertumbuhan jamur Microsporum gypseum yaitu 10%, 25%, 50%, 75%, 100%, flukonazol digunakan sebagai kontrol positif dan phospat buffer saline (PBS) kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak biji jintan hitam dengan konsentrasi mulai dari 25%, 50%, 75% dan 100% dapat menghambat pertumbuhan jamur dengan rerata lebar zona hambat secara berurutan ± 3,67 mm., ± 2,84 mm., ± 3,67 mm., dan ± 4,00 mm. Ekstrak biji jintan hitam mampu menghambat pertumbuhan Microsporum gypseum.  Perlu penelitian lebih lanjut untuk mendapatkan hasil optimal sehingga berpeluang sebagai anti fungal pada penyakit dermatitis pada anjing.
Co-Authors Adrenalin, Sruti Listra Agustina Dwi Wijayanti Anak Agung Gede Sugianthara Arfian Rahma Shanti Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabu Dito Anggoro Ery Haryani Fauziah, Ima Herawati, Okti Hilda Susiyanti Debora Berek I wayan Teguh Wibawan Ima Fauziah Ima Fauziah Imanjati, Lynda Nugrahaning Liza Angeliya Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Maria Anggita Marla Anggita Marlin Cindy Claudya Malelak Marseni Banne Ringgi Maxs Urias Ebenheizer Sanam Michael Haryadi Wibowo Nursyirwani, Nursyirwani Nyoman Reishita Andriyani Pasha Glabella Radhian Fadiar Rahmat Setya Adji Rahmat Setya Adji Salma Ramandhanti Putri Santoso, Ferdinand Prayogo Cahyo Setiawan, Dwi Cahyo Budi Setyawan Budiharta Sidna Artanto Sidna Artanto Sidna Artanto Sitarina Widyarini Sitarina Widyarini Sruti Listra Adrenalin Sruti Listra Adrenalin Surya Amanu Surya Amanu Tangkonda, Elisabet Tati Aryani Tri Untari Tri Untari Triyanto . Vinsa Cantya Prakasita Widagdo Sri Nugroho Widagdo Sri Nugroho Widodo Suwito Widodo Suwito Widodo Suwito Widya Asmara Widya Asmara Yovita Devina Yovita Devina