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Rini Sulistiani
Prodi Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Sumatera Utara
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Respons Perkecambahan dan Pertumbuhan Bibit Tomat (Solanum lycopersicum) dalam Beberapa Media Kultur In Vitro Riansyah, M. Afdal; Sulistiani, Rini; Hisham, Nazrul
AGRIUM: Jurnal Ilmu Pertanian Vol 29, No 1 (2026)
Publisher : UNIVERSITAS MUHAMMADIYAH SUMATERA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30596/agrium.v29i1.29097

Abstract

Tomatoes (Solanum lycopersicum L.) are an important horticultural crop with steadily increasing demand in Indonesia, yet their productivity often fluctuates. The availability of high-quality seeds, including those produced through in vitro propagation techniques, is a key factor in producing vigorous and uniform seedlings. The study employed a factorial Complete Randomized Design with two treatments. The aimed to compare the germination and early growth responses of two tomato varieties, MicroTom and MT3, on six different culture media: Full-MS, Half-MS, Full-MS+GA₃ 0.5 mg/L, Chu’s N6, Chu’s N6+GA₃ 0.5 mg/L, and Gamborg B5. The results showed that the culture medium had a significant effect on all parameters, while the variety had a significant effect only on the average germination time and shoot length. The Half-MS, Chu’s N6, and Gamborg B5 media yielded the highest germination rates (70–72%), while Full-MS produced the lowest rate (35%). MT3 exhibited a faster germination rate (6.91 days) and longer shoot length (34.41 mm) compared to MicroTom. The longest root length was observed in the MT3 grown on Half-MS (C2M2), and the longest shoot length was observed in the MT3 grown on Gamborg B5 (C2M6). GA₃ supplementation did not always enhance vigor or early growth. The interaction between variety and medium had a significant effect, particularly on root and shoot growth. This indicates that the effectiveness of the medium depends on the genotype used. Overall, Half-MS, Chu’s N6, and Gamborg B5 are recommended as optimal media for tomato germination and early growth, particularly for the MT3.
Co-Authors Aisar Novita Asritanarni Munar Bayu Bonar Pratama Pohan Bunga Raya Ketaren Cemda, Abdul Rahman Dafni Mawar Tarigan Dini Mufriah Dini Mufriah Dini Mufriah Dini Mufriah Dini Mufriah Fadillah Rasyidi, Ahmad Hakim, Juanda Haron, Farah Farhanah Hasibuan, Etti Suriani Hilda Julia Hilda Julia Hilda Julia Hisham, Nazrul Iqmal bin Jalani, Syazrul Ketaren, Bunga Raya Khulidin, Khairul Asfamawi Lita Nasution Lubis, Efrida Mhd Yusuf Dibisono Muhammad Yusuf Dibisono Nasution, Lita Noviantika Nasution Othman, Abu Bakar Priatmojo, Tito Rasyidi, Ahmad Fadhillah Raya Ketaren, Bunga Riansyah, M. Afdal Rini Susanti Rony Alparizi Sasmita Siregar Sri Utami Surianto Surianto Syaiful Amri Saragih Syarifa Mayly Syarifah Mayly Triyanti, Vitri Renny umami, Wahyuni Wan Arfiani Barus Widihastuty Widihastuty Wizni Fadhillah Yusuf, Mukhtar