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All Journal Majalah Kedokteran Bandung Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Althea Medical Journal Folia Medica Indonesiana Global Medical and Health Communication Medical Laboratory Technology Journal JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Jurnal Pengabdian Pada Masyarakat Majalah Patologi Indonesia Jurnal Neuroanestesi Indonesia JKKI : Jurnal Kedokteran dan Kesehatan Indonesia The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Eduvest - Journal of Universal Studies Media Dermato-Venereologica Indonesiana
Hermin Aminah Usman
Universitas Padjadjaran
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : Medical Laboratory Technology Journal

 1 
Effectiveness of Xylene and Mineral Oil in DNA Extraction from Formalin-fixed Paraffin-embedded Diffuse Astrocytic Tumor Edward Jaya Hadi; Hasrayati Agustina; Hermin Aminah Usman
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.517 KB) | DOI: 10.31964/mltj.v0i0.447

Abstract

Formalin-fixed paraffin-embedded (FFPE) tissue is an important source of material for molecular analysis. In the anatomical pathology field, molecular testing is needed in certain tumors, such as astrocytic tumors, to confirm the diagnosis. Extracting DNA from FFPE material is still challenging. The first important step in the extraction process is deparaffinization. This study aims to compare two types of deparaffinization methods. The first method used xylene, and the second one used mineral oil. The results of this study can be used to develop a reliable protocol for DNA extraction from FFPE tissue. DNA from 28 FFPE diffuse astrocytic tumor tissue blocks was extracted. The quantity and A260/A280 ratio of the DNA was measured by spectrophotometer. PCR assays were performed to assess the suitability of extracted DNA for molecular analysis. The results showed that the xylene group has significantly higher DNA concentrations than the mineral oil group (p<0.01). In both groups, average and median A260/A280 scores were between 1.8-2.0. In PCR assay, both groups show a similar result (27 of 28 samples were successfully amplified). In conclusion, xylene and mineral oil can be used effectively as deparaffinization agents. Both methods generated good quality extracted DNA. The advantage of mineral oil is that it is non-toxic and has shorter hands-on time. Xylene is preferable for a small-sized sample because it produces a higher DNA yield.
Co-Authors . Afiati, . Abdul Hadi Hassan, Abdul Hadi Afiati Afiati Afiati Afiati Ahmad Faried Anglita Yantisetiasti Anjani, Gebby Reviana Apriliani, Ardhia Rizki Arif, Izhar Muhammad Arif, Izhar Muhammad Arima Kurnia Sari Dewi Nurcahyani Astrid Feinisa Khairani Atika Hana Ilyasa Bashari, Muhammad Hasan Bethy Surjawathy Hernowo Bethy Surjawathy Hernowo Bethy Suryawathy Hernowo Billy Parulian Lubis Defi Ardia Pramesti Dewayani, Birgitta Maria Dewi Yulianti Bisri Dika Widia Nur Azizah Dimas Erlangga Luftimas Dimas Erlangga Luftimas Dolvy Girawan Dwi Andini Dwi Putri, Riska Edward Jaya Hadi Egie Rahmalaelani Eka Surya Nugraha Erida, Yulie Fauzan Ali Zainal Abidin Gian Nugraha Gurwinder Kaur a/p Gurcharan Singh Gurwinder Kaur a/p Gurcharan Singh Hasrayanti Agustina Hasrayati Agustina HENDRA GUNAWAN B11211055 Hendrikus Bolly Herry Yulianti Jessica Oktavianus Trisaputra Jono Hadi Agusni Jono Hadi Agusni, Jono Hadi Khafifah, Dinda Salsa Labibah, Salma Nur Lala Yuliani Laraswita, Sarah Meinarni, Retno Misuari Sabirin, Muhammad Syah Muhammad Begawan Bestari Nadiah, Zahra Nadifah Diana Zalia Naura Mardhiyah Nenny Agustanti Nita Novita Noor Fitriana, Maya Novi Vicahyani Utami Nugraha, Marwan Agung Nurhasanah Nurhasanah Nurhidayat, Ade Apon Putri Halleyana Rahmalaelani, Egie Rahman, Putri Halleyana A Ramdhani, Muhammad Farid Rani Septriana Rani Septrina Rania Azzahra Salsazayasya Parikesit Rasdianto, Devi Novianto Reti Hindritiani Rianty, Fathia Rika Mustika Risqi Dwi Putra Sholihah, Fitria Siska Dwiyantie Wahyuni Siti Aminah Abdurachman Sri Suryanti Sunardi, Mas Adi Titing Nurhayati Trisaputra, Jessica Oktavianus Yudi Wahyudi Yunnica Yunnica Zahra Nurusshofa