Claim Missing Document
Check
Articles

Found 23 Documents
Search

Analysis of Exopolysaccharides in Lactobacillus casei group Probiotics from Human Breast Milk Nur Kusmiyati; Yuni Puspitasari; Ulfah Utami; Anggeria Oktavisa Denta
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.2872

Abstract

Exopolysaccharides get a lot of attention because they can improve the host immune system. Exopolysaccharide is a polysaccharide that is produced and secreted from microbes outside the cell, usually found on the outside of the bacterial structure. The Lactobacillus casei group from human breast milk is thought to have the ability to produce exopolysaccharides. The purpose of the study was to examine the exopolysaccharide of the L. casei group that was isolated from breast milk. The methods used include the gravimetric, the phenol-sulfuric acid and the Fourier Transform Infra-Red (FTIR). The results showed that the L. casei group could produce exopolysaccharides, and had high exopolysaccharide total sugar content. Lactobacillus paracasei had the highest exopolysaccharide and total sugar content of 3660 mg/L and 80.6%, respectively. The FTIR results of the L. casei group exopolysaccharides showed the presence of hydroxyl functional groups O-H (3425.76-3295.98 cm-1), methyl C-H (2930.86-2856.70 cm-1), carbonyl C=O (1660.11-1647.27 cm-1), C-H (1456.16-1373.44 cm-1) and C-O-C ether (1071.08-1056.82 cm-1) which are specific characters of exopolysaccharides. Since the FTIR profile demonstrates that the L. casei group can produce exopolysaccharides, it has greater potential as a a probiotic.
Evaluasi Pelaksanaan Program Usaha Kesehatan Gigi Sekolah (UKGS) Di SDN 212 Harapan Kota Bandung Ahdiansyah, Balqis Shafa'; Fatikhah, Nurul; Praptiwi, Yenni Hendriani; Utami, Ulfah
Jurnal Terapi Gigi dan Mulut Vol 3 No 2 (2024): Jurnal Terapi Gigi dan Mulut
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jtgm.v3i2.2120

Abstract

The School Dental Health Program (UKGS) at SDN 212 Harapan in Bandung aims to maintain students' dental health through promotive, curative, and preventive activities but was halted in 2019 due to the COVID-19 pandemic. This study evaluates the program's implementation at SDN Harapan 212 using a descriptive, qualitative approach with in-depth interviews, observations, and documentation. Data were analyzed through transcription, reduction, presentation, and verification. Findings reveal deficiencies in input, such as a lack of human resources (dentists and little dentist) and suboptimal facilities (non-functioning dental units). The organizational structure of UKS/UKGS is not displayed, and there is no parental financial support. In the process, the health center's UKGS program has been halted since 2019, and curative programs are not implemented. Policies like health center SOPs and cooperation MOUs are outdated. However, the program has increased students' understanding of dental care and their independence in maintaining dental hygiene. The study concludes that the UKGS program's implementation is inadequate in terms of input and process. It provides valuable insights for improving the UKGS program in other schools and contributes to the literature on school dental health programs.
Pengaruh Penyuluhan Menggunakan Video Interaktif Terhadap Pengetahuan Menyikat Gigi Dengan Teknik Roll Pada Siswa SDN Sukalangu 3 Kabupaten Pandeglang Husna, Naila Fatihatul; Widyastuti , Tri; Heriyanto, Yonan; Utami, Ulfah
Jurnal Terapi Gigi dan Mulut Vol 3 No 2 (2024): Jurnal Terapi Gigi dan Mulut
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jtgm.v3i2.2155

Abstract

The first step in preventing oral and dental disease is to maintain oral hygiene, by brushing your teeth. This behavior must be taught from an early age for the realization of a better health status. Objective: To determine the effect of counseling using interactive videos on knowledge of brushing teeth with the roll technique in elementary school students. The type of research conducted was analytic, with a pre experiment method with a one group pretest-posttest design. sampling using total sampling of 74 students. The variables in the study were Interactive Video and elementary school students' knowledge. Interactive Video has been tested and deemed feasible by media and material experts. Hypothesis testing using the Wilcoxon sign rank test. The average score of tooth brushing knowledge with the roll technique before counseling in classes I, II, III was 41.25, after counseling it was 68.25. In class IV,V,VI before counseling was 42.35, after counseling became 70.59. Wilcoxon Sign Rank Test analysis shows p-value (0.000) <0.05, there is a significant influence between before and after counseling using interactive videos on tooth brushing knowledge.The results of this study indicate that interactive videos can increase tooth brushing knowledge to elementary school students. So that students will better maintain the cleanliness of their teeth. In conclusion, counseling using animated interactive video media can affect the knowledge of brushing teeth with the Roll technique in students.
Analisis Pertumbuhan Khamir Endofit Buah Salak (Salacca edulis Reinw.) dengan Penambahan Zinc Nur Kusmiyati; Ulfah Utami; Muhammad Riefki Pratama; Liliek Harianie
Al-Kauniyah: Jurnal Biologi Vol 17, No 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.31921

Abstract

AbstrakKhamir endofit buah salak (Salacca edulis Reinw.) dengan kode YIS-3, YIS-4, dan YIS-7 dapat dimanfaatkan untuk fermentasi adonan roti. Kemampuan tersebut dapat ditingkatkan dengan penambahan sumber zinc pada media pertumbuhannya. Tujuan penelitian ini untuk menganalisis pertumbuhan khamir endofit buah salak dengan penambahan zinc dan kualitas roti hasil fermentasi. Metode yang digunakan meliputi peremajaan isolat khamir, penambahan nutrisi pada media pertumbuhan, analisis biomassa khamir, jumlah sel, volume adonan, dan organoleptik. Hasil penelitian pada parameter pertumbuhan menunjukkan bahwa perlakuan dengan penambahan zinc 0,1 g/L menghasilkan biomassa dan jumlah sel lebih tinggi dibandingkan perlakuan kontrol. Biomassa tertinggi dihasilkan oleh YIS-4 yakni 4,13 g/300 mL, sedangkan jumlah sel tertinggi dihasilkan oleh YIS-3 yakni 27,84 x 106 sel/mL. Pada persentase pengembangan menunjukkan bahwa isolat khamir dengan perlakuan zinc 0,1 g/L membutuhkan waktu lebih lama untuk mencapai pengembangan tertinggi, namun volume roti setelah pemanggangan menunjukkan hasil yang lebih baik dibanding perlakuan kontrol. Isolat YIS-4 dengan perlakuan zinc 0,1 g/L menghasilkan volume roti tertinggi (949,54 cm3). Berdasarkan analisis organoleptik dengan parameter warna, aroma, rasa dan tekstur menunjukkan bahwa semua panelis lebih menyukai roti hasil fermentasi isolat YIS-4 dengan perlakuan zinc 0,1 g/L.AbstractThe endophytic yeast of salak fruit (Salacca edulis Reinw.) with codes YIS-3, YIS-4, and YIS-7 can be used for bread dough fermentation. This ability can be increased by adding a zinc source to the growth medium. The purpose of this study was to analyze the growth of the endophytic yeast of salak fruit with the addition of zinc and the quality of fermented bread. The methods used include rejuvenation of yeast isolates, addition of nutrients to growth media, analysis of yeast biomass, cell count, dough volume and organoleptic. The results of research on growth parameters showed that treatment with the addition of zinc 0.1 g/L resulted in higher biomass and cell count than the control treatment. The highest biomass was produced by YIS-4 which was 4.13 g/300 mL, while the highest number of cells was produced by YIS-3 which was 27.84 x 106 cells/mL. The swelling percentage showed that yeast isolates treated with 0.1 g/L zinc took longer to reach the highest swelling, but the volume of bread after baking showed better results than the control treatment. YIS-4 isolate treated with 0.1 g/L zinc produced the highest bread volume (949.54 cm3). Based on organoleptic analysis with parameters of color, aroma, taste and texture, it showed that all panelists preferred bread fermented YIS-4 isolate with 0.1 g/L zinc treatment.
Comparison of chlorine dioxide and chlorhexidine 2% antiseptic in reducing bacterial colony counts as an alternative to DUWLs cleaning: a quasi-experimental study Elfiyatinnufus, Rifqiyah; Mulyanti, Sri; Utami, Ulfah; Malinda, Yuti; Laut, Deru Marah
Padjadjaran Journal of Dentistry Vol 35, No 3 (2023): November 2023
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no3.48357

Abstract

ABSTRACTIntroduction: The inner surfaces of dental unit waterlines (DUWLs) possess an accumulation of any bacteria. Microorganisms can enter dental unit waterlines from water reservoirs. Antiseptics are substances that inhibit the growth of bacteria. Chlorine dioxide is effective in decontaminating microbes in the DUWLs and has a beneficial effect on reducing nosocomial infections. Chlorhexidine effectively prevents the growth of Streptococcus bacteria. The addition of antiseptic agents to the water source contributed to a significant reduction of the cultivable microbial counts in the aerosol. The purpose of this study was to analyze the difference between Chlorine Dioxide 0,1% and Chlorhexidine 2% antiseptics on the number of bacterial colonies in the Dental Unit Waterline. Methods: This study was a quasi-experimental study with a total sample size of 8 dental units that have water tanks in the oral surgery clinic. The sampling technique was total sampling, where the sample was divided into 2 groups, group I using Chlorine Dioxide and group II using 2% Chlorhexidine calculation of colony counts unit using the plate count method. Results: The difference in the number of colonies before being given Chlorine Dioxide was 13,153 CFU/mL and after being given Chlorine Dioxide antiseptic was 6,070 CFU/mL, while before being given 2% Chlorhexidine antiseptic was 12,917 CFU/mL and after being given 2% Chlorhexidine antiseptic was 2,823 CFU/mL. There is a significant difference in the number of bacterial colonies before and after being given Chlorine Dioxide and Chlorhexidine 2% antiseptic with ρ=0.001; Conclusion:  Chlorhexidine 2% reduces bacterial colony forming unit in DUWLs much more than using Chlorine dioxide for the alternative of DUWLs Cleaning Agents.Keywords: DUWLs, chlorine dioxide, chlorhexidine 2%, bacterial colony counts 
Pengaruh Penggunaan Media Kartu Bergambar Terhadap Peningkatan Pengetahuan Kesehatan Gigi dan Mulut Pada Siswa Kelas V SD Negeri 212 Harapan Kota Bandung Arifin, Naufal Aqila; Chaerudin, Denden Ridwan; Utami, Ulfah
Jurnal Terapi Gigi dan Mulut Vol 4 No 1 (2024): Jurnal Terapi Gigi dan Mulut
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jtgm.v4i1.2146

Abstract

Increasing dental health knowledge is very important from an early age because it will have an impact on the health of the teeth and mouth. The way to increase knowledge is by learning. Learning can be done using effective media. The aim of this research is to find out how the use of picture card media influences students' increased understanding of dental and oral health. Pre-experimental research using a single group design before and after the test is the type of research that is needed. Sampling was carried out using total sampling technique. The sample in this study amounted to 53 students. The variables in the research were picture card media and student knowledge. The picture card media has been tested and deemed appropriate by media and materials experts. Hypothesis testing uses the Wilcoxon test. The average score for oral health knowledge before the intervention was 70.96 and after the intervention it increased to 96.12. Wilcoxon Sign Rank Test analysis shows p-value (0.000)
ASUHAN KESEHATAN GIGI DAN MULUT IBU HAMIL PADA NY MA DENGAN KASUS GINGIVITIS Azizah, Dita Nur; Utami, Ulfah; Nurjanah, Neneng; Marah Laut, Deru
Jurnal Terapi Gigi dan Mulut Vol 4 No 1 (2024): Jurnal Terapi Gigi dan Mulut
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jtgm.v4i1.2745

Abstract

Pregnancy is a common event in a woman's life that involves physiological and hormonal changes. The risk of dental disease increases during pregnancy, due to several factors such as an increased gag reflex and behavioral changes such as poor oral hygiene that can increase the risk of tooth decay and periodontal disease. Gingivitis is a periodontal disease characterized by red gums and is caused by plaque and other factors including hormonal factors that often occur during pregnancy. The purpose of this study was to determine the results of oral health care in pregnant women. This research method is a case report with the concept of oral health care which consists of the process of assessment, diagnosis, planning, implementation and evaluation. After the examination, the diagnosis was stated that there were 5 basic human needs that were not met. Implementation carried out on clients is cleaning tartar, ART filling, counseling with the Chair Side Talk method, oral physiotherapy and referral to the dentist for further treatment. The evaluation results show that there are 3 basic human needs that have been achieved and 2 basic human needs that have only been partially fulfilled, so the 8 basic human needs of clients have not been fully met.
RISIKO PENULARAN PENYAKIT TUBERKULOSIS KEPADA KELUARGA KONTAK SERUMAH MELALUI ASPEK KESEHATAN GIGI DI WILAYAH KERJA PUSKESMAS PADASUKA KOTA BANDUNG Fitriyani, Annisa Fuzzi; Laela, Dewi Sodja; Utami, Ulfah
Jurnal Terapi Gigi dan Mulut Vol 4 No 1 (2024): Jurnal Terapi Gigi dan Mulut
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jtgm.v4i1.2758

Abstract

Pulmonary tuberculosis (TB) transmission can occur indoors via airborne droplets containing TB bacteria. Household contacts are at increased risk of infection. Sharing toothbrushes or storing them in close proximity can lead to cross-infection. This study aimed to identify risk factors for TB transmission among close household contacts through dental hygiene practices. A qualitative descriptive approach was employed, utilizing in-depth face-to-face interviews and observations. Three BTA- positive patients were recruited as subjects. The study variable was risk factors for TB transmission to close household contacts through dental hygiene practices. Findings revealed that BTA-positive patients had a habit of storing toothbrushes together with family members and had close contact with positive household members. Storing toothbrushes together with bristles touching can facilitate cross- infection. Sharing and storing toothbrushes in close proximity increases the risk of TB cross-infection. Proper toothbrush storage and usage, particularly among TB patients, are crucial to prevent cross-infection via contaminated bristles.
Hubungan tingkat pengetahuan tentang pengendalian infeksi silang terhadap kepatuhan pelaksanaan pada peserta didik di RSGM Universitas Jenderal Achmad Yani: studi cross sectional Kamila, Syadza Afifah; Mulyanti, Sri; Utami, Ulfah; Nurnaningsih, Hera
Padjadjaran Journal of Dental Researchers and Students Vol 8, No 3 (2024): October 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v8i3.58132

Abstract

ABSTRAKPendahuluan: Tenaga kesehatan gigi memiliki risiko tinggi dalam penularan infeksi karena berkontak dengan saliva dan darah saat perawatan gigi. Survei awal menunjukkan masih banyak peserta didik belum menerapkan pengendalian infeksi silang padahal hal ini dapat meningkatkan risiko terpapar mikroorganisme penyebab infeksi. Tujuan penelitian ini untuk menganalisis hubungan tingkat pengetahuan tentang pengendalian infeksi silang terhadap kepatuhan pelaksanaannya pada peserta didik di RSGM UNJANI. Metode: Jenis penelitian ini yaitu penelitian survei analitik dengan rancangan penelitian cross-sectional dengan sampel 60 orang peserta didik di RSGM UNJANI. Pengumpulan data menggunakan kuesioner dan observasi. Hasil: Penelitian menunjukkan tingkat pengetahuan peserta didik dengan kategori baik 23,3%, kategori cukup 66,7%, dan kategori kurang 10%. Tingkat kepatuhan didapatkan hasil 80% kategori patuh dan 20% kategori tidak patuh. Uji statistik Kendall’s-Tau menunjukkan hasil nilai p = 0,047 (p < 0,05). Hubungan kedua variabel bersifat satu arah, artinya semakin baik pengetahuan maka semakin baik pula kepatuhan terhadap pengendalian infeksi silang. Simpulan: berdasarkan temuan uji statistik Kendall's Tau, terdapat hubungan satu arah antara tingkat pengetahuan mahasiswa RSGM UNJANI mengenai pengendalian infeksi silang dengan kepatuhan pelaksanaannya, artinya semakin baik tingkat pengetahuan peserta didik mengenai pengendalian infeksi silang, maka semakin tinggi pula tingkat kepatuhan mereka dalam menerapkan langkah-langkah pengendalian infeksi silang tersebut.KATA KUNCI: Pengetahuan, kepatuhan, pengendalian infeksi silang.The relationship between the level of knowledge about cross-infection control and its compliance among students at Jenderal Achmad Yani University Dental Hospital: cross sectional study ABSTRACTIntroduction: Due to their frequent contact with blood and saliva while providing dental care, dental healthcare personnel are particularly vulnerable to infection transmission. According to a preliminary study, a large number of students have not yet put cross-infection control procedures into place, even though doing so may raise their risk of coming into contact with pathogens. The purpose of this research is to ascertain how students at the RSGM UNJANI relate to their level of cross-infection control knowledge and their compliance with its implementation. Methods: This type of research is analytical research with a cross-sectional research design with a sample of 60 students at UNJANI RSGM. Data collection using questionnaires and observation. Results: The results of the survey indicate that the students' knowledge levels fall into three categories: "good" at 23.3%, "sufficient" at 66.7%, and "poor" at 10%. According to the level of compliance, 20% are not in conformity, while 80% are. Using the statistical test of Kendall's Tau, the p-value was 0.047 (p < 0.05). The two variables have a unidirectional association, which means that higher knowledge corresponds to higher compliance with cross-infection control methods. Conclusion: The results of the Kendall's Tau statistical test indicate that there is a unidirectional association between students' compliance with the implementation of cross-infection control and their understanding of it. This means that the better the students' knowledge of cross-infection control, the higher their compliance in applying the infection control measures.KEY WORDS: Knowledge, compliance, cross-infection control
DAYA HAMBAT DARI EKSTRAK SARANG BURUNG WALET PADA PERTUMBUHAN BAKTERI STREPTOCOCCUS MUTANS DAN PORPHYROMONAS GINGIVALIS Utami, Ulfah; Putri, Megananda Hiranya; Nurjanah, Neneng; Eldarita, Eldarita
Media Penelitian dan Pengembangan Kesehatan Vol. 34 No. 1 (2024): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v34i1.1790

Abstract

Type 2 diabetes (T2DM) is one of the fastest growing global diseases of the 21st century, with its prevalence more than tripling in the last 20 years and affecting 10.5% of the world's population aged 20-79 years. In Indonesia, diabetes mellitus among 20-79 year olds is 10.6%. The most widely cultivated type of swallow nest is Collocatia fuciphaga. Collocatia fuciphaga is a species of bird that produces nests from its white saliva. Swallow's nest contains sialic acid which is only produced from saliva, calcium, glycoproteins and Epidermal Growth Factor (EGF), one of the benefits of which is to accelerate wound healing. The study aimed to determine the inhibition of swallow's nest extract on the growth of Streptococcus mutans and Porphyromonas gingivalis bacteria. The swallow's nest extract test used an experimental design approach, namely conducting laboratory tests. The initial stage was to make swallow's nest extract by maceration technique using 96% ethanol solvent (the aim is to get anti-bacterial active substances, namely D-Galactose and Sialic acid contained in swallow's nest), the result of the extraction was a dry extract weighing 1.8195 grams which was then dissolved again using DMSO solution with concentrations of 10%, 20%, and 30%. The second stage is the swallow's nest extract test with the two test bacteria. The results of the swallow's nest extract test at concentrations of 10%, 20%, 30% against Streptococcus mutans and Porphyromonas gingivalis bacteria showed no inhibition. Future research could be further developed toward wound healing