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All Journal Jurnal Kedokteran Brawijaya Jurnal Kesehatan Andalas Global Medical and Health Communication Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Herb-Medicine Journal Biosaintifika: Journal of Biology & Biology Education PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Molekul: Jurnal Ilmiah Kimia Journal Of Bionursing JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Gunung Djati Conference Series
Nur Signa Aini Gumilas
Universitas Jenderal Soedirman
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The decrease of histomorphometry and function of pancreas in male albino rats after induced by sleep deprivation Fitranto Arjadi; Nur Signa Aini Gumilas; Ika Murti Harini; Pugud Samodro; Andromeda Andromeda
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 12, No 2, (2021)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol12.Iss2.art3

Abstract

Background: Sleep disturbance accelerates type 2 diabetes mellitus by reducing insulin secretion and by the occurrence of difficulty in controlling sleep behaviour in humans so that it is necessary conduct a study by using experimental animals.Objective: This study aims to determine histophotometric differences of pancreas and differences of glucose tolerance in male albino rats (Rattus norvegicus) after induced by stress paradoxical sleep deprivation (PSD) and total sleep deprivation (TSD). Methods: This study applied a post-test only with control groups consisting of 30 male albino rats (Wistar strain) divided randomly into 3 groups: control group, PSD group and TSD group. The mean number and diameter of islets of Langerhans were calculated per slide and were averaged for each group at a magnification of 400 X. The percentage of beta cells in one islet of Langerhans was (ß-p) that was a number of normal beta cells (Bn) divided for the total beta cells (Bt) (normal and damaged) x 100%. The glucose level based on the sampling time was made a curve shape, and the area under the 0-to-120-minute curves (AUC0-120) was calculated by using a trapezoidal formula.Results: The lowest AUC0-120 level of glucose was the control group (14,082 ± 955mg/dL) and the highest was the TSD group (16,293 ± 2,195 mg/dL); the Post-Hoc LSD test showed a significant difference (p<0.05) between the control-PSD group and the TSD-control group. The mean result of pancreatic ß cells mass with the lowest number was in the TSD group (76.3 ± 4.8), and the highest result was in the control group (94.3 ± 2.7); Post-Hoc LSD showed a significant difference (p <0.05) in the control-PSD group and the TSD-control group. The lowest mean in cell diameter of islets of Langerhans was in the TSD group (0.132 ± 0.031 mm), and the highest mean was in the control group (0.213 ± 0.019 mm). Conclusion: Sleep deprivation could reduce pancreatic ß cell mass and cell diameter of islets of Langerhans and increase glucose tolerance levels.
Faktor-Faktor yang Mempengaruhi Tingkat Kepatuhan Diet pada Pasien Diabetes Melitus Tipe 2 di Kecamatan Sumbang Banyumas Dwi Arini Ernawati; Ika Murti Harini; Nur Signa Aini Gumilas
Journal of Bionursing Vol 2 No 1 (2020): Journal Of Bionursing
Publisher : Fakultas Ilmu-ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.145 KB) | DOI: 10.20884/1.bion.2020.2.1.40

Abstract

Background problem: Diabetes Mellitus (DM) prevalence in Indonesia based on the results of basic health Research (RISKESDAS) year 2013 is 2.1%, in Central Java  18.33%. Banyumas District has an incidence rate of 1,417 in 2014 and Sumbang Sub-district has the highest incidence rate of DM type 2. Management in DM sufferers is integrated, including eating arrangements, physical activity, education, and medicines. Meal arrangement for patients with type 2 DM includes setting against 3J: number, type and meal schedule in a day. Patients with DM who have already run a diet program has not been able to control blood glucose properly. Food arrangement often fails due to the disobedience of sufferers in running their diet. Many factors affect dietary compliance, among them the level of education, level of knowledge, self-motivation, family support and the role of health workers.Objective: To determine the factors that affect the compliance of diet in diabetes mellitus type 2 patients in Sumbang Sub-district of Banyumas.Methods: The studies used are cross sectional, the level of compliance seen from 3J, i.e. precise amounts, precise types and precise schedules. Samples taken from DM patients who are in Prolanis present during laboratory examination in June 2019. Sampling technique using total sampling. Bound variables are the level of dietary compliance, the free variables are education levels, knowledge levels, self-motivation, family support and the role of health workers.Results: The number of samples was 32 people, who were obedient in a diet of 15 people (46.9%), who did not obey 17 people (53.1%). The P value of the factor variable factors affecting the diet is: age p = 0.411, sex p = 0.035, Job p = 0131, long DM p = 0.261, education level p = 0.035, knowledge level p = 0.036, self motivation p = 0.464, family support p = 0.314, and P Health worker, p = 0.452. Variables that have a meaningful relationship (P < 0.05) with dietary compliance levels are gender, level of education and level of knowledge. As for the variable age, employment, old DM, self-motivation, family support, and the role of health workers have an insignificant relationship (P > 0.05Conclusion: From 32 patients DM most have a level of diet compliance in the category of disobedient 17 people (53.1%), there is a meaningful relationship between gender, education level and level of knowledge with DM dietary compliance,
Combination of Vitamin C and E Improves Spermatogenesis of White Male Rat Model of Paradoxical Sleep Deprivation Stress Fitranto Arjadi; Mustofa Mustofa; Yudhi Wibowo; Nur Signa Aini Gumilas; Dzicky Rifqi Fuadi
Jurnal Kedokteran Brawijaya Vol. 32 No. 1 (2022)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2022.032.01.2

Abstract

Paradoxical Sleep Deprivation (PSD) elevates glucocorticoid and Reactive Oxygen Species (ROS) levels that cause oxidative stress, trigger spermatogenic cells damage, and reduce the number of sertoli cells. Vitamin C and E are antioxidants that could prevent spermatogenesis damage by preventing free radical formation. The study aimed to determine the effect of single and combined doses of vitamin C and E in improving spermatogenesis of white male rats (Rattus norvegicus) model of PSD. This is experimental research with post-test only and control group design on 28 white male rats distributed into four groups, i.e. group I (control, PSD), group II (PSD+vitamin C), group III (PSD+vitamin E), and group IV (PSD+a combination of vitamin C and E). Testicular preparations were stained using Hematoxylin-Eosin staining, quantitative scores of spermatogenic cells were measured using the Johnsen method, and the number of sertoli cells was counted in 10 seminiferous tubules in each of the three testicular sections. Group I has the lowest mean of Johnsen score (5.27±0.28), and group IV has the highest mean score (8.95±0.62), while the Mann-Whitney test showed a significant difference (p<0.05) between group II, III and IV compared to group I (control group). Mean sertoli cells number of group I is the lowest (10.66±1.04), and group IV has the highest sertoli cells number. The post-hoc LSD test showed a significant difference (p<0.05) between group IV and the other groups. Thus, the combination of vitamins C and E improves Johnsen score and sertoli cells number of male rats (Rattus norvegicus) experiencing PSD.
Perbedaan Jumlah Sel Sertoli Pasca Pemberian Ekstrak Etanol Akar Purwoceng (Pimpinella alpina) ANNISA FARAH FADHILAH; FITRANTO ARJADI; NUR SIGNA AINI GUMILAS
Gunung Djati Conference Series Vol. 15 (2022): Prosiding Seminar Nasional Kimia Tahun 2022
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.688 KB)

Abstract

Paradoxical Sleep Deprivation (PSD) is one form of stress models in laboratory animals resulting in oxidative stress. Oxidative stress can decrease the number of Sertoli cells. Ethanol extract of purwoceng roots containing active substances for proliferation of Sertoli cells is expected to increase the number of Sertoli cells. Objective: To determine differences in the number of Sertoli cells after administration of ethanol extract of purwoceng roots in male rats after stress model of PSD induction. Method: This was an experimental research with posttest only with control group design. Thirty rats were divided into six groups: group A (negative control), group B (PSD with 7 days sleep recovery), group C (PSD), group D (PSD with 16.75 mg/ml/200grBB of ethanol extract of purwoceng roots), group E (PSD with 33.50 mg/ml/200grBB of ethanol extract of purwoceng roots), and group F (PSD with 50.25 mg/ml/200grBB of ethanol extract of purwoceng roots). Induction of PSD conducted for 96 hours and followed by administration of ethanol extract of purwoceng roots for 7 days. Result: The number of Sertoli cells in group A had the highest rates (29,17±3,58), followed by group D (28,77±3,16), group E (27,66±2,51), group B (27,45±1,45), group F (27,44±1,71), and group C (27,29±1,54). Kruskal-Wallis test showed the value of p=0,858. Conclusion: There was no significant difference in the mean number of Sertoli cells of male white rats after administration of various doses of ethanol extract of purwoceng roots.
The Expression of mRNA LMP1 Epstein-Barr Virus from FFPE Tumour Biopsy: a Potential Biomarker of Nasopharyngeal Carcinoma Diagnosis Daniel Joko Wahyono; Nur Signa Aini Gumilas; Hidayat Sulistyo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9028

Abstract

Nasopharyngeal carcinoma (NPC) is a multifactorial disease that is endemic geographically in the world. Indonesian population has a highly incidence rate that is 6.2/100,000 people year. The pathogenesis of NPC is more directly reflected by carcinoma-specific viral transcriptional activity at the site of primary tumour. Epstein-Barr virus (EBV) infection in NPC is reflected by the expression of EBV latent and lytic gene. In fact, mRNA Latent Membrane Protein 1 (LMP1) EBV expression was an important latent infection biomarker. The aim of this study was to determine a potential use of relative expression of mRNA LMP1 EBV from formalin-fixed paraffin embedded (FFPE) tumour biopsy in NPC as a tumour biomarker. This reseach design was a cross sectional study. The samples were the archived specimens of FFPE tumour biopsy from NPC WHO-3 patient which were collected from untreated patients from 2014 in the Department of Pathology Anatomy, Prof. dr. Margono Soekarjo Hospital, Purwokerto. The expression of mRNA LMP1 EBV expression was determined by RT-PCR technique. The positivity of mRNA LMP1 EBV expression was 51.9%, indicating a moderate positivity. The result proved that the expression of mRNA LMP1 EBV from FFPE NPC WHO-3 tumour biopsy was a potential biomarker of NPC diagnosis. The molecular methods would improved the management of NPC, particularly in the histopathological diagnosis of NPC.
Effect of Sleep Deprivation on the Number of Prefrontal Cortex Neuroglia Cells in Male White Rats (Rattus norvegicus) Arjadi, Fitranto; Kuncoro, Prasetyo Tri; Gumilas, Nur Signa Aini; Harini, Ika Murti; Mustofa, Mustofa; Wisesa, Sindhu
Global Medical & Health Communication (GMHC) Vol 11, No 2 (2023)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v11i2.10743

Abstract

Stress induced by sleep deprivation can increase inflammation and oxidative stress, destroying the pyramidal and neuroglia cells in the prefrontal cerebral cortex and interrupting cognitive and behavioral functions. This study aims to observe the difference in the number of pyramidal and neuroglia cells in the prefrontal cortex of male white rats (Rattus norvegicus) after stress induction by paradoxical sleep deprivation (PSD) and total sleep deprivation (TSD). This study was conducted in the Anatomy Laboratory of the Faculty of Medicine, Universitas Jenderal Soedirman, from November 2019 to February 2020. The method of this study was a posttest-only design with a control group approach using ten rats for each group; that was control (K.I.), PSD (KII), and TSD (K.I.). PSD and TSD groups received sleep deprivation treatment for eight days for 20 hours/day and 24 hours/day, respectively. The mean pyramidal cell number decreased in the PSD (66.67±24.55) and TSD (65.90±34.91) compared to the control (77.10±26.11) group, but no significant differences were found between all groups (p>0.05). The mean neuroglial cell number was lower in the PSD (97.78±28.17) and TSD (75.80±22.39) compared to the control (126.00±48.81). Post-hoc Bonferroni test showed a significant difference between control and TSD (p<0.05) but not between control and PSD or PSD and TSD (p>0.05). In conclusion, there was a significant difference in the number of neuroglial cells but not pyramidal cells in the prefrontal cortex of male white rats (Rattus norvegicus) after stress induction with total sleep deprivation (TSD).
Co-Authors Adiningtyas, Pradani Eva Aminuyati Andromeda Andromeda ANNISA FARAH FADHILAH Anton Budi Dharmawan Arin Herkilini Daniel Joko Wahyono Daniel Joko Wahyono Dwi Arini Ernawati Dwi Arini Ernawati Dzicky Rifqi Fuadi Eman Sutrisna Evy Sulistyoningrum Fitranto Arjadi Fitranto Arjadi FITRANTO ARJADI Harini, Ika Murti Hidayat Sulistyo Hidayat Sulistyo Ika Murti Harini Kuncoro, Prasetyo Tri Kusuma, Muhammad Nanang Himawan Lantip Rujito Mohammad Nanang HK, Mohammad Nanang Muhamad Rifqy Setyanto Mus topa Mustofa Mustofa Mustofa Mustofa P Maria Hendrati Pancrasia Maria Hendrati Pugud Samodro Qurrotu Aini Rahmah, Sarah Shafira Aulia Sucipto, Muhammad Cahya Riadi Susiana Candrawati Tri Yulia Ningsih Uli Nurjanah Vitasari Indriani Wisesa, Sindhu Yudhi Wibowo