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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Indonesia Chimica Acta Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Chemistry Indonesian Journal of Chemical Research Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Makara Journal of Science Analit: Analytical and Environmental Chemistry Indonesian Journal of Jamu

Wulan Tri Wahyuni

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia

Author-ID : 411386

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Data Fusion of UV-Vis and FTIR Spectra Combined with Principal Component Analysis for Distinguishing of Andrographis paniculata Extracts Based on Cultivation Ages and Solvent Extraction Antonio Kautsar; Wulan Tri Wahyuni; Utami Dyah Syafitri; Syifa Muflihah; Nursifa Mawadah; Eti Rohaeti; Zulhan Arif; Bambang Prajogo; Muhammad Bachri Amran; Abdul Rohman; Mohamad Rafi
Indonesian Journal of Chemistry Vol 21, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.60321

Andrographis paniculata is one of the medicinal plants used for the treatment of antidiabetic. Cultivation ages and solvent extraction affected metabolites' composition and concentration that directly cause the plant's efficacies. This research aimed to distinguish A. paniculata based on cultivation ages and solvent extraction using data fusion of UV-Vis and FTIR spectra combined with principal component analysis (PCA). A. paniculata with 2, 3, and 4 months post-planting were extracted by water, 50% ethanol, 70% ethanol, and ethanol. In each extract, we measured UV-Vis and FTIR spectra. Then, we used the data fusion from both spectra. We used UV-Vis and FTIR absorbance from 200–400 nm and 1800–400 cm–1, respectively. Each extract gives a similar pattern of UV-Vis and FTIR spectra, only differ in their intensities. PCA score plot in two and three-dimensional showed A. paniculata extracts could be distinguished based on cultivation ages and solvent extraction with a total variance of 86 and 92%, respectively. Furthermore, this study confirms the data fusion of UV-Vis and FTIR spectra could distinguished A. paniculata extracts combined with chemometrics based on cultivation ages and solvent extraction.

Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique Wulan Tri Wahyuni; Rudi Heryanto; Eti Rohaeti; Achmad Fauzi; Budi Riza Putra
Jurnal Kimia Sains dan Aplikasi Vol 24, No 2 (2021): Volume 24 Issue 2 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.

Antioxidant Activity of Daemonorops draco Resin Sri Purwanti; Wulan Tri Wahyuni; Irmanida Batubara
Jurnal Kimia Sains dan Aplikasi Vol 22, No 5 (2019): Volume 22 Issue 5 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

Pembuatan Recycle Screen Printed Carbon Electrode dan Aplikasinya untuk Deteksi Asam Galat dengan Teknik Voltammetri Wulan Tri Wahyuni; Rany Zalvianita; Rudi Heryanto
Jurnal Kimia Sains dan Aplikasi Vol 22, No 5 (2019): Volume 22 Issue 5 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Gallic acid is phenolic compound found in tea and act as antiradical agent. This compound is electrochemically active and could be detected using voltammetric technique. This study aims to obtain recycle screen printed carbon electrode (rSPCE) and applied it for voltammetric detection of gallic acid. rSPCE was prepared by modification of used SPCE working electrode using a mixture of graphite:polystyrene at ratio of 95:5, 90:10, and 85:15 (w/w). Graphite:polystyrene was suspended in chloroform and deposited on SPCE by drop casting method. Phosphate buffer 0.1 M at pH of 2.5, 3.5, and 7 were used as electrolyte solution in voltammetric detection of gallic acid. The optimum condition of gallic acid detection was obtained when phosphate buffer 0,1 M at pH of 2.5 and rSPCE in composition of graphite:polystyrene (95:5) used as electrolyte and working electrode, respectively. Gallic acid has 2 oxidation peaks at potential of 0.26 V and 0.63 V vs Ag/AgCl, respectively. At concentration of 1 – 5 mM, gallic acid and oxidation currents provide linear regression with the coefficient determination of 0.9947 and 0.9864, consecutively for peak number 1 and 2. Measurement of gallic acid at rSPCE 95:5 shows good precision with %RSD < 5%. rSPCE was successfully applied for voltammetric detection of gallic acid in standard solution with accuracy > 96%, however further development is needed before its application for gallic acid measurement in real sample such as tea extract.

Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker Badrunanto Badrunanto; Wulan Tri Wahyuni; Hera Nirwati; Mohamad Rafi
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.

Screening and Optimization Consortium Technique of the Bacillus megatherium and Saccharomyces cerevisiae Microbial Consortium for Ethanol Detection Nurdiani Nurdiani; Dyah Iswantini; Novik Nurhidayat; Wulan Tri Wahyuni; Agus Kartono
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 1, May 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i1.31506

Determination of alcohol content is very important in the food and beverage industry. Biosensor is an alternative to measuring ethanol content. Alcohol biosensors with a single microbe still have a narrow measurement area at ethanol concentrations, so a microbial consortium is needed to widen the range of measured ethanol concentrations. Therefore, it is necessary to screen the microbes from Bacillus sp and S. cerevisiae which have the potential to produce alcohol dehydrogenase (ADH) enzymes and optimize the consortium technique that can provide the best response to oxidation currents. In the yeast microbial screening of 14 S. cerevisiae isolates and 5 Bacillus sp isolates, it can be concluded that for the 14 yeast S. cerevisiae isolates that have the potential to produce ADH enzymes, there is one isolate with the SCRF code. For the 5 bacterial isolates of Bacillus sp that have the potential to produce ADH enzymes, all Bacillus isolates with the code Bacillus megatherium 29/9/14, Bacillus megatherium 23/6/22, Bacillus 6, Bacillus 53, and Bacillus 55. Based on the oxidation current data, Bacillus megatherium 23 /6/22 produces the highest current compared to other Bacillus isolates. The consortium technique that provides the highest current is the method of mixing 1:1 (µL) microbial suspension in an Eppendorf container. Optimization of the consortium's biofilms using the Response Surface Method was produced at 10 days of age, pH 7.5, and 75 µL of microbial suspension dripping volume.

PEMANFAATAN VIRGIN COCONUT OIL SEBAGAI FOAMING AGENT PADA BETON RINGAN Salmi Afifah; Ria Apriana; Aditya Widianto; Wulan Tri Wahyuni
Analit: Analytical and Environmental Chemistry Vol 6, No 2 (2021): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Foaming agent dapat disintesis dengan mereaksikan asam lemak dari minyak nabati seperti asam laurat dari virgin coconut oil (VCO) dengan alkali. Foaming agent dari asam laurat VCO berpotensi sebagai salah satu aternatif pengganti foaming agent sintetik yang sulit terdegradasi. Penelitian ini bertujuan memformulasi foaming agent berbahan dasar VCO untuk menguji efektivitas kinerja foaming agent yang dihasilkan dalam aplikasi pembuatan beton ringan. Pemisahan asam laurat dari VCO menggunakan metode saponifikasi menggunakan NaOH 3,5 N. Formulasi foaming agent (K-laurat) dilakukan dengan mencampurkan asam laurat dengan KOH 30% b/v pada perbandingan 1:0,5. Hasil yang diperoleh menunjukkan rendemen asam laurat dari VCO adalah sebesar 69,1% v/v sementara foaming agent berupa K-laurat yang diperoleh adalah sebanyak 738 g. Foaming agent yang dihasilkan memiliki pH 8,15, densitas 0,9976 g/mL, viskositas 5,43 cP, dan sudut kontak 35,81o. Kinerja foaming agent menunjukkan stabilitas busa sebesar 84,4−87,5%, kemampuan pembusaan sebesar 385−533% selama 45 menit, serta diameter busa sebesar 4,3−64,8 μm. Beton ringan yang dibuat dengan foaming agent dari asam laurat VCO memiliki tekstur sedikit kasar, berpori dan mengeras setelah 1 hari. Kinerja beton ringan yang dihasilkan memiliki densitas 1497,91 kg/m3 dan kuat tekan 2,8033 MPa.http://dx.doi.org/10.23960/aec.v6.i2.2021.p156-167

Electrochemical Detection of Thiamine using Differential Pulse Voltammetry at PEDOT:PSS modified Glassy Carbon Electrode Ulfiatun Nisa; Aninda Naszwa Kuswanto; Nur Zamilah; Budi Riza Putra; Wulan Tri Wahyuni
Analit: Analytical and Environmental Chemistry Vol 7, No 02 (2022): ANALIT: ANALYTICAL AND ENVIRONMENTAL CHEMISTRY
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

The development of easy and rapid detection method for vitamin in food is interesting to study because this compound is an essential nutrient to maintain the metabolism in the human body. Herein, this work aims to detect vitamin B1 (thiamine) content in the supplement of commercial vitamin by differential pulse voltammetry technique using a glassy carbon electrode modified poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT: PSS) and compare the results with the spectrophotometry UV-Vis. The electrochemical investigations were evaluated to a glassy carbon electrode modified PEDOT: PSS in terms of the parameter of analytical performances including linearity, precision, detection limit and quantitation limit. Based on the results, the oxidation peak of thiamine observed at potential of 250 mV vs Ag/AgCl with its current intensity measured 2.5 times higher than detected using unmodified glassy carbon electrode. In addition, the measurement of thiamine in the concentration range of 1 – 10 mM using a glassy carbon modified PEDOT:PSS displayed a good value for several parameters such as linearity (R2= 0.9913), precision (percentage of relative standard deviation as 3.9%), and the detection and quantitation limit as 2.5×10-5 M and 7×10-5 M, respectively. As a comparison in real analysis, this developed method was then used to measure thiamine in the sample of commercial vitamin and showed the accuracy value as 95.86%. This value was then compared with the real value and showed a non-significant different when analysed with t-student test at the confidence interval of 95%.

AKTIVITAS ANTIBAKTERI DAN TOKSISITAS MINYAK SERAI DAPUR (Cymbopogon citratus) DENGAN PERLAKUAN PEMEKATAN PADA SUHU BERBEDA Reni Febriani; Eti Rohaeti; Wulan Tri Wahyuni
Analit: Analytical and Environmental Chemistry Vol 6, No 2 (2021): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Minyak atsiri serai (Cymbopogon citratus) dapur telah dipergunakan secara luas di berbagai industri, diantaranya sebagai agen antibakteri. Pada penelitian ini dievaluasi aktivitas antibakteri dan toksisitas minyak serai dapur yang dipekatkan pada suhu berbeda. Toksisitas dievaluasi melalui uji brine shrimp lethality test (BSLT), sementara uji antibakteri dilakukan terhadap bakteri Staphylococcus aureus dan S. epidermidis dengan metode difusi dan dilusi. Komponen kimia yang terdapat dalam minyak serai dianalisis menggunakan kromatografi gas spektrometri massa (GC-MS). Hasil yang diperoleh menunjukkan berdasarkan uji BSLT diperoleh nilai lethal concentration 50 (LC50) sampel minyak serai dapur semua perlakuan < 1000 ppm dengan LC50 terendah sebesar 16,53 ± 2,82 ppm. Nilai konsentrasi hambat minimun (KHM) dan konsentrasi bunuh minimum (KBM) minyak serai dapur terhadap S. aureus umumnya lebih rendah dibanding terhadap S. epidermidis. Aktivitas antibakteri minyak serai dapur terhadap S. aureus terkategori sangat kuat dengan nilai KHM dan KBM terendah ditunjukkan oleh minyak serai tanpa perlakuan pemekatan yaitu sebesar 1250 dan 2500 ppm terhadap S. aureus. Komposisi senyawa kimia utama dalam minyak serai dapur berdasarkan analisis GC-MS ialah sitral, neral dan β-myrcenehttp://dx.doi.org/10.23960/aec.v6.i2.2021.p168-179

FABRICATION AND ANALITICAL PERFORMANCE EVALUATION OF CARBON PASTE ELECTRODE USING CYCLIC VOLTAMMETRY Wulan Tri Wahyuni; Latifah K Darusman; Desi Herliani
Analit: Analytical and Environmental Chemistry Vol 2, No 2 (2017): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Penelitian ini bertujuan mengembangkan metode pembuatan Elektrode Pasta Karbon (EPK) yang memberikan respon voltammetri yang optimum serta menguji kinerjanya menggunakan larutan K3[Fe(CN)6] secara voltammetri siklik. Parameter yang dioptimasi meliputi nisbah komposisi grafit dan parafin, pemanasan grafit, sonikasi campuran grafit dan parafin, waktu penggerusan, dan lama penyimpanan EPK sebelum digunakan. Respons terbaik diperoleh saat EPK dibuat dengan nisbah campuran grafit dan parafin sebesar 7 : 3 dan grafit yang dipanaskan terlebih dahulu pada suhu 105 C selama 2 jam. Campuran grafit dan parafin terbaik diperoleh setelah sonikasi selama 30 menit dan waktu penggerusan selama 45 menit. EPK yang disimpan 2 hari sebelum digunakan menghasilkan respons terbaik. Pengukuran K3[Fe(CN)6] pada rentang konsentrasi 0,1 – 125 mM menunjukkan respon linear dengan koefisien determinasi (R2) sebesar 0,9998 dan 0,9991, masing-masing untuk arus puncak oksidasi dan reduksi dengan simpangan baku relatif (%SBR) sebesar 2,0% pada reaksi oksidasi dan 1,07% pada reaksi reduksi. EPK yang dihasilkan menunjukkan stabilitas yang baik pada pemakaian selama 7 hari berturut-turut dan 96 kali pengukuran

Co-Authors Abdul Rohman Achmad Fauzi Achmad Fauzi Aditya Widianto Agus Kartono Ahmad, Shahrul Nizam Aji Hamim Wigena Aninda Naszwa Kuswanto Anindya, Weni Antonio Kautsar Apriliani Rahmat Asoka, Shadila Fira Badrunanto Badrunanto Badrunanto, Badrunanto Bambang Prajogo Eko Wardoyo Budi Riza Putra Budi Riza Putra Budi Riza Putra Desi Herliani Dinda Iryawati Dyah Iswantini Era Rahmi Erna Subroto Eti Rohaeti Fauziyatul Munawaroh Fransiska Devy Junardy Hera Nirwati Herdiyanto Herdiyanto Ika Kartika Irma Herawati Suparto Irmanida Batubara Karomah, Alfi Hudatul Kilala Tilaar Latifah K Darusman Latifah K Darusman Latifah K Darusman Pitria Latifah K. Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman Latifah Kosim Darusman Luthfiana, Widya M. Rafi Mahat, Mohd Muzamir Maily Mustofa Makmum Syafi’i Manggar Arum Aristri Moh Arif Rohman Nur Hakim Mohamad Rafi Mohamad Rafi Mohamad Rafi Monica Ina Martha Muhammad Bachri Amran Muhammad Farid Munawaroh, Fauziyatul Nevianti Zamany Nisa, Ulfiatun Novik Nurhidayat Nur Zamilah Nurdiani Nurdiani Nursifa Mawadah Ramadhanti, Desi Rany Zalvianita Reni Febriani Ria Apriana Ridhova, Aga Rika Indri Astuti Rizna Azrianiningsari Rudi Heryanto Saat Egra Salmi Afifah Selvitia Ulandari Septaningsih, Dewi Anggraini Setyanto Tri Wahyudi Silvia, Riva Silviani, Dea Siti Aisyah SITI SA’DIAH Sri Purwanti Sri Purwanti Sri Purwanti Sukendi, Yeli Suparto, Irma Syarifah Meutiah Eka Sari Syifa Muflihah Tamida Elinda Thaha, Yudi Nugraha Ulfiatun Nisa Utami Dyah Syafitri Waras Nurcholis Widya Luthfiana Wulansari, Laela Yessie Widya Sari Yogo Suro Priyadi

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