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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Indonesia Chimica Acta Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Chemistry Indonesian Journal of Chemical Research Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Makara Journal of Science Indonesian Journal of Community Engagement Indonesian Journal of Jamu Analit: Analytical And Environmental Chemistry

Wulan Tri Wahyuni

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia

Author-ID : 411386

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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Data Fusion of UV-Vis and FTIR Spectra Combined with Principal Component Analysis for Distinguishing of Andrographis paniculata Extracts Based on Cultivation Ages and Solvent Extraction Antonio Kautsar; Wulan Tri Wahyuni; Utami Dyah Syafitri; Syifa Muflihah; Nursifa Mawadah; Eti Rohaeti; Zulhan Arif; Bambang Prajogo; Muhammad Bachri Amran; Abdul Rohman; Mohamad Rafi
Indonesian Journal of Chemistry Vol 21, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.60321

Andrographis paniculata is one of the medicinal plants used for the treatment of antidiabetic. Cultivation ages and solvent extraction affected metabolites' composition and concentration that directly cause the plant's efficacies. This research aimed to distinguish A. paniculata based on cultivation ages and solvent extraction using data fusion of UV-Vis and FTIR spectra combined with principal component analysis (PCA). A. paniculata with 2, 3, and 4 months post-planting were extracted by water, 50% ethanol, 70% ethanol, and ethanol. In each extract, we measured UV-Vis and FTIR spectra. Then, we used the data fusion from both spectra. We used UV-Vis and FTIR absorbance from 200–400 nm and 1800–400 cm–1, respectively. Each extract gives a similar pattern of UV-Vis and FTIR spectra, only differ in their intensities. PCA score plot in two and three-dimensional showed A. paniculata extracts could be distinguished based on cultivation ages and solvent extraction with a total variance of 86 and 92%, respectively. Furthermore, this study confirms the data fusion of UV-Vis and FTIR spectra could distinguished A. paniculata extracts combined with chemometrics based on cultivation ages and solvent extraction.

Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique Wulan Tri Wahyuni; Rudi Heryanto; Eti Rohaeti; Achmad Fauzi; Budi Riza Putra
Jurnal Kimia Sains dan Aplikasi Vol 24, No 2 (2021): Volume 24 Issue 2 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.

Antioxidant Activity of Daemonorops draco Resin Sri Purwanti; Wulan Tri Wahyuni; Irmanida Batubara
Jurnal Kimia Sains dan Aplikasi Vol 22, No 5 (2019): Volume 22 Issue 5 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

Pembuatan Recycle Screen Printed Carbon Electrode dan Aplikasinya untuk Deteksi Asam Galat dengan Teknik Voltammetri Wulan Tri Wahyuni; Rany Zalvianita; Rudi Heryanto
Jurnal Kimia Sains dan Aplikasi Vol 22, No 5 (2019): Volume 22 Issue 5 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Gallic acid is phenolic compound found in tea and act as antiradical agent. This compound is electrochemically active and could be detected using voltammetric technique. This study aims to obtain recycle screen printed carbon electrode (rSPCE) and applied it for voltammetric detection of gallic acid. rSPCE was prepared by modification of used SPCE working electrode using a mixture of graphite:polystyrene at ratio of 95:5, 90:10, and 85:15 (w/w). Graphite:polystyrene was suspended in chloroform and deposited on SPCE by drop casting method. Phosphate buffer 0.1 M at pH of 2.5, 3.5, and 7 were used as electrolyte solution in voltammetric detection of gallic acid. The optimum condition of gallic acid detection was obtained when phosphate buffer 0,1 M at pH of 2.5 and rSPCE in composition of graphite:polystyrene (95:5) used as electrolyte and working electrode, respectively. Gallic acid has 2 oxidation peaks at potential of 0.26 V and 0.63 V vs Ag/AgCl, respectively. At concentration of 1 – 5 mM, gallic acid and oxidation currents provide linear regression with the coefficient determination of 0.9947 and 0.9864, consecutively for peak number 1 and 2. Measurement of gallic acid at rSPCE 95:5 shows good precision with %RSD < 5%. rSPCE was successfully applied for voltammetric detection of gallic acid in standard solution with accuracy > 96%, however further development is needed before its application for gallic acid measurement in real sample such as tea extract.

Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker Badrunanto Badrunanto; Wulan Tri Wahyuni; Hera Nirwati; Mohamad Rafi
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.

Screening and Optimization Consortium Technique of the Bacillus megatherium and Saccharomyces cerevisiae Microbial Consortium for Ethanol Detection Nurdiani Nurdiani; Dyah Iswantini; Novik Nurhidayat; Wulan Tri Wahyuni; Agus Kartono
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 1, May 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i1.31506

Determination of alcohol content is very important in the food and beverage industry. Biosensor is an alternative to measuring ethanol content. Alcohol biosensors with a single microbe still have a narrow measurement area at ethanol concentrations, so a microbial consortium is needed to widen the range of measured ethanol concentrations. Therefore, it is necessary to screen the microbes from Bacillus sp and S. cerevisiae which have the potential to produce alcohol dehydrogenase (ADH) enzymes and optimize the consortium technique that can provide the best response to oxidation currents. In the yeast microbial screening of 14 S. cerevisiae isolates and 5 Bacillus sp isolates, it can be concluded that for the 14 yeast S. cerevisiae isolates that have the potential to produce ADH enzymes, there is one isolate with the SCRF code. For the 5 bacterial isolates of Bacillus sp that have the potential to produce ADH enzymes, all Bacillus isolates with the code Bacillus megatherium 29/9/14, Bacillus megatherium 23/6/22, Bacillus 6, Bacillus 53, and Bacillus 55. Based on the oxidation current data, Bacillus megatherium 23 /6/22 produces the highest current compared to other Bacillus isolates. The consortium technique that provides the highest current is the method of mixing 1:1 (µL) microbial suspension in an Eppendorf container. Optimization of the consortium's biofilms using the Response Surface Method was produced at 10 days of age, pH 7.5, and 75 µL of microbial suspension dripping volume.

Enhanced Ethanol Production by High Temperature-Tolerance Mutant Pichia kudriavzevii T-T2 in various Carbon and Nitrogen Sources Monica Ina Martha; Rika Indri Astuti; Wulan Tri Wahyuni
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Previously, we had constructed proline accumulating Pichia kudriavzevii yeast mutant (T-T2) that exhibited high temperature tolerance phenotype. In this study we then analyzed the ethanol production of the particular mutant isolate in various carbon (C) and nitrogen sources (N). Fermentation rate of T-T2 either in 4% glucose or substrate mixture (SM = 2%glucose : 2% xylose) was higher than WT strain. The highest CO2 evolving rate by T-T2 in glucose and SM was 57.0 ml/hour and 51.4 ml/hour, respectively, suggesting that T-T2 was capable of using xylose as C-source. T-T2 resulted significant increase of total evolved CO2 than WT strain in glucose as substrate. T-T2 isolate was capable of producing higher ethanol than its WT strains in various C-sources, including glucose, maltose, sucrose, xylose and SM. However, glucose was found as the most preferable C-source to produce ethanol (3.2%) whereas maltose was the least preferable one. T-T2 was found capable in using inorganic sources (NaNO3) to produce ethanol (2.4%) as compared to other N-sources (urea, (NH4)2SO4, NH4Cl). Ethanol production by T-T2 strain in NaNO3 was 16% higher than its WT strain. This data indicate, that NaNO3 is potentially applied as N source alternative to produce ethanol. Based on our study, mutant strain T-T2 shows higher fermentation rate than its WT strain in various sugar and N-sources. The fact that T-T2 could produce ethanol from SM in a similar fermentation rate to single glucose as substrate, suggesting its potential application in the 2nd generation of bioethanol productions.

Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum Irmanida Batubara; Maily Mustofa; Wulan Tri Wahyuni; Kilala Tilaar; Waras Nurcholis; Fransiska Devy Junardy; Yogo Suro Priyadi; Erna Subroto; Saat Egra; Nevianti Zamany
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22676

Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC50 of 393.8 mg/L and IC50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths.Â The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.

Antibacterial and Antibiofilm Activity of Daemonorops draco Resin Wulan Tri Wahyuni; Sri Purwanti; Irmanida Batubara
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13554

Daemonorops draco has been reported for its antibacterial activity and empirically used for wound healing by Anak Dalam ethnic at Jambi Province, Sumatera-Indonesia. This study was performed to evaluate antibacterial and antibiofilm activity of D. draco resin collected from Jambi. D. draco resin was extracted using n-hexane, ethyl acetate and methanol, respectively. Antibacterial activity of the extracts was evaluated using agar diffusion method against Staphylococcus aureus and Eschericia coli, whereas the minimum inhibitory concentration (MIC) and minimum bacteriacidal concentration (MBC) was determined by microdilution method. In addition, antibiofilm activity was evaluated by violet crystal method. The result showed that extraction yield of ethyl acetate was higher than methanol and n-hexane. Ethyl acetate and methanol extracts of D. draco exhibited stronger antimicrobial activity against S. aureus compare to n-hexane extract. MIC and MBC of methanol extract and chromatographic fraction (F5.1) of ethyl acetate extract were 0.5 and 1.0 mg/mL, respectively. In addition, antibiofilm assay revealed that all extracts were inhibit initial attachment of bacteria cell in biofilm formation. This result revealed a novel information that Â D. draco extracts was potential as inhibitor of biofilm formation. TLC bioautography of D. draco extracts indicated that constituent with Rf of 0.71 performed antimicrobial activity against S. aureus. This finding expected to strengthen the scientific backup for utilization of D. draco by society.

An Inkjet-printed Graphene Oxide–poly(3,4-ethylenedioxythiophene) poly(styrene sulfonate) Electrode for Nitrite Detection in Water Putra, Budi Riza; Anindya, Weni; Rafi, Mohamad; Kartika, Ika; Thaha, Yudi Nugraha; Ridhova, Aga; Wahyuni, Wulan Tri
Makara Journal of Science Vol. 27, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

In this study, a screen-printed electrode (SPE) for nitrite (NO2−) sensing was fabricated through an inkjet printing technique using a commercial printer machine and preparing an ink composite solution containing graphene oxide (GO) and poly(3,4 ethylenedioxythiophene) poly(styrene sulfonate) (PEDOT:PSS) as conductive materials. The optimum ink materials for SPE fabrication comprised GO and PEDOT:PSS with binders that resemble the viscosity of commercial ink. The surface topography of the fabricated SPE, which was characterized using scanning electron microscopy and atomic force microscopy, showed a smoother surface compared to the commercial screen-printed carbon electrode, with conductive ink materials deposited primarily from GO/PEDOT:PSS composites. This NO2− sensor exhibited a linear response at the concentration range of 50–1000 µM, with limit of detection and limit of quantification as 25 and 50 µM, respectively. In addition, the stability, reproducibility, and selectivity of this sensor presented acceptable values in the analytical range. Furthermore, the performance of this sensor was compared with that of a spectrophotometry technique using synthetic water samples, and the results revealed its good analytical recovery at a confidence interval of 95%. Thus, our sensor based on SPE-modified GO/PEDOT:PSS, fabricated via the inkjet printing technique, can be potentially applied in monitoring NO2− concentration in real samples.

Co-Authors Abdul Rohman Achmad Fauzi Achmad Fauzi Aditya Widianto Afifah, Salmi Agus Kartono Ahmad, Shahrul Nizam Aji Hamim Wigena Aninda Naszwa Kuswanto Anindya, Weni Antonio Kautsar Apriana, Ria Apriliani Rahmat Asoka, Shadila Fira Badrunanto Badrunanto Badrunanto, Badrunanto Bambang Prajogo Eko Wardojo Budi Riza Putra Budi Riza Putra Budi Riza Putra Darusman Pitria, Latifah K Desi Herliani Dinda Iryawati Dyah Iswantini Eka Widya Nugraha Era Rahmi Erna Subroto Eti Rohaeti Fransiska Devy Junardy Hera Nirwati Herdiyanto Herdiyanto Herdiyanto Herdiyanto Herliani, Desi Hidayat, Rayyan Azzahra Ika Kartika Irma Herawati Suparto Irmanida Batubara Irmanida Batubara Karomah, Alfi Hudatul Kilala Tilaar Kuswanto, Aninda Naszwa Latifah K Darusman latifah K Darusman Latifah K Darusman Latifah K Darusman Pitria Latifah K. Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman Latifah Kosim Darusman Luthfiana, Widya M. Rafi Mahat, Mohd Muzamir Maily Mustofa Makmum Syafi’i Manggar Arum Aristri Martha, Ina Moh Arif Rohman Nur Hakim Moh Nur Hakim Mohamad Rafi Muhammad Bachri Amran Muhammad Farid Munawar Khalil Munawaroh, Fauziyatul Nevianti Zamany Nisa, Ulfiatun Novik Nurhidayat Nur Zamilah Nurdiani Nurdiani Nursifa Mawadah Rahmat, Apriliani Ramadhanti, Desi Rany Zalvianita Reni Febriani Reni Febriani, Reni Ria Apriana Ridhova, Aga Rika Indri Astuti Rizna Azrianiningsari Rudi Heryanto Saat Egra Salmi Afifah Selvitia Ulandari Septaningsih, Dewi Anggraini Setyanto Tri Wahyudi Silvia, Riva Silviani, Dea Siti Aisyah SITI SA’DIAH Sri Purwanti Sri Purwanti Sri Purwanti Sukendi, Yeli Suparto, Irma Syafi’i, Makmum Syarifah Meutiah Eka Sari Syifa Muflihah Tamida Elinda Thaha, Yudi Nugraha Trivadila Wahyuni Ulfiatun Nisa Ulfiatun Nisa Utami Dyah Syafitri Waras Nurcholis Widianto, Aditya Wulansari, Laela Yessie Widya Sari Yogo Suro Priyadi Yusuf Ryadi Zamilah, Nur

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