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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Indonesia Chimica Acta Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Chemistry Indonesian Journal of Chemical Research Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Makara Journal of Science Indonesian Journal of Community Engagement Indonesian Journal of Jamu Analit: Analytical And Environmental Chemistry
Wulan Tri Wahyuni
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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A Brief Review on Fabrication of Screen-Printed Carbon Electrode: Materials and Techniques Wahyuni, Wulan Tri; Putra, Budi Riza; Fauzi, Achmad; Ramadhanti, Desi; Rohaeti, Eti; Heryanto, Rudi
Indonesian Journal of Chemical Research Vol 8 No 3 (2021): Edition for January 2021
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2021.8-wul

Abstract

Screen-printed carbon electrode (SPCE) is one of the most interesting designs to combine a working (from carbon based material), reference, and counter electrode in a single-printed substrate. SPCE has been used in many electrochemical measurements due to its advantages for analysis in microscale. This paper summarises the main information about SPCE fabrication from the material and fabrication technique aspect on the flat substrate based on the work that has been published in the last 30 years. The success of SPCE fabrication is highly dependent on the composition of conductive ink which consists of conductive materials, binder, and solvents; substrate; and fabrication techniques. Among the carbon-based materials, the most widely used for SPCE fabrications are graphite, graphene, and carbon nanotubes. The frequent binder used are polymer-based materials such as polystyrene, polyaniline, poly 3,4-ethylenedioxythiophene:polystyrene sulfonate (PEDOT:PSS), and polyvinyl chloride. The solvents used for SPCE fabrication are varied including water and various organic solvents. The main characteristics of the SPCE substrate should be inert in order to avoid any interferences during electrochemical measurements. The screen printing and inkjet printing technique are preferred for SPCE fabrication due to easy fabrication and the possibility for mass production of SPCE.
Development of Differential Pulse Anodic Stripping Voltammetry Technique for Cadmium(II) Detection and Its Application in Water Spinach Putra, Budi Riza; Aristri, Manggar Arum; Rohaeti, Eti; Wahyuni, Wulan Tri
Indonesian Journal of Chemical Research Vol 9 No 2 (2021): Edition for September 2021
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2021.9-riz

Abstract

Cadmium is a toxic pollutant that is harmful to the environment and humans. The purpose of this research was to develop a method for cadmium(II) detection using differential pulse anodic stripping voltammetry (DPASV) using a glassy carbon electrode. The developed method was then applied for cadmium detection in the vegetable samples which is water spinach. The developed method was optimized in several parameters such as potential window, deposition potential, deposition time, and scan rate. The developed method for cadmium(II) detection was also investigated in its analytical performance includes linearity, precision, detection limit, and quantitation limit. The optimum conditions for cadmium(II) detection in 0.1 M KCl using DPASV technique obtained such as potential window from -1200 to -100 mV, deposition potential of -1100 mV (vs Ag/AgCl), and deposition time of 360 s. It was obtained good linearity for cadmium(II) detection using the DPASV technique with an R2 of 0.996. The precision was expressed as %SBR with 0.66%. The detection and quantitation limits for cadmium(II) detection were 0.4206 µM~0.0771 ppm and 0.5525 µM~0.1013 ppm, respectively. The developed method was then applied for cadmium(II) measurement in the water spinach sample and the obtained cadmium(II) concentration in water spinach was 0.2399 mg/Kg.
Component Discrimination and Anti-skin-aging Potency of Emprit and Red Ginger Essential Oil: Chemometric, Molecular Docking and Molecular Dynamics Study Badrunanto, Badrunanto; Asoka, Shadila Fira; Wahyuni, Wulan Tri; Farid, Muhammad; Wahyudi, Setyanto Tri; Batubara, Irmanida
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 2, November 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i2.32765

Abstract

Emprit and red ginger essential oils (EOs) are natural sources of antioxidants that have the potential to be used in cosmetics, one of which is as an anti-skin-aging. The aim of this study was to determine the component differences and anti-skin-aging potential of the two EOs. The components were determined by GC-MS, while discrimination was done by chemometric. The potential of the components as the anti-skin-aging were evaluated by molecular docking and molecular dynamics (MD) simulations. A total 66 components were identified in both EOs, where eucalyptol (17.92%) and camphene (15.12%) were the main component in emprit and red ginger, respectively. Chemometric analysis revealed two discriminant clusters highlighting their dissimilarity with germacrene D and α-zingiberene are the key markers for differentiation. The docking and MD simulations were demonstrated the four main components of emprit EO, namely α-curcumene, α-zingiberene, β-bisabolene and β-sesquiphellandrene, have the best docking scores and interact with the enzymes with a relatively stable interaction. AdmetSAR evaluation of the four components has shown good bioavailability and declared safe. This study succeeded in revealing two ginger EOs differences based on their components and demonstrated the emprit ginger EO was more promising as a natural anti-skin-aging agent for further research.
Correlation between Time Variation of Steam Distillation of Temu Hitam’s Rhizomes (Curcuma aeruginosa) and Antibacterial Activity of Essential Oil Munawaroh, Fauziyatul; Luthfiana, Widya; Suparto, Irma Herawati; Sari, Yessie Widya; Wahyuni, Wulan Tri; Batubara, Irmanida
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.345

Abstract

Distillation time can affect the extraction of essential oils such as the rhizome of temu hitam (Curcuma aeruginosa) which has been shown to have antibacterial activity. Therefore, this study aims to determine the optimum distillation time to produce temu hitam rhizome oil with the strongest antibacterial activity. The rhizomes of temu hitam were steam distilled with different length of time every 30 minutes for 4 hours to obtain essential oil and determined the compounds contained in them using gas chromatography-mass spectrometry. Antibacterial activity was determined by disc diffusion against Streptococcus mutans, Escherichia coli, and Staphylococcus aureus. The most dominant compounds present in essential oils are eucalyptol (34.89%), epicurzerenone (20.58%), and camphor (16.90%) based on the average peak of the total distillation time. The strongest antibacterial potency was obtained in the first 30 minutes of distillation for S. mutans and E. coli, and in the seventh 30 minutes of distillation for S. aureus.
LC-HRMS-Based Metabolomics Approach Reveals Antioxidant Compounds from Centella asiatica Leaves Extracts Silvia, Riva; Wahyuni, Wulan Tri; Rohaeti, Eti; Aisyah, Siti; Septaningsih, Dewi Anggraini; Karomah, Alfi Hudatul; Rafi, Mohamad
Indonesian Journal of Chemistry Vol 24, No 6 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90782

Abstract

Centella asiatica is a medicinal plant widely used as a traditional medicine due to several biological activities, such as antioxidants in Indonesia. This study aims to identify the active antioxidant compounds of C. asiatica leaves extract using a liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)-based metabolomics approach. Extracts were prepared using different concentrations of ethanol p.a., i.e. 70% ethanol, 50% ethanol, 30% ethanol, and water. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl method. The results showed the highest antioxidant activity was C. asiatica extracted by 70% ethanol with IC50 of 72.48 ± 2.42 µg/mL. The positive control was ascorbic acid, having an IC50 value of 3.38 ± 0.04 µg/mL. Ascorbic acid and 70% ethanol extract have strong antioxidant activity. Metabolite profiling using LC-MS/MS could identify 35 metabolites consisting of flavonoids, fatty acids, phenolics, terpenes, and several other groups of compounds. Orthogonal partial least squares-discriminant analysis classified the metabolites into active (ethanol 70% and ethanol 50%) and inactive (ethanol p.a., ethanol 30%, and water) antioxidants. Five metabolites have potential as antioxidants, namely 4,5-dicaffeoylquinic acid (11), kaempferol (13), and three unknown compounds.
Nonenzymatic Sensor Based on Glassy Carbon Electrode Modified by Platinum Nanoparticles Decorated Reduced Graphene Oxide for Glucose Detection in Human Urine Nisa, Ulfiatun; Iswantini, Dyah; Ahmad, Shahrul Nizam; Mahat, Mohd Muzamir; Putra, Budi Riza; Saskito, Dinda Iryawati Bedy; Wahyuni, Wulan Tri
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 2, November 2024
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i2.40035

Abstract

This research aims to develop a sensitive and selective nonenzymatic electrochemical sensor for glucose detection using a glassy carbon electrode modified with platinum nanoparticles (PtNPs) decorated on reduced graphene oxide (RGO). The structural properties and surface morphology of PtNPs/RGO composite were characterized using Raman spectroscopy and scanning electron microscopy (SEM). In addition, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were employed to investigate glucose measurements in human urine samples. The developed sensor shows an increasing anodic peak of glucose with a linear response at a concentration range from 10 to 1000 µM, with a detection limit of 5 µM. The proposed sensor also demonstrated good reproducibility, indicated by the value of relative standard deviation (%RSD) of 3.9%, and maintained its current response over seven consecutive measurements. Moreover, the proposed sensor exhibited high selectivity for glucose detection against several potential interferences, such as potassium (K+), chloride (Cl−), magnesium (Mg2+), ascorbic acid, dopamine, and urea, with recovery values of 96-102%, which are acceptable within the analytical range. Furthermore, this proposed sensor successfully detected glucose in human urine samples, and their concentrations were not significantly different when measured with a commercial glucose sensor.
Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit Syarifah Meutiah Eka Sari; Suparto, Irma; Wulan Tri Wahyuni
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.131

Abstract

Guava crystal is a fruit that has a high selling value. One of the obstacles to marketing crystal guava fruit is the fast decay process. Many factors cause fruit to rot quickly, including microbial contamination post-harvesting. This study evaluates the effectiveness of adding lemongrass oil in chitosan coatings to protect crystal guava from microbiological damage during storage. The fruit is coated with 1% chitosan and added to lemongrass oil according to the treatment. The study used a completely randomized design with three replications. The treatment tested included 0.0, 0.1, 0.3, and 0.5% lemongrass oil. The content of lemongrass oil compounds was analyzed using gas chromatography-mass spectroscopy (GC-MS). The antibacterial test was carried out using the disc method. The parameters observed were the total plate count and total fungal count of crystal guava fruit on days 0, 3, 6, 9, 12, 15, and 18 after storage. GC-MS analysis showed that the main content of lemongrass oil is two isomers of citral, i.e., neral (38.54%) and geranial (39.26%). The results showed that the total fungal and plate count increased during storage. Chitosan coating with lemongrass oil more effectively inhibits Staphylococcus aureus than Escherichia coli. Adding 0.3% and 0.5% lemongrass oil in chitosan could restrain the rate of microbes in crystal guava until the 18th day after storage. Chitosan treatment with 0,3% lemongrass oil is recommended to be the best treatment for applying on crystal guava. Keywords: citral, GC-MS, Psidium guajava L., total plate count, total fungal count
Traditional Uses, Biological Activities, and Phytochemical Profile of Keji Beling (Strobilanthes crispus) Leaf Extract: A Review Sukendi, Yeli; Rafi, Mohamad; Silviani, Dea; Wahyuni, Wulan Tri
Jurnal Jamu Indonesia Vol. 10 No. 1 (2025): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v10i1.305

Abstract

Health treatment in Indonesia is diverse, encompassing both modern medicines using synthetic drugs and traditional practices relying on herbal plants. Traditional medicine is often preferred due to its lower cost and perceived lack of side effects. Keji beling (Strobilanthes crispus) is one such herb with potential medicinal properties. This article summarizes information regarding traditional uses, biological activities, phytochemical profiles, and active compounds of keji beling leaf extract. Traditionally, keji beling leaves are consumed as tea. The plant has demonstrated antioxidant, anti-diabetic, anticancer, antitumor, and antibacterial properties. These biological effects are attributed to its phytochemical constituents, including flavonoids, alkaloids, saponins, tannins, and terpenoids. Specific bioactive compounds in keji beling leaf extract comprise silicic acid, polyphenols, glycosides, catechins, rutin, epicatechin, myricetin, luteolin, apigenin, naringenin, and kaempferol.
TLC Fingerprint Analysis and Evaluation of α-Glucosidase Inhibitory and Free Radical Scavenging Activity of Justicia gendarussa from Different Growth Locations Wulansari, Laela; Wahyuni, Wulan Tri; Nurcholis, Waras; Rafi, Mohamad
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.43662

Abstract

Justicia gendarussa is known to have antioxidant and antidiabetic properties, which are related to the composition and concentration of its metabolites and influenced by differences in growth location. This study aims to evaluate changes in metabolite profiles and biological activities, such as the inhibition of α-glucosidase and free radical scavenging in J. gendarussa genotypes from Bogor, Cianjur, and Sukabumi. Changes in metabolite profiles were assessed using thin-layer chromatography (TLC) fingerprint analysis combined with principal component analysis (PCA). Bioautography TLC using DPPH was performed to confirm the presence of antioxidant compounds. In the TLC fingerprint analysis of J. gendarussa, we obtained 14 bands with good resolution. PCA successfully grouped J. gendarussa extracts based on growth location. The percentage of α-glucosidase inhibitory and free radical scavenging activity was significantly different, with the highest percentage of inhibition of α-glucosidase shown by the IIIS genotype (99.23%) and the highest free radical scavenging (91.35%) demonstrated by the IIB genotype. The results of TLC bioautography confirmed the presence of antioxidant compounds in J. gendarussa represented by bands with Rf 0.04, 0.32, and 0.80. This study concludes that growth location differences influence the variations in metabolite profiles, α-glucosidase inhibitory activity, and free radical scavenging activity of J. gendarussa genotypes.
Metode Ekstraksi dan Pemisahan Optimum Untuk Isolasi Xantorizol dari Temulawak (Curcuma xanthorrhiza) Wahyuni, Wulan Tri; Herdiyanto, Herdiyanto; Rafi, Mohamad
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i2.31

Abstract

Xantorizol merupakan senyawa penciri utama temulawak (Curcuma xanthorrhiza). Penelitian ini bertujuan menentukan metode ekstraksi dan pemisahan optimum untuk isolasi xantorizol dari rimpang temulawak. Maserasi dan sokletasi digunakan untuk mengekstraksi xantorizol dengan pelarut metanol, dietil eter, dan n-heksana. Pemisahan dilakukan dengan kromatografi kolom dan kromatografi lapis tipis (KLT) preparatif dan hasil pemisahan dikarakterisasi berdasarkan spektrum inframerah dan kromatografi gas-spektrum massa (KG-SM), sementara persentase kemurniannya ditentukan dengan kromatografi cair kinerja tinggi. Ekstrak n-heksana dari teknik maserasi memiliki kandungan xantorizol lebih tinggi dibanding ekstrak lainnya yaitu sebesar 168 mg/g sampel. Fraksi ke-4 hasil pemisahan kolom terhadap ekstrak n-heksana memberikan dua spot pada KLT dengan Rf 0.54 dan 0.68, spot dengan Rf 0.54 diduga merupakan xantorizol (dikonfirmasi dengan KG-SM). Pemurnian lebih lanjut dengan KLT preparatif terhadap fraksi ke-4 menghasilkan xantorizol dengan rendemen sebesar 0.016 % berdasar bobot sampel dan tingkat kemurnian sebesar 87.40 %.
Co-Authors Abdul Rohman Achmad Fauzi Achmad Fauzi Aditya Widianto Afifah, Salmi Agus Kartono Ahmad, Shahrul Nizam Aji Hamim Wigena Aninda Naszwa Kuswanto Anindya, Weni Antonio Kautsar Apriana, Ria Apriliani Rahmat Asoka, Shadila Fira Badrunanto Badrunanto Badrunanto, Badrunanto Bambang Prajogo Eko Wardojo Budi Riza Putra Budi Riza Putra Budi Riza Putra Darusman Pitria, Latifah K Desi Herliani Dinda Iryawati Dyah Iswantini Eka Widya Nugraha Era Rahmi Erna Subroto Eti Rohaeti Fransiska Devy Junardy Hera Nirwati Herdiyanto Herdiyanto Herdiyanto Herdiyanto Herliani, Desi Hidayat, Rayyan Azzahra Ika Kartika Irma Herawati Suparto Irmanida Batubara Irmanida Batubara Karomah, Alfi Hudatul Kilala Tilaar Kuswanto, Aninda Naszwa latifah K Darusman Latifah K Darusman Latifah K Darusman Latifah K Darusman Pitria Latifah K. Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman Latifah Kosim Darusman Luthfiana, Widya M. Rafi Mahat, Mohd Muzamir Maily Mustofa Makmum Syafi’i Manggar Arum Aristri Martha, Ina Moh Arif Rohman Nur Hakim Moh Nur Hakim Mohamad Rafi Muhammad Bachri Amran Muhammad Farid Munawar Khalil Munawaroh, Fauziyatul Nevianti Zamany Nisa, Ulfiatun Novik Nurhidayat Nur Zamilah Nurdiani Nurdiani Nursifa Mawadah Rahmat, Apriliani Ramadhanti, Desi Rany Zalvianita Reni Febriani Reni Febriani, Reni Ria Apriana Ridhova, Aga Rika Indri Astuti Rizna Azrianiningsari Rudi Heryanto Saat Egra Salmi Afifah Selvitia Ulandari Septaningsih, Dewi Anggraini Setyanto Tri Wahyudi Silvia, Riva Silviani, Dea Siti Aisyah SITI SA’DIAH Sri Purwanti Sri Purwanti Sri Purwanti Sukendi, Yeli Suparto, Irma Syafi’i, Makmum Syarifah Meutiah Eka Sari Syifa Muflihah Tamida Elinda Thaha, Yudi Nugraha Trivadila Wahyuni Ulfiatun Nisa Ulfiatun Nisa Utami Dyah Syafitri Waras Nurcholis Widianto, Aditya Wulansari, Laela Yessie Widya Sari Yogo Suro Priyadi Yusuf Ryadi Zamilah, Nur