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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Indonesia Chimica Acta Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Chemistry Indonesian Journal of Chemical Research Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Makara Journal of Science Analit: Analytical and Environmental Chemistry Indonesian Journal of Jamu

Wulan Tri Wahyuni

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia

Author-ID : 411386

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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ANALISIS KADAR FLAVONOID DAN ANTIOKSIDAN EKSTRAK DAUN KENIKIR (Cosmos caudatus), RUMPUT MUTIARA (Oldenlandia corymbosa), DAN SIRSAK (Annona muricata) DENGAN TEKNIK SPEKTROMETRI Wulan Tri Wahyuni; Latifah K Darusman Pitria; Apriliani Rahmat
Analit: Analytical and Environmental Chemistry Vol 3, No 1 (2018): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Pada penelitian ini dilakukan analisis kadar senyawa flavonoid dan penentuan aktivitas antioksidan dari ekstrak etanol daun kenikir (Cosmos caudatus), rumput mutiara (Oldenlandia corymbosa), dan sirsak (Annona muricata) dengan teknik spektrometri. Ekstrak masing-masing sampel diperoleh melalui ekstraksi dengan bantuan gelombang ultrasonik. Kadar flavonoid ditentukan dengan teknik spektrometri menggunakan kuersetin sebagai standar, sementara aktivitas antioksidan dievaluasi melalui penangkapan radikal bebas 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasil yang diperoleh menunjukkan bahwa rendemen ekstraksi daun sirsak lebih tinggi dibandingkan rumput mutiara dan kenikir. Ekstrak etanol sirsak memiliki aktivitas penangkapan radikal bebas DPPH terbaik dengan nilai IC50 sebesar 37.91 µg/mL. Kadar flavonoid total dari ekstrak etanol sirsak tersebut ialah sebesar 1.78 mg ekuivalen kuersetin/mg sampel, lebih rendah dibandingkan kadar flavonoid rumput mutiara dan kenikir. Hasil ini mengindikasikan bahwa aktivitas antioksidan pada ekstrak daun sirsak dimungkinkan tidak hanya berasal dari flavonoid, namun berasal dari gabungan senyawa flavonoid dengan senyawa aktif lain yang dikandungnya DOI: http://dx.doi.org/10.23960/aec.v3.i1.2018.p38-46

MODIFIKASI SCREEN PRINTED CARBON ELECTRODE DENGAN POLIMER TERCETAK MOLEKUL (MIP) DAN APLIKASINYA UNTUK DETEKSI GLUTATION Eti Rohaeti; Wulan Tri Wahyuni; Moh Arif Rohman Nur Hakim
Analit: Analytical and Environmental Chemistry Vol 3, No 1 (2018): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Penelitian ini bertujuan memodifikasi screen printed carbon electrode (SPCE) dengan polimer tercetak molekul (MIP) dan mengaplikasikannya untuk deteksi glutation tereduksi (GSH) secara tidak langsung dengan mediator bromin menggunakan teknik voltammetri siklik. SPCE dimodifikasi dengan MIP polipirola (MIPpy) melalui teknik elektrodeposisi. Pengukuran GSH dilakukan dalam elektrolit NaClO4 0.2 M yang mengandung 1 mM NaBr. Hasil yang diperoleh menunjukkan SPCE-MIPpy telah berhasil diperoleh melalui teknik elektrodeposisi. Pada pengukuran diperoleh puncak oksidasi bromida pada potensial ±0.63 V vs. Ag/AgCl. Puncak ini berkorelasi linear dengan konsentrasi GSH dalam larutan sehingga dapat digunakan sebagai dasar penentuan GSH. Polipirola meningkatkan konduktivitas listrik elektrode sehingga dapat meningkatkan puncak arus oksidasi GSH. Pengukuran glutation dengan SPCE-MIPpy menunjukkan limit deteksi dan limit kuantitasi yang lebih baik dibandingkan SPCE, yaitu sebesar 7.6626 µM dan 25.4092 µM. Keberadaan interference glukosa tidak mengganggu pengukuran glutation. Kinerja SPCE-MIPpy dalam pengukuran GSH disimpulkan lebih optimum dibandingkan SPCE. DOI: http://dx.doi.org/10.23960/aec.v3.i1.2018.p47-60

Enhanced Ethanol Production by High Temperature-Tolerance Mutant Pichia kudriavzevii T-T2 in various Carbon and Nitrogen Sources Monica Ina Martha; Rika Indri Astuti; Wulan Tri Wahyuni
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Previously, we had constructed proline accumulating Pichia kudriavzevii yeast mutant (T-T2) that exhibited high temperature tolerance phenotype. In this study we then analyzed the ethanol production of the particular mutant isolate in various carbon (C) and nitrogen sources (N). Fermentation rate of T-T2 either in 4% glucose or substrate mixture (SM = 2%glucose : 2% xylose) was higher than WT strain. The highest CO2 evolving rate by T-T2 in glucose and SM was 57.0 ml/hour and 51.4 ml/hour, respectively, suggesting that T-T2 was capable of using xylose as C-source. T-T2 resulted significant increase of total evolved CO2 than WT strain in glucose as substrate. T-T2 isolate was capable of producing higher ethanol than its WT strains in various C-sources, including glucose, maltose, sucrose, xylose and SM. However, glucose was found as the most preferable C-source to produce ethanol (3.2%) whereas maltose was the least preferable one. T-T2 was found capable in using inorganic sources (NaNO3) to produce ethanol (2.4%) as compared to other N-sources (urea, (NH4)2SO4, NH4Cl). Ethanol production by T-T2 strain in NaNO3 was 16% higher than its WT strain. This data indicate, that NaNO3 is potentially applied as N source alternative to produce ethanol. Based on our study, mutant strain T-T2 shows higher fermentation rate than its WT strain in various sugar and N-sources. The fact that T-T2 could produce ethanol from SM in a similar fermentation rate to single glucose as substrate, suggesting its potential application in the 2nd generation of bioethanol productions.

Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum Irmanida Batubara; Maily Mustofa; Wulan Tri Wahyuni; Kilala Tilaar; Waras Nurcholis; Fransiska Devy Junardy; Yogo Suro Priyadi; Erna Subroto; Saat Egra; Nevianti Zamany
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22676

Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC50 of 393.8 mg/L and IC50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths.Â The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.

Antibacterial and Antibiofilm Activity of Daemonorops draco Resin Wulan Tri Wahyuni; Sri Purwanti; Irmanida Batubara
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13554

Daemonorops draco has been reported for its antibacterial activity and empirically used for wound healing by Anak Dalam ethnic at Jambi Province, Sumatera-Indonesia. This study was performed to evaluate antibacterial and antibiofilm activity of D. draco resin collected from Jambi. D. draco resin was extracted using n-hexane, ethyl acetate and methanol, respectively. Antibacterial activity of the extracts was evaluated using agar diffusion method against Staphylococcus aureus and Eschericia coli, whereas the minimum inhibitory concentration (MIC) and minimum bacteriacidal concentration (MBC) was determined by microdilution method. In addition, antibiofilm activity was evaluated by violet crystal method. The result showed that extraction yield of ethyl acetate was higher than methanol and n-hexane. Ethyl acetate and methanol extracts of D. draco exhibited stronger antimicrobial activity against S. aureus compare to n-hexane extract. MIC and MBC of methanol extract and chromatographic fraction (F5.1) of ethyl acetate extract were 0.5 and 1.0 mg/mL, respectively. In addition, antibiofilm assay revealed that all extracts were inhibit initial attachment of bacteria cell in biofilm formation. This result revealed a novel information that Â D. draco extracts was potential as inhibitor of biofilm formation. TLC bioautography of D. draco extracts indicated that constituent with Rf of 0.71 performed antimicrobial activity against S. aureus. This finding expected to strengthen the scientific backup for utilization of D. draco by society.

An Inkjet-printed Graphene Oxide–poly(3,4-ethylenedioxythiophene) poly(styrene sulfonate) Electrode for Nitrite Detection in Water Putra, Budi Riza; Anindya, Weni; Rafi, Mohamad; Kartika, Ika; Thaha, Yudi Nugraha; Ridhova, Aga; Wahyuni, Wulan Tri
Makara Journal of Science Vol. 27, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

In this study, a screen-printed electrode (SPE) for nitrite (NO2−) sensing was fabricated through an inkjet printing technique using a commercial printer machine and preparing an ink composite solution containing graphene oxide (GO) and poly(3,4 ethylenedioxythiophene) poly(styrene sulfonate) (PEDOT:PSS) as conductive materials. The optimum ink materials for SPE fabrication comprised GO and PEDOT:PSS with binders that resemble the viscosity of commercial ink. The surface topography of the fabricated SPE, which was characterized using scanning electron microscopy and atomic force microscopy, showed a smoother surface compared to the commercial screen-printed carbon electrode, with conductive ink materials deposited primarily from GO/PEDOT:PSS composites. This NO2− sensor exhibited a linear response at the concentration range of 50–1000 µM, with limit of detection and limit of quantification as 25 and 50 µM, respectively. In addition, the stability, reproducibility, and selectivity of this sensor presented acceptable values in the analytical range. Furthermore, the performance of this sensor was compared with that of a spectrophotometry technique using synthetic water samples, and the results revealed its good analytical recovery at a confidence interval of 95%. Thus, our sensor based on SPE-modified GO/PEDOT:PSS, fabricated via the inkjet printing technique, can be potentially applied in monitoring NO2− concentration in real samples.

A Brief Review on Fabrication of Screen-Printed Carbon Electrode: Materials and Techniques Wahyuni, Wulan Tri; Putra, Budi Riza; Fauzi, Achmad; Ramadhanti, Desi; Rohaeti, Eti; Heryanto, Rudi
Indonesian Journal of Chemical Research Vol 8 No 3 (2021): Edition for January 2021
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2021.8-wul

Screen-printed carbon electrode (SPCE) is one of the most interesting designs to combine a working (from carbon based material), reference, and counter electrode in a single-printed substrate. SPCE has been used in many electrochemical measurements due to its advantages for analysis in microscale. This paper summarises the main information about SPCE fabrication from the material and fabrication technique aspect on the flat substrate based on the work that has been published in the last 30 years. The success of SPCE fabrication is highly dependent on the composition of conductive ink which consists of conductive materials, binder, and solvents; substrate; and fabrication techniques. Among the carbon-based materials, the most widely used for SPCE fabrications are graphite, graphene, and carbon nanotubes. The frequent binder used are polymer-based materials such as polystyrene, polyaniline, poly 3,4-ethylenedioxythiophene:polystyrene sulfonate (PEDOT:PSS), and polyvinyl chloride. The solvents used for SPCE fabrication are varied including water and various organic solvents. The main characteristics of the SPCE substrate should be inert in order to avoid any interferences during electrochemical measurements. The screen printing and inkjet printing technique are preferred for SPCE fabrication due to easy fabrication and the possibility for mass production of SPCE.

Development of Differential Pulse Anodic Stripping Voltammetry Technique for Cadmium(II) Detection and Its Application in Water Spinach Putra, Budi Riza; Aristri, Manggar Arum; Rohaeti, Eti; Wahyuni, Wulan Tri
Indonesian Journal of Chemical Research Vol 9 No 2 (2021): Edition for September 2021
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2021.9-riz

Cadmium is a toxic pollutant that is harmful to the environment and humans. The purpose of this research was to develop a method for cadmium(II) detection using differential pulse anodic stripping voltammetry (DPASV) using a glassy carbon electrode. The developed method was then applied for cadmium detection in the vegetable samples which is water spinach. The developed method was optimized in several parameters such as potential window, deposition potential, deposition time, and scan rate. The developed method for cadmium(II) detection was also investigated in its analytical performance includes linearity, precision, detection limit, and quantitation limit. The optimum conditions for cadmium(II) detection in 0.1 M KCl using DPASV technique obtained such as potential window from -1200 to -100 mV, deposition potential of -1100 mV (vs Ag/AgCl), and deposition time of 360 s. It was obtained good linearity for cadmium(II) detection using the DPASV technique with an R2 of 0.996. The precision was expressed as %SBR with 0.66%. The detection and quantitation limits for cadmium(II) detection were 0.4206 ÂµM~0.0771 ppm and 0.5525 ÂµM~0.1013 ppm, respectively. The developed method was then applied for cadmium(II) measurement in the water spinach sample and the obtained cadmium(II) concentration in water spinach was 0.2399 mg/Kg.

Component Discrimination and Anti-skin-aging Potency of Emprit and Red Ginger Essential Oil: Chemometric, Molecular Docking and Molecular Dynamics Study Badrunanto, Badrunanto; Asoka, Shadila Fira; Wahyuni, Wulan Tri; Farid, Muhammad; Wahyudi, Setyanto Tri; Batubara, Irmanida
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 2, November 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i2.32765

Emprit and red ginger essential oils (EOs) are natural sources of antioxidants that have the potential to be used in cosmetics, one of which is as an anti-skin-aging. The aim of this study was to determine the component differences and anti-skin-aging potential of the two EOs. The components were determined by GC-MS, while discrimination was done by chemometric. The potential of the components as the anti-skin-aging were evaluated by molecular docking and molecular dynamics (MD) simulations. A total 66 components were identified in both EOs, where eucalyptol (17.92%) and camphene (15.12%) were the main component in emprit and red ginger, respectively. Chemometric analysis revealed two discriminant clusters highlighting their dissimilarity with germacrene D and α-zingiberene are the key markers for differentiation. The docking and MD simulations were demonstrated the four main components of emprit EO, namely α-curcumene, α-zingiberene, β-bisabolene and β-sesquiphellandrene, have the best docking scores and interact with the enzymes with a relatively stable interaction. AdmetSAR evaluation of the four components has shown good bioavailability and declared safe. This study succeeded in revealing two ginger EOs differences based on their components and demonstrated the emprit ginger EO was more promising as a natural anti-skin-aging agent for further research.

Correlation between Time Variation of Steam Distillation of Temu Hitam’s Rhizomes (Curcuma aeruginosa) and Antibacterial Activity of Essential Oil Munawaroh, Fauziyatul; Luthfiana, Widya; Suparto, Irma Herawati; Sari, Yessie Widya; Wahyuni, Wulan Tri; Batubara, Irmanida
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.345

Distillation time can affect the extraction of essential oils such as the rhizome of temu hitam (Curcuma aeruginosa) which has been shown to have antibacterial activity. Therefore, this study aims to determine the optimum distillation time to produce temu hitam rhizome oil with the strongest antibacterial activity. The rhizomes of temu hitam were steam distilled with different length of time every 30 minutes for 4 hours to obtain essential oil and determined the compounds contained in them using gas chromatography-mass spectrometry. Antibacterial activity was determined by disc diffusion against Streptococcus mutans, Escherichia coli, and Staphylococcus aureus. The most dominant compounds present in essential oils are eucalyptol (34.89%), epicurzerenone (20.58%), and camphor (16.90%) based on the average peak of the total distillation time. The strongest antibacterial potency was obtained in the first 30 minutes of distillation for S. mutans and E. coli, and in the seventh 30 minutes of distillation for S. aureus.

Co-Authors Abdul Rohman Achmad Fauzi Achmad Fauzi Aditya Widianto Agus Kartono Ahmad, Shahrul Nizam Aji Hamim Wigena Aninda Naszwa Kuswanto Anindya, Weni Antonio Kautsar Apriliani Rahmat Asoka, Shadila Fira Badrunanto Badrunanto Badrunanto, Badrunanto Bambang Prajogo Eko Wardoyo Budi Riza Putra Budi Riza Putra Budi Riza Putra Desi Herliani Dinda Iryawati Dyah Iswantini Era Rahmi Erna Subroto Eti Rohaeti Fauziyatul Munawaroh Fransiska Devy Junardy Hera Nirwati Herdiyanto Herdiyanto Ika Kartika Irma Herawati Suparto Irmanida Batubara Karomah, Alfi Hudatul Kilala Tilaar Latifah K Darusman Latifah K Darusman Latifah K Darusman Pitria Latifah K. Darusman Latifah Kosim Darusman Latifah Kosim Darusman LATIFAH KOSIM DARUSMAN Luthfiana, Widya M. Rafi Mahat, Mohd Muzamir Maily Mustofa Makmum Syafi’i Manggar Arum Aristri Moh Arif Rohman Nur Hakim Mohamad Rafi Mohamad Rafi Mohamad Rafi Monica Ina Martha Muhammad Bachri Amran Muhammad Farid Munawaroh, Fauziyatul Nevianti Zamany Nisa, Ulfiatun Novik Nurhidayat Nur Zamilah Nurdiani Nurdiani Nursifa Mawadah Ramadhanti, Desi Rany Zalvianita Reni Febriani Ria Apriana Ridhova, Aga Rika Indri Astuti Rizna Azrianiningsari Rudi Heryanto Saat Egra Salmi Afifah Selvitia Ulandari Septaningsih, Dewi Anggraini Setyanto Tri Wahyudi Silvia, Riva Silviani, Dea Siti Aisyah SITI SA’DIAH Sri Purwanti Sri Purwanti Sri Purwanti Sukendi, Yeli Suparto, Irma Syarifah Meutiah Eka Sari Syifa Muflihah Tamida Elinda Thaha, Yudi Nugraha Ulfiatun Nisa Utami Dyah Syafitri Waras Nurcholis Widya Luthfiana Wulansari, Laela Yessie Widya Sari Yogo Suro Priyadi

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