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Effects of Gamma Ray Irradiation to Induce Genetic Variability of Teak Planlets (Tectona grandis Linn. F.) Ahmad Parlaongan; Supriyanto; Arum Sekar Wulandari
Journal of Sylva Indonesiana Vol. 5 No. 01 (2022): Journal of Sylva Indonesiana
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (923.103 KB) | DOI: 10.32734/jsi.v5i01.6166

Abstract

Teak planlets (Tectona grandis) of Salomon clones were irradiated by gamma rays to induce genetic variability for growth improvement. The objectives of this research were to analyze the radiosensitivity of T. grandis Salomon clones by gamma ray irradiation and to analyze the genetic variations using Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD). For those purposes, teak planlets were irradiated using gamma rays at 5 different dosages, those were 0 Gy, 10 Gy, 20 Gy, 30 Gy, and 40 Gy. Lethal doses50 (LD50) and reduced doses50 (RD50) were obtained using Curve-fit Analysis. Furthermore, the irradiated planlets were subcultured on medium MS + 0.1 kinetin (M1V0) the surviving M1V0 plantlets were multiplied to MS + 0.1 kinetin, so it is obtained the M1V1 generation. Genetic variation of the mutant was molecularly analyzed using RAPD methods and the variability was calculated using Analysis of Molecular Variance (AMOVA). Results of this research showed that lethal doses50 (LD50) and reduced doses50 (RD50) values were obtained at 24.5 Gy and 7.85 Gy, respectively. It includes genomes of small size and a small number of genomes. The genetic variation of mutant individuals in among and within the treatment of gamma irradiation was 16% and 84% according to AMOVA. It means that the dominant effect of genetic material caused genetic variation in mutant M1V1 generations. This dominance of genetic material could be caused by the response combination of tissue culture treatment and genetic factors that had the potency to be used as materials to select desired clones in the next stage.
Micro Environmental Change in Various Form Land Cover Revegetation Dadan Mulyana; Sri Wilarso Budi R; Basuki Wasis; Arum Sekar Wulandari
Jurnal Manajemen Hutan Tropika Vol. 17 No. 1 (2011)
Publisher : Institut Pertanian Bogor (IPB University)

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Abstract

Evaluation of land rehabilitation (revegetation) activities is necessary for measuring the extent of success of the ongoing activities in rehabilitating and recovering degraded lands. One way for evaluating the success of land rehabilitation (revegetation) is by determining the changes of micro enviroment. The objective of this research was to study the changes of micro environment in various types of revegetated land cover, including scrub/bush land (SB), agricultural land (TP), monoculture teak (JM) and mixed crops (TC) in Ciliwung upper watershed. Research results showed that the highest air temperature and soil temperature were obtained at SB, respectively at 32.8 °C and 26.5 °C, and the lowest at TC, respectively at 28.1 °C and 20.7 °C. Relative humidity and soil moisture were highest at TC (72.3% and 96%) and lowest at SB (60.8%), and the lowest soil moisture occurred at JM (45%). The highest infiltration rate occurred on TP (475.5 mm h-1, very rapid), followed by JM (117 mm h-1, fast) and TC (80 mm h-1), and the lowest at SB (17.65 mm h-1, medium slow). Erosion reductions occurred after 6 years of the revegetation activities with the following results:TC (96,676.1 ton year-1 ha-1), JM (10,790 ton year-1 ha-1), TP and SB (52,867.9 ton year-1 ha-1 and 24,612.6 ton year-1 ha-1). The micro environments for all land cover types were better after revegetation activities.
Uji Perkecambahan Buah Jati Muna dan Jati Malabar Muna Arum Sekar Wulandari
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Germination of teak is often poor. The study on fruits and seeds germination of Java teak, Muna teak, and Malabar  Muna teak was conducted in a series of laboratory and greenhouse experiment. Teak fruits have saveral types of dormancy. So, fruits need pretreatment before they were planted. A recommended pretreatment of teak fruits is to soak the fruits during the night and dry them in the sun during the day.Keyword: teak, Tectoca grandis, fruit germination, seed germination
Teknik Pangkas Akar untuk Meningkatkan Produksi Bibit Melinjo Bermikoriza Arum Sekar Wulandari; . Supriyanto
Jurnal Ilmu Pertanian Indonesia Vol. 18 No. 3 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Mycorrhizal symbiosis is the result of mutualistic interactions between plants and fungi. Melinjo (Gnetum gnemon) naturally associate with ectomycorrhizal Scleroderma sinnamariense and Scleroderma sp. fungi. Artificial inoculation can be applied to young seedlings (1-2 months old), before the roots contain woody material. The purpose of this study was to apply the root pruning techniques to improve gnetum mycorrhizal seedlings production. The study was conducted in a greenhouse using 7 months old seedlings. The treatments consisted of 3 root pruning levels (0, 30, and 50%) and ectomycorrhizal fungi inoculum (control, mycorrhizal seedlings, and soil inoculum). Observations were carried out for 4 months to the seedling growth and root colonization by ectomycorrhizal fungi. Height, diameter, and biomass of melinjo seedlings in all treatments were not significantly different. Thus, root pruning activities do not affect the growth of seedlings. Artificial inoculation produce mycorrhizal seedlings, whereas controls were uninfected. Root pruning increased number of root branching. Root pruning at level 50% increased mycorrhizal seedlings production, but the percentage of root colonization was not significantly difefernt at all pruning levels.
Pengaruh Kombinasi Pemangkasan Akar dan Sumber Inokulum Ektomikoriza Terhadap Pertumbuhan Bibit Melinjo (Gnetum gnemon) Arum Sekar Wulandari; . Supriyanto; Hannum Wulan Febrianingrum
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 3 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.425 KB) | DOI: 10.18343/jipi.20.3.236

Abstract

Root pruning technique may improve new lateral growth. The purposes of this research are to analyse the combined effect of root pruning and inoculum ectomycorrizal types on the growth of gnetum seedling (Gnetum gnemon). This research using split plot design. The source of ectomycorrizhal inoculum as main plot consist of 3 types: control, infected seedling of ectomycorrhizal, and soil inoculum. The root pruning level as sub plot also consist of 3 types: 0, 30, and 50%. Observation is conducted on month 5th and 6th after treatment. The result of this research indicates that root pruning technique on 7 month seedling able to enhance melinjo seedling’s growth and increase ectomycorrhizal colonization (Scleroderma spp.) after 6 months observation. The source of inoculum was able to enhance ectomyrorrhizal colonization but had no significant to gnetum seedling’s growth on month 6th after treatment. Interaction between root pruning 30% and fungal inoculation with soil inoculum source can improve gnetum seedling growth.
KARAKTERISTIK AKAR BEREKTOMIKORIZA PADA Shorea pinanga, Pinus merkusii DAN Gnetum gnemon Melya Riniarti; Irdika Mansur; Arum Sekar Wulandari; Cecep Kusmana
PERENNIAL Vol. 6 No. 1 (2010)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v6i1.192

Abstract

Morphology and anatomy characteristics often used to identify ectomycorrhizal fungi. We used three Scleroderma spp. (Scleroderma columnare, S. dictyosporum), and S. sinnamariense) and inoculated to Shorea pinanga, Pinus merkusii, and Gnetum gnemon. After 6,8, and 10 months, each root tips were collected to determined hyphae colour, branching pattern, clamp-connection, hartig net and mantle. This result revealed that S. sinnamariense did not form association with S. pinanga and P. merkusii but form association with G. gnemon. On the other hand, S. columnare and S. dictyosporum could form association with all the host plants. S. columnare and S. dictyosporum formed white hyphae while S. sinnamariense formed yellow hyphae with monopodial branching pattern. The depth of hartig net and mantle was increased by timed. Key words: ectomycorrhizal fungi, hartig net, mantle, Scleroderma
REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture) Ratna Damayanti Sianturi; Supriyanto Supriyanto; Arum Sekar Wulandari; Benny Subandy
Jurnal Penelitian Hutan Tanaman Vol 14, No 1 (2017): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2838.211 KB) | DOI: 10.20886/jpht.2017.14.1.1-17

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 ABSTRACTembesu (Fagraea fragrans Roxb.) is one of native tree species in peatland forest and has high economical values. Sufficient amount in time of qualified seedlings is needed to support peatland rehabilitation program,and it can be achieved by tissue culture. The objective of the research was to find out the best modificationmedium of MS based on concentration of nitrogen and BAP for induction and multiplication of adventitiousshoot from leaves. The protocol of tissue culture consisted of preparation of plant material, adventitiousshoots induction, shoots multiplication, shoots elongation, rooting and seedling acclimatization. The resultsshowed (1) addition of BAP 1.5 ppm on MS medium (80 mmol N) induced adventitious shoots from leaves;(2) addition of BAP 0.1 ppm on MS medium (80 mmol N) stimulated the highest multiplication of shoots; (3).clone 2 was the best explant on elongation and rooting stage; (4). clone 4 was the best explant inacclimatization stage.Keywords : Fagraea fragrans, tissue culture, medium, organogenesisABSTRAKTembesu (Fagraea fragrans Roxb.) merupakan jenis pohon yang mampu tumbuh di hutan rawa gambut dan bernilai ekonomi tinggi. Untuk mendukung program rehabilitasi lahan gambut, maka diperlukan bibit yangberkualitas, jumlah yang cukup dan tepat waktu. Upaya yang diperlukan dalam menyediakan bibit tersebutadalah perbanyakan bibit melalui teknik kultur jaringan. Tujuan penelitian adalah mendapatkan komposisimedia tumbuh MS dan zat pengatur tumbuh yang tepat dalam pembentukan dan perbanyakan tunas adventifdari daun tembesu. Metode penelitian terdiri dari persiapan bahan tanaman, induksi tunas adventif,perbanyakan tunas adventif, elongasi, pengakaran dan aklimatisasi. Penelitian menunjukkan bahwa hasilyang terbaik yaitu (1) untuk menginduksi tunas adventif diperlukan media MS (80 mmol N) denganpenambahan BAP 1,5 ppm; (2) untuk perbanyakan tunas adventif diperlukan media MS (60 mmol N) denganpenambahan 0,1 ppm BAP; (3) pada tahap elongasi dan pengakaran lebih baik menggunakan tunas adventifdari klon 2; dan (4) pada tahap aklimatisasi lebih baik menggunakan tunas adventif klon 4.Kata kunci : Fagraea fragrans, kultur jaringan, media, organogenesis 
The Effect of Rooting and Acclimatization Media on the Success of Acclimatization of Tembesu (Fagraea fragrans (Roxb.) Miq.) Ganis Citra Purmadewi; Arum Sekar Wulandari; Ratna Uli Damayanti Sianturi
Jurnal Perbenihan Tanaman Hutan Vol 7, No 1 (2019): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/bptpth.2019.7.1.1-12

Abstract

Acclimatization is an attempt to conditioning the plantlets or micro shoots propagated by tissue culture to the environment outside the bottle. This study aims to know the effect of rooting and media acclimatization on the growth of tembesu plantlets in vitro cultures. Two experiments were conducted, these were (1) rooting (in vitro and ex vitro), and (2) type of acclimatization media ( 100% sand, cocopeat + rice husk, sand + cocopeat , sand, rice husk, and sand + cocopeat + rice husk). Based on the data analysis rooting significantly affects the variables growth (percentage of survival and the number of roots) at the acclimatization stage of tembesu.The percentage of planlets survival in the acclimatization are 80percent for in vitro rooting and 75percent for ex vitro rooting, while on stage after acclimatization percentage of survival are 75 percent for in vitro rooting and 67 percent for ex vitrorooting. In vitro rooting have a better growth than ex vitro rooting, but the plants grown from ex vitro rooting are more resistant to stress. The best acclimatization  media for tembesu  is 100 percent of sands.
Growth of Shorea Leprosula Seedlings Prepared in Combo Nursery Technique to Support Mine Reclamation Febrian Ari Nugroho; Irdika Mansur; Arum Sekar Wulandari
Jurnal Wasian Vol 8, No 1 (2021): Jurnal Wasian
Publisher : Balai Penerapan Standar Instrumen Lingkungan Hidup dan Kehutanan (BPPLHK)Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jwas.v8i1.6498

Abstract

Combo nursery technique is a technique where seedlings/cuttings of Shorea leprosula, Gliricidia sepium, and Brachiaria decumbens grass were planted in one polybag to support mining reclamation. To reduce competition effects among the three species, optimum media and size of polybag need to be investigated. The objective of this experiment is to analyse the effect of size of polybag and media composition on the growth of S. leprosula, G. sepium, and B. decumbens grass in the combo nursery technique. The experiment used Randomised Complete Design in Factorial with two factors, i.e. media compositions (soil : compost (2 : 1, v/v), (1 : 1, v/v), (1 : 2, v/v), and soil : compost : rice husk (7:3:1, v/v/v); and size of polybag (15 x 20 cm, 20 x 20 cm, dan 25 x 25 cm). Each treatment had four replications, and each replication consisted of four polybags. The results showed no significant interaction effects between the composition of media and the size of polybag; however, media compositions of soil: compost (1:1) and (1:2), and the sizes of polybag 20 x 20 cm and 25 x 25 cm significantly eliminated competition and significantly increased the growth of S. leprosula and the number of tiller of B. decumbens grass.Keywords: Brachiaria decumbens, Gliricidia sepium, mining reclamation, nursery, Shorea leprosula
Effect of addition of banana stem compost on the growth of jabon (Anthocephalus cadamba Miq.) seedlings growth Arum Sekar Wulandari; Irdika Mansur; Helga Sugiarti
Jurnal Silvikultur Tropika Vol. 2 No. 2 (2011): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.2.2.%p

Abstract

Addition of compost could increase plant growth. Compost derived from banana stem could be added as admixture in nursery medium for growing jabon seedlings.  This research studied the effect of addition of various composts on the growth of jabon  (Anthocephalus cadamba Miq.) seedlings. The objectives of this study were: (1) to test the quality of compost produced from banana stem in improving the growth of jabon seedlings, and  (2) to learn the effect of composts from banana stem and other composts existing in the market (Ofer, Cocopeat, Guano and  Andam) on the growth of jabon seedlings.