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PENGARUH PEMBERIAN VITAMIN E TERHADAP KADAR PROSTAGLANDIN (PGF2α) DAN TNF α PADA PENDERITA DISMENOREA Rishel, Rika Astria; Basyir, Vaulinne
Jurnal Ilmu Keperawatan dan Kebidanan Vol 10, No 1 (2019): JURNAL ILMU KEPERAWATAN DAN KEBIDANAN
Publisher : STIKES Muhammadiyah Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26751/jikk.v10i1.614

Abstract

Pemberian vitamin E pada wanita yang mengalami dismenorea dapat secara langsung menekan kadar ROS dan dapat memperbaiki struktur memberan sel yang rusak. Penelitian ini bertujuan untuk membuktikan pengaruh pemberian vitamin E terhadap kadar Prostaglandin dan TNF-α pada penderita dismenorea. Penelitian ini merupakan  quasi eksperimental dengan desain penelitian Pre andPost Test Only Group Designyang menggunakan 22orang wanita yang mengalami dismenorea primer yang derajat 4-6. Pengambilan sampel dengan simple random sampling. Pemeriksaan kadar prostaglandin dan TNF-α dilakukan di UPTD Balai Laboratorium Kesehatan Provinsi Sumatra barat dengan metode ELISA.Hasil penelitian menunjukkan rerata kadar prostaglandin sebelum pemberian Vitamin E yaitu 205,5 ± 143,3 pg/ml, sedangkan setelah pemberian yaitu 124,8 ± 59,0 pg/ml(p<0,05). Rerata kadar TNF-α sebelum pemberian Vitamin E yaitu 207,2 ± 132,0 ng/l, sedangkan setelah pemberian yaitu 125,9 ± 35,7 ng/ll .Pada analisa data didapatkan kadar prostaglandin dan TNF-α p < 0,05. Kesimpulan penelitian ini ada pengaruh pemberian vitamin E terhadap kadar Prostaglandin dan TNF-α pada penderita dismenorea 
Vitamin D Levels and Risk Factors in Early Onset Preeclampsia, Late Onset Preeclampsia and Normal Pregnancy Basyir, Vaulinne; ayunda, aldina ayunda insani; Andriani, Feni; Suhaila, Afifah
Indonesian Journal of Obstetrics and Gynecology Volume 12 No. 4 October 2024
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v12i4.2122

Abstract

Abstract Objective: To determine the frequency distribution and the relation of risk factors to vitamin D levels in women with early onset (EOSPE), late-onset severe preeclampsia (LOSPE), and normal pregnancy. Methods: This study was cross-sectional with pregnant women diagnosed with EOSPE LOSPE at RSUP DR M Djamil Padang and normal pregnancy at the Pengambiran Health Center. The serum samples of the research subjects were examined for blood levels of Vitamin D using the ELISA kit. Results: Frequency distribution of risk factors for EOSPE respondents aged 20-35 years 50%, mothers not working 85%, single pregnancy 95%, normal blood sugar 80%, and an increase in the number of leukocytes 90%. Frequency distribution of LOSPE respondents, maternal age 20-35 years 60%, mothers not working 95%, single pregnancy 95%, normal blood sugar 65%, and increasing leukocytes 75%. Based on the data analysis test EOSPE respondents with risk factors for preeclampsia history and body mass index had a significant relation with vitamin D levels with P Value 0.00 (P < 0.05). LOSPE mothers with gravid risk factors, history of preeclampsia, history of hypertension, and history of Diabetes Mellitus had a significant relation with Vitamin D levels P Value 0.00 (P < 0.05). Conclusion: The results of statistical tests for LOSPE mothers had a significant relation with Vitamin D levels compared to the risk factors for EOSPE mothers. The incidence of preeclampsia is influenced by complex etiopathogenesis, one of which is influenced by vitamin D levels. Keywords: EOSPE, LOSPE, Elisa-kit, Vitamin D, Etiopathogenesis
Differentiating Maternal Angiogenesis Factors between Early and Late Onset Preeclampsia: Higher sflt-1 in Early Onset Preeclampsia, Lower PlGF and Higher sflt-1/PlGF Ratio in Late Onset Preeclampsia Serudji, Joserizal; Basyir, Vaulinne; Fadhillah, Tara
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.465

Abstract

Background: Early onset preeclampsia (PE) is considered a fetal disorder that is associated with placental dysfunction. While late onset preeclampsia is considered a maternal disorder that associated with a normal placenta. An imbalance of angiogenesis factors, namely soluble Fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF) is not only a strong predictor of PE but can also be a predictor of poor outcomes or adverse complications of PE. This study was conducted to analyze the difference between angiogenesis factor sFlt-1 and PlGF in maternal serum between patients with early and late onset PE. Material and Methods: This was a cross-sectional study involving pregnant women with PE who were ≥18 years old and gestational age >20 weeks had singleton pregnancies. Subjects who had a major morphological abnormality of a fetus diagnosed with USG and chromosomal abnormality of the fetus were be excluded. Systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) were measured using a digital blood pressure measuring instrument. sFlt-1 and PlGF levels were measured using enzyme linked immunosorbent assay (ELISA). Result: This study showed the level of maternal serum sFlt-1 was 7522.95 pg/mL and the level of maternal serum PlGF was 222.25 pg/mL. There was a difference between sflt-1, PlGF, and the sflt-1/PlGF ratio (p=0.00; p=0.00; p=0.00) in early and late onset of PE where at early onset PE was found higher sflt-1 and late-onset PE had lower PlGF and higher sflt-1/PlGF ratio. Conclusion: There are differences in sFlt-1, PlGF, and sFlt-1/PlGF in early and late onset PE, as higher sflt-1 is found in early onset preeclampsia, while lower PlGF and higher sflt-1/PlGF ratio are found in late onset preeclampsia.Keywords: early onset, late onset, preeclampsia, maternal angiogenesis factor, Flt-1, PlGF
Pengaruh Likopen terhadap Kadar Soluble FMS-Like Tyrosine Kinase 1 pada Preeklamsi Basyir, Vaulinne; Fauziah, Prima Nanda; Pramatirta, Akhmad Yogi; Yanwirasti, Yanwirasti; Mose, Johanes C.; Oenzil, Fadil
Jurnal Kesehatan Andalas Vol. 9 No. 4 (2020): Online December 2020
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1513

Abstract

Preeklamsi merupakan penyebab utama morbiditas dan mortalitas baik maternal maupun perinatal. Tujuan: Menganalisis pengaruh pemberian likopen terhadap kadar sFlt-1 secara in vitro pada sel trofoblas model preeklamsi. Metode: Penelitian ini dilakukan secara eksperimental di Laboratorium Genetika Molekuler dan Kultur Sel Fakultas Kedokteran Universitas Padjadjaran selama periode 2017-2018. Sampel serum diperoleh dari RS. Dr. Hasan Sadikin Bandung, sesuai kriteria inklusi dan eksklusi. Kriteria inklusi adalah pasien yang telah didiagnosis preeklamsi berdasarkan pemeriksaan tekanan darah dan protein urin, dan kriteria eksklusi adalag pasien yang tidak datang kontrol atau tidak bisa dihubungi. Penelitian ini terdiri dari dua tahap, pertama ditentukan nilai LD50 likopen terhadap sel trofoblas kemudian dijadikan dasar nilai konsentrasi yang akan digunakan, selanjutnya dilakukan pemeriksaan sFlt-1 menggunakan metode Elisa pada kultur sel trofoblas kondisi normal dan preeklamsi dengan analisa data menggunakan analisis varians (Anova) dan uji Duncan untuk mengetahui perbedaan antar variabel. Hasil: Nilai LD50 adalah 250 ug/ml dan serial konsentrasi yang digunakan mulai dari 1,953 sampai dengan 125 ug/ml. Didapatkan kadar sFlt-1 menurun secara signifikan (p0,05) dari 39,5445 menjadi 31,1342 pg/ml pada sel trofoblas yang diinduksi dengan serum preeklamsi setelah pemberian konsentrasi likopen sebesar 15,625-31,25 ug/ml yang nilainya mendekati keadaan pada sel trofoblas yang diinduksi serum kehamilan normal. Pemberian likopen dapat menurunkan kadar sFlt-1 pada sel trofoblas preeklamsi. Simpulan: Likopen dapat merubah kadar sFlt-1 dan perubahannya dipengaruhi oleh konsentrasi dan jenis serum.Kata kunci: likopen, preeklampsi, sFlt-1 
The Relationship between Family Support and Maternal Readiness with Anxiety Levels Before Delivery Ritonga, Dewi; Basyir, Vaulinne; Rasyid, Rosfita
JURNAL KEBIDANAN Vol 15, No 1 (2025): April 2025
Publisher : Politeknik Kesehatan Kementerian Kesehatan Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkb.v15i1.12610

Abstract

Anxiety in pregnant women is a common emotional condition that occurs during pregnancy, characterized by excessive feelings of anxiety, worry, or fear. Pregnancy anxiety itself is experienced by many pregnant women due to lack of knowledge, readiness and support from family, community and environment. Excessive anxiety during pregnancy can lead to an increased incidence of uterine atony, infection, maternal fatigue, and shock, while in infants it can increase the risk of preterm birth and low birth weight (LBW). This study aims to examine the relationship between family support and maternal readiness with the anxiety level of pregnant women before delivery. This study used a quantitative research design with a cross-sectional approach, conducted in the working area of Andalas Padang Community Health Center. The sample consisted of 59 third-trimester pregnant women, selected through Simple Random sampling. Data collection was carried out using a questionnaire. The results of this study showed that the percentage of pregnant women who received family support and experienced less severe anxiety was 52.2% (p=0.048), including analysis of this data using the Chi-Square Test. Furthermore, it was found that pregnant women who were less prepared for childbirth experienced severe anxiety at a rate of 50.0% (p=0.002). In conclusion, there is a relationship between family support and maternal readiness with anxiety levels before delivery in the working area of Andalas Padang Community Health Center.
ANALISIS KUALITAS PELAYANAN ANTENATAL CARE TERPADU PADA IBU HAMIL DI PUSKESMAS AMBACANG KECAMATAN KURANJI KOTA PADANG TAHUN 2024 Muttakia Hardy, Siti Khalisha; Yetti, Husna; Basyir, Vaulinne
AN-NUR: Jurnal Kajian dan Pengembangan Kesehatan Masyarakat Vol. 5 No. 2 (2024): Annur: Jurnal Kajian dan Pengembangan Kesehatan Masyarakat
Publisher : Fakultas Kesehatan Masyarakat Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/annur.5.2.121-132

Abstract

Antenatal care (ANC) merupakan pelayanan untuk ibu hamil dalam upaya menjaga kesehatan ibu dan bayi dan mengurangi risiko komplikasi saat kehamilan dan persalinan dengan mendeteksi dini masalah dan risiko tinggi. Pada tahun 2022, cakupan K1 dan K4 ANC Puskesmas Ambacang menempati posisi yang cukup tinggi, namun terdapat tiga kematian ibu di wilayah kerja Puskesmas Ambacang. Efektivitas dan kualitas ANC di tidak hanya berdasarkan jumlah kunjungan, tetapi juga berbagai aspek, yaitu input, proses, dan output, serta kepuasan ibu hamil. Penelitian ini bertujuan untuk melihat gambaran pelaksanaan pelayanan ANC di Puskesmas Ambacang dengan meninjau aspek input, proses, output, dan kepuasan. Penelitian ini menggunakan metode kualitatif dengan perspektif studi kasus dan menghasilkan data deskriptif berupa gambaran dan kata-kata tertulis dari wawancara mendalam terhadap informan serta perilaku yang diobservasi, juga telaah dokumen. Informan yang diwawancara pada penelitian ini sebanyak 12 orang. Komponen input sudah sesuai standar dengan sumber daya manusia yang memadai, baik dari kualitas dan kuantitasnya. Dana pelayanan dari BOK, APBD, dan BLUD. Sarana dan prasarana sudah layak, namun terdapat kendala pada luas ruangannya. Terdapat SOP pelayanan yang mengacu pada kebijakan dan standar pelayanan minimal. Komponen proses sudah berjalan dengan baik dan melaksanakan pelayanan 10T, namun pencatatan kunjungan pelayanan belum maksimal. Komponen output berupa angka cakupan K1–K4 yang mengalami penurunan dan berada dibawah target nasional. Secara garis besar, ibu hamil sudah merasa sangat puas dengan aspek SERVQUAL pelayanan yang diberikan. Secara keseluruhan, pelayanan ANC di Puskesmas Ambacang berjalan dengan baik, namun perlu mengoptimalkan proses pencatatan angka kunjungan untuk meningkatkan kualitas pelayanan. --- Antenatal care (ANC) is a service provided to pregnant women to maintain the health of both the mother and the baby, and to reduce the risk of complications during pregnancy and childbirth by early detection of problems and high-risk factors. In 2022, the coverage of K1 and K4 ANC at Puskesmas Ambacang was relatively high, although there were three maternal deaths within its service area. The effectiveness and quality of ANC are not solely measured by the number of visits but also by various aspects, including input, process, output, and maternal satisfaction. This study aims to assess the implementation of ANC services at Puskesmas Ambacang by reviewing these aspects. The research adopts a qualitative approach with a case study perspective, producing descriptive data from in-depth interviews with 12 informants, observations, and document reviews. The input components meet the required standards, with adequate human resources in terms of both quality and quantity. Service funding is sourced from BOK, APBD, and BLUD. The facilities and infrastructure are adequate, though there are challenges related to the space available. Standard Operating Procedures (SOPs) are in place, aligned with policies and minimum service standards. The process component is functioning well, with the implementation of 10T services, although documentation of service visits has not been fully optimized. The output component, which refers to K1-K4 coverage, has declined and is below the national target. Overall, pregnant women express a high level of satisfaction with the SERVQUAL aspects of the services provided. In conclusion, ANC services at Puskesmas Ambacang are generally functioning well, but improvements are needed in optimizing the documentation and reporting of visit data to enhance service quality.
Asuhan Kebidanan Nifas Pada Ny.”A” Post Op Sc Indikasi Fetal Hipoksida Dengan Absent End Diastolic Dan Intrauterine Growth Retriction Anggraini, Fitri; Basyir, Vaulinne; Karmia, Hudila Rifa
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.11724

Abstract

Angka Kematian Ibu (AKI) merupakan salah satu indikator penting dalam menilai derajat kesehatan masyarakat dan keberhasilan pembangunan sektor kesehatan. Salah satu penyebab utama tingginya AKI adalah perdarahan postpartum, yang banyak terjadi pada masa nifas—periode krusial setelah melahirkan. Seksio sesarea (SC) merupakan metode persalinan yang sering digunakan untuk menangani kasus kehamilan risiko tinggi, termasuk fetal distress dan Intrauterine Growth Restriction (IUGR), yang keduanya merupakan kondisi serius yang berdampak pada kesehatan ibu dan bayi. Studi ini bertujuan untuk mengkaji asuhan kebidanan masa nifas pada Ny. “A” pasca SC dengan indikasi fetal hipoksia dan IUGR di Rumah Sakit Universitas Andalas Padang pada tahun 2025. Pendekatan yang digunakan adalah studi kasus dengan fokus pada identifikasi masalah, penatalaksanaan, serta evaluasi asuhan kebidanan yang diberikan selama masa nifas. Hasil kajian menunjukkan bahwa penatalaksanaan yang tepat dan holistik pada masa nifas, termasuk pemantauan kondisi fisik dan psikologis ibu, mampu mencegah komplikasi lanjutan dan meningkatkan pemulihan pascapersalinan. Peran bidan sangat penting dalam memberikan pelayanan profesional, beretika, dan berbasis kompetensi, terutama dalam masa kritis 24 jam pertama pascapersalinan. Kajian ini menekankan pentingnya optimalisasi asuhan kebidanan masa nifas untuk menurunkan AKI dan meningkatkan kualitas hidup ibu pascapersalinan
Hubungan Infeksi Gardnerella vaginalis Dengan Manifestasi Klinis Keputian Penyebab Vaginosis Bakterialis Pada Ibu Hamil Ketuban Pecah Dini Di RSUP Dr. M. Djamil Padang Anggraini, Fitri; Putra, Andani Eka; Basyir, Vaulinne
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 2 (2025): September 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i2.11911

Abstract

Premature rupture of membrane (PROM) is one of the abnormalities found in pregnancy. The results find out that Lactobacillus is associated with the reduced risk of premature rupture of membrane (PROM), while Gardnerella, Prevotella, Megasphaera, Ureaplasma and Dialister are associated with the increase risk of PROM. Gardnerella vaginalis, a facultative anaerobic bacterium, is the most common microbe associated with bacterial vaginosis (BV).The research used cross sectional research design with retrospective data collection method. The research included all vaginal swab samples from pregnant women with premature rupture of membranes at RSUP Dr. M. Djamil, Padang. The sampling technique used was consecutive sampling, with 31 samples from pregnant women who experienced premature rupture of membranes, complemented with data from electronic/manual medical records. Clinical data collected included complaints such as vaginal discharge. The microbes that cause bacterial vaginosis were examined using Real-Time PCR at the PDRPI Laboratory, Faculty of Medicine, Universitas Andalas. Data then were analyzed using the chi-square test, followed by calculation of the Odds Ratio (OR).he results showed no significant association between the presence of G. Vaginalis and vaginal discharge (p = 0.384; OR = 2.67), Based on the analysis, most bacterial vaginosis infections occurred without the evident of clinical manifestations (asymptomatic) which caused to delayed diagnosis and an increased likelihood of cases being inadequately managed.The conclusion of this research shows that there is no significant association between microbes of bacterial vaginosis and manifestation of vaginal discharge in pregnant women. Antenatal care (ANC) interventions should include the development of a routine vaginal infection screening system within ANC services. Keywords: Gardnerella vaginalis, Bacterial Vaginosis, Pregnant Women
DIFFERENCES IN PROTEASE ACTIVATED RECEPTOR-1 AND THROMBINE LEVELS IN PREECLAMPSIA AND NORMAL PREGNANCY Khatimah, Gistin Khusnul; Serudji, Joserizal; Basyir, Vaulinne
Andalas Obstetrics And Gynecology Journal Vol. 6 No. 1 (2022)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.6.1.27-34.2022

Abstract

Introduction: Preeclampsia is a condition caused by alterations in endothelial function during pregnancy. Changes in endothelial function result in an increase in coagulation and microvascular fibrin accumulation, which results in impaired placental perfusion. Thrombin, which converts fibrin to fibrinogen, as well as platelet activity, the fibrinolytic system, and anticoagulants, are all procoagulant circumstances in preeclampsia. Thrombin contributes to the pathogenesis of preeclampsia by increasing the expression of sFlt-1 thereby providing an antiangiogenic response. Protease Activated Receptor-1 (PAR-1) is a mediator of thrombin for coagulation and inflammation in preeclampsia. Inhibition of Protease Activated Receptor-1 expression in trophoblasts can enhance placental angiogenesis and vascular remodeling. Recently, only few studies have assessed the levels of Protease Activated Receptor -7 and thrombin in preeclampsia.Objective: To determine the difference in levels of Protease Activated Receptor-1 and thrombin in preeclampsia and normal pregnancyMethods: This study is observational with a cross-sectional comparative study design. Sampling was conducted from March 2020 to March 2021. A total of 66 patients were investigated, with 33 samples of preeclampsia and 33 samples of normal pregnancy. The independent sample T-test was used for statistical analysis.Results: The mean levels of Protease Activated Receptor-1 in the preeclampsia group were higher at 28.56 ± 7.68 ng/mL while normal pregnancy was 21.67 ± 6.92 ng/mL. The results of statistical tests showed that there was a significant difference in levels of Protease Activated Receptor-1 between the preeclampsia and normal pregnancy groups (p<0.05). The mean thrombin level in the preeclampsia group was higher at 72.23 ± 7.99 ng/mL, while in normal pregnancy it was 63.70 ± 8.92 ng/mL. The difference in thrombin levels between the preeclampsia and normal pregnancy groups was statistically significant (p<0.05).Conclusion: Preeclampsia was associated with greater levels of Protease Activated Receptor-1 and thrombin than normal pregnancy. There was a significant difference in the mean levels of Protease Activated Receptor-1 and thrombin between preeclampsia and normal pregnancy.Keywords: Thrombin, Protease Activated Receptor-1(PAR-1), Preeclampsia
The Relationship Between Macronutrient Intake and Stunting Among Children Aged 1–2 Years: Quantitative and Qualitative Approaches Nesva, Intan Silvia; Sulastri, Delmi; Basyir, Vaulinne
Journal of Epidemiology and Public Health Vol. 10 No. 4 (2025)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2025.10.04.04

Abstract

Background: Stunting among children aged 1–2 years remains a critical public health concern in Indonesia, particularly during the vital 1,000-day window of child development. This study aimed to analyze the relationship between macronutrient intake and stunting using a mixed-methods approach. Subjects and Method: This was a sequential explanatory mixed-methods study conducted from February 2024 to May 2025 at Community Health Center Dadok Tunggul Hitam, Padang City. The quantitative component employed a case-control design involving 66 children aged 1–2 years, divided equally into stunted (cases) and non-stunted (controls) groups. Sampling was conducted purposively for cases and by simple matching for controls. The independent variables were intake of carbohydrates, proteins, and fats; the dependent variable was stunting, defined using WHO growth standards. Macronutrient intake was assessed via a 24-hour recall. Data were analyzed using Chi-square tests to determine associations, and Odds Ratios (OR) with 95% Confidence Intervals (CI) were reported. The qualitative phase included interviews with mothers, health workers, and community volunteers, analyzed thematically using NVivo 12. Results: Children with inadequate carbohydrate intake were significantly more likely to be stunted (OR=3.50; 95% CI=1.27 to 9.64; p=0.015). No statistically significant associations were found for protein intake (OR=2.60; 95% CI=0.91 to 7.44; p=0.074) or fat intake (OR=2.44; 95% CI=0.89 to 6.70; p=0.082). Qualitative findings revealed barriers such as loss of appetite during illness, preference for snacks, food aversions, and limited maternal knowledge and resources, all of which contributed to inadequate macronutrient intake. Conclusion: Inadequate carbohydrate intake was significantly associated with stunting in children aged 1–2 years. Contextual factors such as eating behavior and parental feeding practices further influenced nutritional outcomes. Interventions should address both dietary intake and behavioral barriers within families.