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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Farmasi Udayana Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Program Kreativitas Mahasiswa - Penelitian JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Syntax Literate: Jurnal Ilmiah Indonesia JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) SANITAS: Jurnal Teknologi dan Seni Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of Health Sciences and Medicine JOURNAL SCIENTIFIC OF MANDALIKA (JSM) Journal Sport Science, Healt and Tourism of Mandalika (Jontak) Indonesian Journal of Perinatology Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Journal Pharmaceutical Science and Application (JPSA)

Ketut Widyani Astuti

Program Studi Farmasi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 4222729

Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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UJI KONFIRMASI DAN PENETAPAN KADAR MORFIN DENGAN KLT-SPEKTROFOTODENSITOMETRI I Made Agus Gelgel Wirasuta; Ida Ayu Shanti Primaningrum; Ketut Widyani Astuti
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 4 (2014): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Screening and determination test of morphine using TLC-Spectrophotodensitometry method have been studied. Screening test based on the hRfc – value and the correlation value between detected spectrum and reference. The analysis results were improved by using the shift of spectrum reaction. The maximal result of screening test was obtained when determinate the hRfc-value with error window of three and the spectrum correlation above 0.90. The reaction of spectrum shift showed that morphine spectrum was not significantly changed after plates sprayed with HCL 10% and KOH 0.1 M so that could be used to increasing the analysis result of substance identity determination. Validation of method showed precision of method was 1.69%, the limit of detection 93.1 ng/spot and the limit of quantitation was 310,3 ng, percentage recovery of morphine from plasma was 106.34 ng ±10.79 (10.1%). TLC Spectrophotodensitometry could be used as simple and cheap method for screening and determination test of the morphine.

Optimasi Waktu Maserasi untuk Manggis (Garcinia mangostana L.) Rind Menggunakan Pelarut Etil Asetat Ni Putu Ayu Dewi Wijayanti; Dewi LPMK; Astuti KW; Fitri NPE
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 3 No. 1 (2016): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Universitas Airlangga

Background: Maceration is a non-heating extraction method the result of which is affected by the type of solvent and the maceration time. Objective: To get the higher alpha-mangostin concentration (Garcinia mangostana L.) rind that was macerated using ethyl acetate solvent and to determine the optimum maceration time needed to produce high concentration of alpha-mangostin. Methods: the mangosteen rind was macerated using ethyl acetate with the variation of the maceration time of 6, 12, 24 and 48 hours. The extract was then dried using the freeze drying method. The optimum maceration time was determined by looking at the highest concentration of alpha-mangostin from each extraction time using TLC-densitometry method with a silica gel stationary phase of GF254 and a mobile phase of chloroform”methanol (10:0.1, v/v). Results: The results showed that the difference in the maceration time can affect the concentration of alpha-mangostin compound contained in the extract. Conclusions: It was found out that the 24-hour maceration time produced the highest concentration of alpha-mangostin that is 3031.34 ng.

ACETOSAL, BUAH MENGKUDU (MORINDA CITRIFOLIA L.) DAN WAKTU PERDARAHAN I Wayan Putu Sutirta Yasa; Ketut Widyani Astuti; I Gusti Made Aman
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1007

Research regardless the effect of noni fruit for increasing bleeding time have already been carried out widely. The similar activity ofnoni fruit extract and acetosal can be concerned that the fruit extract has a potential activity for prolonged bleeding time. This study aimsto know the present of prolonged of bleeding time as a results of intake of combination of noni fruit extract with acetosal on mice. Thisresearch was carried out at Unit Binatang Percobaan, Departemen Farmakologi Universitas Udayana. This is an experimental study withpre and post-test control group design. Subject was compromised of 3 groups of mice and each group contain of 7 mice. The first groupwas treated with a dose of 40 mg/kg bw acetosal, the second group treated with a dose of 100 mg/kg bw ethanol noni fruit extract, andthe third group treated with combination of 40 mg/kg bw acetosal and 100 mg/kg bw ethanol extract of noni fruit. All groups werefed once per day for a week. Bleeding time was determined on the basis of tail bleeding method. This study results that the first groupexperience bleeding time increased from 61.42±9.43 second to 160.71±19.77 second. Increase bleeding time of the second group is from59.14±7.12 to 138.14±59.91 second. For the third group, the bleeding time increases from 65.00±7.91 to 213.00±20.92 second.One Way ANOVA analysis indicates that there is a significant different among these three groups after treatment p = 0.006 (p<0.05).Bleeding time of the third group which was treated with combination of noni fruit and acetosal results in the highest increase compareto the other two groups. In conclusions, combination of noni fruit and acetosal treatment results in increase of bleeding time on mice.

Validation of Method for Determining Alpha- Mangostin Level of Ethyl Acetate Extract of Mangosteen Rind Using TLCSpectrodensitometry N. P. A. D. Wijayanti; L.P.M.K. Dewi; K.W. Astuti
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Abstract Objective: Mangosteen rind (Garcinia mangostana L.) contains secondary metabolites, namely alpha-mangostin which has antioxidant activity, as well as antibacterial and anti-aging properties. In order to obtain a maximum amount of alpha-mangostin compounds, the maceration method using ethyl acetate was used. To ensure the effectiveness of the mangosteen rind extraction process, all of the processes and methods in preparing the extract should be properly controlled, particularly the analytical method used in determining the alpha-mangostin level of the extract. The method used should be able to determine the alphamangosteen level accurately. This study aimed to test the validation of the analytical method used. Method: The parameters for the validation of the analytical method tested in this study include accuracy, precision, range and linearity, limit of detection (LOD), limit of quantitation (LOQ) and specificity. In this study, the level of alpha-mangostin in the extract was determined using TLC-Spectrodensitometry with the stationary phase of the silica gel plate GF 254 and the mobile phase of chloroform and methanol (10:0.1 v/v). Result: The results of the study showed that this method has met the acceptance criteria for validation with the accuracy value in the range of percent recovery, from 93.85 % to 111.16%; precision with KV <2%; specification with spectrum correlation >0.99; linearity with r =0,99372; limit of detection (LOD) of 5.33 ng and limit of quantitation (LOQ) of 11.43 ng.

DEVELOPMENT OF GEL DOSAGE FORM OF ETHYL ACETATE EXTRACT OF MANGOSTEEN RIND Ketut Widyani Astuti; Ni Putu Ayu Dewi Wijayanti; I Gusti Ngurah Jemmy Anton Prasetia
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Abstract Alpha-mangostin is a xanthonoid compound contained in mangosteen rind (Garcinia mangostana L.) and has an antibacterial effect against Staphylococcus aureus, the bacterium that causes acne. In this study, mangosteen rind extract is formulated in a gel dosage form. This study aimed to determine the optimum formula, the physical and chemical properties as well as the optimum formula release profile of the gel dosage form of mangosteen rind extract. The mangosteen rind extract gel formula consists of viscolam, propylene glycol, glycerin, microcare®, ethyl acetate extract of mangosteen rind, and distilled water. The formula was optimized by varying the concentrations of viscolam (2% and 5%), propylene glycol (5% and 20%) and glycerin (2% and 15%) using a factorial experimental design program called Design Expert 7.0.0. The 8 formulas were developed into gel dosage form and the physical and chemical properties were then evaluated. The evaluation tests include viscosity, dispersive power, and pH tests. The evaluation results were processed using the Design Expert 7.0.0 program to determine the optimum formula. The results of the analysis showed the optimum formula of gel dosage form of mangosteen rind extract with viscolam percentage of 4.97%, propylene glycol of 9.91%, glycerin of 12.23%, microcare® of 0.3%, TEA (q.s), ethyl acetate extract of mangosteen rind of 1%, and distilled water of 45%. The evaluation results of the physical and chemical properties of the gel optimum formula revealed the viscosity of 2,345 cps, dispersive power of 6.59 cm, and pH of 6.74. Alpha-mangostin release test on the optimum formula revealed a flux value of 41.327 ?g/cm2/t1/2. From these results, it is concluded that the optimum formula has met the physical and chemical characteristics of a good gel.

Optimization Of Isolation Method Of Carrageenan From Kappaphycuss Alvarezii Doty Using Factorial Experimental Design Ketut Widyani Astuti; Ni Putu Ayu Dewi Wijayanti; I Gusti Ngurah Agung Dewantara Putra; Ni Putu Linda Laksmiani
Journal of Health Sciences and Medicine Vol 1 No 2 (2017): JHSM (September 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.941 KB)

Kappaphycus alvarezii Doty is a species of red algae ( Rhodophyceae) producing kappa carrageenan. The value of carrageenan will increase if it is able to form thermoreversible gels. The quality of carrageenan is determined by the isolation method which includes several stages, namely soaking, extraction, purification, and drying. This study aims to obtain an optimum isolation method of carrageenan from seaweed Kappaphycus alvarezii Doty using the factorial experimental design software, Design Expert Version.7.0.0. with the characteristics that meet the standards set by FAO. The seaweed was collected from the sea in Nusa Lembongan, Bali Province. The carrageenan isolation method was optimized by varying three factors, namely the concentration of NaOH (4% and 8%), extraction time (0.5 hour and 3 hours), and precipitation time (0.25 hour and 0.5 hour) in order to obtain 8 formulae which then went through an evaluation of the physical and chemical characteristics of the carrageenan. The physical and chemical characteristics include the yield, viscosity, sulfate content, and ash content, analyzed using Design Expert Version 7.0.0 program to determine the optimum method of isolation. The analysis showed that the optimum method of carrageenan isolation was using NaOH with a concentration of 4.40%, the extraction time of 2.16 hours and the precipitation time of 0.29 hour. The physical and chemical characteristics of carrageenan with the optimum isolation method include the yield of 25.73%, the viscosity of 11.92 cPs, sulfate content of 31.95% and the ash content of 26.16% which have met the standards set by FAO.

Pengembangan Kuisioner Udayana untuk Penilaian Kepuasan Pasien terhadap Pelayanan Kefarmasian oleh Apoteker di Pusat Kesehatan Masyarakat Luh Putu Febryana Larasanty; Made Krisna Adi Jaya; Ketut Widyani Astuti; I Wayan Martadi Santika
JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Vol 11, No 1
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jmpf.60998

The implementation of pharmaceutical care by pharmacists at the community health center has been regulated in the Minister of Health Regulation No. 74 of 2016. Based on that regulation, assessment of pharmaceutical care services is needed to ensure the quality of services provided by pharmacists. This study aims to develop an Udayana questionnaire that can be used to evaluate the quality of pharmaceutical care provided by pharmacists at community health centers. This research is quantitative (survey implementation) carried out in 2 phases. The first phase is the design of the Udayana questionnaire. The second phase is to verify the validity and reliability of the questionnaire. The questionnaire validity test was carried out in three stages, namely the logical validity test, content validity, and construct validity. The questionnaire statement develop based on Minister of Health Regulation No. 74 of 2016 obtained 31 statements which are divided into 5 dimensions of service quality. The logical validity test resulted in 30 statements that were declared valid by experts. Content validity test is done by calculating the value of the content validity ratio (CVR) and the value of the content validity index (CVI). The CVR and CVI results showed that 7 statements were eliminated because they were not valid (CVR value <0.99 and CVI <70%). Construct validity test and questionnaire reliability were tested on 384 respondents who had met the inclusion criteria. The construct validity test resulted 23 valid statement (r = 0.148). Results of the reliability test based on Cronbach's Alpha value is greater than 0.6, indicating that all the valid statement items in the Udayana questionnaire are reliable. Based on the results of validity and reliability tests, 23 statements of Udayana's questionnaire are stated valid and reliable and can be used to evaluate the quality of pharmaceutical care at the community health center.

Torsion of ovarian cyst in the third trimester of pregnancy: case report I Nyoman Hariyasa Sanjaya; Cokorda Istri Mirayani Pemayun; Ni Putu Nining Gianni; Made Diah Vendita Sakuntari; Ni Wayan Dewi Purwanti; Ni Luh Made Diah Mas Cahyani Putri; Ni Luh Md Dwi Laxmi Satriani; Ni Komang Anik Pirgantari; Firsta Sesarina Mintariani; Anak Agung Wahyu Putri Agustini; Ketut Widyani Astuti
Indonesian Journal of Perinatology Vol. 3 No. 1 (2022): Available online : 1 June 2022
Publisher : The Indonesian Society of Perinatology, South Jakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/inajperinatol.v3i1.17

Introduction: The entire or partial rotation of the adnexa around its vascular axis or pedicle is known as ovarian torsion. Predisposing factors include short length, unrestricted motion, and a long pedicle. It’s tough to pinpoint the exact cause. Dermoid and serous cyst adenomas are the most common. The complete torsion blocks the venous and lymphatic systems, resulting in stasis, venous congestion, bleeding, and necrosis. The cyst becomes irritable and may explode. A soft cystic mass separate from the uterus can be seen on examination of the affected person, who usually complains of abrupt severe discomfort in the stomach and pelvis. At some time during pregnancy, the risk of ovarian torsion increases by a factor of five. The incidence rate is five per 10,000 pregnancies. Torsion of ovarian tumors was more common among women in their reproductive years. The majority of the instances were seen in pregnant women (22.7%) rather than non-pregnant women (6.1%). Case Description: In this case, we report a 27-year-old primigravida with 36 weeks and 5 days of pregnancy. She presented to the hospital with acute pain abdomen in the lower right (lumbar). She has a history of pregnancy control at a private clinic and there are no abnormalities in her pregnancy. She was hospitalized at 35 weeks 3 days of pregnancy due to the same complaint and has collaborated with the surgeon. No abnormalities were found on the abdominal ultrasound and urology at that time. The baby was born at 36 weeks 6 days of gestation by cesarean section, a twisted ovarian cyst was found on the right ovary, a dextra salpingectomy was performed for oophorectomy and an appendicectomy was also performed at the same time in collaboration with the surgeon. A healthy baby with a weight of 2300 grams and full of breastfeeding. Conclusion: This example demonstrates the problem of manufacturing desirable excellent radiological ultrasound imaging of the pelvic organs in late pregnancy. ultrasound exam in early pregnancy has to also be geared toward the cervix and adnexa which ends up in early analysis and management of ovarian hundreds, thereby fending off future emergency conditions and possible preterm transport.

Twin reversed arterial perfusion syndrome (TRAP or acardiac twin) in pregnancy: a case report I Nyoman Hariyasa Sanjaya; Cokorda Istri Mirayani Pemayun; Ni Luh Made Diah Mas Cahyani Putri; Ni Wayan Dewi Purwanti; Made Diah Vendita Sakuntari; Ni Putu Nining Gianni; Ni Komang Anik Pirgantari; Ni Luh Md Dwi Laxmi Satriani; Firsta Sesarina Mintariani; Anak Agung Wahyu Putri Agustini; Ketut Widyani Astuti
Indonesian Journal of Perinatology Vol. 3 No. 1 (2022): Available online : 1 June 2022
Publisher : The Indonesian Society of Perinatology, South Jakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/inajperinatol.v3i1.18

Introduction: A type of pregnancy that occurs in monochorionic twin pregnancies which results in the coexistence of normal "pump" twins and cardiac twins is called Twin Reverse Arterial Perfusion Syndrome (TRAP). The low oxygen pressure going to the baby causes many unique changes in the twins' physiology. These changes can lead to high prenatal mortality. Case Presentation: We present the case of a 25-year-old woman, primigravida, whose obstetrician referred to Kasih Medika Bali with suspicion of a large placenta. However, upon arrival at 35 weeks and 2 days of gestation, an ultrasound showed a twin pregnancy with suspicion of a TRAP pregnancy. Then, a baby girl was born at Payangan Hospital, Gianyar, Bali by Sectio Caesarea with a birth weight of 3155 grams, an Apgar score of 6-8-9, and a placental weight of 815 grams. The results of the examination of the second fetus that did not develop, weighing 1,705 grams in the placenta did not have an attached umbilical cord. The doctor's suspicions about the TRAP pregnancy were indeed true according to the conditions at the time of the twins' birth. The doctor found 1 healthy baby and 1 other like mass with a size large enough on ultrasound at 35 weeks 2 days of gestation. Conclusion: The incidence of pregnancy like this is very rare and needs further study in order to detect early abnormalities that occur in pregnancy. Thus, we can be aware of the risks for both mother and fetus and even treat them early. Couples can be counseled optimistically that the recurrence rate of TRAP syndrome tends to be low so that they can better plan future pregnancies in all aspects.

Planned home birth in low-risk pregnancies Made Diah Vendita Sakuntari; I Nyoman Hariyasa Sanjaya; Cokorda Istri Mirayani Pemayun; Ni Wayan Dewi Purwanti; Ni Putu Nining Gianni; Ni Luh Made Diah Mas Cahyani Putri; Ni Komang Anik Pirgantari; Ni Luh Md Dwi Laxmi Satriani; Firsta Sesarina Mintariani; Anak Agung Wahyu Putri Agustini; Ketut Widyani Astuti
Indonesian Journal of Perinatology Vol. 3 No. 1 (2022): Available online : 1 June 2022
Publisher : The Indonesian Society of Perinatology, South Jakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/inajperinatol.v3i1.19

Introduction: Planned home births are often a safe option for low-risk pregnancies, according to prior studies. This study aims to ascertain the characteristics of planned home deliveries with skilled medical staff in low-risk pregnancies as well as the outcomes on mother and infant health in Denpasar, Bali. Methods: The planned home births from 2010 to 2019 were examined in this study using a retrospective descriptive design. The study included all intended home deliveries, including those that required hospital transfers owing to difficulties. As many as 168 planned home births with private doctor assistance made up the study’s sample. Sociodemographic and clinical variables were both examined in this analysis. Results: The mean age of the sample in this study was 32 years. The findings indicate that women planning a home delivery with a licensed doctor in low-risk pregnancies have a better likelihood of spontaneous vaginal birth and favorable outcomes for maternal health. Additionally, there is a reduced likelihood of hospital transfer (10%). Furthermore, evidence demonstrates that planned home births are typically linked to fetal wellbeing. Conclusion: The majority of women gave birth between 38 and 40 weeks gestation. The majority of samples were deliveries were normal vaginal births. The main reasons for transfer were due to the arrested cervical dilation or the arrested progress of the fetal head.

Co-Authors Anak Agung Gede Rai Yadnya Putra Anak Agung Wahyu Putri Agustini Anak Agung Wahyu Putri Agustini Anak Agung Wahyu Putri Agustini Arifani Siswidiasari Aryadnyani, Ni Putu Aryani, Kadek Indra Cokorda Istri Mirayani Pemayun Cokorda Istri Mirayani Pemayun Cokorda Istri Mirayani Pemayun D. A. Satriawati Damayanti, Dewa Ayu Trisna Dewa Ayu Satriawati Dewi Inderiati Dewi LPMK Dewi, Ni Pande Kadek Sinta Firsta Sesarina Mintariani Firsta Sesarina Mintariani Firsta Sesarina Mintariani Fitri NPE G. M. D. Putra I Gde Pande Aninditha Putra Wicaksana I Gusti Made Aman I Gusti Ngurah Agung Dewantara Putra I Gusti Ngurah Jemmy Anton Prasetia I Made Agus Gelgel Wirasuta I Nyoman Hariyasa Sanjaya I Nyoman Hariyasa Sanjaya I Wayan Martadi Santika I Wayan Putu Sutirta Yasa I. N. K. Widjaja Ida Bagus Made Oka L.P.M.K. Dewi Lestari, Ni Wayan Febriari Liyani, Kadek Yunita Luh Putu Febryana L Luh Putu Febryana Larasanty Luh Putu Febryana Larasanty M. D. Abimanyu M. R Sutharini Made Diah Vendita Sakuntari Made Diah Vendita Sakuntari Made Diah Vendita Sakuntari Made Krisna Adi Jaya N. K. Cahyaningsih N. K. M. Ariani, N. L. P. V. Paramita N. P. A. D. Wijayanti N. P. A. D. Wijayanti N. W. M Lina N.M.P. Susanti Ni Komang Anik Pirgantari Ni Komang Anik Pirgantari Ni Komang Anik Pirgantari Ni Komang Cahyaningsih Ni Luh Gede Lisniawati Ni Luh Made Diah Mas Cahyani Putri Ni Luh Made Diah Mas Cahyani Putri Ni Luh Made Diah Mas Cahyani Putri Ni Luh Md Dwi Laxmi Satriani Ni Luh Md Dwi Laxmi Satriani Ni Luh Md Dwi Laxmi Satriani Ni Nyoman Fabby Sukarmini Ni Nyoman Mahatriny, Ni Nyoman Ni Nyoman Wiwik S Ni Nyoman Wiwik S. Ni Putu Ariantari Ni Putu Ayu Dewi Wijayanti Ni Putu Linda Laksmiani Ni Putu Nining Gianni Ni Putu Nining Gianni Ni Putu Nining Gianni Ni Putu Sanggra Payani, Ni Putu Ni Wayan Dewi Purwanti Ni Wayan Dewi Purwanti Ni Wayan Dewi Purwanti Nirmalayanthi, Putu Ayuning Dinda Pande Ketut Suwanti Devi, Pande Ketut Putu Ita Purnama Dewi R. A. I. K. Maharani Rasmaya Niruri Rini Noviyani Sagun Chandra Yowani Santri Yulita Santri Yulita, Santri Siwananda, I Dewa Made T. Maharani Warditianin, Ni Kadek Widhyasih, Retno Martini Wiwik S, Ni Nyoman Yeyet C. Sumirtapura Yeyet C. Sumirtapura Yeyet C. Sumirtapura

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