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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biopropal Industri BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi Indonesia Makara Journal of Science Makara Journal of Technology Jurnal Mahasiswa Mengabdi (JIMAWAbdi) JUPADAI : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Ibnu Sina Jurnal Manajerial dan Bisnis Tanjungpinang Sharia Economic and Management Business Journal (SEMBJ) Journal Of Chemical Process Engineering Annales Bogorienses
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Lembaga Ilmu Pengetahuan Indonesia
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Journal : Makara Journal of Technology

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Substrates Preparation from Woody Tropical Waste Biomass for Biohydrogen Production Susilaningsih, Dwi; Harwati, Theresia Umi; Anam, Khairul; Yopi, Yopi
Makara Journal of Technology Vol. 12, No. 1
Publisher : UI Scholars Hub

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Substrates Preparation from Woody Tropical Waste Biomass for Biohydrogen Production. Addressing to the global warming problem, energy crisis and pollution, hydrogen production by micro-organisms using biotechnological approach should be considered, since it fulfils the recent society requirement to safely produce, renewable and environmental friendly energy. Hydrogen is one of the most promising green energy sources, because it is easily converted to electricity and cleanly combustible. There are three types of micro-organisms for hydrogen production, the first is cyanobacteria through the photosynthesis process, the second is anaerobic bacteria, which use organic substances as electron donor and energy and convert them to hydrogen, the third is photosynthetic bacteria, somewhat between photosynthetic and anaerobic bacteria, which are capable of converting the organic substances to hydrogen at a fairly high rate. We propose to use the abundant waste biomasses in Indonesia for hydrogen production by the microbial system. Our focus research is the production of hydrogen from waste biomasses by two-stage fermentation systems, which combine the conversion process of monomer biomasses to lactic acid by Lactobacillus sp. and the conversion process of lactic acid to hydrogen by photosynthetic bacteria. In this research, two kind substrates preparation were apply for woody waste biomass such as chemical hydrolysis and biological methods with several treatments. The results of the substrate preparation state showed that hydrolyses process of biomasses using strong acid are yielded total sugar about 70-90% of previous original content. Moreover, hydrolyses process using weak/diluted acid are yielded total sugar about 4-30% of original sugar. Furthermore, the biological treatments of degradation of woody waste biomasses are yielded total sugar about 0-10% (by single culture) and 10-50% (by consortium). Those hydrolysates substrates will use for fermentation two stages of lactate fermentation and conversion by photosynthetic bacteria in order to produce hydrogen gas.
Sugarcane Bagasse as a Carrier for the Immobilization of Saccharomyces cerevisiaein Bioethanol Production Anita, Sita Heris; Mangunwardoyo, Wibowo; Yopi, Yopi
Makara Journal of Technology Vol. 20, No. 2
Publisher : UI Scholars Hub

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Sugarcane bagasse was used as a carrier to immobilize Saccharomyces cerevisiae in bioethanol production. This research aims to study the potential use of sugarcane bagasse as an alternative carrier for cell immobilization and improvement in the production process of cell immobilization in bagasse. The results showed that the physical characteristics of sugarcane bagasse as a carrier were water content (7.77 ± 0.35%), water retention (4.80 ± 0.44 g/g), water absorption index (8.58 ± 0.22 g/g), and lignin content (24.40 ± 1.52 %). Determination of cell retention was performed in an inoculum volume of 50 mL yeast suspension with various carrier weights (2.5, 5, 10, and 20 g). The highest cell retention was obtained in ratio of 2.5 g carrier/50 mL cell suspension with cell retention of 5.41 ± 1.06 mg/g, or known as biocatalyst. Biocatalyst, as much as 1.5, 3, 4.5, and 6 g, were used as inoculum for a 24 hour bioethanol fermentation. The best concentration and productivity of bioethanol, obtained by using 3 g of biocatalyst, were 23.95 ± 0.28 g/L and 1.24 ± 0.01 g/L/hours. The average of bioethanol yield for a 24 hour fermentation by using immobilized cells was three times higher than the free cells system.
Co-Authors A.A. Ketut Agung Cahyawan W ADE ANDRIANI Agung Pranata, Gandi Ahmad Thontowi Anam, M. Khairul Anja Meryandini Awan Purnawan Bambang Prasetya Budiawan Budiawan Dewi, Fitria DWI SUSILANINGSIH Elvi Yetti, Elvi Endang Sukara Gulo, Happy Christman Hapzi Ali Harayama, Shigeaki Harayama, Shigeaki Harwati, Theresia Umi Helprida Simamora Hidayat, Haris Suganda Ilmiah, Zidny Jonmaianto Sihombing La Ifa Maggy T Suhartono Maggy Thenawidjaja Suhartono Martin, Andri Fadillah Muhammad Iqbal NANIK RAHMANI Nuryana, Isa Nuryati Nuryati Nuzul Farini, Nuzul Okazaki, Fumiyoshi Okazaki, Fumiyoshi Oktavianti Oktavianti Palupi, Nurheni Sri Purwoko, Agus PUSPITA LISDIYANTI Putra, Filemon Jalu Nusantara Putri, Feby Heryani Rahmasari, Dianti Rahmasari, Dianti Riznando, Reza Rohmattusolihat Rohmattusolihat, Rohmattusolihat ROHMATUSSOLIHAT ROHMATUSSOLIHAT Ruth Melliawati Safitriani, Safitriani Safitriani, Safitriani Sari, Yana Nurita Sari, Yana Nurita Septa Diana Nabella SHANTI RATNAKOMALA Sihombing, Jonmaianto Sita Heris Anita Sri Hartati Sri Pujiyanto Suhanda La Tima, Suhanda Sulastri Sulastri Suryani Suryani Susiana Purwantisari Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Theresia Harwati, Theresia Tun Tedja Irawadi Tutik Murniasih Urip Perwitasari, Urip Wahyudi, Muhammad Eko WIBOWO MANGUNWARDOYO Wijanarka Wijanarka Wijaya, Hans Yantyati Widyastuti