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EFFECT OF POST-HATCH FEED DEPRIVATION ON FATTY ACID COMPOSITION OF BROILER MEAT Sugiharto, S.; Isroli, I.; Yudiarti, T.; Widiastuti, E.; Kusumanti, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 4 (2015): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.4.234-237

Abstract

The objective of the present study was to investigate the effect of feed deprivation over the first 48 h post-hatch on the composition of fatty acids in broiler meat. Sixty 1-d-old Ross chicks were divided to two experimental groups, i.e., birds provided access to feed and water ad libitumimmediately post-hatch until d 35 and birds deprived from feed but not from water over the first 48 h post-hatch. Blood for cholesterol analysis was collected on d 34. The same birds were sacrificed on d 36, and immediately breast meat, abdominal fat, heart and liver were collected and weighed.Feed deprivation over the first 48 h post-hatch retarded (P<0.05) growth performance of chickens, but had no impact (P>0.05) on the abdominal fat, fatty acid profile of meat, total serum cholesterol level as well as the weight of heart and liver of broiler. In conclusion, early post-hatch feeding is a beneficial means to enhance the final body weight of broilers without affecting the meat quality.
The potential of Bacillus strains isolated from the rumen content of dairy cows as natural antibacterial and antioxidant agents for broilers Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H.I.; Suprijatna, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.115-123

Abstract

The study was conducted to investigate the in vitro antibacterial activity and antioxidant-enhancing effect of Bacillus strains isolated from the rumen content of dairy cows. The antibacterial activity of Bacillus strains was assessed against Escherichia colibased on the agar-well diffusion method. To assess the antioxidant-enhancing effect, the Bacillusstrains was mixed (prepared as multi-strains) and used to ferment herbal medicine waste (HMW). The effect was compared with that of commercial probiotic Bacillus subtilis and non fermented HWM. Results showed that each Bacillus strain was able to inhibit the growth of E. coli. Fermentation with mixed Bacillus strains tended (P=0.07) to enhance the 2,2-diphenylpicrylhydrazyl (DPPH) radicals scavenging activity and total phenolics content of HMW. In contrast, fermentation with commercial Bacillus subtilis tended (P=0.07) to decrease DPPH radical scavenging activity and total phenol of HMW. Fermentation with both Bacillus preparations increased (P<0.05) the contents of crude protein and ash in HMW, while crude fat increased  (P<0.05) in HMW fermented with Bacillus subtilis. Fermentation either with Bacillus subtilis or mixed Bacillus strains increased (P<0.05) the fiber content of HMW. In conclusion, Bacillus strains isolated from the rumen content of dairy cows showed antibacterial activity and antioxidant-enhancing effect, therefore, they are potential as feed additive to substitute synthetic antibiotics and antioxidants for broilers raised under tropical conditions.
IDENTIFICATION OF SOIL FUNGI ISOLATED FROM ALFALFA (Medicago sativa L) TO FIND SPECIFIC FUNGI WHICH IMPROVED THE GROWTH OF ALFALFA Yudiarti, T.; Sumarsono, S.; Widjayanto, D.W.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 3 (2010): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.3.197-200

Abstract

Objective of the study was to identify all kinds of fungi which can life in the alfalfa plantation inBaturaden Purwokerto-Central Java. Fungi used in this study was 38 isolates. All fungi have been takenfrom the isolation of soil and root of diseased plant. Macroscopic and microscopic methods were usedfor identification. Potato Dextrose Agar (PDA) medium was used to grow the fungi. All fungi wereidentified using book identification of fungi. The results showed that from 38 isolates, six species wasdetermined and one was unidentifed. Those species identified were Cuninghammela sp, Trichoderma sp,Vertilicium sp, Eupenicillium sp, Pythium sp, Aspergillus sp.
Body weight, haematological indices and intestinal morphometric of broilers provided with diets containing formic acid, butyric acid or their blends Isroli, I.; Murwani, R.; Yudiarti, T.; Widiastuti, E.; Wahyuni, H. I.; Sartono, T. A.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 1 (2020): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.1.37-46

Abstract

The study investigated the influence of dietary incorporation of formic acid, butyric acid or their blends on growth rate, haematological indices and intestinal morphometric of broilers. A number of 240 Lohmann MB-202 chicks were pass rounded to four dietary groups, including CONT (chicks taking in basal feed with no additive), BTRT (chicks receiving basal feed with 0.03% butyric acid), FRMT (chicks receiving basal feed with 0.1% formic acid) and BTRT+FRMT (chicks receiving basal feed containing 0.03% butyric acid and 0.1% formic acid). Weight of chicks and intake were measured every week, while blood sample was collected at day 21 and 35. At day 35, birds were slaughtered and small intestinal segments and digesta were collected. At day 21, body weight was bigger (P<0.05) in BTRT+FRMT than in other birds. Feeding the blends of formic and butyric acids resulted in higher (P<0.05) value of mean corpuscular haemoglobin concentration (MCHC) at day 21. Feeding organic acids was associated with the lower (P<0.05) proportion of thrombocytes in broilers at day 35. Supplementation of butyric acid resulted in higher (P<0.05) serum albumin concentration at day 35. Butyric acid produced higher (P<0.05) levels of triglyceride as well as low-density lipoprotein (LDL) in the serum. The mixture of formic and butyric acids resulted in higher (P<0.05) titer of antibody against Newcastle disease vaccine (NDV) at day 21. Feeding formic acid decreased (P<0.05) ileal population of lactose negative enterobacteria at day 35. Overall, dietary supplementation of the blends of formic and butyric acids resulted in improved growth and immune defence of broiler chicks. 
ISOLATION OF FUNGI FROM THE GASTROINTESTINAL TRACT OF INDIGENOUS CHICKEN Yudiarti, T.; Yunianto B.I., V.D.; Murwani, R.; Kusdiyantini, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.115-120

Abstract

Gastrointestinal tract of chicken is a place in which many kinds of fungi can be found. The aim ofthe research was to isolate fungi from the gastrointestinal tract of the indigenous chicken (AyamKampung). The chicken samples were four days, one week and two months old and were sampled fromchicken farm located in Yogyakarta. Potato dextrose agar (PDA) medium was used to grow the fungi.Fifty pure isolates of fungi were found from three different ages, those were four days, one week andtwo months old chicken were 5, 10 and 35 isolates respectively. The largest number of isolate was foundin ileum, then followed by caecum, jejenum and duodenum. The fifty isolate of fungi belonged to sevenspecies, those were Aspergillus fumigatus, Aspergillus niger, Chrysonilia crassa, Mucor circinelloides,Mucor sp, Rhizopus oligosporus and Rhizopus oryzae.
Intestinal morphology and growth performance of the Indonesian indigenous crossbred chickens supplemented with formic acid and Saccharomyces cerevisiae Erya, S. N.; Wahyuni, H. I.; Yudiarti, T.; Widiastuti, E.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 4 (2020): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.4.348-355

Abstract

The study investigated gut ecology and morphology of the Indonesian indigenous crossbred chickens (IICC) supplemented with the combination of formic acid and Saccharomyces cerevisiae. Two hundreds day-old IICC were distributed to T0 (control diet), T1 (T0 + 0.2% formic acid), T2 (T0 + 0.3% S. cerevisiae), T3 (T0 + 0.2% formic acid and 0.3% S. cerevisiae). Excreta was collected at week 8, while intestinal ecology and morphology were determined at week 9. In duodenum, T3 chicks showed higher and wider (P<0.05) villi. The T2 and T3 chicks showed deeper (P<0.05) crypt than that of T0. The jejunal villi was higher (P<0.05) in T3 than in T0. The T3 chicks had deeper (P<0.05) crypt compared to other groups. In ileum, the villi height was lowest (P<0.05) in T0. The crypt was deeper (P<0.05) in T3 than in other. Crude protein digestibility coefficient was highest (P<0.05), while fecal protein was loswest (P<0.05) in T1 than in other groups. Compared to T0, the treated IICC showed higher (P<0.05) weight gain and feed intake with T3 had the highest gain and intake but gain:feed rasio was the lowest (P>0.05). IIn conclusion, the inclusion of formic acid and S. cerevisiae in diets improved intestinal ecology and morphology. The IICC chickens fed with formic acid and S. cerevisiae exhibited improved growth performance and nutrient digestibility. 
Improving the Nutritional Values of Cassava Pulp Through Supplementation of Selected Leaves Meal and Fermentation with Chrysonilia Crassa Sugiharto, Sugiharto; Widiastuti, Endang; Yudiarti, Turrini; Wahyuni, Hanny Indrat; Sartono, Tri Agus
ANIMAL PRODUCTION Vol. 23 No. 2 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.2.49

Abstract

This study evaluated the impact of fermentation with Chrysonilia crassa on nutritional composition and antioxidative activity of the mixture of cassava pulp and selected leaves meal. Cassava pulp (60 g) was mixed thoroughly with 35 g leaves meal of either cassava, M. Oleifera, or L. leucocephala, and inoculated with Chrysonilia crassa starter (5 g). The mixture was aerobically incubated for 3 days at room temperature and analyzed for the proximate composition, gross energy, true protein, antioxidant activity, and amino acid content. The fermented products showed higher crude protein, ether extract, ash, and gross energy but lower fibre content than unfermented cassava pulp. Both leaves meal supplementation and fermentation increased the true protein content of cassava pulp. Superior antioxidant activities and higher amino acids were observed in the fermented products than raw cassava pulp. In conclusion, supplementation of leaves meal in conjunction with Chrysonilia crassa-fermentation improved the nutritional values and antioxidant potential of cassava pulp.
Effect of sprouted papaya seed meal on physiological conditions, intestinal bacterial populations and meat quality of broilers Sugiharto, S.; Winarti, W.; Widiastuti, E.; Yudiarti, T.; Sartono, T. A.; Wahyuni, H. I.; Pratama, A. R.
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 1 (2022): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.1.35-43

Abstract

The study investigated the effect of sprouted papaya seed meal (SPSM) on physiological conditions, intestinal bacteria and meat quality of broilers. A 390 broiler chicks were distributed to T0 (control feed), T1 (feed with 2.5% papaya seed meal [PSM]), T2 (1% SPSM), T3 (2.5% SPSM), and T4 (5% SPSM). Blood, intestinal digesta and meat were obtained at day 36. Feeding 2.5% PSM lowered (P<0.05), but SPSM up to 5% had no effect on daily gain. PSM reduced (p<0.05) feed intake, but not SPSM. Feed efficiency was lower (P<0.05) in T4. Feeding 5% SPSM increased (P=0.06) bursa of fabricius. T1, T3 and T4 had lower (P=0.09) heterophils. Mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were lower (P<0.05) in T4. Cholesterol to high-density lipoprotein (HDL) ratio of PSM and SPSM was lower (P<0.05) than control. SPSM at 2.5% increased (P<0.05) serum HDL. PSM-fed birds had lower cholesterol (P=0.07), triglyceride (P=0.09) and lowdensity lipoprotein (P=0.09). PSM or SPSM decreased (P<0.05) serum total protein, albumin and globulin. PSM and SPSM reduced (P<0.05) creatinine. Alanine aminotransferase was reduced (P<0.05) with SPSM at 1 and 2.5%. Ileal lactic acid bacteria to coliform ratio in PSM and SPSM was greater (P<0.05) than in control. Ileal coliform was lower (P=0.08) in PSM and SPSM. PSM reduced (P=0.08) saturated fatty acids, while 1 and 2.5% SPSM increased (P=0.09) unsaturated fatty acids contents of meats. In conclusion, SPSM improved immune competence, blood lipid profile and gut bacterial population of broilers.
Effect of Averrhoa bilimbi Fruit Filtrate and Shrimp Paste Mixture on Performance, Gut Microbes and Blood Profile of Broilers Indri Mareta; Gilbert Nathaniel; Turrini Yudiarti; Endang Widiastuti; Hanny Indrat Wahyuni; Sugiharto Sugiharto
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 4 (2020): December 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i4.2515

Abstract

This study was aimed to evaluate effect of a mixture of Averrhoa bilimbi fruit filtrate and shrimp paste (Mysis sp.) on the growth performance, blood profile, selected intestinal bacterial number and pH value of broiler digestive tract. The mixture of A. bilimbi fruit filtrate and shrimp paste were incubated for 4 days and were then used in the experiment. For in vivo experiment, 40 day-old Lohmann broiler chicks were distributed randomly to two treatment groups, i.e., control (T1) and chickens given the mixture of 10% A. bilimbi fruit filtrate and shrimp paste in drinking water (T2). Body weight and feed intake were recorded weekly. At day 42, 2 birds from each pen (8 chicks per treatment group) were taken for blood and digesta collection. Internal organ weight and carcass traits were determined thereafter. Feed conversion ratio (FCR) was lower (P<0.05) on treatment group than control. Hemoglobin and mean corpuscular volume (MCV) of treatment group were higher (P<0.05) than that of control. Total cholesterol was higher (P<0.05) in treatment group than that in control. Total cecum coliform was lower (P<0.05) in treatment group than that in control. The pH values of the small intestinal segments (jejunum, ileum, cecum) was lower (P<0.05) in treatment group than that in control group. In conclusion, administration of the blends of A. bilimbi fruit filtrate and shrimp paste into drinking water improved FCR, increased hemoglobin and MCV values, decreased gut pH and cecal coliform of broiler chickens.
Jumlah Coliform, BAL dan Total Bakteri Usus Halus Ayam Broiler yang Diberi Kunyit (Curcuma domestica) R. Halimatunnisroh; T. Yudiarti; Sugiharto Sugiharto
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 19, No 2 (2017): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.19.2.79-84.2017

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian air rebusan kunyit terhadap total bakteri coliform, bakteri asam laktat (BAL) dan total bakteri dalam usus halus ayam broiler. Materi yang digunakan yaitu 200 ekor ayam broiler DOC (Day Old Chick), strain Lohman, bobot badan rata-rata 41,48 ± 0,99 g. Ternak dipelihara selama 35 hari dan perlakuan pemberian air rebusan kunyit pada air minum mulai diberikan pada umur 11 hari. Rancangan penelitian yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan, setiap ulangan terdiri dari 8 ekor. Perlakuan yang digunakan yaitu T0 (100% air), T1 (25% air rebusan kunyit : 75% air), T2 (50% air rebusan kunyit : 50% air), T3 (75% air rebusan kunyit : 25% air), dan T4 (100% air rebusan kunyit). Parameter yang diamati yaitu jumlah coliform, BAL, dan total bakteri dalam usus halus. Hasil penelitian menunjukkan bahwa pemberian air rebusan kunyit pada air minum ayam broiler berpengaruh nyata (p<0,05) terhadap jumlah BAL dan total bakteri, tetapi tidak berpengaruh (p>0,05) terhadap jumlah coliform. Keimpulannya, bahwa pemberian air rebusan kunyit tidak meningkatkan/menurunkan total bakteri coliform tetapi menurunkan total bakteri dan meningkatkan total BAL.