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Yurliasni Yurliasni
Syiah Kuala University
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Journal : Jurnal Agripet

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Potensi Khamir Kluyveromyces lactis dalam Meningkatkan Kualitas Susu Fermentasi yang Ditambah Jagung Manis (Zea mays saccharata Linn) Siti Rani Ayuti; Yurliasni Yurliasni; Cut Intan Novita; Elmy Mariana
Jurnal Agripet Vol 20, No 1 (2020): Volume 20, No. 1, April 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i1.15356

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui peningkatan kualitas produk susu fermentasi dengan menggunakan khamir Kluyveromyces lactis dan penambahan jagung manis. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor dan 2 level, yaitu faktor penambahan jagung manis (Zea mays saccharata Linn) (A) terdiri atas 25% (a1) dan 75% (a2), dan faktor penambahan khamir Kluyveromyces lactis (B) terdiri atas 0.5% (b1) dan 1% (b2), dengan lima kali ulangan pada masing-masing perlakuan sehingga memperoleh 20 satuan percobaan. Parameter yang diamati antara lain adalah kadar protein, kadar lemak, kadar asam laktat dan derajat keasaman (pH). Data yang diperoleh dianalisis secara statistik menggunakan ANOVA dan dilanjutkan dengan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa penambahan khamir Kluyveromyces lactis tidak berpengaruh nyata (P0.05) terhadap kadar protein, lemak, kadar asam laktat dan derajat keasaman (pH). Sementara penambahan jagung manis tidak berpengaruh nyata (P0.05) terhadap kadar protein dan derajat keasaman (pH), namun berpengaruh sangat nyata (P0.01) terhadap kadar lemak dan kadar asam laktat. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan khamir Kluyveromyces lactis 1% dan jagung manis (Zea mays saccharata Linn) 75% pada produk susu fermentasi dapat meningkatkan kualitas produk susu.(Potential of yeast-Kluyveromyces lactis in improving quality of fermented milk added sweet corn (Zea mays saccharata Linn))ABSTRACT. The aims of this research were to determine the quality improvement of fermented milk products using Kluyveromyces lactis and the addition of sweet corn. This study used factorial experiment conducted with completely randomized design (CRD) which divide into 2 factors and 2 levels, there were the addition of sweet corn (Zea mays saccharata Linn) factors (A) consist of 25% (a1) and 75% (a2), and the addition of yeast Kluyveromyces lactis factors (B) consist of 0.5% (b1) and 1% (b2), with five replications on each treatment to obtain 20 units of experiment. The observed parameters such as protein levels, fat levels, lactic acid levels and acidity degree (pH). Research data were analyzed statistically by using ANOVA and continued by Duncan's tests. The results showed that the addition of yeast Kluyveromyces lactis had no significant effect (P 0.05) on protein levels, fat levels, lactic acid levels and acidity degree (pH). While the addition of sweet corn had no significant effect (P 0.05) on protein levels and acidity degree (pH), but it had a very significant effects (P 0.01) on fat levels and lactic acid levels. The results indicate the addition of yeast Kluyveromyces lactis 1% and sweet corn (Zea mays saccharata Linn) 75% in fermented milk products can improve the quality of dairy products.
Pengaruh Perbedaan Suhu Pemanasan terhadap Aktivitas Antibakteri Susu Kambing Fermentasi Menggunakan Lactobacillus Plantarum Zuraida Hanum; Yurliasni Yurliasni; Sitti Wajizah; Juanda Juanda
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14330

Abstract

ABSTRAK. Penelitian ini bertujuan untuk melihat pengaruh suhu pemanasan yang berbeda terhadap aktivitas antibakteri susu kambing fermentasi menggunakan starter Lactobacillus plantarum. Bakteri uji yang digunakan berasal dari golongan Enterobacteriaceae yaitu Escherichia coli dan Salmonella.sp. Rancangan penelitian yang digunakan adalah RAL (Rancangan Acak Lengkap) Faktorial yang terdiri dari 2 faktor yaitu faktor A adalah suhu pemanasan, yaitu a1 (suhu pemanasan 72C), a2 (suhu pemanasan 121C) dan faktor B adalah level penambahan Lactobacillus plantarum yaitu perlakuan b1 (Lactobacillus plantarum 2,5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7,5%), masing-masing perlakuan diulang sebanyak 3 kali sehingga diperoleh 18 unit percobaan. Apabila terdapat perbedaan yang nyata maka dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan adanya interaksi dari suhu pemanasan yang berbeda dan penambahan L. plantarum pada susu kambing berpengaruh nyata terhadap kadar asam laktat dan daya hambat pada Salmonella sp, akan tetapi tidak berpengaruh nyata terhadap E. coli. Kesimpulan dari penelitian ini diperoleh suhu pasteurisasi dengan penambahan 7.5 % L.plantarum merupakan nilai terbaik dalam menghambat Salmonella dan E..coli.(Effect of different heating temperature on antibacterial activity of goat milk fermented with Lactobacillus Plantarum)ABSTRACT.This study aimed to determine the effect of the different heating temperatures on inhibitory activity of fermented goat's milk with Lactobacillus plantarum. The test bacteria used were Enterobacteriaceae, namely Escherichia coli and Salmonella.sp.The design applied is CFD (Completely Randomized Design) consisting of 2 factors, namely factor A heating temperature with two levels a1 (72C), a2 (121C) and factor B is the level of Lactobacillus plantarum addition by three levels, namely b1 (Lactobacillus plantarum 2.5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7.5%), and the treatments were repeated 3 times. The results showed that different heating temperature and level of L. plantarum added in fermented goat milk significantly affected the levels of lactic acid and inhibitory power on Salmonella sp, but not on E. coli. In conclusion is different heating temperatures and addition of L. plantarum on goat milk at significantly affected on inhibitory power on Salmonella sp, but not on E. coli. The results obtained pasteurisasi with 7.5% L.plantarum could be applied.
Dinamika Pertumbuhan Lactobacillus casei dan Karakteristik Susu Fermentasi Berdasarkan Suhu dan Lama Penyimpanan Siti Rani Ayuti; Nurliana Nurliana; Yurliasni Yurliasni; Sugito Sugito; Darmawi Darmawi
Jurnal Agripet Vol 16, No 1 (2016): Volume 16, N0. 1, April 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v16i1.3476

Abstract

ABSTRAK. Mutu susu fermentasi sangat dipengaruhi oleh bahan baku, proses pengolahan, proses fermentasi, dan penyimpanan. Perubahan nilai gizi dapat terjadi karena variasi dan fluktuasi suhu dan penyimpanan akan mempercepat kerusakan susu fermentasi. Penelitian bertujuan untuk mempelajari pengaruh suhu dan lama penyimpanan yang berbeda pada susu fermentasi terhadap pertumbuhan L. casei dan karakteristik susu fermentasi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor yaitu faktor A (suhu) terdiri dari a1=4-10C, a2=10-16C. Faktor B (lama penyimpanan) terdiri b1=0 hari b2=30 hari, b3=60 hari, b4=90 hari dan dianalisis meliputi jumlah total bakteri L. casei, nilai derajat keasaman (pH), kadar asam laktat, sineresis, dan kadar alkohol. Hasil analisis menunjukkan bahwa perlakuan suhu dan lama penyimpanan berpengaruh sangat nyata (P0.01) terhadap total bakteri, sineresis, kadar alkohol, asam laktat, tetapi pada pH terjadi interaksi antara suhu dan penyimpanan. Disimpulkan bahwa suhu dan lama penyimpanan dapat mengakibatkan pertumbuhan L. casei tidak stabil dan terjadinya perubahan karakteristik fisik susu fermentasi L. casei.(Growth dynamics of Lactobacillus casei and characteristics of milk fermentation based on temperature and storage time)ABSTRACT. Fermented milk quality is strongly influenced by raw materials, processing, fermentation and storage. Changes in nutritional value may occur due to variations and fluctuations in temperature and storage will accelerate damage fermented milk. The research aimed to study the effect of temperature and different storage length of fermented milk on the growth of L. casei and characteristics of fermented milk. The study used completely randomized design (CRD) with factorial pattern which are consisted of two factors. Factor A (storage temperature) they were a1=4-10C, a2=10-16C. Factor B (storage time) they were b1=0 days, b2=30 days, b3=60 days, b4=90 days and analyzed include the total number of bacteria L. casei, the value of the degree of acidity (pH), lactic acid levels, syneresis, and alcohol content. The results showed that the temperature and storage time was highly significant (P0.01) effect the total bacteria, syneresis, alcohol level, lactic acid level, and there was interaction between temperature and storage time on pH. It can conclude that the temperature and storage time affect the growth of L. casei unstable and changes in the physical characteristics of L. casei fermented milk.
Pengaruh Warna Kerabang Dan Lama Penyimpanan Pada Suhu Beebeda Terhadap Kualitas Suhu Konsumsi Yurliasni Yurliasni; Herawati Latif
Jurnal Agripet Vol 7, No 1 (2007): Volume 7, No. 1, April 2007
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v7i1.3310

Abstract

ABSTRACT.The objective of this research is to learn the effect of shell color and storage length on egg quality. The research was held at the Animal Nutrion Laboratory Departement of Agriculture, Syiah Kuala University. The treatments were shell color 30 SC (dark brown): 30-40 SC (white) with 10 replication and store in 270C and 100C for 35 days. The observation was taken every 7 days. Parameters measured were foam ability and Haugh Unit by group random sampling design with factorial pattern. The result shows that temperature and storage length significantly effect foam ability but not in haugh unit. Storage temperature and storage temperature at 100C show that foam ability is better with the average of 422.31 cm3. Compared to 270C with the average of 394.04 cm3. On the other hand shell color has no effect both on foam ability and Haugh Unit. In general, shell color, storage length and storage temperature have no effect on egg quality.
Factors Influencing Imunity and Resistance of Pediococcus Acidilactici to Bacteriocin, Pediocin AcH Yurliasni Yurliasni
Jurnal Agripet Vol 2, No 1 (2001): Volume 2, No. 1, April 2001
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v2i1.3088

Abstract

ABSTRACT Di dalam pediaosin Ach yang dihasilkan oleh bakteri Pediococcus acidilactici, kedua gen untuk produksi pediosin dan kekebalan terhadap pediosin dikode oleh plasmid pSMB74 pada 8,9 kb. Dengan hilangnya plasmid ini dapat menghilangkan kemampuan bakteri untuk mengahsilkan pediosin AcH, tetapi beberapa masih bertahan terhadap pediosin. Bakteri yang bertahan adalah jenis antara yaitu diperoleh pada saat sel yang tidak dapat berproduksi ditumbuhkan pada media yang mengandung pediosin AcH, tetapi hilang ketika sel ditumbuhkan tanpa adanya pediosin AcH.
Nilai Nutrisi Dadih yang ditambahkan Khamir Asal Dadih Yurliasni Yurliasni; Yusdar Zakaria; Yunasri Usman
Jurnal Agripet Vol 14, No 2 (2014): Volume 14, No. 2, Oktober 2014
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v14i2.1891

Abstract

(Nutritive value of dadih added with yeast of dadih origin)ABSTRACT. Dadih is traditionally fermented buffalo milk product which is along with lactic acid bacteria (LAB), a diversity of yeast species involved in fermentation process. The existence of yeast in dadih gives positive contribution either in aroma or their role in securing a good product. There were three different dadih origin yeasts involved in this experiment (Candida curiosa, Brettanomyces custersii and Kluyveromyces lactis.) The objective of this research is to study more about growth and yeasts role on dadih fermentation in order to find positive value of yeast to keep nutritive value of dadih. Completely Randomized Designed used with four treatments. The treatments given was percentage of combined yeast culture which were P0 (buffalo milk + LAB), P1 (buffalo milk + LAB + 0,5% yeast culture), P2 (buffalo milk + LAB + 1,0% yeast culture), and P3 (buffalo milk + LAB + 1,5% yeast culture), by seven replication. The difference effect between treatment was tested by using Duncans Multiple Range Test. The Results showed that addition of yeast culture in dadih effect the pH, total of microorganism, degree of lactic acid, protein and dadih fat significantly. Overall dadih added with 1% yeast culture (P2) increase 14% degree of protein and decrease 2,7% degree of dadih fat.
Analisa Keasaman dan Total Bakteri Asam Laktat Yogurt Akibat Bahan Baku dan Persentase Lactobacillus casei yang Berbeda Yusdar Zakaria; Yurliasni Yurliasni; Mira Delima; Ely Diana
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i2.817

Abstract

Analyze of acidity and amount of lactic acid bacteria of yogurt made from different milk types and lactobacillus casei percentageABSTRACT. The objective of this experiment is to determine the effect of milk types and percentages of Lactobacillus casei as a starter to the amount of Lactic Acid Bacteria in yogurt and its pH value and Lactic Acid value. Factorial Completely Randomized Design with two factors and three replications was applied. The A factor was the milk types that were a1 = Powder Milk, a2 = Fresh Milk and a3 = UHT milk. The B factor was the percentages of Lactobacillus casei (b1 = 5% and b2 = 10%). Parameters observed were the level of lactic acid, the pH value of yogurt and the amount of lactic acid bacteria. The result showed significantly difference (P0.01) from the use of milk types and Lactobacillus casei percentages on the lactic acid level, and there are no interaction between factors. There was also significant different (P0.01) showed from pH value and an interaction between both factors was presented. In addition there was significant difference (P0.05) showed by the amount of lactic acid bacteria, and an interaction between both factors was also existed. The research concluded that the higher the percentage of starter, the higher the level of lactic acid and the amount of lactic acid bacteria of yogurt that made from all milk types. On the contrary, the higher the percentage of starter, the lower the pH value of yogurt.
Aktivitas Antimikroba Khamir Asal Dadih (susu kerbau fermentasi) Terhadap Beberapa Bakteri Patogen Yurliasni Yurliasni
Jurnal Agripet Vol 10, No 1 (2010): Volume 10, No. 1, April 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v10i1.633

Abstract

Antimicroba activity of yeast dadih (buffalo milk fermentation) origin on pathogenic bacteriaABSTRACT. Research was carried out to study the effect of antimicrobial activity of yeast found in dadih. Yeasts chosen were isolated from dadih (traditionally buffalo milk fermentation). The objective is to know the ability of yeast to inhibit the growth of pathogenic bacteria in milk. They were Candida curiosa, Brettanomyces custersii, and Kluyveromices lactis. Kluy. lactis has strong antimicrobial on Bacillus subtilis with zone of inhibition 5mm, C. curiosa on E.coli (5mm) and C. curiosa and Brett. custersii also have strong antimicrobial on S. aureus consecutively 5,75 and 7 mm. As a conclusion the result showed that all of three yeasts selected have capability to stop the growth of pathogenic bacteria of buffalo milk origin in five days.
Co-Authors . Darmawi . Nurliana Aini, Zahratul Cut Aida Fitri Cut Intan Novita Cut Intan Novita Cut Intan Novita Dea Lestari Dzarnisa Araby Dzarnisa Dzarnisa Elmy Mariana Elmy Mariana Ely Diana Fajri Fajri Feby Zalika Parindra Hasanul Bahri Herawati Latif Herawati Latif Jinnani, Rida Raihatil Juanda Juanda Khalida, Qoryna Linda Yanti Hutasuhut Lukman Hakim Marpaung, Deva Aryana Mira Delima Mira Delima Mohd. Agus Nashri Abdullah Muhammad Daud Muhammad Yasar Nendi Junaedi Nurul Ayu Agustina Nurul Hidayati Ridha Hikmawan Rifni, Ulvira Romano Romano Samadi Samadi Sarlini Martha Sitepu, Sukma Aditya Siti Anggaini Siti Rani Ayuti Sitti Wajizah Sitti Wajizah Sitti Wajizah Sugito Sugito Suyanti Kasimin Tri Handayani Vaudhan Fuady Widyawati Widyawati Windari, Trisna Yunasri Usman Yusdar Zakaria Yusdar Zakaria Zahratul Aini Zakiah Zakiah Zakki Muhtaram Zuraida Hanum Zuraida Hanum Zuraida Hanum Zuraida Hanum Zuraida Hanum