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THE EFFECT OF DIFFERENT DOSES OF Bacillus sp. PROBIOTICS ON THE SURVIVAL RATE OF WHITE SNAPPER (Lates calcarifer) LARVAE Dewita Sari, Ida Ayu Sawitri; Swasta, Ida Bagus Jelantik; Amelia, Jasmine Masyitha
Aquasains: Jurnal Ilmu Perikanan dan Sumberdaya Perairan Vol. 14 No. 1 (2025): November 2025
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aqs.v13i1.10743

Abstract

This study aims to analyze the effect doses of Bacillus sp. Probiotics on the survival rate of white snapper (Lates calcarifer) larvae.  The research was conducted at the Gondol Research Institute for Mariculture, Bali, over a 20-day period using a Completely Randomized Design (CRD) with three treatments and three replications: P0 (control, without probiotics), P1 (3 mg/L), and P2 (5 mg/L). Observed parameters included water quality (nitrate, nitrite, ammonia, phosphate, temperature, pH, salinity, DO), total bacterial count (TBC), survival rate (SR), larval length, and hatching rate (HR). The results showed that the administration of Bacillus sp. probiotics had a significant effect on the survival rate of white snapper larvae (p < 0.05). Based on ANOVA, there were significant differences in SR between treatments. The Bonferroni post hoc test revealed that treatment P2 (5 mg/L) was significantly different from both the control (P0) and P1 (3 mg/L), with P2 producing the highest average SR of 84%, compared to P1 at 70.3% and the control at 50.3%. Meanwhile, probiotic administration did not have a significant effect (p > 0.05) on water quality parameters or larval length. The highest larval length was also recorded in treatment P2, with an average of 1.5 cm on day 20. The use of probiotics contributed to the reduction of toxic compounds such as nitrate, nitrite, and ammonia, and helped stabilize the rearing water environment. Based on these results, it can be concluded that the administration of Bacillus sp. probiotics at a dose of 5 mg/L is the optimal dosage for improving the survival rate of white snapper larvae and maintaining the quality of the aquaculture environment.
EFFECTIVENESS ANALYSIS OF LIQUID ORGANIC FERTILIZER MADE FROM CATFISH (Clarias sp.) VISCERA WASTE ON THE PRODUCTIVITY OF Azolla microphylla Andika Hasyim; Ni Nyoman Dian Martini; Jasmine Masyitha Amelia
Aquasains: Jurnal Ilmu Perikanan dan Sumberdaya Perairan Vol. 14 No. 2 (2026): March 2026
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aqs.v14i2.13280

Abstract

Catfish (Clarias sp.) viscera waste has the potential to pollute the environment but contains high levels of N, P, and K nutrients. This study aimed to determine the effect of liquid organic fertilizer (LOF) made from catfish viscera waste on the productivity of Azolla microphylla and to identify the most effective dosage. A Completely Randomized Design (CRD) with three treatments was used: P0 (control, no LOF), P1 (dose 2.250 ml/L), and P2 (dose 2.625 ml/L), each replicated three times. LOF was produced through 14-day fermentation using Lactobacillus spp. probiotic. Azolla microphylla was cultivated in 25-liter buckets with an initial weight of 30 g for 30 days. Parameters measured included absolute weight growth and daily growth rate. ANOVA results showed significant differences among treatments (p=0.009). Tukey Post Hoc test indicated that P1 and P2 differed significantly from P0 but not from each other. Treatment P1 produced the highest absolute weight growth (203.00 g), followed by P2 (173.33 g) and P0 (108.67 g). A dose of 2.250 ml/L was the most effective dosage to enhance Azolla microphylla productivity.
PERENDAMAN EKSTRAK DAUN JERUJU (Acanthus ilicifolius) UNTUK MENINGKATKAN IMUNITAS DAN SINTASAN UDANG VANAME (Litopenaeus vannamei) YANG TERINFEKSI BAKTERI Vibrio parahaemolyticus: Soaking In Jeruju Leaf Extract (Acanthus ilicifolius) To Enhance Immunity And Survival Rates In Vaname Shrimp (Litopenaeus vannamei) Infected With The Bacterium Vibrio parahaemolyticus Devan Rengga Purwanto; Ni Nyoman Dian Martini; Jasmine Masyitha Amelia
JURNAL AKUAKULTUR, TEKNOLOGI DAN MANAJEMEN PERIKANAN TANGKAP, ILMU KELAUTAN Vol 9 No 1 (2026): JOURNAL OF INDONESIAN TROPICAL FISHERIES
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Muslim Indonesia Makassar, Sulawesi Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/wgsj4s87

Abstract

White shrimp (Litopenaeus vannamei) is a leading aquaculture commodity in Indonesia whose production is often hampered by Vibrio parahaemolyticus infection, the causative agent of Acute Hepatopancreatic Necrosis Disease (AHPND) with mortality reaching 85–100%. This study aimed to determine the effect of immersion in jeruju leaf extract (Acanthus ilicifolius) on the immune response and survival of V. parahaemolyticus-infected white shrimp, and to identify its optimal concentration. A Completely Randomized Design (CRD) was used with five groups: negative control, positive control, and extract immersion at 200, 300, and 400 mg/L for 30 minutes. Data were analyzed using One Way ANOVA followed by Duncan's test. Results showed that jeruju leaf extract immersion significantly affected (p<0.05) THC, DHC composition (an increase in the proportion of granular cells), and post-infection survival rate. The 400 mg/L concentration (treatment C) produced the best results, with the highest THC of 15.08 × 10⁶ cells/ml, the highest proportion of granular cells at 65.67%, and the highest SR of 79.17%, compared to only 48.33% in the positive control. These findings demonstrate that jeruju leaf extract has potential as an environmentally friendly herbal immunostimulant for controlling V. parahaemolyticus infection in white shrimp aquaculture.
Efektivitas Dosis Probiotik EM4 terhadap Pertumbuhan dan Sintasan Benih Ikan Nila (Oreochromis niloticus) Putu Satria Nugraha; Kadek Lila Antara; Jasmine Masyitha Amelia
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 9 No 1 (2026): ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Edisi Mei 2026
Publisher : Cenderawasih University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/acr.v9i1.5314

Abstract

Keberhasilan produksi benih ikan nila (Oreochromis niloticus) pada sistem budidaya intensif sering terkendala oleh rendahnya efisiensi pemanfaatan nutrien dan tingginya kerentanan terhadap stres lingkungan pada fase awal pertumbuhan. Penelitian ini bertujuan mengevaluasi pengaruh pemberian dosis probiotik EM4 yang berbeda dalam pakan terhadap Laju Pertumbuhan Spesifik (Specific Growth Rate/SGR) dan Tingkat Kelangsungan Hidup (Survival Rate/SR) benih ikan nila. Penelitian dilaksanakan selama 30 hari di Balai Benih Ikan (BBI) Ringdikit menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan, yaitu kontrol (0 mL/kg pakan), 10 mL/kg, 20 mL/kg, dan 30 mL/kg pakan. Hasil analisis ragam menunjukkan bahwa suplementasi EM4 berpengaruh signifikan terhadap pertumbuhan benih (P < 0,05). Pertumbuhan optimal diperoleh pada dosis 20 mL/kg pakan dengan nilai SGR bobot sebesar 3,02% dan SGR panjang sebesar 3,46%. Tingkat kelangsungan hidup tidak menunjukkan perbedaan nyata antarperlakuan (P > 0,05), namun secara deskriptif dosis 20 mL/kg menghasilkan sintasan tertinggi (90,00%) dibandingkan kontrol (66,67%). Parameter kualitas air selama pemeliharaan berada dalam kisaran optimal sehingga tidak menjadi faktor pembatas. Berdasarkan hasil penelitian, dosis 20 mL/kg pakan menunjukkan efektivitas tertinggi dalam meningkatkan pertumbuhan serta mempertahankan stabilitas sintasan benih ikan nila.
Struktur Komunitas Terumbu Karang dan Ikan Karang di Pantai Bembeng, Buleleng, Bali Jasmine Masyitha Amelia; I Nyoman Dodik Prasetia; I Gede Iwan Setiabudi
Journal of Marine Research Vol 15, No 3 (2026): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v15i3.58793

Abstract

Pantai Bembeng di Kabupaten Buleleng, Bali merupakan kawasan pesisir yang memiliki ekosistem terumbu karang dan berpotensi mendukung berbagai aktivitas pemanfaatan sumber daya pesisir. Informasi mengenai kondisi terumbu karang dan komunitas ikan karang di kawasan ini masih terbatas sehingga diperlukan kajian sebagai dasar pengelolaan dan pemanfaatan yang berkelanjutan. Penelitian ini bertujuan untuk menganalisis struktur komunitas terumbu karang dan ikan karang di Pantai Bembeng. Pengambilan data dilakukan pada Agustus - Desember 2025 di tiga stasiun pengamatan menggunakan metode Line Intercept Transect (LIT) untuk mengukur tutupan karang dan Underwater Visual Census (UVC) untuk mengamati komunitas ikan karang. Hasil penelitian menunjukkan bahwa tutupan karang hidup tergolong rendah dengan nilai berturut-turut sebesar 8,0%, 23,5%, dan 12,8% pada Stasiun 1, 2, dan 3. Komponen substrat didominasi oleh pasir, batuan vulkanik, dan pecahan karang dengan persentase antara 69,0–90,0%. Sebanyak 38 spesies ikan karang yang tergolong ke dalam 16 famili berhasil diidentifikasi dengan total kelimpahan 587 individu. Famili Pomacentridae dan Acanthuridae merupakan kelompok yang memiliki jumlah individu tertinggi. Meskipun kondisi terumbu karang masih tergolong buruk, keberadaan berbagai kelompok ikan karang menunjukkan bahwa habitat perairan Pantai Bembeng masih mendukung fungsi ekologis ekosistem terumbu karang. Hasil penelitian ini dapat digunakan sebagai informasi dasar dalam upaya pengelolaan, konservasi, dan pemantauan ekosistem terumbu karang di Pantai Bembeng.
EFLUEN TAMBAK UDANG TERHADAP MIKROBIOMA PESISIR DAN IMPLIKASINYA TERHADAP KUALITAS AIR SERTA PROLIFERASI PATOGEN Ni Kadek Martha Ayu Saraswati; Jasmine Masyitha Amelia; Anak Agung Ayu Agung Widhyani; Meutia Samira Ismet; Ketut Mahardika; Gede Iwan Setiabudi
Vol 16 No 4 (2026): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v16i4.2421

Abstract

Shrimp aquaculture faces significant challenges from disease outbreaks and environmental degradation caused by cultivation waste. This study aims to characterize the indicator bacteria communities in the coastal areas surrounding shrimp farms and to evaluate their correlation with water quality parameters as a tool for monitoring ecosystem health. This research employed a descriptive-observational approach and conventional cultivation on selective media, including Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar and Pseudomonas Isolation Agar, to quantify the abundance of Vibrio spp. and Pseudomonas spp., respectively, alongside Marine Agar. Measurements of water quality parameters such as temperature, salinity, pH, dissolved oxygen (DO), nitrate, nitrite, and ammonia were also integrated for correlation analysis. The analysis revealed that aquaculture effluent significantly increased the abundance of total heterotrophic bacteria and Vibrio spp. in the coastal waters receiving farm waste. The abundance of Vibrio was positively correlated with temperature and pH, and negatively correlated with dissolved oxygen. Bacterial communities, including Vibrionaceae and Pseudomonadaceae, were identified as core components of the microbiome in waters exposed to anthropogenic waste. Fluctuations in environmental parameters trigger shifts in these bacterial communities, elevating the risk of pathogen and toxin presence. These findings underscore the importance of monitoring indicator bacteria for sustainable farm management, mitigating disease risks, and ensuring the suitability of coastal waters as a viable medium for aquaculture.