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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Indonesian Tourism and Development Studies Biotropika Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Proceeding International Conference on Global Resource Conservation Natural B Floribunda G-Tech : Jurnal Teknologi Terapan Jurnal Riset Biologi dan Aplikasinya Jurnal Pengabdian Papua Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Rodiyati Azrianingsih
Jurusan Biologi Fakultas MIPA Universitas Brawijaya Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Journal : Natural B

 1 
Variant Clustering Analysis Amorphophallus muelleri Blume Found in East Java Based on Molecular Marking CslA Mannan Synthase Encoder with PCR-RFLP Technique Novie Ary Priyanti; Arik Arubil; Laras Estri Arumningtyas; Rodiyati Azrianingsih
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1397.82 KB) | DOI: 10.21776/ub.natural-b.2013.002.02.4

Abstract

Amorphophallus muelleri Blume is a ubiquotus plant found all over Indonesia and has the potential to be food substitute due to its high glucose content. This research was conducted to investigate the porang’s clustering based on CslA gene glucose coding by using PCR-RFLP technique. Porangs used as the subjet of this study were obtained from 4 different locations in East Java, wich are : (1) Nganjuk, (2) Madiun, (3) Blitar and (4) Jember, analysed by morphological characteristic and molecular traits. Morphological analysis showed that porang derived based on their stalk pattern. BlitarI, JemberI, MadiunI variant have large prismatic stalk patterns, Jember1 and Nganjuk2 have a tight prismatic stalk pattern. Glucomannan content analysis showed that highest content is from Blitar2 5,469% and the lowest is Madiun2 0,2313%. The amplification of CslA gene sequence using primary AkCslA680F and AkCslA1288R showed that CslA gene sequence was amplified on 200 bp. The cutting by using HaeIII restrictive enzyme produced two monomorphic band on 1000 bp and 2000 bp, and 8 polymorphic band on 100 bp-400 bp. The result of this analysis showed that porang had high diversity. The analysis of among variants clustering based on their molecules data showed that grouping fell into 3 clusters, which were: cluster I (JemberI, BlitarI and MadiunI variants), cluster II (Jember2, Blitar2 and Nganjuk2 variants) and cluster III (Blitar2 variant). The shortest distance was found between JemberI and BlitarI variants, while the furthest was found in Blitar2 variants. 
Inventory of Medicinal Plants in Kampung Adat Urug, Urug Village, Sukajaya District, Bogor Regency Muhammad Qais Izzuddin; Rodiyati Azrianingsih
Natural B, Journal of Health and Environmental Sciences Vol 3, No 1 (2015)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.109 KB) | DOI: 10.21776/ub.natural-b.2015.003.01.11

Abstract

This research purposed to find out species, preparation and uses of medicinal plants in Kampung Adat Urug. Ethnobotanical survey was conducted from November 2013 until May 2014 using interview (semi-structured) and observation activities. The respondents were determinated by snowball sampling with the head of Kampung Adat Urug as a key informant. The observation was conducted for documenting medicinal plants based on respondent recomendation. Data gained from this activity was qualitatively described. Medicinal plants used by the Kampung Urug were 53 species singly and 5 herbs. Those species belonged 27 families. The most recomended medicinal plants were Cardamom and Bitung Bamboo. Those plants often to treat cough with dringking extraxted water from trunk (dituak). Bitung Bamboo shoot soup is used to prevent aging. In Kampung Adat Urug, traditional midwife often use medicinal plants to make herbs for healing any diseases, such as headache, stomachache, kidney problem, and also increasing stamina for adult and women after pregnant. Medicinal plants and herbs which were recomended by respondents were adequate treatment for any diseases because they have evidences between ethic and emic  
Co-Authors Aini, Afifah Nur Amin Setyo Leksono Anggraeni, Wahyu Ririn Arik Arubil Bagyo Yanuwiadi Didik Wahyudi Dwi Anggorowati Rahayu Dwi Gusmalawati Endik Deni Nugroho Estri Laras Arumingtyas Fajarini, Nindi Nazula Filza Yulina Ade Gustini Ekowati Haryati, Jehan Ramdani Hendrian Hendrian, Hendrian Herawati Herawati Ikbal, Mohammad Irfan Mustafa Isna Arofatun Nikmah, Isna Arofatun Jamhari Jamhari Laras Estri Arumningtyas Luchman Hakim Moh. Ihsan Muhammad Efendi, Muhammad Muhammad Qais Izzuddin Natalia, Yessica Kristi Nia Kurniawan Novie Ary Priyanti Nunung Harijati Padmana, I Made Dhena Radya Qurotaayuina, Riska Putri Rachma, Risca Adiyani Rugayah Rugayah Sari, Widya Artika Septiani, Yuni Serafinah Indriyani Serafinah Indriyani Serafinah Indriyani Serafinah Indriyani Sri Widyarti Sundari Sundari Turhadi Turhadi Zainal Abidin Zainal Abidin