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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Indonesian Tourism and Development Studies Biotropika Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Proceeding International Conference on Global Resource Conservation Natural B Floribunda G-Tech : Jurnal Teknologi Terapan Jurnal Riset Biologi dan Aplikasinya Jurnal Pengabdian Papua Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Rodiyati Azrianingsih
Jurusan Biologi Fakultas MIPA Universitas Brawijaya Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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The Potency of Trichoderma sp. as A Biocontrol Agent against Fusarium sp. Pathogen of Porang (Amorphophallus muelleri Blume) Tuber Fajarini, Nindi Nazula; Azrianingsih, Rodiyati; Suharjono, Suharjono
Journal of Tropical Life Science Vol 10, No 1 (2020)
Publisher : Journal of Tropical Life Science

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Abstract

Porang (Amorphophallus muelleri Blume) has high economic value in Asia. Porang tuber is high in glucomannan and thus becomes one of the exportable food commodities. Porang plants are susceptible to wilt disease caused by Fusarium sp. Trichoderma sp. has the ability to inhibit the growth of pathogenic fungi such as Fusarium sp. This research aimed to study the potency of Trichoderma sp. in controlling the growth of Fusarium sp. Trichoderma sp. was isolated from the rhizosphere soil in A. muelleri plantation, while Fusarium sp. was isolated from A. muelleri tuber infected by Fusarium. The fungi isolates were identified phylogenetically based on the similarity of Internal Transcribed Spacers (ITS) sequence. Both fungi were antagonistically assessed based on the dual culture method. The antagonistic assay showed that the two isolates of Trichoderma had the potency to inhibit the growth of the two isolates of Fusarium. Trichoderma sp. 2 has higher antagonistic potency than Trichoderma sp. 1. Based on ITS sequence similarity, Trichoderma sp. 1 and Trichoderma sp. 2 were identified as Trichoderma longipale and Trichoderma spirale respectively, while both pathogenic Fusarium were identified as Fusarium oxysporum. Trichoderma spirale therefore could be developed as a biopesticide agent in controlling Fusarium oxysporum.
Identification of Potential Pathogen Bacteria Causing Tuber Rot in Porang (Amorphophallus muelleri Blume) Aini, Afifah Nur; Azrianingsih, Rodiyati; Mustafa, Irfan
Journal of Tropical Life Science Vol 10, No 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.245 KB) | DOI: 10.11594/jtls.10.02.02

Abstract

Tuber rot has been reported as one of the limiting factors to porang (Amorphophallus muelleri Blume) tuber production. Bacterial infection is one of the main causes of tuber rot during storage. In this study, a total of seven bacterial isolates obtained from porang tubers were tested for their potency to cause rotting on porang tubers. Porang tubers were immersed in bacterial suspension and incubated for 14 days. The physical properties of the tubers such as texture and color, and the chemical properties such as reducing sugar and ethanol content were determined at the end of incubation period. All infected tubers were soft, slimy and darker. Two isolates, T4 and T9A, had the highest potency to cause tuber rot indicated by the significantly higher reducing sugar content (14.38 and 15.32 mg/ml) and higher ethanol content (32.17 and 35.05%) compared to control tubers which were not infected with bacteria. Both isolates were identified as Bacillus altitudinis and Pseudomonas stutzeri, respectively, based on 16S rDNA sequence analysis.
Genetic Diversity of Porang Populations (Amorphophallus Muelleri Blume) In Central Java and West Java Based on LEAFY Second Intron Marker Nikmah, Isna Arofatun; Azrianingsih, Rodiyati; Wahyudi, Didik
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.05

Abstract

Porang (Amorphophallus muelleri Blume) dispersed and grows well in Java island. This research aimed to determine the genetic diversity of porang populations in Central Java and West Java based on LEAFY second intron (nuclear genome encoding flower development). DNA samples of porang were from previous study, which are obtained from Central Java (Pamedaran, Grobogan, Wonogiri, Karangtengah) and West Java (Cisompet) as ingroup. A. variabilis from Pamedaran Brebes, A. ochroleucus, A. longituberosus, A. sumawongii, and A. tinekeae from genbank were treated as outgroup. LEAFY second intron was amplified using primers FLint2 F1 5’-CTTCCACCTCTACGACCAGTG-3’ and FLint2 R1 5’-TCTTGGGCTTGTTGATGTAGC-3’. Analysis using dnasp5 showed that 23 samples had 89 invariable sites and 139 variable sites that consisted of 12 singleton two variants sites, 118 parsimony-informative two variants sites and 9 Parsimony-informative three variants sites. The total of haplotype were 7 haplotypes where 16 ingroup samples clumped on haplotype 1 whereas 2 samples of Grobogan (Grobogan I and Grobogan III) clumped on haplotype 2 and outgroup samples occupied at their own haplotype. Genetic distance of all ingroup samples were 0-0,004 showing no differences among individuals. Analysis of phylogenetic tree using Maximum Parsimony, Neighbor Joining and Maximum Likelihood resulting a polytomy branch of ingroup samples except Grobogan which forms a separated sub clad. Key words: Genetic diversity, haplotype, Java, LEAFY, Porang.
Ethno-Edible Mushroom of Pleurotus sp., Clytocybe nebularis and Auricularia auricula in Ranupani Village, East Java Haryati, Jehan Ramdani; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 2, No 2 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

This paper documented the knowledge about the wild edible fungal flora of Ranupani Village of East Java Indonesia that used by indigenous Tenggerese tribes. Study was conducted by using semi-structured interview and purposive sampling. Data were analyzed quantitatively descriptive. Index of Cultural Significance (ICS) was used to evaluate the importance of non-wood forest yields for local people. Identification of edible mushrooms was based on Guidelines of Flora Diversity Data Collection. This study was focus on the three edible mushrooms which are eaten mostly by the villagers, i.e. Pleurotus sp., Clytocybe nebularis and Auricularia auricula. The result is the Ranupani residents perception of edible mushrooms and its potential as an alternative source of food based on local knowledge and local wisdom of Tenggerese. Pleurotus sp. has the highest preference and intensity to be consumed with 3,2 ICS. It means culturally, this edible mushroom were not too important but intensively used as a secondary food sources such as soup (added or sauted), chips (dried) and fried. Ranupani Villagers always consumed edible mushrooms in rainy season. The ability of residents to distinguish edible mushrooms are equal to the searching ability to find edible mushroom substrate. The residents will recognized the substrate of edible mushrooms on dead trunks of Pasang Tree (Lithocarpus sundaicus), Danglu (Engelhardia spicata), Kemlandingan (Albizia Montana), Casuarina (Casuarina junghuhniana) and acacia (Acacia decurens). Residents who do not have the ability to distinguish and searching were consumer, get the edible mushrooms by buying from the searcher or distributor in packs. The characteristics of an edible mushrooms are a discrete soft flesh of the fruit body, dark color, no ring on the stipe, the presence of insects (e.g. moths) in the lamellae) and the type of mushroom substrate. There were also assisted growths of wild mushrooms by the residents’ raw chop the dead tree trunk as a substrate deliberately. Respondents who knew the time of harvesting edible mushroom were 58.3%. Pasang mushrooms (natural & assisted growth) can be found throughout the rainy season, with an interval of 12 days for subsequent retrieval. While Barat and Kuping mushrooms are found throughout the rainy season, but randomly distributed in the forest. There were unwritten but spoken hereditary local wisdom for the sustainable of the edible mushrooms in Tengger. Only Tenggerese allowed collecting and selling the edible mushrooms in Tengger area to the Tenggerese themselves and tourist (local or foreign). This wisdom is supported by TNBTS regulation that the edible mushroom were not allowed exported out of TNBTS area. This wisdom expectedly makes sure that the edible mushrooms should be sustained in the forest. Keywords:Ethno-edible mushrooms, Ranupani
Effect of Dietary Amorphophallus sp From East Java on LDL-C Rats (Rattus novergicus Wistar Strain) Harijati, Nunung; Widyarti, Sri; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 1, No 2 (2011)
Publisher : Journal of Tropical Life Science

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Abstract

One of indication of obesity is high LDL-C . Obesity has serious risk to health, it can cause heart disease and stroke. Effort to lower obesity using drugs have significant side effects such as insomnia, increased blood pressure, dry mouth and so forth. Therefore using natural products that contain glucomannan to reduce obesity and LDL-C is good choise. Glucomannan in the global market derived from Amorphophallus konjac. In this study, we used glucomannan from Amorphophallus endemic East Java to reduce fattened Wistar rats. Amorphophallus that used include Amorphophallus muelleri, A.variabilis (variant: Brongkos 32; Brongkos 5; Wonorejo, Brangsi) and Amorphophallus campanulatus (Var Selopuro). Amorphophallus diet was given to white rats of Wistar strain that previously fattened using a mixture of cholesterol and lard, in addition to PARS (Chicken Feed Race Super) as a basic food. Amorphophallus konjac was used as control. Measurement of LDL-C was conducted in four different time points, namely 1). Prior to dietary cholesterol, 2). 24 days after the dietary cholesterol, 3) 11 days after Amorphophallus (+cholesterol) diet, 4). 25 days after Amorphophallus (+cholesterol) diet. The results showed that the diet of A.variabilis potentially lowered blood cholesterol levels for their respective 22.98%, 5.85% and 7.37% for consecutive variant Brongkos 32; Brongkos 5; Wonorejo. Diet from A.campanulatus and A.konjac had not been able to reduce cholesterol to the end of observation (25 days).Keywords: LDL-C, A.variabilis, A.muelleri, A.campanulatus, A.konjac
Conservation strategy of Anaphalis spp. in Bromo Tengger Semeru National Park, East Java Ade, Filza Yulina; Hakim, Luchman; Arumingtyas, Estri Laras; Azrianingsih, Rodiyati
Journal of Tropical Life Science Vol 11, No 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/tls.11.01.10

Abstract

The objective of the paper were to identify factors related to conservation strategy of Anaphalis in BTSNP. This study found internal factor related to strength includes the existence of species and value of Anaphalis spp., while the weaknesses include increase of habitat degradation and disturbance caused by tourism activities. The opportunities for conservation includes support of policy in biodiversity conservation and increase of ecotourism movement, while the threats aspect include less control of land uses changes and increase of mass tourism. The SWOT analyses recommends some significant strategy which are derived from combination of enhancing strength to optimizing opportunities, reducing weaknesses and optimizing opportunities, Enhancing strength to reduce threats and managing weaknesses to manage risk.  
Co-Authors Aini, Afifah Nur Amin Setyo Leksono Anggraeni, Wahyu Ririn Arik Arubil Bagyo Yanuwiadi Didik Wahyudi Dwi Anggorowati Rahayu Dwi Gusmalawati Endik Deni Nugroho Estri Laras Arumingtyas Fajarini, Nindi Nazula Filza Yulina Ade Gustini Ekowati Haryati, Jehan Ramdani Hendrian Hendrian, Hendrian Herawati Herawati Ikbal, Mohammad Irfan Mustafa Isna Arofatun Nikmah, Isna Arofatun Jamhari Jamhari Laras Estri Arumningtyas Luchman Hakim Moh. Ihsan Muhammad Efendi, Muhammad Muhammad Qais Izzuddin Natalia, Yessica Kristi Nia Kurniawan Novie Ary Priyanti Nunung Harijati Padmana, I Made Dhena Radya Qurotaayuina, Riska Putri Rachma, Risca Adiyani Rugayah Rugayah Sari, Widya Artika Septiani, Yuni Serafinah Indriyani Serafinah Indriyani Serafinah Indriyani Serafinah Indriyani Sri Widyarti Sundari Sundari Turhadi Turhadi Zainal Abidin Zainal Abidin