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Memfasilitasi Pelajar KIR (Karya Ilmiah Remaja) Biologi Dengan Kajian Isolasi dan Identifikasi Bakteri Escherichia coli Dari Sampel Kotoran Hewan Ternak Sepanjang SMAN 5 Cilegon Di Laboratorium UPTD Banten Rezaldi, Firman; Cahyono, Andri Tri; Wati, Dyah Rohma; Ginaris, Rifkarosita Putri; Utami, Arti Wahyu; Wijayanti, Fitria Eka Resti; Purbanova, Reni; Nabilla, Jihan; Nurfadhillah, Dea; Faizah, Na’imatul Retno; Nurrahman, Alfina; Oentari, Octavina Dyah; Pramono, Edi; Yudianto, Tri; Sari, Sinta Wisma; Widyaningsih, Ragil; Kustyarini, Farida; Fatchani, Sathi
Jurnal Pengabdian dan Pengembangan Masyarakat Indonesia Vol. 3 No. 2 (2024)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jppmi.v3i2.275

Abstract

Cemaran bakteri Escherichia .coli merupakan salah satu ancaman bagi setiap daya tahan tubuh makhluk hidup. Menurunnya daya tahan tubuh makhluk hidup disebabkan oleh minimnya kualitas kesehatan yang bersumber pada produk makanan yang tercemar, produk obat yang tercemar, produk kosmetik yang tercemar hingga lingkungan sekitar yang sering tercemar berat. Lingkungan sekitar yang sering tercemar cukup tinggi dan berpeluang besar oleh adanya serangan bakteri Escherichia. coli terdiri atas produk hasil pertanian maupun peternakan yang banyak menimbulkan diare bagi masyarakat yang tidak memiliki imunitas yang kurang stabil. Aktivitas peternakan menjadi salah satu permasalahan dalam mencemari lingkungan adalah kotoran hewan ternak disepanjang SMAN 5 Cilegon. Kotoran hewan ternak disepanjang SMAN 5 Cilegon terdiri dari kotoran hewan ternak ayam, kambing, dan kerbau. Ketiga kotoran hewan ternak tersebut tentunya mengandung bakteri Escherichia coli yang merupakan salah satu bakteri gram negatif penyebab kejadian luar biasa atau KLB yang perlu diberikan suatu pemaparan bagi siswa maupun siswi SMAN 5 Cilegon yang pro aktif dalam mengikuti Karya Ilmiah Remaja (KIR) bidang Biologi untuk meningktakan pengetahuan dan wawasan mengenai keberadaan bakteri E coli yang terdapat di SMAN 5 Cilegon dari tiga kotoran hewan ternak. Pengabdian kepada masyarakat ini adalah bertujuan untuk memfasilitasi siswa maupun siswi yang terlibat dalam kegiatan KIR Biologi untuk memperoleh cara isolasi dan identifikasi bakteri Escherichia coli yang terdapat di sepanjang SMAN 5 Cilegon Banten. Pengabdian kepada masyarakat bekerja sama dengan Laboratorium UPTD Pengujian dan Penerapan Mutu Hasil Perikanan (PPMHP) Provinsi Banten dengan metode yang berlaku. Tahapan kegiatan ini meliputi survey, pra plaksanaan, pelaksanaan, dan evaluasi. responden yang telah teruji berdasarkan hasil pre test mengenai wawasan dan pengetahuan isolasi dan identifikasi bakteri E. coli yang diambil dari tiga sampel kotoran hewan ternak sepanjang SMAN 5 Cilegon. Siswa siswi yang paham sebesar 23% dan 77% adalah komposisi persentase siswa dan siswi yang belum paham mengenai materi isolasi bakteri E. coli dari ketiga sampel kotoran hewan ternak. Sampel kotoran hewan ternak yang terdapat disepanjang SMAN 5 Cilegon seperti kerbau, kambing, dan ayam telah terbukti mengandung bakteri Escherichia coli
AKTIVITAS ANTIOKSIDAN PADA SEDIAAN KOMBUCHA BUNGA TELANG YANG DIRACIK DARI FORMULASI GULA AREN DAN MADU SR12 SEBAGAI PRODUK BIOTEKNOLOGI PANGAN DAN FARMASI Rezaldi, Firman; Utami, Arti Wahyu; Resti Wijayanti, Fitria Eka; Purbanova, Reni; Wati, Dyah Rohma; Suminar, Erny; Kusumiyati, Kusumiyati; Mu’jijah, Mu’jijah; Faizah, Nai’matul Retno; Cahyono, Andri Tri; Setyaji, Diyan Yunanto; Fadillah, M Fariz; Yenny, Ratna Fitry
AGRIBIOS Vol 22 No 2 (2024): November
Publisher : Program Studi Agribisnis Fakultas Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/agribios.v22i2.5209

Abstract

The food and pharmaceutical fields are two elements needed in everyday life to improve human health and well-being. This is because functional foods, medicines, and natural cosmetics have become an increasingly current trend to be applied both based on food biotechnology and pharmaceutical biotechnology. One of the food and pharmaceutical biotechnology products that can be effective as a source of antioxidants is the butterfly pea flower kombucha preparation. Butterfly pea flower kombucha preparations that are effective as a source of antioxidants certainly have a substrate formula that varies based on the results of their concoctions. The results of previous studies have proven that butterfly pea flower kombucha with a concentration of palm sugar and SR12 honey has activity as a source of antibacterial. Research to see the antioxidant activity that is formulated based on various formulations of palm sugar and SR12 honey substrates. The stages of this research include making butterfly pea flower kombucha with various concentrations of palm sugar and SR12 honey substrates and testing its antioxidant activity on each preparation formulated from the palm sugar and SR12 honey substrate formulation (20%, 30%, and 40%). One-way analysis of variance was applied, followed by post-hoc analysis. Based on the post-hoc analysis, the study showed that butterfly pea flower kombucha at a concentration of 40%, as well as the formula of palm sugar and SR12 honey, showed significant differences from the concentrations of 20% and 30% after testing one-way analysis of variance, with each P value <0.05. The conclusion is that the preparation of butterfly pea flower kombucha based on the use of palm sugar and SR12 honey substrates is efficacious as a source of antioxidants so that in the long term it can be used as a functional food ingredient or pharmaceutical.
SOSIALISASI MENGENAI POTENSI PRODUK BIOTEKNOLOGI FARMASI BERUPA FORMULASI DAN SEDIAAN SPRAY KOMBUCHA BUNGA TELANG DAN BIOTEKNOLOGI PERTANIAN BERUPA PUPUK CAIR ORGANIK DARI LIMBAH FERMENTASI KOMBUCHA BUNGA TELANG KEPADA PETANI HORTIKULTURA K.P PEKUNCEN Rezaldi, Firman; Utami, Arti Wahyu; Sari, Sinta Wisma; Faizah, Nai’matul Retno; Wati, Dyah Rohma; Resti Wijayanti, Fitria Eka; Ginaris, Rifkarosita Putri; Purbanova, Reni; Sugiono, Sugiono; Saifullah, Ipul; Rohmatulloh, Rizal; Kurniawan, Maskun; Munir, Misbakhul; Mu'jijah, Mu'jijah; Sasmita, Heny; Somantri, Ucu Wandi
PARADIGMA PENGABDIAN Vol 1 No 1 (2024): Edisi : November
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/paradigma.v1i1.5270

Abstract

Horticultural commodity crops are commodities that have high selling value and have properties as functional food and pharmaceutical ingredients. So the majority of horticultural farmers often prioritize meeting market needs. Obstacles that occur in the field are decreasing quality and quantity due to pest and disease attacks as well as excessive use of chemical-based fertilizers. The aim that can be offered in this community service activity is to provide education about the potential of butterfly pea flower kombucha, apart from being used as a functional food, it can also be used as a natural antibiotic without other additives as an antifungal pathogen from horticultural commodity plants as well as providing education about the potential of butterfly pea flower kombucha as a raw material for organic liquid fertilizer to support the growth of horticultural commodity plants which has been proven by various research results. by providing an explanation of the basics of butterfly pea flower kombucha fermentation which is useful as a probiotic drink to enhance the immune system in general which has a high potential for being a functional food, pharmaceutical/medicinal or cosmetic, an ingredient in organic liquid fertilizer preparations, and a natural antibiotic for fungal pathogens of horticultural plants. Horticultural farmers from Pekuncen village learned about the potential of butterfly pea flower kombucha with a percentage of 100% after the presentation of the material.
Isolation and Characterization of Endophytic Bacteria from Curcuma Aeruginosa Roxb As Antibacterial Candidates Againts Pneumonia Utami, Arti Wahyu; Rezaldi, Firman; Ginaris, Rifkarosita Putri; Nabilla, Jihan; Bramantyo; Setiawati, Puput; Fatikha, Zulfania Shandra; Pujiastuti, Nanik; Wahyuni, Dwi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18148

Abstract

This study aimed to isolate and characterize endophytic bacteria from black turmeric rhizomes (Curcuma aeruginosa Roxb) and test their potential as antibacterial agents against Streptococcus pneumoniae. This experimental research began with the isolation of endophytic bacteria from surface-sterilized black turmeric rhizomes. The obtained pure isolates were characterized macroscopically (colony shape, margin, elevation, pigment) and microscopically (cell shape, Gram staining). The growth curve of potential isolates was determined, and antibacterial activity was tested using the disc diffusion method against S. pneumoniae, with ciprofloxacin as a positive control. Six endophytic bacterial isolates (PS1, PS2, PS3, PS4, PS5, PS6) were successfully isolated, showing variations in colony morphology, and all were classified as Gram-negative bacilli. All isolates exhibited strong antibacterial activity against Streptococus pneumoniae, with inhibition zone diameters ranging from 15.3 mm to 20.6 mm. Black turmeric rhizomes (Curcuma aeruginosa Roxb) harbor diverse endophytic bacteria with significant potential as antibacterial agents against the pneumonia pathogen, Streptococcus pneumoniae.
AKSI NYATA PENGABDIAN CIVITAS AKADEMIK KEPADA WARGA KARANGANYAR MELALUI DONOR DARAH GRATIS DALAM RANGKA DIES NATALIS STIKES TUJUH BELAS KE-6 Rizaldi, Firman; Utami, Arti Wahyu; Dyah Oentari, Octaviana; Nabilla, Jihan; Rahmad, Sukardi Sugeng
PARADIGMA PENGABDIAN Vol 2 No 3 (2025): Edisi: November
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/paradigma.v2i3.7679

Abstract

Kegiatan pengabdian masyarakat berupa donor darah gratis yang dilaksanakan oleh civitas akademika STIKes Tujuh Belas Karanganyar bertujuan untuk mendukung ketersediaan stok darah lokal sekaligus meningkatkan kesadaran masyarakat mengenai pentingnya donor darah sukarela. Pelaksanaan kegiatan ini menggunakan pendekatan partisipatif dengan melibatkan dosen, mahasiswa, tenaga kependidikan, PMI Kota Solo, dan warga Karanganyar sebagai mitra aktif. Kegiatan donor darah berlangsung pada 7 Agustus 2025 di Ruang Rapat Dosen STIKes Tujuh Belas Karanganyar, diawali dengan tahap persiapan berupa koordinasi teknis, penyusunan alur pelayanan, sosialisasi, serta penyediaan logistik. Hasil skrining menunjukkan bahwa 80% peserta memenuhi syarat mendonorkan darah, menghasilkan total 56 kantong darah dengan variasi golongan darah A, B, AB, dan O. Jumlah tersebut memberikan kontribusi nyata terhadap pemenuhan kebutuhan darah PMI Kabupaten Karanganyar dan mencerminkan antusiasme serta kondisi kesehatan calon pendonor yang baik. Kegiatan ini juga memperkuat peran institusi pendidikan dalam meningkatkan literasi kesehatan melalui edukasi publik serta kolaborasi lintas sektor. Secara keseluruhan, program ini terbukti efektif sebagai bentuk aksi nyata kampus dalam mendukung pelayanan transfusi darah serta sebagai strategi berkelanjutan dalam membangun budaya donor darah di masyarakat.