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Memfasilitasi Pelajar KIR (Karya Ilmiah Remaja) Biologi Dengan Kajian Isolasi dan Identifikasi Bakteri Escherichia coli Dari Sampel Kotoran Hewan Ternak Sepanjang SMAN 5 Cilegon Di Laboratorium UPTD Banten Rezaldi, Firman; Cahyono, Andri Tri; Wati, Dyah Rohma; Ginaris, Rifkarosita Putri; Utami, Arti Wahyu; Wijayanti, Fitria Eka Resti; Purbanova, Reni; Nabilla, Jihan; Nurfadhillah, Dea; Faizah, Na’imatul Retno; Nurrahman, Alfina; Oentari, Octavina Dyah; Pramono, Edi; Yudianto, Tri; Sari, Sinta Wisma; Widyaningsih, Ragil; Kustyarini, Farida; Fatchani, Sathi
Jurnal Pengabdian dan Pengembangan Masyarakat Indonesia Vol. 3 No. 2 (2024)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jppmi.v3i2.275

Abstract

Cemaran bakteri Escherichia .coli merupakan salah satu ancaman bagi setiap daya tahan tubuh makhluk hidup. Menurunnya daya tahan tubuh makhluk hidup disebabkan oleh minimnya kualitas kesehatan yang bersumber pada produk makanan yang tercemar, produk obat yang tercemar, produk kosmetik yang tercemar hingga lingkungan sekitar yang sering tercemar berat. Lingkungan sekitar yang sering tercemar cukup tinggi dan berpeluang besar oleh adanya serangan bakteri Escherichia. coli terdiri atas produk hasil pertanian maupun peternakan yang banyak menimbulkan diare bagi masyarakat yang tidak memiliki imunitas yang kurang stabil. Aktivitas peternakan menjadi salah satu permasalahan dalam mencemari lingkungan adalah kotoran hewan ternak disepanjang SMAN 5 Cilegon. Kotoran hewan ternak disepanjang SMAN 5 Cilegon terdiri dari kotoran hewan ternak ayam, kambing, dan kerbau. Ketiga kotoran hewan ternak tersebut tentunya mengandung bakteri Escherichia coli yang merupakan salah satu bakteri gram negatif penyebab kejadian luar biasa atau KLB yang perlu diberikan suatu pemaparan bagi siswa maupun siswi SMAN 5 Cilegon yang pro aktif dalam mengikuti Karya Ilmiah Remaja (KIR) bidang Biologi untuk meningktakan pengetahuan dan wawasan mengenai keberadaan bakteri E coli yang terdapat di SMAN 5 Cilegon dari tiga kotoran hewan ternak. Pengabdian kepada masyarakat ini adalah bertujuan untuk memfasilitasi siswa maupun siswi yang terlibat dalam kegiatan KIR Biologi untuk memperoleh cara isolasi dan identifikasi bakteri Escherichia coli yang terdapat di sepanjang SMAN 5 Cilegon Banten. Pengabdian kepada masyarakat bekerja sama dengan Laboratorium UPTD Pengujian dan Penerapan Mutu Hasil Perikanan (PPMHP) Provinsi Banten dengan metode yang berlaku. Tahapan kegiatan ini meliputi survey, pra plaksanaan, pelaksanaan, dan evaluasi. responden yang telah teruji berdasarkan hasil pre test mengenai wawasan dan pengetahuan isolasi dan identifikasi bakteri E. coli yang diambil dari tiga sampel kotoran hewan ternak sepanjang SMAN 5 Cilegon. Siswa siswi yang paham sebesar 23% dan 77% adalah komposisi persentase siswa dan siswi yang belum paham mengenai materi isolasi bakteri E. coli dari ketiga sampel kotoran hewan ternak. Sampel kotoran hewan ternak yang terdapat disepanjang SMAN 5 Cilegon seperti kerbau, kambing, dan ayam telah terbukti mengandung bakteri Escherichia coli
The Effect of Substituting Wheat Flour with Composite Flour (Cucurbita moschata and Daucus carota L.) on Biscuits as a Source of Beta Carotene for Stunted Children Riana, Asysyifa; Hariadi, Hari; Widiastuti, Yuliati; Harun, Iriyanti; Irawan, Galuh Chandra; Tantie, Lanny; Anzani, Kartika; Rezaldi, Firman; Cahyono, Andri Tri
Biotik Vol 12 No 2 (2024): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v12i2.25279

Abstract

The problem of stunting in Indonesia is still quite high, namely 21,6% based on data from the 2022 Indonesian Nutrition Status Survey (SSGI). One of the effective measures to control stunting is through providing additional food (PMT) such as biscuits to children. Carrots and pumpkin are local foods that are rich in beta carotene. Carotenoids as a source of provitamin A are able to overcome the problem of vitamin A deficiency (VAD), which is the cause of growth disorders in children. This research aims to determine the effect of substitution of yellow pumpkin (Cucurbita moschata) and carrot (Daucus carota L.) composite flour on organoleptic characteristics including color, aroma, taste, texture and nutritional content (beta carotene, carbohydrates, protein and fat). This research used a Randomized Block Design (RAK) with 3 treatments, namely formula 1 (150 grams of wheat flour, 50 grams of pumpkin flour and 50 grams of carrot flour), formula 2 (50 grams of wheat flour, 60 grams of pumpkin flour and 50 grams of flour carrots) and formula 3 (50 grams of wheat flour, 50 grams of pumpkin flour and 150 grams of carrot flour) with 3 repetitions. The research results showed that formula 3 biscuits were the best formulation in terms of organoleptic characteristics with the highest beta carotene content compared to F1 and F2. F3 biscuits contain 30,97 mg beta carotene, 7,45 grams of carbohydrates, 3,22 grams of protein and 8,89 grams of fat. The conclusion of this research is that the substitution of wheat flour with composite flour (Cucurbita moschata and Daucus carota L.) has an effect on the nutritional content (beta carotene, carbohydrates, protein and fat) of biscuits. Consuming pumpkin biscuits per 100 grams can qualify as a good source of the antioxidant beta carotene.
AKTIVITAS ANTIOKSIDAN PADA SEDIAAN KOMBUCHA BUNGA TELANG YANG DIRACIK DARI FORMULASI GULA AREN DAN MADU SR12 SEBAGAI PRODUK BIOTEKNOLOGI PANGAN DAN FARMASI Rezaldi, Firman; Utami, Arti Wahyu; Resti Wijayanti, Fitria Eka; Purbanova, Reni; Wati, Dyah Rohma; Suminar, Erny; Kusumiyati, Kusumiyati; Mu’jijah, Mu’jijah; Faizah, Nai’matul Retno; Cahyono, Andri Tri; Setyaji, Diyan Yunanto; Fadillah, M Fariz; Yenny, Ratna Fitry
AGRIBIOS Vol 22 No 2 (2024): November
Publisher : Program Studi Agribisnis Fakultas Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/agribios.v22i2.5209

Abstract

The food and pharmaceutical fields are two elements needed in everyday life to improve human health and well-being. This is because functional foods, medicines, and natural cosmetics have become an increasingly current trend to be applied both based on food biotechnology and pharmaceutical biotechnology. One of the food and pharmaceutical biotechnology products that can be effective as a source of antioxidants is the butterfly pea flower kombucha preparation. Butterfly pea flower kombucha preparations that are effective as a source of antioxidants certainly have a substrate formula that varies based on the results of their concoctions. The results of previous studies have proven that butterfly pea flower kombucha with a concentration of palm sugar and SR12 honey has activity as a source of antibacterial. Research to see the antioxidant activity that is formulated based on various formulations of palm sugar and SR12 honey substrates. The stages of this research include making butterfly pea flower kombucha with various concentrations of palm sugar and SR12 honey substrates and testing its antioxidant activity on each preparation formulated from the palm sugar and SR12 honey substrate formulation (20%, 30%, and 40%). One-way analysis of variance was applied, followed by post-hoc analysis. Based on the post-hoc analysis, the study showed that butterfly pea flower kombucha at a concentration of 40%, as well as the formula of palm sugar and SR12 honey, showed significant differences from the concentrations of 20% and 30% after testing one-way analysis of variance, with each P value <0.05. The conclusion is that the preparation of butterfly pea flower kombucha based on the use of palm sugar and SR12 honey substrates is efficacious as a source of antioxidants so that in the long term it can be used as a functional food ingredient or pharmaceutical.