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All Journal Indonesian Journal of Tropical and Infectious Disease Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY Jurnal Kimia Riset Sari Pediatri Paediatrica Indonesiana Folia Medica Indonesiana

Soegeng Soegijanto

Universitas Airlangga

Author-ID : 480895

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia Shifa Fauziyah; Sri Subekti; Budi Utomo; Teguh Hari Sucipto; Hebert Adrianto; Aryati Aryati; Puspa Wardhani; Soegeng Soegijanto
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.65357

An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F1534C) alleles in both two species. All of mosquito samples have wild type genotype of kdr alleles (V1016V and F1534F). Data were analysed using R Studio 1.4 Version by Genetics package. Results showed that the frequency of resistant alleles (G1016 and C1534) was zero, and the frequency of susceptible allele was 1 (V1016 and F1534). Insecticide bioassay could not be established due to the limited number of adult mosquitoes, so insecticide resistance status could not be determined. However, this study can be used as preliminary monitoring for the vector control program.

RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol. Yovilianda Maulitiva Untoro; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Shuhai Ueda; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 1 (2018): Juni
Publisher : Universitas Airlangga

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol can used as precipitation solvent for isolating RNA.

Precipitation Solvents for RNA Extraction of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, and Ultrapure H2O Rizqidhana Juliana Putri; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results.

Uji Coba Vaksin Dengue Rekombinan pada Hewan Coba Mencit,Tikus, Kelinci dan Monyet Soegeng Soegijanto; Fedik A Rantam; Soetjipto Soetjipto; Ketut Sudiana; Yoes Priyatna
Sari Pediatri Vol 5, No 2 (2003)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Pencegahan terhadap infeksi dengue dengan cara vaksinasi perlu dikembangkan; olehkarena secara epidemiologi infeksi virus dengue telah menyebar ke daratan Asia, Afrika,Amerika dan Eropa. Pendekatan pencegahan dan pemberantasan dengan melakukanpemberantasan vektor tidaklah cukup untuk menekan angka kesakitan. Pengembanganvaksin dengan menggunakan protein E dapat menginduksi produksi antibodi terhadapsemua strain (galur) virus dengue.Tujuan penelitian menentukan daya proteksi antibodi yang dipacu oleh calon vaksinpada hewan percobaan (mencit, tikus, kelinci dan monyet).Metode penelitian Isolasi virus dari pasien DBD di RS Dr. Sutomo Surabaya dan isolatstandar dari NAMRU-2 Jakarta. Dilakukan purifikasi isolat virus dengue dan purifikasiprotein E rekombinan. Selanjutnya dilakukan imunisasi pada binatang percobaan dandinilai respon imunnya.Hasil penelitian karakterisasi dan identifikasi imunoglobulin dari mencit yang diimunisasidengan protein E selain IgM, IgG ditentukan subklas IgG1a, IgG2a, IgG2b. Protein Epada hewan percobaan dapat menginduksi antibodi humoral dengan berbagai kelasimunoglobulin maupun subkelasnya dan antibodi seluler yang protektif. Analisis hasilrespon imun pada CD 4 dan CD 8 yang di isolasi dari hewan percobaan yang telahdiimunisasi, direuksikan dengan IFN-Â¶ ternyata menunjukkan adanya perbedaan responimun yang berbeda. Pada challenge test hanya monyet yang memberikan respons patologisyaitu terlihat adanya perdarahan pada hari ke tiga setelah infeksi. Protein E yangdiimunisasikan pada monyet dapat menginduksi antibodi humoral dengan titer cukuptinggi terutama imunoglobulin G.

Comparison of the efficacy and safety of hydroxyethyl starch 130/0.4 and Ringer's lactate in children with grade III dengue hemorrhagic fever Risky Vitria Prasetyo; Abdul Latief Azis; Soegeng Soegijanto
Paediatrica Indonesiana Vol 49 No 2 (2009): March 2009
Publisher : Indonesian Pediatric Society

Background Theoretically hydroxyethyl starch (HES) will givemore rapid recovery from shock, including in dengue shocksyndrome (DSS) and currently gained popularity for its lessdeleterious effects on renal function and blood coagulation.Objectives To compare the efficacy and safety ofHES 130/0.4 andRinger's lactate (RL) for shock recovery in children with DSS.Methods A randomized controlled study was performed on 39children admitted with DSS at Dr. Soetomo Hospital, Surabaya,between March and May 2007. Children were grouped intograde III (n=25) and grade IV (n=14) dengue hemorrhagicfever (DHF) according to the WHO criteria. Within eachgroup, subjects were randomly assigned to receive initial fluidresuscitation with either HES 130/0.4 (n=9 in the DHF grade IIIgroup, 10 in the DHF grade IV) or RL (n= 16 in the DHF gradeIII group, 4 in the DHF grade IV). Clinical and laboratory datawere collected to determine improvements in shock recovery andadverse reactions.Results In both the grades III and IV DHF, HES 130/0.4significantly decreased hemoglobin and hematocrit levels. Clinicalimprovements in pulse pressure and pulse rate were seen aftertreatment with HES 130/0.4 although these were statisticallyinsignificant if compared to the RL group. No differences in fluidrequirement and recurrent shock episodes were noted betweenthe RL and HES groups. No adverse reactions were found duringthe study.Conclusion HES 130/0.4 administration is effective and safe inchildren with DSS.

Early protection against measles infection in children Immunized with DPT-measles Vaccine at the Age of 4 Months Soegeng Soegijanto; Dwi Atmaji Soejono; Parwati Setiono B.
Paediatrica Indonesiana Vol 33 No 3-4 (1993): March - April 1993
Publisher : Indonesian Pediatric Society

A study on early immunization with DPT-measles vaccine at the age of 4 months, was earned out during 1990 - 1991 on 348 babies at the Health Centre of Surabaya regency. Three hundred and forty eight babies were served as control. The results of the study reported here were : (a) By giving a mixture of DPT and measles at an early age (4 months of age) the coverage of measles immunization could be increased to 21% - 27%. (b) 1'wenty two percents of measles cases could be prevented. (c) The geometric mean titer using hemagglutination inhibition test of the babies who got a mixture DP7~measles vaccination at an early age was significantly higher than that of the GMT HI test of babies who got measles vaccination at the age of 9 months ( z test, p <0, 05 ) . The result of analysis of data showed that an early immunization program using a mixture of DPT-measles could protect babies under 9 months and thus prevent them from measles infection.

In Vitro Study: Effect of Cobalt(II) Chloride Against Dengue Virus Type 1 in Vero Cells Teguh Hari Sucipto; Yovilianda Maulitiva Untoro; Harsasi Setyawati; Anisa Maharani; Novia Faridatus Sholihah; Siti Churrotin; Ilham Harlan Amarullah; Soegeng Soegijanto
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm30iss4pp316

Dengue virus (DENV) serotypes DENV-1 to DENV-4 are enveloped viruses that belong to the genus Flavivirus of the Flaviviridae. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is needed to develop to be an alternative drug for DENV virus. Cobalt(II) chloride have been used in the treatment and prevention of diseases of humans since ancient times. The aim of this study is to investigate the antiviral effects and Cytotoxicity of Cobalt(II) chloride. This compound was further investigated for its inhibitory effect on the replication of DENV-1 in Vero cells. Antiviral activity and Cytotoxicity measured by WST-1 assay. The IC50 value of the Cobalt(II) chloride for DENV-1 was 0.38 μg/ml. The cytotoxicity of Cobalt(II) chloride to Vero cell suggest that the CC50 value was 2.91 µg/ml The results of this study demonstrate the anti-dengue serotype 1 inhibitory activity of Cobalt(II) chloride was a high toxic compound.

UPDATE MANAGEMENT OF DENGUE COMPLICATION IN PEDIATRIC Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 2 No. 1 (2011)
Publisher : Institute of Topical Disease Universitas Airlangga

Dengue virus infection is one of the important health problems in Indonesia, although the mortality rate has been decreased but many dengue shock syndrome cases is very difficult to be solving handled. It might be due to nature course of dengue virus infection is very difficult to predict of the earlier time of severity occur. THE AIM To get idea to make update management of dengue complication in pediatric. MATERIAL AND METHOD Data were compiled from Dr. Soetomo Hospital Surabaya in 2009. The diagnosis of all cases was based on criteria WHO 1997 and PCR examination in Institute Tropical Disease for identified serotype of dengue virus infection. The unusual cases of dengue virus infection were treated following the new WHO protocol in 2009. RESULT There were only 3 cases with serotype DEN 1, consisted 2 cases had age 1–4 years and 1 had age 5–14 years. 2 cases showed a severe clinical performance as dengue shock syndrome and 1 case showed as unusual case of dengue virus infection. Three report cases of: a. Dengue hemorrhagic fever grade III which liver involvement and had bilateral pleural effusion; b. Dengue hemorrhagic grade III with liver involvement and encephalopathy; c. Dengue hemorrhagic grade III with liver involvement acute kidney injury, myocardial involvement and encephalopathy. All the patients were treated according to new edition WHO protocol and all of the involving organ recovered along with the improvement of the disease. CONCLUSION Update management of dengue complication pediatric should be learned carefully used for helping unusual cases of dengue virus infection.

THE MONTHLY CHANGING OF THE LOWEST POPULATION DENGUE VIRUS INFECTION IN PATIENT AT SOERYA HOSPITAL SIDOARJO IN 00 Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol. 2 No. 1 (2011)
Publisher : Institute of Topical Disease Universitas Airlangga

Background: Dengue virus infection is one of the important health problems in Indonesia, although the mortality rate has been decreased but many dengue shock syndrome cases is very difficult to be solving handled. To solve this problem, some factor that influence the prevalence of dengue virus infection should be studied. The Aim of Study: To detect some factor that maintain the higher case of dengue virus infection in patient at the Soerya Hospital Sepanjang, Sidoarjo. Material & Method: Study had been done at Soerya Hospital Sepanjang, Sidoarjo since January 1, 2007 until December 31, 2010. All cases suspected dengue virus infection in patient at soerya hospital were diagnosed based on WHO criteria in 1997 and PCR examination in ITD laboratory. The Result: In 2007, 2008, 2009, the monthly observation showed that decreasing cases of dengue virus infection in patient at Soerya Hospital had been found on September, but in 2010 this event had been found on November. Why this event to be change? It is suggested might be due to global warming in the world and the climate going to influence the environment sanitation. Interaction between agent host and environment becoming increase it might be due to the changing of climate can influence the growing population Aedes Aegyptie and Aedes Albopictus promoting to increase vector for transmit dengue virus infection. It is prominent in sub urban area, with have many peoples don't aware with the bad environment sanitation. And many peoples showed very dynamic for living until the idea good environment do not be thought. By this condition the monthly population dengue virus infection in patient at hospital are going to maintain higher more than six months than usually. On the year 2007, 2008, 2009 the lowest cases found on September. In 2010, the lowest cases had been found on November. The Conclusion: Global warming, increasing sub urban area which have many peoples don't aware with the bad environment sanitation and have highly dynamic peoples for getting some money for their life, could influence the higher cases dengue virus infection in patient at hospital more than 6 months.

THE CHANGING CLINICAL PERFORMANCE OF DENGUE VIRUS INFECTION IN THE YEAR 2009 Soegijanto, Soegeng; Susilowati, Helen; Mulyanto, Kris Cahyo; Hendrianto, Eryk; Yamanaka, Atushi
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 1 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Background: Dengue (DEN) virus, the most important arthropod-borne human pathogen, represents a serious public health threat. DEN virus is transmitted to humans by the bite of the domestic mosquito, Aedes aegypti, and circulates in nature as four distinct serological types DEN-1 to 4). The aim of Study: To identify Dengue Virus Serotype I which showed mild clinical performance in fiveyears before and afterward showed severe clinical performance. Material and Method: Prospective and analytic observational study had been done in Dr. Soetomo Hospital and the ethical clearance was conduct on January 01, 2009. The population of this research is all cases of dengue virus infection. Diagnosis were done based on WHO 1997. All of these cases were examined for IgM & IgG anti Dengue Virus and then were followed by PCR examination to identify Dengue Virus serotype. Result and Discussion: DEN 2 was predominant virus serotype with produced a spectrum clinical illness from asymptomatic, mild illness to classic dengue fever (DF) to the most severe form of illness (DHF). But DEN 1 usually showed mild illness. Helen at al (2009–2010) epidemiologic study of Dengue Virus Infection in Health Centre Surabaya and Mother and Child Health Soerya Sidoarjo found many cases of Dengue Hemorrhagic Feverwere caused by DEN 1 Genotype IV. Amor (2009) study in Dr. Soetomo Hospital found DEN 1 showed severe clinical performance of primary Dengue Virus Infection as Dengue Shock Syndrome two cases and one unusual case.Conclusion: The epidemiologic study of Dengue Virus Infection in Surabaya and Sidoarjo; in the year 2009 found changing predominant Dengue Virus Serotype from Dengue Virus II to Dengue Virus 1 Genotype IV which showed a severe clinical performance coincident with primary infection.

Co-Authors Abdul Latief Azis Alelo, Richardo Reynaldi Sakka Amaliah Labiqah, Amaliah Amor Peraten Ginting, Amor Peraten Anisa Maharani Arif Ansori, Arif Arif Nur Muhammad Ansori Aryati Aryati Atsushi Yamanaka, Atsushi Atsusi Yamanaka, Atsusi Atushi Yamanaka, Atushi Budi Setiawan Budi Utomo Desiana W Sari, Desiana W Desiana W. Sari, Desiana W. Desiana Wikanesthi, Desiana Dwi Atmaji Soejono Dyah Wikanesthi, Dyah Eiji Konichi, Eiji Eiji Konishi, Eiji Eryk Hendrianto Eva Chilvia, Eva Fahimah Martak Fedik A Rantam Harsasi Setyawati Hartati, Edim Hebert Adrianto Helen Susilowati, Helen Ilham Harlan Amarullah Ismoedijanto Kris Cahyo Mulyanto, Kris Cahyo Kris Cahyo Mulyatno, Kris Cahyo Lely Kurniasari, Lely Maharani, Anisa Masa Nori Kamioka, Masa Nori Masanori Kameoka, Masanori Ni Njoman Juliasih Novia Faridatus Sholihah Nuha, Zakiyathun Nur Ahwanah, Nur Nuryandari, Sufiandika Oedojo Soedirham Parwati Setiono Basuki Pemta Deka, Pemta Puspa Wardhani Reny Mareta Sari, Reny Mareta Risky Vitria Prasetyo Rizqidhana Juliana Putri S.Pd. M Kes I Ketut Sudiana . Sary, Dian Dwi Shifa Fauziyah Shuhai Ueda Siti Churotin, Siti Siti Churrotin, Siti Soetjipto Soetjipto Sri Subekti Sri Sumarsih Teguh Hari Sucipto, Teguh Hari Teguh Sucipto, Teguh Tomohiro Kotaki, Tomohiro Ueda, Shuhai Wardhani, Puspa Wibrianto, Aswandi Widodo Darmowandowo, Widodo Yoes Priyatna Yovilianda Maulitiva Untoro

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